期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1509, 期 -, 页码 69-82出版社
ELSEVIER
DOI: 10.1016/j.chroma.2017.06.017
关键词
Lipids; Lipidomics; Lipid fragmentation; Atmospheric pressure chemical ionization; Low resolution mass spectrometry; Human plasma
资金
- NSERC
A simple, fast, and versatile method, using an ultra-high performance liquid chromatography system coupled with a low resolution (single quadrupole) mass spectrometer was optimized to perform multiclass lipid profiling of human plasma. Particular attention was made to develop a method suitable for both electrospray ionization and atmospheric pressure chemical ionization interfaces (sequentially in positive- and negative-ion mode), without any modification of the chromatographic conditions (mobile phase, flow-rate, gradient, etc.). Emphasis was given to the extrapolation of the structural information based on the fragmentation pattern obtained using atmospheric pressure chemical ionization interface, under each different ionization condition, highlighting the complementary information obtained using the electrospray ionization interface, of support for related molecule ions identification. Furthermore, mass spectra of phosphatidylserine and phosphatidylinositol obtained using the atmospheric pressure chemical ionization interface are reported and discussed for the first time. (C) 2017 Elsevier B.V. All rights reserved.
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