Article
Cell Biology
So Jung Park, Rebecca A. Frake, David C. Rubinsztein
Summary: Impaired autophagosome formation and reduced flux through the macroautophagy/autophagy pathway occur outside the brain as part of normal aging. Our study found that SORBS3 gene expression is significantly increased in the brains of older mice and humans. SORBS3 negatively regulates autophagy in several cell lines and its depletion leads to increased autophagosome formation. The upregulation of YAP1-WWTR1/TAZ target genes, including myosin- and actin-related genes, contributes to this process. Moreover, we observed downregulation of these target genes in older mouse and human brains. These findings suggest that increased SORBS3 expression contributes to autophagic decline in normal brain aging.
Article
Environmental Sciences
Keegan Lopez, Alexandra Camacho, Quiteria Jacquez, Mary Kay Amistadi, Sebastian Medina, Katherine Zychowski
Summary: This study assessed the mechanisms and systemic health impacts of inhaled particulate matter (PM) in a rodent model. The results showed that CCL-2 was upregulated in lung tissue and downregulated in the brain following PM exposure. Additionally, exposure to mine-PM resulted in cerebrovascular barrier integrity deficits and increased GFAP staining, dependent on exosome inhibition. The open-field test revealed a higher stress and anxiety response in the mine-PM exposure group, which was mitigated with GW4869 intervention. Exosome lipidomics revealed substantial alterations in lipid profiles. Overall, this study suggests that lung-derived, circulating exosomes play a role in driving systemic, proinflammatory effects of inhaled PM.
Article
Multidisciplinary Sciences
Xiang-Zhi Fang, Min Li, Ya-Xin Wang, Pei Zhang, Miao-Miao Sun, Jia-Xin Xu, Yi-Yi Yang, Ya-Jun He, Yuan Yu, Rui-Ting Li, Ting Zhou, Le-Hao Reng, De-Yi Sun, Hua-Qing Shu, Shi-Ying Yuan, Ji-Qian Xu, You Shang
Summary: This study demonstrated the key role of Piezo1 in mechanical ventilation-exacerbated ARDS-associated pulmonary fibrosis. Inhibiting Piezo1 activity could attenuate ATP release and EMT process in lung epithelial cells, thus improving pulmonary fibrosis.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Critical Care Medicine
Bingqiang Wen, Enhong Li, Vladimir Ustiyan, Guolun Wang, Minzhe Guo, Cheng-Lun Na, Gregory T. Kalin, Veronica Galvan, Yan Xu, Timothy E. Weaver, Tanya Kalin, Jeffrey A. Whitsett, Vladimir V. Kalinichenko
Summary: The study demonstrated the capability of ESCs to generate lung tissues in Nkx2-1(-/-) mouse embryos with lung agenesis through blastocyst complementation, indicating highly differentiated ESC-derived cells from multiple respiratory cell lineages that were indistinguishable from endogenous cells.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Cell Biology
Bowen Zhu, Megan Finch-Edmondson, Kim Whye Leong, Xiaoqian Zhang, V Mitheera, Quy Xiao Xuan Lin, Yaelim Lee, Wei Ting Ng, Huili Guo, Yue Wan, Marius Sudol, Ramanuj DasGupta
Summary: Long non-coding RNAs (lncRNAs) play critical roles in development and carcinogenesis, while the Hippo-YAP/TAZ signaling pathway is essential for organ size control and often dysregulated in cancers. SFTA1P was identified as a novel transcriptional target and positive feedback regulator of YAP/TAZ signaling, with knockdown inhibiting proliferation and inducing cell death in NSCLC cell lines. Mechanistically, SFTA1P knockdown decreased TAZ protein abundance, affecting the expression of YAP/TAZ transcriptional targets, suggesting its potential role in lncRNA-based therapies against YAP/TAZ-driven cancers.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Yang Liu, Yuewen Luo, Shumei Yan, Yi-Fan Lian, Shiyu Wu, Miao Xu, Lin Feng, Xu Zhang, Rong Li, Xiantao Zhang, Qi-Sheng Feng, Yi-Xin Zeng, Hui Zhang
Summary: In this study, the role of KLHDC3 in non-small cell lung cancer (NSCLC) was investigated. Higher expression of KLHDC3 was associated with worse overall survival in NSCLC patients. KLHDC3 deficiency affected the proliferation of NSCLC cell lines and its interaction with CUL2 E3 ligase and tumor suppressor p14ARF. Gefitinib-resistant NSCLC cell lines displayed higher KLHDC3 protein levels. Furthermore, KLHDC3 shortage increased the sensitivity of lung cancer cells to EGFR-targeted drugs.
Article
Endocrinology & Metabolism
Laura Croce, Elisabetta Zampogna, Francesca Coperchini, Pietro Costa, Patrizia Pignatti, Dina Visca, Antonio Spanevello, Mario Rotondi
Summary: The aim of this study was to evaluate thyroid function parameters among COVID-19 patients and observe their changes during rehabilitation. The results showed that FT3 values increased and functional capabilities improved during respiratory rehabilitation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Medicine, Research & Experimental
Bianca M. . Vamesu, Teodora Nicola, Rui Li, Snehashis Hazra, Sadis Matalon, Naftali Kaminski, Namasivayam Ambalavanan, Jegen Kandasamy
Summary: Mitochondrial dysfunction at birth is associated with the development of bronchopulmonary dysplasia (BPD) in extremely low-birth weight (ELBW) infants. Nebulized thyroid hormone (TH) has shown potential to improve mitochondrial function and reduce pulmonary fibrosis in adult animals. This study investigated the effects of intranasal T3 on neonatal lung injury and mitochondrial dysfunction in newborn mice, as well as its impact on lung fibroblasts and mesenchymal stem cells from ELBW infants. The results demonstrated that inhaled T3 attenuated hyperoxia-induced lung injury and improved mitochondrial function in mice, suggesting that TH supplementation might be a valuable therapeutic strategy for BPD.
Editorial Material
Cardiac & Cardiovascular Systems
David J. Hall, Jefree J. Schulte, Erik E. Lewis, Swaroop R. Bommareddi, Charles T. Rohrer, Samir Sultan, James D. Maloney, Malcolm M. DeCamp, Daniel P. McCarthy
Summary: This article reports a case of lung transplantation for post-COVID-19 pulmonary fibrosis. The patient recovered rapidly after the surgery and has done well in follow-up.
ANNALS OF THORACIC SURGERY
(2022)
Article
Oncology
Bowen Zhu, V Mitheera, Megan Finch-Edmondson, Yaelim Lee, Yue Wan, Marius Sudol, Ramanuj DasGupta
Summary: The study identified miR-582-5p as a post-transcriptional regulator of the Hippo-YAP/TAZ signaling pathway in NSCLC cells, inhibiting YAP/TAZ signaling by targeting NCKAP1 and PIP5K1C. The findings highlight the potential therapeutic value of restoring miR-582-5p expression in the treatment of lung cancer driven by YAP/TAZ.
Article
Pathology
Kei Sano, Takuo Hayashi, Yoshiyuki Suehara, Masaki Hosoya, Kazuya Takamochi, Shinji Kohsaka, Satsuki Kishikawa, Monami Kishi, Satomi Saito, Fumiyuki Takahashi, Kazuo Kaneko, Kenji Suzuki, Takashi Yao, Muneaki Ishijima, Tsuyoshi Saito
Summary: In lung adenocarcinoma, different isoforms of NKX2-1/TTF-1 are encoded by multiple transcription start sites, with some isoforms associated with prognosis. The expression of certain isoforms, particularly those without exon 1, may define subsets of aggressive LAD in elder patients, indicating potential clinical implications.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2021)
Article
Physiology
Yi Zhang, Guoying Yu, Naftali Kaminski, Patty J. Lee
Summary: The study reveals that thyroid hormone can reduce lung injury and inflammation in a hyperoxic environment, promoting mitochondrial survival. PINK1(-/-) mice are more susceptible to hyperoxia, but pretreatment with T3 can improve their mitochondrial function. Therefore, PINK1 plays a crucial role in mediating the protective effects of thyroid hormone in acute lung injury.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Respiratory System
Tadafumi Yokoyama, Bernadette R. Gochuico
Summary: Pulmonary fibrosis is a progressive interstitial lung disease with poor prognosis. Genetic studies of Hermansky-Pudlak syndrome (HPS) have provided insights into the pathogenesis of the disease, revealing a high prevalence of pulmonary fibrosis in three genetic types of HPS. HPS pulmonary fibrosis shares some clinical features with idiopathic pulmonary fibrosis but differs in age of onset and CT findings.
EUROPEAN RESPIRATORY REVIEW
(2021)
Article
Oncology
Janice Kim, Diana Bradford, Erin Larkins, Lee H. Pai-Scherf, Somak Chatterjee, Pallavi S. Mishra-Kalyani, Emily Wearne, Whitney S. Helms, Amal Ayyoub, Youwei Bi, Jielin Sun, Rosane Charlab, Jiang Liu, Hong Zhao, Dun Liang, Soma Ghosh, Reena Philip, Richard Pazdur, Marc R. Theoret, Julia A. Beaver, Harpreet Singh
Summary: The FDA granted accelerated approval for pralsetinib for non-small cell lung cancer and thyroid cancer, based on substantial overall response rates and durable responses in patients with RET-altered tumors. However, the product label includes warnings and precautions for various adverse events and risks.
CLINICAL CANCER RESEARCH
(2021)
Article
Hematology
Matt S. Zinter, Caroline A. Lindemans, Birgitta A. Versluys, Madeline Y. Mayday, Sara Sunshine, Gustavo Reyes, Marina Sirota, Anil Sapru, Michael A. Matthay, Sandhya Kharbanda, Christopher C. Dvorak, Jaap J. Boelens, Joseph L. DeRisi
Summary: This study analyzed biospecimens from 181 pediatric HCT candidates to investigate the association between pre-HCT pulmonary metatranscriptomes and post-HCT lung injury. The results showed that certain gene expression patterns and microbiome compositions in the lungs prior to transplantation were linked to an increased risk of developing lung injury after the procedure. Data from this study suggest that assessment of pre-HCT BAL fluid could potentially help identify high-risk pediatric HCT candidates who may benefit from targeted interventions.
Article
Genetics & Heredity
Arrate Pereda, Intza Garin, Guiomar Perez de Nanclares
BMC MEDICAL GENETICS
(2018)
Article
Genetics & Heredity
Maria Elena Rodriguez-Garcia, Francisco Javier Cotrina-Vinagre, Jaime Cruz-Rojo, Lucia Garzon-Lorenzo, Patricia Carnicero-Rodriguez, Jaime Sanchez-Del Pozo, Francisco Martinez-Azorin
AMERICAN JOURNAL OF MEDICAL GENETICS PART A
(2018)
Article
Genetics & Heredity
Carmen Rodriguez-Jimenez, Olga Pernia, Jose Mostaza, Carlos Rodriguez-Antolin, Juan de Dios Garcia-Diaz, Concepcion Alonso-Cerezo, Iluminada Garcia-Polo, Agustin Blanco, Carlos Lahoz, Francisco Arrieta, Luis Beltran, Aranzazu Diaz de Bustamante, Lucia Garzon-Lorenzo, Luis Antonio Alvarez-Sala, Angel Asenjo, Inmaculada Ibanez de Caceres, Sonia Rodriguez-Novoa
Review
Oncology
Miguel A. Zaballos, Adrian Acuna-Ruiz, Marta Morante, Piero Crespo, Pilar Santisteban
ENDOCRINE-RELATED CANCER
(2019)
Article
Multidisciplinary Sciences
Julia Ramirez-Moya, Leon Wert-Lamas, Adrian Acuna-Ruiz, Miguel A. Zabellos, Pilar Santisteban
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Endocrinology & Metabolism
Lucia Sentchordi-Montane, Sara Benito-Sanz, Miriam Aza-Carmona, Arrate Pereda, Manuel Parron-Pajares, Carolina de la Torre, Gabriela A. Vasques, Mariana F. A. Funari, Andre M. Travessa, Patricia Dias, Larisa Suarez-Ortega, Jesus Gonzalez-Buitrago, Nancy Elizabeth Portillo-Najera, Isabel Llano-Rivas, Maria Martin-Frias, Joaquin Ramirez-Fernandez, Jaime Sanchez del Pozo, Lucia Garzon-Lorenzo, Gabriel A. Martos-Moreno, Cristina Alfaro-Iznaola, Ines Mulero-Collantes, Pablo Ruiz-Ocana, Paula Casano-Sancho, Ana Portela, Lorea Ruiz-Perez, Angela del Pozo, Elena Vallespin, Mario Solis, Antonio M. Lerario, Isabel Gonzalez-Casado, Purificacion Ros-Perez, Guiomar Perez de Nanclares, Alexander A. L. Jorge, Karen E. Heath
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Letter
Oncology
Raquel Olivas-Mazon, Maria Martin-Cazana, Patricia Perez-Mohand, Lucia Garzon-Lorenzo, Mar Espino-Hernandez, Maria Baro-Fernandez, Vanesa Perez-Alonso
PEDIATRIC BLOOD & CANCER
(2020)
Article
Biochemistry & Molecular Biology
Julia Ramirez-Moya, Christos Miliotis, Allison R. Baker, Richard Gregory, Frank J. Slack, Pilar Santisteban
Summary: ADAR1 deregulation leads to increased A-to-I editing in thyroid tumors, with CDK13 being identified as an oncogene that is significantly over-edited in tumor samples. This editing event promotes cancer cell hallmarks and increases the nucleolar abundance of the protein, potentially explaining the global splicing changes induced by ADAR1 deregulation.
Article
Genetics & Heredity
Juan F. Quesada-Espinosa, Lucia Garzon-Lorenzo, Jose M. Lezana-Rosales, Maria J. Gomez-Rodriguez, Maria T. Sanchez-Calvin, Carmen Palma-Milla, Irene Gomez-Manjon, Irene Hidalgo-Mayoral, Ruben Perez de la Fuente, Ana Arteche-Lopez, Maria I. Alvarez-Mora, Ana Camacho-Salas, Jaime Cruz-Rojo, Irene Lazaro-Rodriguez, Montserrat Morales-Conejo, Noemi Nunez-Enamorado, Ana Bustamante-Aragones, Rogelio Simon de las Heras, Maria A. Gomez-Cano, Patricia Ramos-Gomez, Ollalla Sierra-Tomillo, Alexandra Juarez-Rufian, Jesus Gallego-Merlo, Laura Rausell-Sanchez, Marta Moreno-Garcia, Jaime Sanchez del Pozo
Summary: Allan-Herndon-Dudley syndrome is an X-linked recessive disorder caused by pathogenic variants in the SLC16A2 gene, resulting in impaired thyroid metabolism and abnormal transport of thyroid hormones to the brain. A female patient with a complete phenotype of Allan-Herndon-Dudley was found to have a de novo 543-kb deletion on the X chromosome that includes the SLC16A2 gene and JPX and FTX genes, known to play a role in X chromosome inactivation. Studies showed preferential expression of the X chromosome with the deletion of JPX and FTX in the patient.
Article
Oncology
Allison R. Baker, Christos Miliotis, Julia Ramirez-Moya, Talia Marc, Ioannis S. Vlachos, Pilar Santisteban, Frank J. Slack
Summary: ADAR1 plays a critical role in triple-negative breast cancer, and its loss leads to reduced cell growth, invasion, and mammosphere formation. ADAR1 regulates both coding and noncoding targets by altering gene expression level, A-to-I editing, and splicing. The study also reveals that several tumor suppressor miRNAs are upregulated upon ADAR1 loss, suppressing cell-cycle progression and invasion.
MOLECULAR CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Miguel A. Zaballos, Adrian Acuna-Ruiz, Marta Morante, Garcilaso Riesco-Eizaguirre, Piero Crespo, Pilar Santisteban
Summary: The study demonstrates that BRAF- and RAS-mutant thyroid cells respond differently to DEL-22379, which cannot be explained by the previously described mechanism of action of the inhibitor. Nonetheless, DEL-22379 exhibits significant anti-tumor effects against BRAF-mutant cells in vivo, while the anti-tumor effects are mild for RAS-mutant cells.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Endocrinology & Metabolism
Marta Garcia, Ana C. Barreda-Bonis, Paula Jimenez, Ignacio Rabanal, Arancha Ortiz, Elena Vallespin, Angela del Pozo, Juan Martinez-San Milian, Isabel Gonzalez-Casado, Jose C. Moreno
JOURNAL OF THE ENDOCRINE SOCIETY
(2019)