Review
Pharmacology & Pharmacy
Bruno Charlier, Albino Coglianese, Federica De Rosa, Ugo de Grazia, Francesca Felicia Operto, Giangennaro Coppola, Amelia Filippelli, Fabrizio Dal Piaz, Viviana Izzo
Summary: Epilepsy is a complex neurological disorder with various syndromes and treatments, including the use of antiseizure medications (ASMs) that act on synaptic levels. Monitoring ASMs levels in biological fluids is crucial to minimize risks, and measuring the free drug fraction is increasingly important for better correlation with pharmacological and toxicological effects. Validated methodologies exist for separating and quantifying different drug forms, but further validation studies are needed to connect pharmacokinetic monitoring with clinical outcomes in real-world settings.
Article
Biochemical Research Methods
Hao Zhou, Min Zhang, Ling Sun, Wenfang Li, Qian Liu
Summary: A rapid and sensitive LC-MS/MS method was developed for simultaneous determination of cilostazol and ambroxol in rat plasma. Validation results showed that the method met the requirements for the determination of biological samples according to FDA guidelines.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Infectious Diseases
Maria Aquino, Maria Tinoco, Joana Bicker, Amilcar Falcao, Marilia Rocha, Ana Fortuna
Summary: Amikacin is the preferred antibiotic for treating Gram-negative infections in neutropenic oncology patients. However, there is a lack of population pharmacokinetic studies on amikacin in this patient population. This large-scale retrospective study investigated the effects of tumor diseases, chemotherapy, lag time, age, and renal function on amikacin pharmacokinetics in neutropenic cancer patients. The results showed that oncology patients received higher daily doses of amikacin and had increased drug clearance. Chemotherapy influenced amikacin pharmacokinetics and drug clearance decreased with longer lag time. The impact of aging on amikacin doses was stronger than chemotherapy. This research suggests that cancer patients may require higher initial doses of amikacin, especially within 30 days of starting chemotherapy.
Article
Endocrinology & Metabolism
Rossella Mazzilli, Martina Curto, Donatella De Bernardini, Soraya Olana, Matilde Capi, Gerardo Salerno, Fabiola Cipolla, Virginia Zamponi, Daniele Santi, Fernando Mazzilli, Maurizio Simmaco, Luana Lionetto
Summary: This observational study aimed to develop a new LC-MS/MS method for TDM of psychotropic drugs in seminal fluid. The results showed that seminal levels of certain psychotropic drugs were detectable and correlated with alterations in sperm concentration and motility, especially in patients treated with antipsychotics. Further studies are needed to validate these findings and explore the potential impact of semen TDM in managing male fertility issues related to psychotropic medications.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Food Science & Technology
Sara Morello, Sabina Pederiva, Rosa Avolio, Giuseppina Amato, Simona Zoppi, Alessia Di Blasio, Maria Cesarina Abete, Cristina Casalone, Rosanna Desiato, Giuseppe Ru, Daniela Marchis
Summary: The EU has lifted restrictions on using non-ruminant processed animal proteins in aquafeed, with potential future permission for poultry and pig feed, while also evaluating potential hazards related to antibiotic residues. Monitoring studies have detected tetracyclines in processed animal proteins, raising concerns about exposure to antibiotic residues.
Article
Pharmacology & Pharmacy
Liang Liu, Liu Zhang, Xiangyi Zheng, Xing Liu, Wei Liu, Jianhua Wu
Summary: In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform was developed for therapeutic drug monitoring (TDM) of 14 antibacterial and antifungal agents. The method is simple, sensitive, and only requires 100 μL of serum. After validation, it has been successfully used for TDM in routine practice.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Martina Zanchetta, Valentina Iacuzzi, Bianca Posocco, Giorgia Bortolin, Ariana Soledad Poetto, Marco Orleni, Giovanni Canil, Michela Guardascione, Luisa Foltran, Valentina Fanotto, Fabio Puglisi, Sara Gagno, Giuseppe Toffoli
Summary: A sensitive, rapid, and cost-effective LC-MS/MS method was developed and validated for the quantification of LENVA in human plasma in this study. The method met EMA and FDA guidelines with good linearity, precision, and accuracy, and was successfully applied to patient plasma samples.
Article
Biochemical Research Methods
Agnija Kivrane, Solveiga Grinberga, Eduards Sevostjanovs, Viktorija Igumnova, Ilva Pole, Anda Viksna, Dace Bandere, Alvils Krams, Andra Cirule, Osvalds Pugovics, Renate Ranka
Summary: The developed LC-MS/MS method allows simultaneous quantification of first-line antituberculosis drugs and their metabolites in human plasma. The simple single-step protein precipitation with methanol was chosen as the most convenient sample pretreatment method, achieving good accuracy and precision in analyte quantification.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Applied
Peizhen Li, Rongmao Lu, Jie Zhu, Qingqing Ke, Dingnan Wang, Yang Wang, Chen Zhang, Xinyue Lu, Miaolong Zhao, Zhang Wang, Lingling Pan, Meiling Zhang, Jun Ying, Shiyan Li
Summary: A sensitive and reliable ultra-performance hydrophilic interaction chromatography-tandem mass spectrometry method was developed to determine paralytic shellfish toxins (PSTs) and tetrodotoxin (TTX) in marine shellfish. The method involved the use of a HILIC-Z column for chromatographic separation and a porous carbon solid-phase extraction for sample clean-up. The calibration curves for PSTs and TTX were linear, with good sensitivity and low limits of detection and quantitation. The method was successfully applied to determine PSTs and TTX in three batches of Nassarius, showing excellent accuracy.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Chemistry, Analytical
Xuefei Zhong, Shruti Nayak, Lili Guo, Shivkumar Raidas, Yunlong Zhao, Rachel Weiss, Matthew Andisik, Chinnasamy Elango, Giane Sumner, Susan C. Irvin, Michael A. Partridge, Hong Yan, Sook E. Yen, Haibo Qiu, Yuan Mao, Albert Torri, Ning Li
Summary: REGEN-COV, a cocktail of two human monoclonal antibodies, has shown promising results in reducing SARS-CoV-2 viral load in COVID-19 patients. A liquid chromatography-mass spectrometry method was developed for the determination of REGEN-COV concentrations in serum samples from patients participating in clinical trials. The concentrations measured by this method were comparable to those measured by a validated immunoassay.
ANALYTICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Congrong Tang, Xiaohui Niu, Lu Shi, Huidan Zhu, Guanyang Lin, Ren-ai Xu
Summary: In this study, a sensitive UPLC-MS/MS method was developed to detect fedratinib concentrations in rat plasma and investigate the impact of antifungal drugs on fedratinib metabolism. Fluconazole was found to have the strongest inhibitory effect on fedratinib metabolism in rats.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medical Laboratory Technology
Susanne Weber, Sara Tombelli, Ambra Giannetti, Cosimo Trono, Mark O'Connell, Ming Wen, Ana B. Descalzo, Heike Bittersohl, Andreas Bietenbeck, Pierre Marquet, Lutz Renders, Guillermo Orellana, Francesco Baldini, Peter B. Luppa
Summary: Therapeutic drug monitoring (TDM) is crucial in personalized medicine, helping clinicians optimize therapy through understanding pharmacokinetics and pharmacodynamics. This study used LC-MS/MS to analyze drug concentrations in kidney transplant patients, showing that microdialysis-supported immunosensor allows real-time analysis and tailor-made dosing.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Infectious Diseases
Qian Liu, Lanyu Liu, Yu Yuan, Feifan Xie
Summary: This study developed a dried blood spots (DBS) method for pharmacokinetic studies in pediatric patients. The method used the Capitainer(R) qDBS device to collect a 10 mu L volume of blood on a paper card, and quantified the concentrations of eight antibiotics using UHPLC-MS/MS. The DBS method was shown to be simple, robust, and suitable for children with limited blood sampling.
Article
Chemistry, Applied
Cristina Santos-Santorum Suarez, Pascal Sanders, Agnes Perrin-Guyomard, Dominique Hurtaud-Pessel, Michel Laurentie, Alexis Viel, Jean-Francois Taillandier, Marie-Pierre Lagree, Murielle Gaugain
Summary: Cross-contamination between medicated and non-medicated feed can lead to the presence of low concentrations of antibiotics in animal feed, posing potential risks to consumers and the emergence of resistant bacteria.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Chemistry, Medicinal
Changhee Ha, Hyun-Seung Lee, Eun Yeon Joo, Young-Min Shon, Seung Bong Hong, Dae-Won Seo, Soo-Youn Lee
Summary: The study investigated the therapeutic drug monitoring of Levetiracetam in Korean epilepsy patients, revealing a large inter-individual difference in its pharmacokinetics and its susceptibility to EIAEDs. There was a strong correlation between weight-adjusted Levetiracetam dosage and concentrations.
Review
Pharmacology & Pharmacy
Femke de Velde, Johan W. Mouton, Brenda C. M. de Winter, Teun van Gelder, Birgit C. P. Koch
PHARMACOLOGICAL RESEARCH
(2018)
Article
Infectious Diseases
A. Abdulla, T. M. J. Ewoldt, N. G. M. Hunfeld, A. E. Muller, W. J. R. Rietdijk, S. Polinder, T. van Gelder, H. Endeman, B. C. P. Koch
BMC INFECTIOUS DISEASES
(2020)
Article
Pharmacology & Pharmacy
Marith I. Francke, Dennis A. Hesselink, Yi Li, Birgit C. P. Koch, Lucia E. A. de Wit, Ron H. N. van Schaik, Lin Yang, Carla C. Baan, Teun van Gelder, Brenda C. M. de Winter
Summary: The study found poor correlation between [Tac](blood) and [Tac](cells), with age, albumin, and haematocrit correlating with the [Tac](cells)/[Tac](blood) ratio, while genetic variations in ABCB1, CYP3A4, and CYP3A5 did not. Neither [Tac](blood) nor [Tac](cells) were correlated with clinical outcomes.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Z. Demir, S. Bahmany, C. Bethlehem, J. van Oldenrijk, P. K. Bos, B. C. P. Koch
Summary: Total hip and knee arthroplasty have successful outcomes, but a small percentage of patients may suffer from postoperative periprosthetic joint infections. Standard treatment involves staged arthroplasty exchange with antibiotic therapy playing a crucial role. A validated UPC2-MS/MS method for quantification of antibiotics in synovial fluid was described, providing important insights for future PK/PD studies in determining exposure of antibiotics at the site of PJIs.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Clinical Neurology
Heidi M. Lammers-van der Holst, Gert Jan Lammers, Gijsbertus T. J. van Der Horst, Ines Chaves, Rory D. de Vries, Corine H. GeurtsvanKessel, Birgit Koch, Hugo M. van Der Kuy
Summary: This study aims to investigate the relationship between sleep, shift work, and COVID-19 vaccine immune response, hypothesizing that recent night shifts and lack of sleep among shift workers may negatively impact vaccine efficacy. With 50 shift workers and 50 dayworkers recruited, immune responsiveness will be assessed through blood tests and sleep monitoring, providing insights for potential interventions to enhance vaccination immunity.
JOURNAL OF SLEEP RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Eveline E. Roelofsen, Alan Abdulla, Anouk E. Muller, Henrik Endeman, Diederik Gommers, Annemieke Dijkstra, Nicole G. M. Hunfeld, Brenda C. M. de Winter, Birgit C. P. Koch
Summary: This study describes the pharmacokinetics of cefotaxime in critically ill patients and identifies factors such as estimated glomerular filtration rate and albumin concentration that influence clearance. For Enterobacterales, a dose of 1 g every 8 hours or continuous infusion of 4 g over 24 hours is sufficient. For Staphylococcus aureus, a continuous infusion of 6 g over 24 hours is recommended if estimated glomerular filtration rate and albumin concentration exceed certain thresholds.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Michelle Broekhuizen, Rene de Vries, Marja A. W. Smits, Willem A. Dik, Sam Schoenmakers, Birgit C. P. Koch, Daphne Merkus, Irwin K. M. Reiss, A. H. Jan Danser, Sinno H. P. Simons, Emilie Hitzerd
Summary: This study investigated the placental transfer, vascular effects, and anti-inflammatory actions of pentoxifylline in healthy and pre-eclamptic human placentas. The results showed that while pentoxifylline had beneficial effects on vasodilation and inflammation in healthy placentas, these effects were not retained in pre-eclamptic placentas. This limits the application of pentoxifylline in the treatment of pre-eclampsia, but it may still have value for other placenta-related disorders.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tim M. J. Ewoldt, Alan Abdulla, Nicole Hunfeld, Letao Li, Tim J. L. Smeets, Diederik Gommers, Birgit C. P. Koch, Henrik Endeman
Summary: Hepatic drug metabolism is crucial in improving drug dosing strategies in sepsis. This review examines the impact of sepsis on hepatic drug metabolism in humans, discussing clinical trials, pathophysiological background information, and example drug groups. Limited research suggests that hepatic drug metabolism may be decreased for certain drugs in sepsis. More studies are needed to understand the pharmacokinetic behavior of hepatic-metabolized drugs in sepsis, considering potential confounders such as inflammatory biomarkers and hemodynamic changes.
EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Birgit C. P. Koch, Qiaolin Zhao, Maartje Oosterhoff, Jakob van Oldenrijk, Alan Abdulla, Brenda C. M. de Winter, Koen Bos, Anouk E. Muller
Summary: This review aims to investigate the relationship between the target site concentration of antibiotics and the minimal inhibitory concentration in bone and joint infections (BJI). The literature on this topic is limited and there are variations in reported antibiotic concentrations. Some antibiotics, such as ciprofloxacin, cefazolin, cefuroxime, vancomycin, and linezolid, appear to have adequate exposure, while the results for other antibiotics are inconclusive. More research is needed in pharmacokinetic/pharmacodynamic modeling in BJI.
EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY
(2022)
Article
Infectious Diseases
Andre Wieringa, Peter G. J. ter Horst, Gertjan H. J. Wagenvoort, Annemieke Dijkstra, Alan Abdulla, Jasper J. Haringman, Birgit C. P. Koch
Summary: Limited data on the antimicrobial target attainment and pharmacokinetics of cefotaxime in critically ill patients undergoing continuous kidney replacement therapy (CKRT) have been studied. This study found that cefotaxime dosage of 1000 mg three to four times daily achieved adequate plasma concentrations in CKRT patients with anuria or oliguria, with the higher dosage recommended for patients with partially preserved renal function.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Critical Care Medicine
T. J. L. Smeets, D. van de Velde, B. C. P. Koch, H. Endeman, N. G. M. Hunfeld
Summary: This study validated the use of lithium heparin syringes for sampling vancomycin and compared it with conventional methods. Results showed no significant difference in vancomycin concentrations between the two methods, and using lithium heparin syringes for sampling can potentially save materials and reduce the number of blood draws.
CRITICAL CARE RESEARCH AND PRACTICE
(2022)
Article
Infectious Diseases
Tim M. J. Ewoldt, Soma Bahmany, Alan Abdulla, Anouk E. Muller, Henrik Endeman, Birgit C. P. Koch
Summary: Standard antibiotic dosing is not suitable for critically ill patients due to altered pharmacokinetics. Knowledge of protein binding is important for optimizing antibiotic exposure. This study found significant differences in unbound fractions between trough and peak samples, not explained by concentration differences. Existing models have good sensitivity for high concentrations but low specificity for subtherapeutic concentrations.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Critical Care Medicine
Alan Abdulla, Annemieke Dijkstra, Nicole G. M. Hunfeld, Henrik Endeman, Soma Bahmany, Tim M. J. Ewoldt, Anouk E. Muller, Teun van Gelder, Diederik Gommers, Birgit C. P. Koch
Article
Pharmacology & Pharmacy
Linda G. Franken, Brenda C. M. de Winter, Anniek D. Masman, Monique van Dijk, Frans P. M. Baar, Dick Tibboel, Birgit C. P. Koch, Teun van Gelder, Ron A. A. Mathot
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2018)
Article
Pharmacology & Pharmacy
Louise M. Andrews, Dennis A. Hesselink, Teun van Gelder, Birgit C. P. Koch, Elisabeth A. M. Cornelissen, Roger J. M. Bruggemann, Ron H. N. van Schaik, Saskia N. de Wildt, Karlien Cransberg, Brenda C. M. de Winter
CLINICAL PHARMACOKINETICS
(2018)