Characterization and cytotoxicity of PLGA nanoparticles loaded with formononetin cyclodextrin complex

The study aimed to prepare and characterize the formononetin-2-hydroxypropy1-beta-cyclodextrin inclusion complex loaded PLGA nanoparticles (FMN-2-HP beta-CD-NP). The neutralization agitation method was used to prepare FMN-2-HP beta-CD inclusion complex. Phase solubility study demonstrated the formation of A(L) type inclusion complex. The inclusion stability constant was 2116 +/- 73 M-1. The PLGA nanoparticles were prepared by solid/oil/water (S/O/W) technology and theirs in vitro properties were studied. Experiments of NMR, FTIR, XRD and SEM confirmed the formation of FMN-2-HP beta-CD inclusion complex. Transmission electron microscopy (TEM) showed the spherical morphology of FMN-2-HP beta-CD-NP. The average particle size was 210.23 +/- 3.4 nm, the polydispersity index was 0.108 +/- 0.02, the Zeta potential was -26.25 +/- 0.42 mV and the mean encapsulation efficiency was 91.97 +/- 0.79%. The in vitro release study indicated that FMN-2-HP beta-CD-NP had a sustained release effect. The in vitro cytotoxicity showed that FMN-2-HP beta-CD-NP remained anticancer activity in Hela and MCF-7 tumor cells compared to free FMN. In conclusion, the novel delivery system of drug-in-cyclodextrin-in-nanoparticles was a promising carrier for poor lipophilic and poor hydrophilic drugs. (C) 2017 Elsevier B.V. All rights reserved.