期刊
JOURNAL OF EXPERIMENTAL BIOLOGY
卷 220, 期 8, 页码 1481-1489出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jeb.151506
关键词
GIFT; miR-29a; Stearoyl-CoA desaturase; Saturated fatty acid
类别
资金
- Central Public-interest Scientific Institution Basal Research Fund, CAFS [2017HY-XKQ0202]
- Special Fund for National Natural Science Foundation of China [31502143]
- National Science & Technology Program [2012BAD26B03-1]
MicroRNAs (miRNAs) are small non-coding RNAs that regulate target gene expression by binding to the 3' untranslated region (3' UTR) of the target mRNA. MiRNAs regulate a large variety of genes, including those involved in liver biology and disease. Here, we report for the first time that miR-29a post-transcriptionally regulates stearoyl-CoA desaturase (SCD) by binding to its 3' UTR in genetically improved farmed tilapia (GIFT), Oreochromis niloticus, as shown by a 3' UTR luciferase reporter assay. miR-29a antagomir treatment in vivo resulted in significant upregulation of SCD expression. We found that miR-29a expression was negatively correlated with SCD expression in GIFT liver. Inhibition of miR-29a led to a significant increase in SCD expression on day 60 induced by a saturated fatty acid diet, thereby increasing conversion of 16: 0 and 18: 0 to 16: 1 and 18: 1, respectively, and activating serum insulin, which would favor glucose and lipid uptake by the liver. These results indicate that miR29a regulates SCD levels by binding to its 3' UTR, and this interaction affects saturated fatty acid stress induction and insulin and lipid accumulation in serum. Our results suggest that miR-29a is critical in regulating lipid metabolism homeostasis in GIFT liver, and this might provide a basis for understanding the biological processes and therapeutic intervention encountered in fatty liver.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据