Article
Microbiology
V Chapot, L. Effenberg, J. Dohmen-Ruetten, J. Buer, J. Kehrmann
Summary: The study evaluated the performance of the Accelerate Pheno system for identification and AST from positive blood culture bottles inoculated with primary sterile specimens, showing that the system can accelerate pathogen identification and antimicrobial susceptibility testing, especially for hospitals without on-site microbiology laboratories. However, inclusion of nonblood specimens with a high likelihood of polymicrobial infections may lead to inferior performance.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Dorothy T. T. Sze, Candy C. Y. Lau, Tsz-Ming Chan, Edmond S. K. Ma, Bone S. F. Tang
Summary: This study evaluated the performance of different commercial methods for rapid identification and antimicrobial susceptibility testing (AST) in positive blood culture samples, and found that methods such as the Pheno system, BioFire FilmArray BCID panel, and VITEK 2 all demonstrated high accuracy and consistency. Factors such as turnaround time, range of antibiotic choices, and hands-on time should be considered when selecting appropriate rapid identification and AST methods for positive blood culture in different laboratory settings.
Article
Microbiology
Hazan Zengin Canalp, Banu Bayraktar
Summary: This study investigated the accuracy and feasibility of a rapid identification method for blood pathogens and found that the RID method significantly reduced the turnaround time compared to the SIRID method, making it a valuable tool for patient management.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
C. D. Garner, J. Brazelton de Cardenas, S. Suganda, R. T. Hayden
Summary: Bloodstream infections are a significant burden on healthcare systems, with rapid identification of pathogens crucial for patient outcomes. The GenMark Dx ePlex BCID panels show good performance in detecting bloodstream pathogens in high-risk populations, providing valuable diagnostic information for clinical care providers.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Daniel M. Webber, Meghan A. Wallace, Carey-Ann D. Burnham
Summary: By reducing the incubation time for disk diffusion testing from 24 hours to 6 hours, the turnaround time for antimicrobial susceptibility testing can be significantly improved without additional costs or equipment requirements.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Computer Science, Information Systems
Teresa Zayas-Caban, Tracy H. Okubo, Steven Posnack
Summary: Inefficient workflows in healthcare have negative impacts on various stakeholders, including patients, caregivers, clinicians, and staff. Advances in health information technology and computing present opportunities for automation, resulting in more efficient healthcare workflows. The Office of the National Coordinator for Health Information Technology (ONC) led a multidisciplinary effort to explore automation possibilities in healthcare through interviews, literature review, and workshops. This article discusses considerations, priorities, and strategies identified through this collaborative effort, emphasizing the role of informatics and research communities in advancing healthcare automation.
JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION
(2022)
Article
Microbiology
Thao T. Truong, Kanokporn Mongkolrattanothai, Irvin Ibarra Flores, Jennifer Dien Bard
Summary: Bloodstream infection is a serious medical emergency that requires timely administration of appropriate antibiotics. Conventional antimicrobial susceptibility testing methods can take several days, but the Accelerate Pheno Blood Culture panel offers a rapid testing option that can provide results within a few hours. This study evaluated the effectiveness of Pheno in pediatric patients with bloodstream infections and found that it accurately identified susceptibility and allowed for faster optimization of therapy.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Medical Laboratory Technology
David Chirio, Elisa Demonchy, Marion Le Marechal, Alice Gaudart, Romain Lotte, Michel Carles, Raymond Ruimy
Summary: 24/7 processing of blood cultures significantly reduces the turnaround time, time to effective antibiotic therapy, and time to antibiotic susceptibility testing results, which is crucial for improving the effectiveness of antibiotic treatment for patients.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Chemistry, Physical
Peter Sun, John A. Marohn
Summary: Simulation is crucial in designing and developing scientific experiments. Traditional procedural coding in simulations of complex experiments can be prone to errors, difficult to understand, and inflexible when incorporating changes. mmodel, a Python framework, offers a graph-theory approach to represent experiment steps and can modify its own code to accommodate updates like parameter looping. This framework aims to reduce duplication, enhance code clarity and testability, and expedite development time.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Infectious Diseases
Gabriele Bianco, Donatella Lombardo, Guido Ricciardelli, Matteo Boattini, Sara Comini, Rossana Cavallo, Cristina Costa, Simone Ambretti
Summary: The study evaluated the reliability and performance of the EUCAST RAST method, and found that it accurately determined microbial susceptibility to main antimicrobials. The method showed potential for antimicrobial de-escalation interventions.
Article
Infectious Diseases
Felix Roth, Nathan D. Leedahl, David D. Leedahl, Dubert M. Guerrero
Summary: The rapid identification of pathogens causing bloodstream infections is crucial in clinical microbiology laboratories. While most methods have reduced turnaround time and impact on clinical decisions, they do not provide complete antimicrobial susceptibility testing (AST) information. By employing rapid identification (ID) and AST with the Accelerate PhenoTest, the study observed a significant decrease in hospital stay, reduction in broad-spectrum antibiotic use, and potential cost savings.
Article
Microbiology
Yera A. Patel, Thomas J. Kirn, Melvin P. Weinstein, Priyanka Uprety
Summary: Bacteremia is a significant health issue, and rapid identification of pathogens is crucial for appropriate antimicrobial therapy. A study comparing Accelerate Pheno system with standard methods in a microbiology laboratory found reliable results for most antimicrobial agents for Enterobacterales, but limitations for some drugs. Additional research is needed to fully assess the performance of this system for various pathogens.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Jiahui Zhu, Ziwei Bao, Yan Xie, Jim Werngren, Yi Hu, Lina Davies Forsman, Judith Bruchfeld, Sven Hoffner
Summary: During the turnaround time (TAT) for drug susceptibility testing (DST) in pulmonary tuberculosis patients, a significant proportion developed drug resistance (DR) to first-line drugs, mainly due to exogenous reinfection. Rapid DST methods and improved infection control are urgently needed to address this issue.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Semjon Grinberg, Susanne Schubert, Kristina Hochauf-Stange, Alexander H. Dalpke, Marco Narvaez Encalada
Summary: In this study, a novel device for rapid identification and antimicrobial susceptibility testing from positive blood cultures was evaluated. The results showed that the device provided reliable and concordant results compared to the standard workflow, with significant time savings.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Keun Ju Kim, Seung Gyu Yun, Yunjung Cho, Myung-Hyun Nam, Young Jin Ko, Chang Kyu Lee
Summary: This study assessed the performance of an in-house method for rapid direct bacterial identification and antimicrobial susceptibility testing using positive blood culture broth. The method involved filtering the broth, centrifuging and washing the filtrate, and using mass spectrometry and automated broth microdilution for identification and testing. The in-house method had a correct identification rate of 94%, with rates of 91.4% and 97.3% for Gram-positive and Gram-negative isolates, respectively. The method also showed good agreement for antimicrobial susceptibility testing, with minor, major, and very major error rates of 3.8%, 3.4%, and 1.6%, respectively. This simple method can significantly shorten the turnaround time for identification and testing, potentially improving patient management.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)