Review
Cell Biology
Venkata Suresh Venkataiah, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Yusuke Kakiuchi, Masato Nakano, Shigeto Suzuki, Keisuke Handa, Masahiro Saito
Summary: Bone tissue engineering involves combining MSCs with a biocompatible scaffold to aid in bone regeneration and recovery. While promising results have been seen in clinical trials, regenerating large bone defects remains a challenge.
Article
Materials Science, Biomaterials
Jinsub Han, Sangbae Park, Jae Eun Kim, Byeongjoo Park, Yeonggeol Hong, Jae Woon Lim, Seung Jeong, Hyunmok Son, Hong Bae Kim, Hoon Seonwoo, Kyoung-Je Jang, Jong Hoon Chung
Summary: Developing an efficient and functional bone regeneration scaffold is challenging. A scaffold-on-a-chip device was developed to aid with the evaluation process. This device mimics the microenvironment experienced by a transplanted bone scaffold, allowing for monitoring of cell behavior at the blood-scaffold interphase.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Jinsub Han, Sangbae Park, Jae Eun Kim, Byeongjoo Park, Yeonggeol Hong, Jae Woon Lim, Seung Jeong, Hyunmok Son, Hong Bae Kim, Hoon Seonwoo, Kyoung-Je Jang, Jong Hoon Chung
Summary: Developing an efficient and functional bone regeneration scaffold is challenging. A scaffold-on-a-chip device was developed as a platform for evaluation. The device mimics the microenvironment experienced by a transplanted bone scaffold and allows for monitoring of cell behavior. MC3T3-E1 cells were cultured with different scaffold inserts to evaluate their osteoconductivity and osteoinductivity. The results showed that the platform effectively evaluated the properties of the scaffolds. This proposed platform can replace animal models and contribute to the study of bone regeneration.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Engineering, Biomedical
Kenichi Arai, Takahiro Kitsuka, Koichi Nakayama
Summary: The safety and efficacy of new drugs are typically tested in animals, but differences in drug responses and potential cardiac side effects have led to many drug development projects being discontinued. Alternative methods to animal testing are needed. Tissue engineering techniques can create three-dimensional cardiac constructs from hiPSC-CMs, providing a new approach for drug screening.
Article
Engineering, Biomedical
Anna Gryadunova, Jesil Kasamkattil, Max Hans Peter Gay, Boris Dasen, Karoliina Pelttari, Vladimir Mironov, Ivan Martin, Stefan Schaeren, Andrea Barbero, Olga Krupkova, Arne Mehrkens
Summary: This study demonstrates the potential of scaffold-free spheroids derived from human nasal chondrocytes for nucleus pulposus regeneration, showing good injectability and biochemical properties under suitable culture conditions, making it a promising strategy for degenerative disc disease treatment.
ACTA BIOMATERIALIA
(2021)
Article
Cell & Tissue Engineering
Siddharth Shanbhag, Salwa Suliman, Samih Mohamed-Ahmed, Carina Kampleitner, Mohamed Nageeb Hassan, Patrick Heimel, Toni Dobsak, Stefan Tangl, Anne Isine Bolstad, Kamal Mustafa
Summary: Three-dimensional spheroid culture can promote osteogenic differentiation of bone marrow mesenchymal stromal cells, while 3D printing allows for customized scaffolds for complex bone defects. This study compared the potential of human BMSC cultured as 2D monolayers or 3D spheroids in constructs for bone regeneration. Results showed a trend for superior in vitro mineralization with 3D BMSC, but similar in vivo performance between 2D and 3D BMSC, indicating PLATMC-HPLG constructs as promising scaffolds for bone tissue engineering.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Siddharth Shanbhag, Carina Kampleitner, Samih Mohamed-Ahmed, Mohammed Ahmad Yassin, Harsh Dongre, Daniela Elena Costea, Stefan Tangl, Andreas Stavropoulos, Anne Isine Bolstad, Salwa Suliman, Kamal Mustafa
Summary: Three-dimensional spheroid culture can enhance osteogenic differentiation and bone regeneration capacity of MSC, showing potential for clinical applications of GPC and BMSC. Combination of HPLG and PLATMC as scaffolds is promising for bone tissue engineering applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Wentao Wang, Xiaolong Liang, Kai Zheng, Gaoran Ge, Xu Chen, Yaozeng Xu, Jiaxiang Bai, Guoqing Pan, Dechun Geng
Summary: Exosomes, small vesicles with the ability of vesicle-mediated intercellular signal transmission, have played an important role in bone tissue engineering. Compared to cells, exosomes have the advantage of cell-free therapy, which can promote bone regeneration and overcome immune rejection.
MATERIALS TODAY BIO
(2022)
Article
Materials Science, Multidisciplinary
Changsong Zhou, Shenghui Su, Jiwen Fan, Jiuzao Lin, Xiaoyong Wang
Summary: In this study, PLGA/Si3N4 composite nanofiber scaffolds were successfully prepared and showed promising potential for bone regeneration due to their enhanced mechanical strength and biological activity.
FRONTIERS IN MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Abdullah Al-Ani, Derek Toms, Saud Sunba, Kayla Giles, Yacine Touahri, Carol Schuurmans, Mark Ungrin
Summary: The focus of the research is on developing size-controlled RPE microtissues suitable for injection delivery to improve the survival and integration of transplanted RPE cells, while also retaining the simplicity of production and delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Iratxe Macias, Natividad Alcorta-Sevillano, Arantza Infante, Clara Rodriguez
Summary: Bone damage leading to bone loss can be caused by a variety of reasons, including infections, diseases, trauma, or surgery. Current clinical bone surrogates have limitations, leading to extensive research into new therapies for skeletal regeneration of bone defects, such as stimulating host cells or using scaffolds, biomolecules, and stem cells to promote bone healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Minglei Yang, Jiafei Chen, Yi Chen, Weikang Lin, Hai Tang, Ziwen Fan, Long Wang, Yunlang She, Feng Jin, Lei Zhang, Weiyan Sun, Chang Chen
Summary: A scaffold-free modular strategy for tracheal engineering is developed, which constructs a complex tracheal substitute by laminating a cartilage sheet and a temperature-sensitive epithelium sheet. This substitute exhibits native-like structure and function, showing great clinical potential for tracheal reconstruction.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Hao Huang, Anchun Yang, Jinsheng Li, Tong Sun, Shangke Yu, Xiong Lu, Tailin Guo, Ke Duan, Pengfei Zheng, Jie Weng
Summary: This study improved the porosity and crystallinity of porous hydroxyapatite scaffolds by adjusting the solid content and sintering temperature. Further mineralization significantly enhanced the biological activity of the composite scaffold. In vivo results showed that the multigradient scaffold filled with mineralized hydroxyapatite spheres exhibited higher vascularization ability and a continuous decrease in osteogenic capability over time.
REGENERATIVE BIOMATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
D. G. Soares, E. A. F. Bordini, E. S. Bronze-Uhle, F. B. Cassiano, I. S. P. Silva, M. O. Gallinari, H. R. Matheus, J. M. Almeida, L. T. A. Cintra, J. Hebling, C. A. de Souza Costa
Summary: This study evaluated the bioactive potential of a simvastatin (SV)-releasing chitosan-calcium-hydroxide (CH-Ca) scaffold for dentin regeneration. Results showed that the CH-Ca scaffold combined with SV could promote odontoblastic differentiation of dental pulp cells (DPCs) in vitro and in vivo, inducing overexpression of odontoblastic markers and intense mineralization. The scaffold also had a chemoattractant effect on DPCs and enhanced mineralized matrix deposition, showing promise for regenerative dentistry applications.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Materials Science, Multidisciplinary
Jiaolong Wang, Qin Zhao, Shihang Zheng, Jinyang Wang, Liangliang Fu, Can Wang, Zifan Zhao, Yufeng Zhang
Summary: Macrophages play a crucial role in bone regeneration, and the CD301b+ macrophages exhibit significant effects in the osteoinduction of calcium phosphate ceramics, promoting the osteogenic differentiation of mesenchymal stem cells and potentially serving as a novel therapeutic target for bone regeneration.
APPLIED MATERIALS TODAY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Tomoyuki Iwata, Noriyoshi Mizuno, Takayoshi Nagahara, Eri Kaneda-Ikeda, Mikihito Kajiya, Shinya Sasaki, Katsuhiro Takeda, Mari Kiyota, Ryoichi Yagi, Tsuyoshi Fujita, Hidemi Kurihara
Summary: This study investigated the regulatory genes induced by periodontal regenerative cytokines using MSCs, identifying miR-628-5p as a negative regulator of MSC stemness during periodontal regeneration. Periodontal regenerative cytokines act as suppressors of miR-628-5p to support periodontal regeneration, indicating the importance of selecting effective cytokines for different MSCs based on miRNA profiling in advancing regenerative therapies.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Cell & Tissue Engineering
Tomoya Ogawa, Mikihito Kajiya, Susumu Horikoshi, Hiroki Yoshii, Mai Yoshino, Souta Motoike, Shin Morimoto, Hisakatsu Sone, Tomoyuki Iwata, Kazuhisa Ouhara, Shinji Matsuda, Noriyoshi Mizuno
Summary: The study suggests that MSCs treated with C-MSCsy can retain their immunosuppressive and osteogenic properties after cryopreservation, providing a promising off-the-shelf cell preparation for bone regenerative therapy.
REGENERATIVE THERAPY
(2022)
Article
Cell & Tissue Engineering
Hisakatsu Sone, Mikihito Kajiya, Katsuhiro Takeda, Shinya Sasaki, Susumu Horikoshi, Souta Motoike, Shin Morimoto, Hiroki Yoshii, Mai Yoshino, Tomoyuki Iwata, Kazuhisa Ouhara, Shinji Matsuda, Noriyoshi Mizuno
Summary: Periodontal tissue regeneration can be achieved by transplanting clumps of mesenchymal stem cells (MSCs) and extracellular matrix (ECM) complexes (C-MSCs) without artificial scaffold. In a rat model, C-MSCs transplantation successfully induced periodontal tissue regeneration, with the donor cells directly involved in tissue formation. This study demonstrates a promising scaffold-free MSCs transplantation strategy for periodontal disease.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Cell & Tissue Engineering
Daisuke Kamiya, Nana Takenaka-Ninagawa, Souta Motoike, Mikihito Kajiya, Teppei Akaboshi, Chengzhu Zhao, Mitsuaki Shibata, Sho Senda, Yayoi Toyooka, Hidetoshi Sakurai, Hidemi Kurihara, Makoto Ikeya
Summary: In this study, human induced pluripotent stem cells (iPSCs) were successfully induced into mesenchymal stem cells (MSCs) under xeno-free conditions. The xeno-free-induced MSCs (XF-iMSCs) were found to have regenerative potential in bone and skeletal muscle regeneration. They promoted the regeneration of surrounding host cells through the secretion of soluble factors, and the secretion of peroxidasin and IGF2 also contributed to myotube differentiation.
NPJ REGENERATIVE MEDICINE
(2022)
Review
Health Care Sciences & Services
Toshinori Ando, Kento Okamoto, Tomoaki Shintani, Souichi Yanamoto, Mutsumi Miyauchi, J. Silvio Gutkind, Mikihito Kajiya
Summary: Genetic alterations and dysregulation of signaling pathways are crucial for the development of cancer. Precision medicine allows for the selection of appropriate treatments based on genetic variations. Various genetic alterations in HNSCC disrupt the Hippo pathway and lead to hyperactivation of YAP/TAZ.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Tomoyuki Iwata, Eri Kaneda-Ikeda, Keita Takahashi, Katsuhiro Takeda, Takayoshi Nagahara, Mikihito Kajiya, Shinya Sasaki, Shu Ishida, Minami Yoshioka, Shinji Matsuda, Kazuhisa Ouhara, Tsuyoshi Fujita, Hidemi Kurihara, Noriyoshi Mizuno
Summary: This study aimed to investigate the regulatory mechanism of bone marrow-derived mesenchymal stem cell (BM-MSC) differentiation by humoral factors derived from human periodontal ligament (HPL) cells and human gingival fibroblasts (HGFs). The results showed that histone deacetylase (HDAC) expression and histone H3 acetylation were involved in the regulation of BM-MSC differentiation. In clinical applications, regulation of HDAC expression holds potential for effective periodontal regeneration, particularly bone regeneration.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Tatsuya Akitomo, Yuria Asao, Yuko Iwamoto, Satoru Kusaka, Momoko Usuda, Mariko Kametani, Toshinori Ando, Shinnichi Sakamoto, Chieko Mitsuhata, Mikihito Kajiya, Katsuyuki Kozai, Ryota Nomura
Summary: A 4-year-and-11-month-old boy had three supernumerary teeth, which required surgical treatment and long-term follow-up. The first supernumerary tooth was extracted at 5 years and 5 months, followed by two more at 7 years and 7 months and 8 years and 2 months. Malocclusion may be caused by the supernumerary teeth, and surgical exposure was performed to facilitate the eruption of the permanent teeth.
Article
Dentistry, Oral Surgery & Medicine
Fumitaka Obayashi, Koichi Koizumi, Nanako Ito, Nami Obayashi, Tomoaki Shintani, Mikihito Kajiya, Souichi Yanamoto
Summary: Palmoplantar pustulosis (PPP) is a stubborn skin disease characterized by small aseptic pustules on the palms and soles, which can worsen due to metal and dental focal infections. This study reports a case of PPP exacerbation after orthognathic surgery, attributing it to metal allergy from the plates or screws used.
Article
Cell & Tissue Engineering
Shin Morimoto, Mikihito Kajiya, Hiroki Yoshii, Mai Yoshino, Susumu Horikoshi, Souta Motoike, Tomoyuki Iwata, Kazuhisa Ouhara, Toshinori Ando, Tetsuya Yoshimoto, Tomoaki Shintani, Noriyoshi Mizuno
Summary: Three-dimensional clumps of MSCs/ECM complexes (C-MSCs) can be implanted without artificial scaffolds, and their cellular properties can be regulated in vitro. The study aimed to generate cartilaginous tissue covered with a mineralized bone collar-like structure from human C-MSCs by combining chondrogenic and osteogenic induction, showing potential for bone-regenerative cell therapy.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Health Care Sciences & Services
Hiromi Nishi, Susumu Horikoshi, Kouji Ohta, Tetsumi Yoshida, Noriyasu Fukushima, Kyoko Oshita, Syuichi Munenaga, Taro Edahiro, Hiroshi Ureshino, Hideo Shigeishi, Yukio Yoshioka, Masaru Konishi, Noriaki Ide, Yuma Ogawa, Rikou Marukawa, Tomoaki Shintani, Natumi Ino, Mikihito Kajiya, Naoya Kakimoto, Hiroki Ohge, Tatsuo Ichinohe, Hiroyuki Kawaguchi
Summary: LLLT has shown significant efficacy in preventing severe oral mucositis among hematologic cancer patients in Japan, leading to a lower incidence rate. This study fills the gap in clinical adoption of LLLT in Japan.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell & Tissue Engineering
Hiroki Yoshii, Mikihito Kajiya, Mai Yoshino, Shin Morimoto, Susumu Horikoshi, Misako Tari, Souta Motoike, Tomoyuki Iwata, Kazuhisa Ouhara, Toshinori Ando, Tetsuya Yoshimoto, Tomoaki Shintani, Noriyoshi Mizuno
Summary: This study investigates the role of mechanotransducer YAP/TAZ and their binding target transcriptional factor, TEAD, in the immunomodulatory capacities of human bone marrow-derived MSCs. The results show that disrupting the YAP/TAZ-TEAD axis can enhance the immunomodulatory properties of MSCs.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Endocrinology & Metabolism
Takumi Memida, Shinji Matsuda, Takashi Nakamoto, Kazuhisa Ouhara, Mikihito Kajiya, Shintaro Hirata, Eiji Sugiyama, Naoya Kakimoto, Noriyoshi Mizuno
Summary: Calcinosis is commonly seen in patients with systemic sclerosis, especially in the nasal spine area. In some cases, calcifications at the nasal spine can be replaced by cancellous bone-like tissue, which can be examined using CBCT or CT scans.