Article
Environmental Sciences
Michal Adamski, Eliza Zimolag, Ariel Kaminski, Justyna Drukala, Jan Bialczyk
Summary: Cyanotoxins produced by cyanobacteria pose significant threats to human keratinocytes, inhibiting proliferation and migration, and causing cytotoxic effects. Among them, CYN and its decomposition products exhibit strong inhibitory effects on keratinocytes, while ANTX-a shows lower toxicity, requiring higher concentrations and longer exposure. Further research on cyanotoxins should focus on their decomposition products and ecotoxicology in natural aquatic environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Ariel Kaminski, Christine Edwards, Ewelina Chrapusta-Srebrny, Linda A. Lawton
Summary: The study demonstrates that UV-A/TiO2 photocatalysis is effective in removing toxic substances such as ANTX-a and cyanobacterial cells, generating non-toxic decomposition products.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Chemistry, Analytical
Daniel G. Delafield, Gaoyuan Lu, Cameron J. Kaminsky, Lingjun Li
Summary: Mass spectrometry-based biomolecular analyses have become popular in research settings. Gas phase ion separation techniques provide a fourth dimension of analysis, enabling rapid characterization and high throughput profiling. This review highlights the latest developments and applications of high-end ion-mobility enabled instruments and their advantages for biomolecular research.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Ecology
Kristel F. Sanchez, Baili Zhong, Jorge A. Agudelo, Meghan A. Duffy
Summary: Transmission of parasites from one host to another is essential for their survival. For some aquatic parasites, transmission occurs through a free-living stage in the water, waiting for a new host. This study found that although common cyanobacterial toxins did not affect parasite infectivity and growth, the duration of time spent in the water impacted the parasite's ability to successfully infect new hosts.
FRESHWATER BIOLOGY
(2023)
Article
Environmental Sciences
Jutta Fastner, Jonna Teikari, Anja Hoffmann, Antje Kohler, Sebastian Hoppe, Elke Dittmann, Martin Welker
Summary: Fatal dog poisoning in Tegeler See, Berlin, Germany, caused by neurotoxic cyanobacteria associated with aquatic macrophytes has raised concerns about human exposure, especially for children, to cyanotoxins during recreational activities. A study conducted from 2017 to 2021 analyzed 398 macrophyte samples and 463 water samples from bathing sites in 19 Berlin lakes, detecting cyanotoxins in 66% of macrophyte samples and 50% of water samples. Anatoxins were the most frequently detected toxin group in macrophyte samples (58%), while cylindrospermopsins were most prevalent in water samples (41%). The study also identified the presence of anatoxin-producing cyanobacteria in the water moss Fontinalis antipyretica. Based on toxicological evaluations, a critical anatoxin content of 3 μg/g fresh weight in macrophyte samples is proposed.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemical Research Methods
Daniel G. Beach, Lydia Zamlynny, Melanie MacArthur, Christopher O. Miles
Summary: The study team developed an LC-HRMS/MS method for comprehensive detection of anatoxins (ATXs). The method was applied to samples of benthic cyanobacterial mats and laboratory cultures, leading to the discovery of several new ATXs. These new ATXs are mainly formed through nucleophilic addition reactions, similar to microcystins.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Karen E. Butler, Yoshihiko Takinami, Adam Rainczuk, Erin S. Baker, Blaine R. Roberts
Summary: Native mass spectrometry combined with IMS-MS technology can be used to study the tertiary and quaternary structure of proteins, validate other structural determination methods, and provide more comprehensive structural information.
FRONTIERS IN CHEMISTRY
(2021)
Article
Engineering, Environmental
Michal Saladyga, Malgorzata Kucala, Michal Adamski, Saravana Selvaraj, Ariel Kaminski
Summary: Phytoremediation is a useful method for removing cyanotoxins from water, but its efficacy is influenced by the composition of cyanobacterial mixtures. This study found that while some plants can effectively reduce intracellular toxins and cyanobacterial biomass, they can also cause an increase in toxin concentration in the medium.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
David S. Roberts, Morgan Mann, Jake A. Melby, Eli J. Larson, Yanlong Zhu, Allan R. Brasier, Song Jin, Ying Ge
Summary: Researchers have successfully elucidated the complete structure of newly discovered O-glycans on the S protein regional-binding domain (SR-BD) of the SARS-CoV-2 virus through a hybrid mass spectrometry approach. This method can provide insights into the interactions between the virus and host cells, laying a foundation for understanding the functional roles of O-glycans in SARS-CoV-2 and other glycoproteins.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Wojciech Fabianowski, Miroslaw Maziejuk, Monika Szyposzynska, Monika Wisnik-Sawka
Summary: The article presents a technique of differential ion mobility spectrometry (DMS) for the detection and identification of volatile organic compounds (VOCs) from various categories. It analyzes the possibility of mixture identification using only the DMS spectrometer and provides several examples. The article also discusses the analysis of different compounds, their mechanisms of fragmentation, influence on effective ion temperature, and high electric field intensity.
Review
Environmental Sciences
Simon Colas, Benjamin Marie, Emilie Lance, Catherine Quiblier, Helene Tricoire-Leignel, Cesar Mattei
Summary: ATX-a, a neurotoxic alkaloid produced by freshwater cyanobacteria, can cause neurologic symptoms in humans following food poisoning and death in wild and domestic animals. While acute effects and mode of action have been studied, little is known about its potential chronic toxicity.
ENVIRONMENTAL RESEARCH
(2021)
Article
Cell Biology
Malgorzata Kucala, Michal Saladyga, Ariel Kaminski
Summary: Lemna trisulca showed potential as a phytoremediation agent for reducing cyanotoxin concentrations in water through co-cultivation with different species of cyanobacteria, affecting their growth and development, and stabilizing water conductivity and pH values.
Article
Chemistry, Analytical
Sarah N. Sipe, James D. Sanders, Tobias Reinecke, Brian H. Clowers, Jennifer S. Brodbelt
Summary: New developments in analytical technologies and biophysical methods have advanced the characterization of increasingly complex biomolecular assemblies. Ion mobility methods, especially, provide a new dimension of structural information and protein analysis based on collision cross sections (CCSs). This article presents a modular drift tube design capable of separating native-like proteins with high resolution, and combines it with high-resolution Orbitrap MS for complex analysis. Additionally, the ability to rapidly determine accurate CCSs for proteins without the need for calibrants or correction factors is demonstrated.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Encarnacion Romera-Garcia, Rick Helmus, Ana Ballesteros-Gomez, Petra M. Visser
Summary: The increase in cyanobacterial blooms is linked to anthropogenic factors, causing concerns for drinking water companies and water managers due to the toxins produced. Simple, sensitive, and cost-effective analytical approaches are essential for risk assessment and large-scale studies. The development of a method for the multi-class determination of cyanotoxins in fresh water, which can separately report intra- and extracellular toxins, meets validation criteria and helps in risk management.
Article
Food Science & Technology
Andrew D. Turner, Florence R. I. Turner, Martha White, David Hartnell, Claire G. Crompton, Nicola Bates, Jan Egginton, Liz Branscombe, Adam M. Lewis, Benjamin H. Maskrey
Summary: This article reports a case study of a dog's death at a freshwater reservoir in SW England. Analysis revealed that anatoxins were responsible for the rapid death of the dog, with these neurotoxins affecting the nervous system and causing rapid death.
Article
Biochemical Research Methods
Asuncion Morales-Noe, Francesc A. Esteve-Turrillas, Sergio Armenta
Summary: Synthetic cannabinoids are a major group of new psychoactive substances with high potency effects, and their metabolism is usually entirely unknown. This study evaluated the metabolism of five third generation synthetic cannabinoids using zebrafish larvae as a model and proposed metabolic pathways for each compound. Zebrafish larvae, with genetic similarity to humans, are a useful tool for identifying the metabolic pathways of psychoactive substances.
DRUG TESTING AND ANALYSIS
(2022)
Article
Food Science & Technology
Antonio Abad-Fuentes, Consuelo Agullo, Daniel Lopez-Puertollano, Ismael Navarro-Fuertes, Antonio Abad-Somovilla, Josep Vicent Mercader
Summary: Appropriate hapten design and synthesis are critical for generating high-performance immunoreagents and developing sensitive and selective immunoanalytical methods. In this study, novel haptens of zearalenone were designed and evaluated for their structure-activity relationship. The results showed that modifying the hapten structure can affect antibody affinity and cross-reactivity, and changing the competing antigen structure can modify the immunoassay selectivity. These findings suggest that the immunochemical methods for zearalenone rapid analysis can still be improved in terms of sensitivity and selectivity.
Article
Chemistry, Analytical
Ramon E. Cevallos-Cedeno, Guillermo Quinones-Reyes, Consuelo Agullo, Antonio Abad-Somovilla, Antonio Abad-Fuentes, Josep Mercader
Summary: Sensitive and selective immunological assays for anatoxin-a have been developed using homemade monoclonal antibodies. The assays have low detection limits, good recovery rates, and are applicable in different formats. A visual detection method with a relatively low limit of detection has also been optimized. The accuracy of the method has been validated by comparison with high-performance liquid chromatography-tandem mass spectroscopy data.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Daniel Lopez-Puertollano, Consuelo Agullo, Josep V. Mercader, Antonio Abad-Somovilla, Antonio Abad-Fuentes
Summary: This study successfully developed high-affinity monoclonal antibodies against dicamba and used immunoassay technology to develop a method for the rapid determination of dicamba residues in environmental water samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Hadyn Duncan, Cristina Juan, Jordi Manes, Josep V. Mercader, Antonio Abad-Somovilla, Antonio Abad-Fuentes
Summary: A new method for the analysis of patulin in fruit products was developed in this study. The method is simple, sensitive, and does not require organic solvents. It has also been successfully applied under real conditions.
Article
Biochemical Research Methods
M. Munoz-Bartual, J. M. Herrero-Martinez, F. A. Esteve-Turrillas
Summary: A fast and simple method for synthesizing paper-immobilized molecularly imprinted polymers (MIPs) using a 405 nm laser pointer was proposed. The paper-MIP device allows the extraction and determination of methamphetamine in oral fluids and urine with high portability and minimal solvent consumption. The method provides a limit of detection of 14 μg L -1 and quantitative recoveries from 81 to 95%.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Evan Lesne, Miguel Munoz-Bartual, Francesc A. Esteve-Turrillas
Summary: A fast and simple procedure based on MEPS and LC-MS/MS was developed for quantifying 28 synthetic hallucinogens in oral fluids. The proposed method demonstrated high precision and quantitative recoveries, with limits of detection ranging from 0.09 to 1.22 µg/L. It is suitable for the determination of NBOMe derivatives and other synthetic hallucinogenic substances in oral fluid samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Correction
Food Science & Technology
Luis G. Addante-Moya, Antonio Abad-Somovilla, Antonio Abad-Fuentes, Consuelo Agullo, Josep V. Mercader
Article
Chemistry, Analytical
Simone Cavalera, Thea Serra, Antonio Abad-Fuentes, Josep V. Mercader, Antonio Abad-Somovilla, Fabio Di Nardo, Antonio D'Avolio, Amedeo De Nicolo, Valentina Testa, Matteo Chiarello, Claudio Baggiani, Laura Anfossi
Summary: Highly active antiretroviral therapy (HAART) can have toxic effects due to potent drugs like Tenofovir (TFV). Successful treatment relies on proper management of TFV, and therapeutic drug monitoring (TDM) of TFV concentrations in saliva can be a valuable tool for determining treatment compliance. We have developed a highly sensitive ELISA and LFIA for quantification and screening of TFV in saliva, respectively.
Article
Multidisciplinary Sciences
P. Garcia-Atienza, H. Martinez-Perez-Cejuela, E. F. Simo-Alfonso, J. M. Herrero-Martinez, S. Armenta
Summary: This study developed a liquid chromatography with fluorescence detection (LC-FD) method for analyzing 8 synthetic cannabinoids in oral fluids. The method was validated for linearity, precision, and extraction recoveries, with detection limits as low as 0.7 μg/L and quantification limits of 2.6 μg/L. Supel-Select HLB solid phase extraction cartridges provided quantitative recoveries for the extraction of synthetic cannabinoids in oral fluids.
Article
Food Science & Technology
Alejandro Garcia-Juan, Nuria Leon, Sergio Armenta, Olga Pardo
Summary: This study successfully developed and validated an analytical method for the simultaneous determination of ergot, tropane, and pyrrolizidine alkaloids in cereal-based food. The method showed high recoveries and precision, meeting the validation requirements. Analysis of reference materials further confirmed the reliability of the method. Analysis of actual samples revealed the presence of ergot alkaloids and pyrrolizidine alkaloids in some samples.
FOOD RESEARCH INTERNATIONAL
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
