Article
Dentistry, Oral Surgery & Medicine
A. Vijaykumar, S. H. Root, M. Mina
Summary: The study found that treatment with WNT3a did not induce premature differentiation of odontoblasts, but instead supported the survival of undifferentiated cells and promoted the formation of preodontoblasts in dental pulp, facilitating their rapid transition into differentiated odontoblasts. Additionally, WNT3a was also found to promote osteogenesis in dental pulp cultures. These findings provide valuable insights for improving vital pulp therapy and dentin regeneration treatments.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xinghong Luo, Jingyao Yin, Shenghong Miao, Weiqing Feng, Tingting Ning, Shuaimei Xu, Shijiang Huang, Sheng Zhang, Yunjun Liao, Chunbo Hao, Buling Wu, Dandan Ma
Summary: The study revealed that mTORC1 plays a crucial role in odontoblast proliferation and mineralization, and promotes odontoblast mineralization through the p53 pathway. By inhibiting p53 alone or both mTORC1 and p53, it was demonstrated that p53 acts downstream of mTORC1 in regulating odontoblast mineralization.
Article
Cell & Tissue Engineering
Jing Li, Youming Zhu, Na Li, Tao Wu, Xianyu Zheng, Boon Chin Heng, Duohong Zou, Jianguang Xu
Summary: By overexpressing ETV2, human dental pulp stem cells (DPSCs) showed significantly enhanced differentiation into endothelial cells, with increased angiogenic potential both in vitro and in vivo. Proteomic analysis revealed upregulation of VEGF receptors, indicating enhanced VEGF signaling. These results suggest that DPSCs could be a promising cell source for tissue engineering applications.
CELL TRANSPLANTATION
(2021)
Article
Cell Biology
Shijian Deng, Linlin Fan, Yunfei Wang, Qi Zhang
Summary: This study revealed the involvement of beta-catenin in regulating odontoblast polarization, influencing the secretion of mineralization proteins, and ultimately affecting dentin formation and mineralization.
JOURNAL OF MOLECULAR HISTOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Jie Liu, Xiaoling Wei, Junlong Hu, Xiaohan Tan, Xiaocui Kang, Li Gao, Ning Li, Xin Shi, Mengtong Yuan, Weiping Hu, Mingyue Liu
Summary: In this study, it was found that high concentrations of C5a can enhance odontoblast differentiation and increase DSP levels in DPSCs, while low concentrations of C5a have inhibitory effects. All six groups formed mineralized nodules after 28 days of culture.
Article
Dentistry, Oral Surgery & Medicine
J. Fu, H. Zheng, Y. Xue, R. Jin, G. Yang, Z. Chen, G. Yuan
Summary: WWP2 promotes odontoblastic differentiation by monoubiquitinating KLF5, leading to the regulation of odontoblast marker gene expression.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yuka Kato, Satoshi Yokose
Summary: The study confirms that oxytocin stimulates odontoblastlike cell differentiation and promotes dentin formation, indicating that it regulates odontoblastlike cell differentiation via the OT receptor and the Wnt canonical pathway is closely related to these effects.
JOURNAL OF ENDODONTICS
(2021)
Article
Biophysics
Vinu Vijayan, Rachita Lakra, Purna Sai Korrapati, Manikantan Syamala Kiran
Summary: In this study, a rare earth lanthanum oxide nanoparticle reinforced collagen biomatrix was developed and evaluated for its bioactivity. The results showed that the biomatrix had good structural integrity, mechanical strength, and resistance to degradation. The lanthanum oxide nanoparticle promoted endothelial cell activation and angiogenesis, and enhanced cell migration. Therefore, this study provides a new approach for developing rare earth-based biomaterials with biological effects.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Dentistry, Oral Surgery & Medicine
M. Watanabe, M. Okamoto, S. Komichi, H. Huang, S. Matsumoto, K. Moriyama, J. Ohshima, S. Abe, M. Morita, M. Ali, K. Takebe, I. Kozaki, A. Fujimoto, K. Kanie, R. Kato, K. Uto, M. Ebara, A. Yamawaki-Ogata, Y. Narita, Y. Takahashi, M. Hayashi
Summary: This study investigates the effects of functional peptides derived from the S100A8 protein on dental pulp tissue. It found that one of the peptides can promote tertiary dentin formation and inhibit inflammatory responses. These findings provide important insights for the development of next-generation biological vital pulp therapies.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Cell Biology
Ryan Pasiewicz, Yessenia Valverde, Raghuvaran Narayanan, Ji-Hyun Kim, Muhammad Irfan, Nam-Seob Lee, Anne George, Lyndon F. Cooper, Satish B. Alapati, Seung Chung
Summary: The study revealed that C5a plays a positive role in the odontogenic differentiation of DPSCs, with C5a receptor inhibition resulting in a significant decrease in odontogenic differentiation genes. The hypoxic condition can reverse this effect.
CONNECTIVE TISSUE RESEARCH
(2022)
Article
Materials Science, Multidisciplinary
Abhishek Singh, Satish Kumar, Tusar Kanta Acharya, Chandan Goswami, Luna Goswami
Summary: This study developed a polysaccharide-based biomaterial with nanohydroxyapatite as a filler. The physicochemical characterization showed that the material is biocompatible towards nonosteoblasts and osteoblasts cells and exhibit no cytotoxic effects. Furthermore, this biomaterial enhances the in vitro biomineralization of osteoblast cells under osteoinductive conditions.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kristi Rothermund, Tia C. Calabrese, Fatima N. Syed-Picard
Summary: This study demonstrates the use of scaffold-free dental pulp stem cell constructs as models for oral diseases and highlights the diverse effects of lipopolysaccharide (LPS) derived from different bacterial species on the differentiation and function of dental pulp stem cells.
JOURNAL OF ENDODONTICS
(2022)
Article
Engineering, Biomedical
Liangle Liu, Ziyue Peng, Chenjian Wang, Chengqiang Wang, Chun Liu, Lixin Zhu, Chengxuan Tang
Summary: In this study, a three-dimensional system using CaO2/poly(lactic-co-glycolic acid) microspheres suspended in a hydrogel was developed to generate oxygen and support cell growth under hypoxic conditions, promoting stem cell survival and growth following implantation.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2021)
Article
Pharmacology & Pharmacy
Ihn Han, Juie Nahushkumar Rana, Ji-Hye Kim, Eun Ha Choi, Youngsun Kim
Summary: This study evaluated the osteogenic potential of non-thermal biocompatible plasma (NBP)-treated chitosan scaffolds. The results showed that NBP-treated chitosan scaffolds demonstrated significant improvements in cell viability and differentiation.
Review
Biochemistry & Molecular Biology
Jon Luzuriaga, Yurena Polo, Oier Pastor-Alonso, Beatriz Pardo-Rodriguez, Aitor Larranaga, Fernando Unda, Jose-Ramon Sarasua, Jose Ramon Pineda, Gaskon Ibarretxe
Summary: Human dental pulp stem cells (hDPSCs) are a promising stem cell type for regenerative therapies due to their ability to grow without serum and potential for autologous grafts. This review discusses the advantages of hDPSCs for neuroregenerative cell therapies, exploring their embryonic origin, postnatal niche, differentiation potential, neuronal differentiation issues, neuroprotective and immunomodulatory capacities, as well as their integration with scaffold materials to enhance functionality in injured nervous systems. Perspectives on current and future applications of hDPSCs in neuroregenerative cell therapies are also provided.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Na-Yeon Kim, Hee-Jin Kim, Kyung-San Min
Summary: This study compared the efficacy of several passive ultrasonic irrigation (PUI) systems in removing intracanal medicament from curved root canals. The results showed that the PUI system made of nickel-titanium alloy with small dimensions, such as Endosonic Blue, had the highest removal efficacy, while CK showed the lowest efficacy. Therefore, these small-sized PUI systems have advantages in cleaning curved root canals.
AUSTRALIAN ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yoorina Choi, Yun-Chan Hwang, Mi-Kyung Yu, Kwang-Won Lee, Kyung-San Min
Summary: This study evaluated the effect of barium titanate (BT) on the dielectricity, radiopacity, and biological properties of tricalcium silicate (C3S). C3S/BT samples with varying proportions of BT (0, 20, 40, and 60 wt%) were prepared and tested. The results showed that higher proportions of BT led to increased dielectric constant and satisfactory radiopacity. The cytocompatibility tests showed no significant differences among the groups. New bone formation and expression of specific proteins were observed in samples with 40-60 wt% BT.
DENTAL MATERIALS JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Mi-Ah Kim, Vinicius Rosa, Kyung-San Min
Summary: This study investigated the antibacterial efficacy and cytotoxicity of two graphene derivatives - graphene oxide (GO) and reduced graphene oxide (RGO) - against Enterococcus faecalis in biofilms formed on hydroxyapatite discs. GO and RGO had similar antibacterial effects on E. faecalis biofilms. SEM images showed the ability of GO and RGO to disrupt E. faecalis, while GO had significantly higher cytotoxicity than RGO. The results suggest that RGO has the potential to be used as a more effective disinfecting agent than GO.
DENTAL MATERIALS JOURNAL
(2023)
Article
Biochemical Research Methods
Toan V. Phan, Yamin Oo, Khurshid Ahmed, Teerapat Rodboon, Vinicius Rosa, Supansa Yodmuang, Joao N. Ferreira
Summary: Hyposalivation and severe dry mouth syndrome are common complications in HNC patients after radiation therapy. Conventional treatment with sialogogues is limited due to the destruction of the salivary gland's secretory parenchyma and reduced regenerative potential. Adipose mesenchymal stem cells (AdMSC) show promise in addressing this issue.
Article
Biochemical Research Methods
Joana Rita Oliveira Faria Marques, Patricia Gonzalez-Alva, Ruby Yu-Tong Lin, Beatriz Ferreira Fernandes, Akhilanand Chaurasia, Nileshkumar Dubey
Summary: Cancer treatment development is complex due to tumor heterogeneity and inter-patient variations. Traditional 2D cell culture is insufficient to mimic tumor-specific architecture, while 3D culture techniques and bioprinting have shown promise in bridging the gap.
Article
Dentistry, Oral Surgery & Medicine
Arief Cahyanto, Marcos Vinicius Surmani Martins, Otavio Bianchi, Deepa Perumbodathu Sudhakaran, Nikolaos Sililkas, Sergio Graniero Echeverrigaray, Vinicius Rosa
Summary: This study investigated the effects of graphene oxide (GO) on the reliability and lifetime of PMMA. The results showed that GO improved the fatigue resistance and predicted lifetime of PMMA, but had no significant effect on its Weibull parameters.
Article
Dentistry, Oral Surgery & Medicine
A. Cahyanto, P. Rath, T. X. Teo, S. S. Tong, R. Malhotra, B. N. Cavalcanti, L. Z. Lim, K. S. Min, D. Ho, W. F. Lu, V. Rosa
Summary: This study used Taguchi's methods and genetic algorithms to optimize the properties of calcium silicate cements, achieving personalized treatment for patients' specific needs. By simultaneously analyzing multiple input factors, the study successfully designed cement formulations with predicted properties, which were experimentally validated. The study also demonstrated the bioactivity of the cement in dental pulp stem cells.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ji-Yoon Shin, Mi-Ah Kim, Hee-Jin Kim, Prasanna Neelakantan, Mi-Kyung Yu, Kyung-San Min
Summary: This study compared the effects of machine-assisted irrigation on biofilm removal and apical extrusion of sodium hypochlorite (NaOCl) using a 3D printed dentin-insert model. The results showed that ultrasonic agitation with EDDY and Endosonic Blue significantly reduced the biofilms and prevented NaOCl extrusion beyond the root apex, with EDDY demonstrating better removal efficacy.
JOURNAL OF ORAL SCIENCE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Mi-Ah Kim, Kyung-San Min
Summary: The purpose of this study was to investigate the antibacterial activity of apigenin and its synergistic effect with reduced graphene oxide (RGO) in treating E. faecalis biofilms. The results indicated that the combined use of apigenin and RGO could effectively eliminate E. faecalis biofilms, providing a potential strategy for endodontic disinfection.
JOURNAL OF ORAL SCIENCE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Hee-Jin Kim, Kyung-San Min
Summary: In addition to pathogenic teeth associated with cysts, the roots of adjacent teeth are often included in the cystic cavity. Whether these teeth require elective endodontic treatment followed by cystic enucleation remains unclear. In the case presented herein, we aimed to preserve the pulp of the teeth included in the cystic lesion. Unfortunately, the sensibility of the included teeth was negative after endodontic surgery, including cystic enucleation. However, the sensibility recovered after 1 year and was maintained throughout a 4.5-year follow-up. Therefore, we suggest that elective endodontic treatment of the included teeth should be avoided, and further research should be conducted regarding this issue.
EUROPEAN ENDODONTIC JOURNAL
(2023)
Article
Materials Science, Biomaterials
Nileshkumar Dubey, Juliana S. Ribeiro, Zhaocheng Zhang, Jinping Xu, Jessica A. Ferreira, Liu Qu, Ling Mei, J. Christopher Fenno, Anna Schwendeman, Steven P. Schwendeman, Jacques E. Nor, Marco C. Bottino
Summary: Regenerative endodontics is a paradigm shift in treating necrotic young permanent teeth. This study developed a hydrogel system that combines antimicrobial and angiogenic properties while minimizing cell toxicity. The engineered hydrogels demonstrated antibiotic-dependent inhibition of bacterial growth and enhanced angiogenesis in vitro and in vivo. This research highlights the clinical potential of antibiotic-eluting gelatin methacryloyl hydrogel for regenerative endodontics.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Dentistry, Oral Surgery & Medicine
Shiyao Liu, Ahmed El-Angbawi, Ruidong Ji, Vinicius Rosa, Nick Silikas
Summary: The objective of this study was to investigate the effects of adding graphene to orthodontic fixed retainer adhesives on colour and optical transmission. The results showed that adding graphene significantly darkened the adhesive colour and reduced translucency, but had no adverse effect on degree of conversion. The optical transmission of the adhesive decreases with increasing graphene concentration.
EUROPEAN JOURNAL OF ORAL SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Erfan Rezvani Ghomi, Rajamani Lakshminarayanan, Vijila Chellappan, Navin Kumar Verma, Amutha Chinnappan, Rasoul Esmaeely Neisiany, Kottaiswamy Amuthavalli, Zhi Sheng Poh, Brandon Han Siang Wong, Nileshkumar Dubey, Roger Narayan, Seeram Ramakrishna
Summary: This study demonstrates that aligned PCL/gelatin mats containing epsilon-PL have broad antimicrobial activity and excellent biocompatibility, making them promising for potential use in wound dressings.
ADVANCED FIBER MATERIALS
(2023)
Review
Engineering, Biomedical
Harish K. Handral, Vaishali P. Natu, Tong Cao, Jerry Y. H. Fuh, Gopu Sriram, Wen F. Lu
BIO-DESIGN AND MANUFACTURING
(2022)