Article
Plant Sciences
Yaqi An, Dong Wang, Jingxia Du, Xinwei Wang, Jianwei Xiao
Summary: The carbon dioxide emitted by human accounts for only a small fraction of global photosynthesis consumption, half of which is due to microalgae. The high efficiency of algae photosynthesis is attributed to the pyrenoid-based CO2-concentrating mechanism (CCM). The formation of pyrenoid which has a variety of Rubisco-binding proteins mainly depends on liquid-liquid phase separation (LLPS) of Rubisco, a CO2 fixing enzyme. The current research on the structure, assembly and application of Chlamydomonas reinhardtii pyrenoids is summarized in this article, providing new ideas for improving crop photosynthetic performance and yield.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Review
Plant Sciences
Elena V. Kupriyanova, Natalia A. Pronina, Dmitry A. Los
Summary: This review summarizes recent advances in the study of intracellular accumulation of inorganic carbon (C-i) in microalgae and cyanobacteria, revealing the third CO2-concentrating mechanism (CCM) in addition to the known CCM schemes in CAM and C-4 higher plants. CCM enables efficient CO2 fixation in the reductive pentose phosphate (RPP) cycle by coordinating carbonic anhydrases and CO2/HCO3- uptake systems. It functions as an add-on to the RPP cycle and as an important regulatory link in the interaction of dark and light reactions of photosynthesis.
Editorial Material
Biology
Britta Foerster
Summary: The structure that aids algae in photosynthesis under low carbon dioxide levels may also have a role to play in hyperoxia conditions.
Article
Biology
Peter Neofotis, Joshua Temple, Oliver L. Tessmer, Jacob Bibik, Nicole Norris, Eric Pollner, Ben Lucker, Sarathi M. Weraduwage, Alecia Withrow, Barbara Sears, Greg Mogos, Melinda Frame, David Hall, Joseph Weissman, David M. Kramer
Summary: The study shows that in Chlamydomonas reinhardtii, the pyrenoid can be strongly induced by hyperoxia, even in the presence of high levels of CO2 or bicarbonate. This suggests that the pyrenoid may be induced by a common product of photosynthesis specific to low CO2 or hyperoxia conditions. Additionally, evidence is presented for linkages between genetic variations in hyperoxia tolerance, H2O2 signaling, and pyrenoid morphologies.
Article
Multidisciplinary Sciences
M. Aguila Ruiz-Sola, Serena Flori, Yizhong Yuan, Gaelle Villain, Emanuel Sanz-Luque, Petra Redekop, Ryutaro Tokutsu, Anika Kuken, Angeliki Tsichla, Georgios Kepesidis, Guillaume Allorent, Marius Arend, Fabrizio Iacono, Giovanni Finazzi, Michael Hippler, Zoran Nikoloski, Jun Minagawa, Arthur R. Grossman, Dimitris Petroutsos
Summary: Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO2 conditions. Depletion of CO2 can drive photoprotection and carbon acquisition even in the absence of light, previously believed to be indispensable for these processes.
NATURE COMMUNICATIONS
(2023)
Review
Engineering, Marine
Jingyi Sun, Chunyan Zhao, Shuang Zhao, Wei Dai, Jinlin Liu, Jianheng Zhang, Juntian Xu, Peimin He
Summary: This paper provides a summary of the carbon concentrating mechanisms (CCM) in macroalgae, including important components and their functions. It introduces several indexes for evaluating the relative function of CCMs and summarizes methods for determining the carbon sequestration pathway, CCM responses under different carbon sources, and related genes involved in CCM operation. This work is important for understanding the CCMs of macroalgae and advancing research on the inorganic carbon utilization strategy of Ulva sp.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Plant Sciences
Liat Adler, Aranzazu Diaz-Ramos, Yuwei Mao, Krzysztof Robin Pukacz, Chenyi Fei, Alistair J. McCormick
Summary: This review discusses recent advances in understanding the pyrenoid-based CO2-concentrating mechanism (CCM) in Chlamydomonas reinhardtii and the engineering progress in C3 plants. Key findings include the potential benefits of Rubisco condensation and the minimal components required for a functional CCM.
Article
Electrochemistry
Adnan Sayegh, Luca A. Perego, Marc Arderiu Romero, Louis Escudero, Jerome Delacotte, Manon Guille-Collignon, Laurence Grimaud, Benjamin Bailleul, Frederic Lemaitre
Summary: This study focuses on extracting energy from oxygenic photosynthesis by rerouting electron flow using intact photosynthetic organisms to generate photocurrent. Researchers found that some quinones can interact with the embedded photosynthetic chain, but the balance between toxicity and bioelectricity production needs to be considered.
Article
Biochemistry & Molecular Biology
Juliana Artier, Ross M. Walker, Neil T. Miller, Minquan Zhang, G. Dean Price, Robert L. Burnap
Summary: The study focused on investigating the role of conserved histidine and cysteine residues in the CupB protein in cyanobacteria using a Synechococcus PCC7942 expression system. Results showed that the most severe defects in activity among the targeted residues were due to a substitution of CupB-His86, and proteomic analysis revealed that certain amino acid substitution mutations of CupB induced bicarbonate uptake proteins to a greater extent.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Plant Sciences
Ashwani K. Rai, Timothy Chen, James Moroney
Summary: Chlamydomonas reinhardtii can grow photosynthetically using CO2, with the CO2 concentrating mechanism (CCM) playing a crucial role. Research suggests that mitochondrial carbonic anhydrases CAH4 and CAH5 are involved in maintaining optimal photosynthesis in C. reinhardtii.
Review
Biochemistry & Molecular Biology
James Barrett, Philipp Girr, Luke C. M. Mackinder
Summary: Pyrenoids are non-membrane bound organelles found in chloroplasts of algae and hornwort plants that play a crucial role in CO2 concentration and photosynthesis. Liquid-liquid phase separation is the general principle underlying pyrenoid formation, accelerating global carbon fixation. Conducting further research on pyrenoid assembly and regulation could provide valuable insights for enhancing photosynthetic performance in crops.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Review
Plant Sciences
Indu Santhanagopalan, Rachel Wong, Tanya Mathur, Howard Griffiths
Summary: This review examines the inducible carbon concentration mechanism (CCM) in Chlamydomonas reinhardtii, focusing on the relationship between external CO2 limitation, energetic supply, and inorganic carbon demand. It suggests that the chloroplast plays a crucial role in photosynthesis and regulates CCM induction by sending retrograde signals to the nucleus. The hypothesis is that retrograde signals associated with photorespiration and/or light stress are linked to CCM induction.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Tom Z. Emrich-Mills, Gary Yates, James Barrett, Philipp Girr, Irina Grouneva, Chun Sing Lau, Charlotte E. Walker, Tsz Kam Kwok, John W. Davey, Matthew P. Johnson, Luke C. M. Mackinder
Summary: The development of a recombineering pipeline allowed for successful parallel cloning of genes from a Chlamydomonas bacterial artificial chromosome collection, with an overall cloning success rate of 77%. The cloning success was independent of gene size and complexity, with cloned genes reaching sizes as large as 23 kb. This technology has the potential to open up new possibilities in algal and plant research.
Article
Biotechnology & Applied Microbiology
Eun Ju Yun, Guo-Chang Zhang, Christine Atkinson, Stephan Lane, Jing-Jing Liu, Donald R. Ort, Yong-Su Jin
Summary: The Chlamydomonas reinhardtii cia5 mutant can produce substantial amounts of glycolate, a high-value cosmetic ingredient, under ambient air conditions. Metabolomic analysis revealed significant differences in metabolic profiles between the cia5 mutant and the wild type strain during the photoautotrophic phase, highlighting the potential for producing glycolate and engineering alternative pathways for glycolate metabolism in C. reinhardtii.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Marius Arend, Yizhong Yuan, M. Aguila Ruiz-Sola, Nooshin Omranian, Zoran Nikoloski, Dimitris Petroutsos
Summary: Using a transcriptomic data compendium of Chlamydomonas reinhardtii, we reconstructed a gene regulatory network and identified transcriptional regulators of photoprotection. Our study showed that the CCM regulator LCR1 also controls photoprotection, and QER7, a Squamosa Binding Protein, suppresses photoprotection and CCM-gene expression under the control of Phototropin. This study provides insights into gene expression regulation in microalgae.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Nicky Atkinson, Doreen Feike, Luke C. M. Mackinder, Moritz T. Meyer, Howard Griffiths, Martin C. Jonikas, Alison M. Smith, Alistair J. McCormick
PLANT BIOTECHNOLOGY JOURNAL
(2016)
Editorial Material
Biology
Moritz Meyer, Howard Griffiths
Review
Plant Sciences
Moritz T. Meyer, Alistair J. McCormick, Howard Griffiths
CURRENT OPINION IN PLANT BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Luke C. M. Mackinder, Moritz T. Meyer, Tabea Mettler-Altmann, Vivian K. Chen, Madeline C. Mitchell, Oliver Caspari, Elizabeth S. Freeman Rosenzweig, Leif Pallesen, Gregory Reeves, Alan Itakura, Robyn Roth, Frederik Sommer, Stefan Geimer, Timo Muehlhaus, Michael Schroda, Ursula Goodenough, Mark Stitt, Howard Griffiths, Martin C. Jonikas
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Plant Sciences
Madeline C. Mitchell, Gergana Metodieva, Metodi V. Metodiev, Howard Griffiths, Moritz T. Meyer
JOURNAL OF EXPERIMENTAL BOTANY
(2017)
Article
Plant Sciences
Oliver D. Caspari, Moritz T. Meyer, Dimitri Tolleter, Tyler M. Wittkopp, Nik J. Cunniffe, Tracy Lawson, Arthur R. Grossman, Howard Griffiths
JOURNAL OF EXPERIMENTAL BOTANY
(2017)
Editorial Material
Plant Sciences
Howard Griffiths, Moritz T. Meyer, Rosalind E. M. Rickaby
JOURNAL OF EXPERIMENTAL BOTANY
(2017)
Article
Plant Sciences
Nicky Atkinson, Nuno Leitao, Douglas J. Orr, Moritz T. Meyer, Elizabete Carmo-Silva, Howard Griffiths, Alison M. Smith, Alistair J. McCormick
Article
Plant Sciences
Madeline C. Mitchell, Moritz T. Meyer, Howard Griffiths
Article
Genetics & Heredity
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman, Martin C. Jonikas
Article
Plant Sciences
Myriam M. M. Goudet, Douglas J. Orr, Michael Melkonian, Karin H. Muller, Moritz T. Meyer, Elizabete Carmo-Silva, Howard Griffiths
Article
Plant Sciences
Monica Mejia-Chang, Casandra Reyes-Garcia, Ulli Seibt, Jessica Royles, Moritz T. Meyer, Glyn D. Jones, Klaus Winter, Miguel Arnedo, Howard Griffiths
Summary: This study used stable isotopes to investigate the distributions of CAM and C-3 epiphytic bromeliads across an altitudinal gradient in western Panama and their water balance. The results demonstrated significant differences in leaf water among different altitudes and seasons.
FUNCTIONAL PLANT BIOLOGY
(2021)
Article
Plant Sciences
Shan He, Hui-Ting Chou, Doreen Matthies, Tobias Wunder, Moritz T. Meyer, Nicky Atkinson, Antonio Martinez-Sanchez, Philip D. Jeffrey, Sarah A. Port, Weronika Patena, Guanhua He, Vivian K. Chen, Frederick M. Hughson, Alistair J. McCormick, Oliver Mueller-Cajar, Benjamin D. Engel, Zhiheng Yu, Martin C. Jonikas
Article
Multidisciplinary Sciences
Moritz T. Meyer, Alan K. Itakura, Weronika Patena, Lianyong Wang, Shan He, Tom Emrich-Mills, Chun S. Lau, Gary Yates, Luke C. M. Mackinder, Martin C. Jonikas