Review
Immunology
Ayman F. Refaie, Batoul L. Elbassiouny, Malgorzata Kloc, Omaima M. Sabek, Sherry M. Khater, Amani M. Ismail, Rania H. Mohamed, Mohamed A. Ghoneim
Summary: MSC-based therapy for T1DM shows promise, but there are conflicting views on their immunological profile. Research focuses on understanding the mechanisms of their immunomodulatory functions and exploring ways to enhance their effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zahra Kargarpour, Jila Nasirzade, Layla Panahipour, Richard J. Miron, Reinhard Gruber
Summary: The study reveals that PRF lysates exhibit potent anti-inflammatory activity in murine mesenchymal cells, reducing cytokine-induced expression of inflammatory mediators and inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Zahra Kargarpour, Layla Panahipour, Michael Mildner, Richard J. Miron, Reinhard Gruber
Summary: Platelet-rich fibrin (PRF) has potent anti-inflammatory activity, however, the components responsible for this effect are unknown. In this study, we investigated whether the lipid fraction of PRF also exhibits anti-inflammatory properties. Our results show that lipids from PRF can significantly reduce the inflammatory response of ST2 bone marrow stromal cells and primary bone marrow macrophages. These findings suggest that the lipid fraction is at least partially responsible for the anti-inflammatory activity of PRF in vitro.
Article
Cell & Tissue Engineering
Mohamed A. Ghoneim, Mahmoud M. Gabr, Ayman F. Refaie, Sawsan M. El-Halawani, Mohga M. Al-issawi, Batoul L. Elbassiouny, Mai A. Abd El Kader, Amani M. Ismail, Mona F. Zidan, Mary S. Karras, Raghda W. Magar, Sherry M. Khater, Sylvia A. Ashamallah, Mahmoud M. Zakaria, Malgorzata Kloc
Summary: This study investigated allogenic immune responses following the transplantation of insulin-producing cells (IPCs) differentiated from human adipose tissue-derived stem cells (hAT-MSCs) into humanized mice. The results showed that the engrafted cells were not affected by allogenic immune response, suggesting potential for cell therapy for insulin-dependent diabetes without immunosuppression or encapsulation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Chemistry, Multidisciplinary
Byoungsu Ko, Jaekyung Kim, Younghwan Yang, Trevon Badloe, Jeonghoon Park, Joo Hwan Ko, Minsu Jeong, Hyunjung Kang, Chunghwan Jung, Young Min Song, Junsuk Rho
Summary: This study investigates humidity-responsive nano pixels with a 700 nm resolution, demonstrating full standard RGB gamut coverage and a millisecond-response time. The color pixels are designed as Fabry-Perot etalons and consist of an aluminum mirror substrate, humidity-responsive polyvinyl alcohol spacer, and a top layer of disordered silver nanoparticles. The experiment shows that the volume change of the polyvinyl alcohol reaches up to 62.5% when the relative humidity is manipulated from 20 to 90%. The disordered silver nanoparticle layer enhances the speed of absorption and swelling to the millisecond level.
Article
Cell & Tissue Engineering
O. Krasnova, A. Kovaleva, A. Saveleva, K. Kulakova, O. Bystrova, M. Martynova, A. Domnina, J. Sopova, I. Neganova
Summary: This study investigated the changes occurring within 3D-cultured MSCs and found that 3D-MSCs were able to lose replicative senescence markers, undergo changes in cytoskeleton structure and Golgi apparatus architecture, and exhibit nuclear localization of mTOR. The study also demonstrated that 3D cell culture promotes the phenotypic reversion of senescent cells rather than their removal from the population.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Polymer Science
Mario Alberto Perez-Diaz, Erick Jesus Martinez-Colin, Maykel Gonzalez-Torres, Carmina Ortega-Sanchez, Roberto Sanchez-Sanchez, Josselin Delgado-Meza, Fernando Machado-Bistrain, Valentin Martinez-Lopez, David Giraldo, Erik Agustin Marquez-Gutierrez, Jorge Armando Jimenez-Avalos, Zaira Yunuen Garcia-Carvajal, Yaaziel Melgarejo-Ramirez
Summary: This study aimed to investigate the biocompatibility and chondrogenic potential of a steam-sterilized Gel/CS/PVA hydrogel using differentiation of human adipose-derived mesenchymal stromal cells (AD-hMSC) and cartilage marker expression. The study found that AD-hMSC showed good biocompatibility on the hydrogel and was able to form chondrogenic nodules earlier compared to monolayer-differentiated cells. SEM analysis and expression of cartilage markers confirmed the improved potential of the hydrogel for chondrocyte differentiation.
Article
Cell & Tissue Engineering
Nesrine Ebrahim, Arigue A. Dessouky, Ola Mostafa, Amira Hassouna, Mohamed M. Yousef, Yasmin Seleem, Eman Abd El Aziz M. El Gebaly, Mona M. Allam, Ayman Samir Farid, Bayan A. Saffaf, Dina Sabry, Ahmed Nawar, Ahmed A. Shoulah, Ahmed H. Khalil, Sami F. Abdalla, Mohamed El-Sherbiny, Nehal M. Elsherbiny, Rabab F. Salim
Summary: The combined therapy of PRP and ADSCs significantly accelerated wound healing in experimentally induced diabetic rats by modulating the Notch signaling pathway, promoting angiogenesis, and enhancing EPSC proliferation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Niyaz Al-Sharabi, Reinhard Gruber, Mariano Sanz, Samih Mohamed-Ahmed, Einar K. Kristoffersen, Kamal Mustafa, Siddharth Shanbhag
Summary: The secretome of human bone marrow MSC (BMSC) was compared to that of leukocyte- and platelet-rich fibrin (L-PRF), in terms of wound healing and regeneration. The results showed that BMSC secretome had more differentially expressed proteins involved in wound healing, suggesting its potential for periodontal and bone regeneration. Further evaluation of the in vivo efficacy of this therapy is needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Chi B. Ahn, Ji-Hyun Lee, Joo H. Kim, Tae H. Kim, Hee-Sook Jun, Kuk H. Son, Jin W. Lee
Summary: This study developed a long-term, insulin-secreting, 3D-bioprinted construct to prevent islet loss in type 1 diabetes. The bioprinted construct showed improved insulin secretion and controlled glucose levels, resulting in higher survival rates compared to the non-implanted group.
BIO-DESIGN AND MANUFACTURING
(2022)
Review
Biochemistry & Molecular Biology
Ronit Vogt Sionov, Ronit Ahdut-HaCohen
Summary: Type 1 diabetes is a chronic autoimmune disease characterized by the destruction of insulin-producing beta-cells, leading to impaired glucose homeostasis. Mesenchymal stem cells (MSCs) have been found to support pancreatic islet function and protect islets through various mechanisms, including angiogenesis, immune response suppression, and secretion of growth factors.
Article
Biochemistry & Molecular Biology
Fabrizio Russo, Luca Ambrosio, Marianna Peroglio, Wei Guo, Sebastian Wangler, Jan Gewiess, Sibylle Grad, Mauro Alini, Rocco Papalia, Gianluca Vadala, Vincenzo Denaro
Summary: This pilot study evaluated the use of a hydrogel composed of HA and PRP as a carrier for hMSCs in intervertebral disc regeneration. The results showed that the hydrogel loaded with hMSCs maintained tissue structure, promoted cell activity, and enhanced NP marker expression. This study demonstrated the effectiveness of the HA/PRP hydrogel as a carrier for hMSCs in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biology
Seung Yeon Lee, Jin Woo Lee
Summary: This article reviews the recent advances in the application of 3D culture techniques for cartilage regeneration, focusing on the use of 3D spheroid culture system using stem cells. Exosomes, as intercellular communication systems, play a role in promoting chondrogenic differentiation and proliferation for cartilage repair. Further mechanistic studies are needed to enhance the therapeutic options for osteoarthritis using MSC-derived exosomes.
Article
Engineering, Biomedical
Aliaksandr Kraskouski, Kseniya Hileuskaya, Viktoryia Kulikouskaya, Volha Kabanava, Vladimir Agabekov, Sergei Pinchuk, Irina Vasilevich, Igor Volotovski, Tatyana Kuznetsova, Vasilina Lapitskaya
Summary: The study focuses on the design of novel wound dressings using mesenchymal stem cells (MSCs) immobilized on flexible polymer films for chronic wound treatment. Blended films based on polyvinyl alcohol (PVA) and pectin were prepared and evaluated for their physicochemical properties. Surface modification of PVA/pectin films increased their hydrophilicity and crosslinking with glutaraldehyde reinforced their structure, leading to effective adhesion of MSCs and potential application in wound dressings.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Cell Biology
Anne Wolff, Marcus Frank, Susanne Staehlke, Kirsten Peters
Summary: The cultivation conditions are crucial for the behavior of adMSC cells, as adipogenic differentiation only occurs under specific stimulation. Compared to 2D culture, the 3D culture environment has significant effects on adipogenic differentiation and adipokine release.
Article
Materials Science, Biomaterials
Hossein Mahboudi, Fatemeh Sadat Hosseini, Mousa Kehtari, Hadi Hassannia, Seyed Ehsan Enderami, Shahrzad Nojehdehi
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2020)
Article
Cell Biology
Mohammad Foad Abazari, Fatemeh Soleimanifar, Mojdeh Amini Faskhodi, Reyhaneh Nassiri Mansour, Javad Amini Mahabadi, Solmaz Sadeghi, Hadi Hassannia, Ehsan Saburi, Seyed Ehsan Enderami, Mohammad Mehdi Khani, Shohreh Zare Karizi
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Engineering, Biomedical
Aylin Tahmasebi, Seyed E. Enderami, Ehsan Saburi, Maryam Islami, Somayeh Yaslianifard, Javad A. Mahabadi, Abdolreza Ardeshirylajimi, Fatemeh Soleimanifar, Abbas S. Moghadam
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2020)
Article
Biochemistry & Molecular Biology
Javad Amini Mahabadi, Abolfazl Aazami Tameh, Sayyed Alireza Talaei, Mohammad Karimian, Tahereh Rahiminia, Seyed Ehsan Enderami, Seyed Mohammad Gheibi Hayat, Hossein Nikzad
JOURNAL OF CELLULAR BIOCHEMISTRY
(2020)
Article
Cell Biology
Mohammad Foad Abazari, Navid Nasiri, Fatemeh Nejati, Shohreh Zare Karizi, Mojdeh Amini Faskhodi, Ehsan Saburi, Nasrin Aghapur, Mohammad Reza Mahdavi, Abdolreza Ardeshirylajimi, Seyed Ehsan Enderami, Fatemeh Soleimanifar
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Engineering, Biomedical
Aylin Tahmasebi, Abbas Shapouri Moghadam, Seyed Ehsan Enderami, Maryam Islami, Mohamad Kaabi, Ehsan Saburi, Amir Daei Farshchi, Fatemeh Soleimanifar, Vahid Mansouri
Article
Genetics & Heredity
Mohammad Foad Abazari, Zahrasadat Hosseini, Shohreh Zare Karizi, Sara Norouzi, Mojdeh Amini Faskhoudi, Ehsan Saburi, Seyed Ehsan Enderami, Abdolreza Ardeshirylajimi, Hassan Mohajerani
Article
Materials Science, Biomaterials
Seyed Ehsan Enderami, Seyedeh Fatemeh Ahmadi, Reyhaneh Nassiri Mansour, Saeid Abediankenari, Hossein Ranjbaran, Majid Mossahebi-Mohammadi, Reza Salarinia, Hossein Mahboudi
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Review
Cell & Tissue Engineering
Elham Sabouri, Alireza Rajabzadeh, Seyedeh Elnaz Enderami, Ehsan Saburi, Fatemeh Soleimanifar, Ghasem Barati, Mohamad Rahmati, Gholamreza Khamisipour, Seyed Ehsan Enderami
Summary: Stem cell-based therapy shows promising results in treating diabetes by inducing insulin-producing cells from various sources, which can serve as an alternative to islets. The use of soluble growth factors, small molecules, gene-encoding transcription factors, and microRNAs for stem cell differentiation has been explored, with a focus on miRNAs and their roles in pancreatic development and function.
CURRENT STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Omid Baradarian Ghanbari, Masoud Soleimani, Minoo Shahidi, Mohammad Adel Ghiass, Seyed Ehsan Enderami, Akbar Dorgalaleh
Summary: In this study, a natural acellular scaffold derived from rat lung and a 3D bioreactor were used to differentiate hiPSCs into megakaryocytes. The results demonstrated the successful differentiation process and indicated that the approach provided a suitable microenvironment for cell differentiation.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Polymer Science
Elham Sabouri, Zahra Rezaie, Seyed Ehsan Enderami, Mahdi Mirahmadi, Mohammad Askari
Summary: Electrospinning technique was used to fabricate PLLA/PHB nanofibrous scaffolds, with two-nozzle approach showing improved biocompatibility and osteoconductive capacity compared to single-nozzle method. The study suggests that two-nozzle electrospinning could enhance the potential of these scaffolds for bone tissue engineering applications.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2021)
Article
Genetics & Heredity
Mohammad Foad Abazari, Shohreh Zare Karizi, Nazanin Hajati-Birgani, Sara Norouzi, Zahraalsadat Khazeni, Javad Hashemi, Lida Shafaghi, Fatemeh Soleimanifar, Reyhaneh Nassiri Mansour, Seyed Ehsan Enderami
Summary: The use of PHBV nanofibers as scaffolds promoted the differentiation of induced pluripotent stem cells (iPSCs) into insulin-producing cells (IPC) for diabetic treatment. iPSCs cultured on PHBV nanofibers showed significantly higher survival rates, increased insulin and C-peptide secretion levels, and upregulation of genes and insulin protein expression compared to cells cultured on traditional tissue culture polystyrene (TCPS).
Article
Engineering, Biomedical
Reyhaneh Nassiri Mansour, Ayoob Karimizade, Seyed Ehsan Enderami, Mozhgan Abasi, Fereshteh Talebpour Amiri, Abdolreza Jafarirad, Amir Mellati
Summary: This study investigated the effects of animal age, decellularization protocols, and sterilization methods on xenogeneic acellular dermal matrices (ADMs) for skin regeneration. The results show that fetal source and EO-sterilized samples performed better in terms of morphology, mechanical properties, and wound treatment. There was no added value in using enzymatic treatment during the decellularization process. The findings suggest that the selection of animal age, decellularization, and sterilization methods should be based on clinical requirements.
Article
Engineering, Biomedical
Seyedeh Fatemeh Ahmadi, Reyhaneh Nassiri Mansour, Hadi Hassannia, Seyed Ehsan Enderami, Saeid Abediankenari, Zahra Hosseini-khah
Summary: This study evaluated the differentiation of iPSCs into IPCs on a 3D scaffold and compared it with a 2D cultured group. The results showed that the differentiated cells on the 3D scaffold had higher expression of pancreatic specific-markers and released more insulin and C-peptide compared to the 2D group.
Article
Genetics & Heredity
Reyhaneh Nassiri Mansour, Elham Hasanzadeh, Mozhgan Abasi, Mazaher Gholipourmalekabadi, Amir Mellati, Seyed Ehsan Enderami
Summary: Combining fetal bovine acellular dermal matrix (FADM) with human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) can promote the healing of full-thickness skin wounds. The FADM-WJMSC group showed a higher wound closure rate and fewer inflammatory cells compared to the control and FADM groups. These findings suggest the potential application value of combining FADM with hWJ-MSCs in the treatment of skin wounds.