Article
Infectious Diseases
Mariam Rima, Saoussen Oueslati, Laura Dabos, Dina Daaboul, Hassan Mallat, Elie Bou Raad, Marcel Achkar, Osman Mawlawi, Sandrine Bernabeu, Remy A. Bonnin, Delphine Girlich, Marwan Osman, Monzer Hamze, Thierry Naas
Summary: This study investigated the prevalence of carbapenem-resistant Gram-negative bacilli (CR-GNB) in three hospitals in northern Lebanon between 2015 and 2019. The findings highlighted a concerning situation of carbapenem resistance and emphasized the need for effective registration systems in healthcare centers to limit resistance spread.
Article
Infectious Diseases
M. Cristina Vazquez Guillamet, Christopher Damulira, Andrew Atkinson, Victoria J. Fraser, Scott Micek, Marin H. Kollef
Summary: The combination of aminoglycosides and β-lactams is more effective in reducing the incidence of infections caused by multidrug-resistant Gram-negative bacilli compared to β-lactams alone. This retrospective cohort study analyzed septic patients treated between 2010 and 2017, and found that the addition of aminoglycosides to β-lactams significantly decreased the risk of subsequent infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Kai Saito, Sayaka Mizuno, Ryuichi Nakano, Ayako Tanouchi, Tomoki Mizuno, Akiyo Nakano, Yuki Suzuki, Naoki Kakuta, Hisakazu Yano
Summary: Carbapenemase-producing Enterobacterales (CPE) are one of the most serious antimicrobial resistance threats to public health worldwide. Rapid detection of CPE infection is crucial for patient management. The NG-Test CARBA 5, an immunochromatographic assay, demonstrates high sensitivity and specificity for detecting the five most common carbapenemase producers.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Microbiology
Ines Rezzoug, Cecile Emeraud, Aimie Sauvadet, Garance Cotellon, Thierry Naas, Laurent Dortet
Summary: The MAST Carba PAcE test showed high specificity but lower sensitivity in detecting carbapenemase-producing Gram-negative bacilli. Interpretation difficulties, especially with OXA-48-like enzymes, were the main reason for the lower sensitivity of the test.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Review
Infectious Diseases
Zineb Cherak, Lotfi Loucif, Abdelhamid Moussi, Jean-Marc Rolain
Summary: Carbapenem resistance in Gram-negative bacteria is a major public health challenge, with transmission mainly occurring through water environments. Aquatic environments serve as a significant medium for antibiotic gene transfer, playing a crucial role in the spread of drug resistance in the environment and community.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Ting Shi, Liangyi Xie
Summary: This study investigated the antimicrobial resistance rates of gram-negative bacilli isolated from clinical settings. It was found that Acinetobacter baumannii and Klebsiella pneumoniae showed a rapid increase in resistance, while Escherichia coli had a relatively low and stable resistance rate. Gram-negative bacilli in the ICU department demonstrated higher resistance rates compared to the non-ICU department, with significant differences.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Phatsarawadee Hemwaranon, Arpasiri Srisrattakarn, Aroonlug Lulitanond, Patcharaporn Tippayawat, Ratree Tavichakorntrakool, Lumyai Wonglakorn, Jureerut Daduang, Aroonwadee Chanawong
Summary: Carbapenem-resistant Enterobacterales (CRE) with carbapenemases, especially OXA-48 group, are spreading rapidly and posing a global public health concern. The current phenotypic detection method for OXA-48-like carbapenemases is not optimal, while the PCR-based methods are time-consuming. In this study, a recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip assay was developed for the rapid detection of bla(OXA-48-like) genes in Enterobacterales. The assay showed excellent performance in sensitivity and specificity and had a detection limit 100 times lower than the PCR method.
Article
Dentistry, Oral Surgery & Medicine
Lais Christina Pontes Espindola, Renata Cristina Picao, Stella Maria Casas Novas Mancano, Renata Martins do Souto, Ana Paula Vieira Colombo
Summary: A high prevalence and diversity of Gram-negative bacilli, with low susceptibility to beta-lactams, were observed in the subgingival microbiota associated with periodontitis.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Infectious Diseases
Ayan Ali Ragueh, Mohamed Houmed Aboubaker, Sitani Idriss Mohamed, Jean-Marc Rolain, Seydina M. Diene
Summary: This study investigated the resistance rate to carbapenems among multidrug-resistant Gram-negative bacteria (MDR-GNB) in Djibouti and characterized their resistance mechanisms. The results showed that out of 256 isolates, 20 were resistant to carbapenems. Carbapenemase genes such as bla(OXA-48) and bla(NDM-5) were identified in the isolates.
Article
Microbiology
Carmen Antonia Sanches Ito, Larissa Bail, Lavinia Nery Villa Stangler Arend, Kleber Oliveira Silva, Simone Sebold Michelotto, Keite da Silva Nogueira, Felipe Francisco Tuon
Summary: The study evaluated the performance of a new MicroScan Neg multidrug-resistant MIC 1 (NMR1) panel for clinical carbapenem-nonsusceptible Gram-negative bacilli isolates, demonstrating high reliability in detecting ceftazidime-avibactam and ceftolozane-tazobactam. The panel is a viable option for clinical applications, especially for KPC-producing Enterobacterales and carbapenem-nonsusceptible P. aeruginosa.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Editorial Material
Immunology
Sean Wei Xiang Ong, Pei Yun Hon, Sharon Syn Hui Wee, Jonathan Wei Zhong Chia, Shehara Mendis, Ezlyn Izharuddin, Ray Junhao Lin, Po Ying Chia, Rees Chin Swee Sim, Mark I-Cheng Chen, Angela Chow, Joanne Yoong, David Chien Lye, Christine B. Teng, Paul Anantharajah Tambyah, Ritu Banerjee, Robin Patel, Partha Pratim De, Shawn Vasoo
Summary: The algorithm combining multiplex polymerase chain reaction with phenotypic detection of enzymes directly from positive blood culture bottles showed good concordance with routine cultures in patients with gram-negative bacteremia, suggesting it may improve time to optimal therapy in these patients.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Jose Raul Nieto-Saucedo, Luis Esau Lopez-Jacome, Rafael Franco-Cendejas, Claudia Adriana Colin-Castro, Melissa Hernandez-Duran, Luis Raul Rivera-Garay, Karina Senyase Zamarripa-Martinez, Juan Luis Mosqueda-Gomez
Summary: The prevalence of carbapenem-resistant Gram-negative bacilli (CR-GNB) and the frequency of carbapenemase-encoding genes were evaluated in a tertiary referral center in El Bajio, Mexico. Among 508 Gram-negative bacilli, 37 were carbapenem-resistant (7.3%), and only 15 (40.5%) of them carried carbapenemase-encoding genes.
Review
Immunology
Flora Cruz-Lopez, Adrian Martinez-Melendez, Rayo Morfin-Otero, Eduardo Rodriguez-Noriega, Hector J. Maldonado-Garza, Elvira Garza-Gonzalez
Summary: Infections caused by Gram-negative multi-drug resistant (MDR) bacterial species are difficult to treat due to widespread antibiotic resistance. New antibiotics and combinations with existing drugs have shown potential in treating these infections, but there are reports of resistance to these treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Vincent Plassard, Philippe Gisbert, Sophie A. Granier, Yves Millemann
Summary: The study aimed to test a surveillance protocol for detecting specific bacteria in dairy farms, with limitations including missing samples and lack of specificity in detection results.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Medical Laboratory Technology
Emily Sullivan, Maria D. Macias Jimenez, Nicholas M. Moore
Summary: This study evaluated the performance of a lateral flow assay (LFA) for the detection of carbapenemase production. The overall agreement of the LFA was 98.2% with results available in 15 minutes, compared to molecular detection which took 1 hour. These accurate and rapid diagnostic methods are crucial for antimicrobial stewardship programs.
LABORATORY MEDICINE
(2023)
Article
Immunology
Tom J. B. de Man, Anna Q. Yaffee, Wenming Zhu, Dhwani Batra, Efe Alyanak, Lori A. Rowe, Gillian McAllister, Heather Moulton-Meissner, Sandra Boyd, Andrea Flinchum, Rachel B. Slayton, Steven Hancock, Maroya Spalding Walters, Alison Laufer Halpin, James Kamile Rasheed, Judith Noble-Wang, Alexander J. Kallen, Brandi M. Limbago
Summary: This investigation identified the largest known outbreak of VIM-producing CRE in the United States, showcasing evidence of in-hospital plasmid transmission and environmental contamination.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Biotechnology & Applied Microbiology
Sean Wei Xiang Ong, Shawn Vasoo, Sapna P. Sadarangani, Lin Cui, Kalisvar Marimuthu, Poh Lian Lim, Jing Wen Kong, Judith Chui Ching Wong, Kim Yoong Puong, Kwai Peng Chan
Summary: Two adult patients presented with a typical erythematous rash consistent with rubella disease after MMR vaccination. Both patients had an uncomplicated clinical course, with one confirmed to have vaccine-associated rubella and the other having a positive pharyngeal swab for rubella virus PCR matching the vaccine strain. Further study is needed to determine the incidence, risk factors, and clinical characteristics of rubella vaccine-strain related disease.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2021)
Article
Public, Environmental & Occupational Health
Kristen Allison Perry-Dow, Tom J. B. de Man, Alison Laufer Halpin, Alicia M. Shams, Laura J. Rose, Judith A. Noble-Wang
Summary: The study aimed to characterize the microbial communities of disinfected healthcare surfaces using next generation sequencing technology. Results showed that disinfectant products may impact the healthcare environment microbiome, with rooms cleaned with bleach containing a higher proportion of gram-positive microbiota compared to those cleaned with a quaternary ammonium compound. Overall agreement between culture and sequence methods for detecting target pathogens ranged from 52%-88%.
AMERICAN JOURNAL OF INFECTION CONTROL
(2022)
Article
Infectious Diseases
Aubree J. Kelly, Melissa L. Ivey, Christopher A. Gulvik, Ben W. Humrighouse, John R. McQuiston
Summary: Rat bite fever caused by Streptobacillus moniliformis is difficult to diagnose, but a multiplex real-time PCR assay may aid in quick diagnosis by detecting the pathogen in clinical specimens.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Surgery
Jennifer A. Yonkus, Emma Whittle, Roberto Alva-Ruiz, Amro M. Abdelrahman, Susan E. Horsman, Gina A. Suh, Scott A. Cunningham, Heidi Nelson, Travis E. Grotz, Rory L. Smoot, Sean P. Cleary, David M. Nagorney, Michael L. Kendrick, Robin Patel, Mark J. Truty, Nicholas Chia
Summary: This study investigates the feasibility of nanopore sequencing in intraoperative bile samples and compares it with standard cultures. The results show that nanopore sequencing can provide microbial profiles more quickly, detect more bacterial species and resistance genotypes, and has a perfect negative predictive value. However, nanopore sequencing has higher associated costs compared to standard cultures.
Article
Medicine, General & Internal
Jay E. Gee, William A. Bower, Amber Kunkel, Julia Petras, Jenna Gettings, Maria Bye, Melanie Firestone, Mindy G. Elrod, Lindy Liu, David D. Blaney, Allison Zaldivar, Chelsea Raybern, Farah S. Ahmed, Heidi Honza, Shelley Stonecipher, Briana J. O'Sullivan, Ruth Lynfield, Melissa Hunter, Skyler Brennan, Jessica Pavlick, Julie Gabel, Cherie Drenzek, Rachel Geller, Crystal Lee, Jana M. Ritter, Sherif R. Zaki, Christopher A. Gulvik, W. Wyatt Wilson, Elizabeth Beshearse, Bart J. Currie, Jessica R. Webb, Zachary P. Weiner, Maria E. Negron, Alex R. Hoffmaster
Summary: Four cases of non-travel-associated melioidosis in the United States were linked to the use of a contaminated aromatherapy room spray.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Infectious Diseases
Heather P. McLaughlin, Christopher A. Gulvik, David Sue
Summary: Burkholderia pseudomallei causes a life-threatening disease and understanding its epidemiology and antibiotic resistance mechanisms can benefit public health and safety. In this study, the authors identified genes encoding penicillin binding proteins (PBPs) in B. pseudomallei and examined their utility for determining phylogeography and differentiating closely related species. They developed a dual-locus sequence typing (DLST) scheme based on single nucleotide polymorphisms (SNPs) and demonstrated the usefulness of PBPs for strain typing and predicting geographic origin.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Genetics & Heredity
Boas C. L. van der Putten, C. Mendes, Brooke M. Talbot, Jolinda De Korne-Elenbaas, Rafael Mamede, Pedro Vila-Cerqueira, Luis Pedro Coelho, Christopher A. Gulvik, Lee S. Katz
Summary: This research report presents seven recommendations for implementing software testing in microbial bioinformatics, based on the experience of a collaborative hackathon event. The report also provides a repository hosting examples and guidelines for testing.
MICROBIAL GENOMICS
(2022)
Article
Microbiology
Patrick Dawson, Johanna S. Salzer, Caroline A. Schrodt, Karl Feldmann, Cari B. Kolton, Jay E. Gee, Chung K. Marston, Christopher A. Gulvik, Mindy G. Elrod, Aaron Villarma, Rita M. Traxler, Maria E. Negron, Kate A. Hendricks, Heather Moulton-Meissner, Laura J. Rose, Paul Byers, Kathryn Taylor, Daphne Ware, Gary A. Balsamo, Theresa Sokol, Bret Barrett, Erica Payne, Saad Zaheer, Ga On Jung, Stephen Long, Ricardo Quijano, Lindsey LeBouf, Briana O'Sullivan, Erin Swaney, James M. Antonini, Marie A. de Perio, Zachary Weiner, William A. Bower, Alex R. Hoffmaster
Summary: Investigations of two cases of welder's anthrax found that the clinical isolates from one patient matched bacterial strains recovered from their work-related clothing and gear, as well as the worksite soil. All welder's anthrax cases should be investigated to determine the source of exposure.
Article
Microbiology
Laura M. Carroll, Chung K. Marston, Cari B. Kolton, Christopher A. Gulvik, Jay E. Gee, Zachary P. Weiner, Jasna Kovac
Summary: This study characterized three B. cereus s.l. isolates associated with two anthrax cases in welders in the United States in 2020. The genomes of these isolates belonged to different species, with two being closely related to B. tropicus and one being most similar to B. anthracis.
Review
Medical Laboratory Technology
Scott A. Cunningham, Jayawant N. Mandrekar, Gina Suh, Robin Patel
Summary: The study demonstrates that the described method provides good to excellent statistical reproducibility for assessing phage susceptibility of two commonly encountered bacterial species, with statistically excellent results for Escherichia coli and good/fair results for Staphylococcus aureus.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2022)
Correction
Public, Environmental & Occupational Health
Anastasia S. Lambrou, Philip Shirk, Molly K. Steele, Prabasaj Paul, Clinton R. Paden, Betsy Cadwell, Heather E. Reese, Yutaka Aoki, Norman Hassell, Jason Caravas, Nicholas A. Kovacs, Jonathan G. Gerhart, Han Jia Ng, Xiao-yu Zheng, Andrew Beck, Reina Chau, Roxana Cintron, Peter W. Cook, Christopher A. Gulvik, Dakota Howard, Yunho Jang, Kristen Knipe, Kristine A. Lacek, Kara A. Moser, Adrian C. Paskey, Benjamin L. Rambo-Martin, Roopa R. Nagilla, Adam C. Retchless, Matthew W. Schmerer, Sandra Seby, Samuel S. Shepard, Richard A. Stanton, Thomas J. Stark, Anna Uehara, Yvette Unoarumhi, Meghan L. Bentz, Alex Burgin, Mark Burroughs, Morgan L. Davis, Matthew W. Keller, Lisa M. Keong, Shoshona S. Le, Justin S. Lee, Joseph C. Madden, Sarah Nobles, D. Collins Owuor, Jasmine Padilla, Mili Sheth, Malania M. Wilson, Sarah Talarico, Jessica C. Chen, M. Steven Oberste, Dhwani Batra, Laura K. McMullan, Alison Laufer Halpin, Summer E. Galloway, Duncan R. MacCannell, Rebecca Kondor, John Barnes, Adam MacNeil, Benjamin J. Silk, Vivien G. Dugan, Heather M. Scobie, David E. Wentworth
MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT
(2022)
Letter
Immunology
Carolina Duarte, Franco Montufar, Jaime Moreno, Dora Sanchez, Jose Yesid Rodriguez, Alfredo G. Torres, Soraya Morales, Adriana Bautista, Monica G. Huertas, Julia N. Myers, Christopher A. Gulvik, Mindy G. Elrod, David D. Blaney, Jay E. Gee
Summary: An analysis of Burkholderia pseudomallei isolates recovered in Colombia during routine surveillance in 2016-2017 revealed high genetic diversity, indicating that the pathogen is well established and has spread across the region.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Microbiology
Christopher A. Gulvik, Dhwani Batra, Lori A. Rowe, Milli Sheth, Sarah Nobles, Justin S. Lee, John R. McQuiston, Brent A. Lasker
Summary: In this study, the complete and circular genome sequence of Gordonia sp. strain X0973, a Gram-positive, weakly acid-fast, aerobic actinomycete obtained from a human abscess, was assembled using Illumina MiSeq and PacBio reads. The genome is 3.75 Mbp in size and contains 3,601 predicted coding sequences.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Letter
Cardiac & Cardiovascular Systems
Bingwen Eugene Fan, Thirugnanam Umapathi, Karen Chua, Yew Woon Chia, Shiun Woei Wong, Glenn Wei Leong Tan, Sadhana Chandrasekar, Ying Hui Lum, Shawn Vasoo, Rinkoo Dalan
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2021)
