Article
Biochemistry & Molecular Biology
Eunjin Cho, Seongmin Cheon, Mina Ding, Kayeong Lim, Sang-Wook Park, Chungoo Park, Tae-Hoon Lee
Summary: In this study, the fusion-specific genes involved in osteoclastogenesis were identified using RNA sequencing and confirmed their importance through gene editing. These findings provide potential targets for osteoporosis therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jun Sugimoto, Sehee Choi, Megan A. Sheridan, Iemasa Koh, Yoshiki Kudo, Danny J. Schust
Summary: Proper placental development relies on tightly regulated trophoblast differentiation and interaction with maternal cells. Human endogenous retroviruses play an integral role in modulating cell fusion events in the trophoblast cells of the developing placenta. Variations in HERV expression levels may contribute to adverse pregnancy outcomes by affecting fusion and anti-fusogenic events in the placenta.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Maribel Lara-Chica, Alejandro Correa-Saez, Rafael Jimenez-Izquierdo, Martin Garrido-Rodriguez, Francisco J. Ponce, Rita Moreno, Kimberley Morrison, Chiara Di Vona, Krisztina Arato, Carla Jimenez-Jimenez, Rosario Morrugares, M. Lienhard Schmitz, Susana de la Luna, Laureano de la Vega, Marco A. Calzado
Summary: DYRK2 is a serine/threonine kinase that phosphorylates CDC25A to control its degradation, while CDC25A can influence the phosphorylation status of DYRK2, forming a feedback regulatory loop that plays a key role in cell cycle regulation and genomic integrity.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Fisheries
Shanshan Zeng, Meifeng Li, Xining Cheng, Shina Lu, Zhiqing Feng, Zeyin Jiang, Zhichao Sun, Xiaowen Xu, Huiling Mao, Chengyu Hu
Summary: In this study, it was found that grass carp DYRK2 induces cell apoptosis by increasing the phosphorylation level of p53.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Malgorzata Kloc, Arijita Subuddhi, Ahmed Uosef, Jacek Z. Kubiak, Rafik M. Ghobrial
Summary: Cell fusion occurs in various biological situations, especially in cells of the monocyte-macrophage lineage, which are highly fusogenic and can form multinucleated giant cells. Fusion of monocytes/macrophages with tumor cells may also promote cancer metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Zhuhao Wu, Bin Chen, Yue Wu, Yu Xia, Hui Chen, Zhiyi Gong, Hang Hu, Zhao Ding, Shishang Guo
Summary: A scaffold-free acoustofluidic device was developed to fabricate heterotypic cell spheroids with complexity over cell architectures and components, demonstrating potential in various areas such as tumor research, tissue engineering, developmental biology, and drug discovery.
Review
Biochemistry & Molecular Biology
Vasudha Tandon, Laureano de la Vega, Sourav Banerjee
Summary: DYRK2 has been reported as a tumor suppressor in various cancers, but inhibitors of DYRK2 have shown robust antiproliferative properties. DYRK2 regulates proteostasis in cancer and promotes survival in specific cancers.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biophysics
Bipul R. Acharya
Summary: Interactions between cells exchanging biomechanical signals are crucial for organ physiology and pathogenesis, with each cell lineage responding differently to specific signals. The form and magnitude of mechanical forces impact biochemical signaling in a cell-specific manner.
JOURNAL OF BIOMECHANICS
(2021)
Article
Oncology
Dylan L. Schaff, Shambhavi Singh, Kee-Beom Kim, Matthew D. Sutcliffe, Kwon-Sik Park, Kevin A. Janes
Summary: This study reveals that the single-cell regulatory heterogeneity of small-cell lung cancer becomes increasingly complex in the liver, a common metastatic site. Heterotypic interactions in the liver and lung may accelerate intratumor heterogeneity in small-cell lung cancer. These findings provide insights into the progression and complexity of small-cell lung cancer metastasis.
Article
Cell Biology
Nathaniel R. Campbell, Anjali Rao, Miranda Hunter, Magdalena K. Sznurkowska, Luzia Briker, Maomao Zhang, Maayan Baron, Silja Heilmann, Maxime Deforet, Colin Kenny, Lorenza P. Ferretti, Ting-Hsiang Huang, Sarah Perlee, Manik Garg, Jeremie Nsengimana, Massimo Saini, Emily Montal, Mohita Tagore, Julia Newton-Bishop, Mark R. Middleton, Pippa Corrie, David J. Adams, Roy Rabbie, Nicola Aceto, Mitchell P. Levesque, Robert A. Cornell, Itai Yanai, Joao B. Xavier, Richard M. White
Summary: Melanomas can have multiple coexisting cell states, including proliferative (PRO) versus invasive (INV) subpopulations that cooperate in the seeding of metastasis. INV cells tightly adhere to each other and form clusters with a rim of PRO cells, facilitating dissemination of less metastatic PRO cells. TFAP2 neural crest transcription factor is identified as a master regulator of clustering and PRO/INV states.
DEVELOPMENTAL CELL
(2021)
Editorial Material
Cell Biology
William T. Frantz, Craig J. Ceol
Summary: This study demonstrates the critical role of interactions between heterogeneous melanoma cells in promoting collective extravasation and seeding the growth of metastatic lesions. Such findings emphasize the importance of heterotypic clusters in the metastasis process.
DEVELOPMENTAL CELL
(2021)
Article
Oncology
Magdalena Rausch, Lea Blanc, Olga De Souza Silva, Olivier Dormond, Arjan W. Griffioen, Patrycja Nowak-Sliwinska
Summary: The study showed that heterotypic short-term 3D spheroids combined with immune cell subsets provide a valuable tool for simulating tumor-immune cell interactions and drug screening. Compared to 2D cell culture platforms, this model better reflects the complex tumor microenvironment, aiding in evaluating treatment response and cancer cell fate.
Article
Medicine, Research & Experimental
Ranli Gu, Hao Liu, Menglong Hu, Yuan Zhu, Xuenan Liu, Feilong Wang, Likun Wu, Danyang Song, Yunsong Liu
Summary: This study found that D-mannose can protect rats from bone loss and urinary tract infections under weightlessness. The bone protective effects of D-mannose are mediated by inhibiting osteoclast cell fusion.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Stephani Edwina Lucia, Hyuntae Jeong, Jennifer H. Shin
Summary: Establishing clear boundaries between different types of cells is crucial for tissue development and regeneration. Recent studies have found that cortical actomyosin structures play a key role in maintaining these boundaries. By examining the physical properties of cells, researchers discovered that collisions and repulsions between different cell types can accumulate stress and establish stable boundaries.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Multidisciplinary Sciences
Yun-Jeong Choe, Jin Young Min, Hyunseong Lee, Sang-Yeop Lee, Joseph Kwon, Hye-Jin Kim, Jangho Lee, Hyun Min Kim, Hye Sun Park, Mi Young Cho, Ju-Yong Hyun, Hye Min Kim, Yong-Ho Chung, Sang Keun Ha, Hye Gwang Jeong, Inpyo Choi, Tae-Don Kim, Kwan Soo Hong, Eun Hee Han
Summary: This study observed heterotypic cell-cell interactions in tumor tissues, showing their association with immune evasion and drug resistance in cancer cells.
Article
Engineering, Biomedical
Shani Felder, Hila Masasa, Ayelet Orenbuch, Noam Levaot, Michal Shachar Goldenberg, Smadar Cohen
Article
Dentistry, Oral Surgery & Medicine
Eitan Bar Droma, Guy Beck-Rosen, Anatoly Ilgiyaev, Yariv Fruchtman, Chen Abramovitch-Dahan, Noam Levaot, Navot Givol
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
(2020)
Review
Endocrinology & Metabolism
Milena Jovanovic, Gali Guterman-Ram, Joan C. Marini
Summary: Osteogenesis imperfecta (OI) is a diverse genetic and phenotypic skeletal disorder characterized by bone fragility, growth deficiency, and skeletal deformity. Research focuses on defects in collagen, abnormal bone mineralization, different inheritance patterns, and various genetic mutations related to OI.
Article
Biochemistry & Molecular Biology
Svetlana Katchkovsky, Biplab Chatterjee, Chen-Viki Abramovitch-Dahan, Niv Papo, Noam Levaot
Summary: The binding of sclerostin (Scl) to its receptors, low-density lipoprotein receptor-related proteins 5 or 6 (LRP5/6), inhibits the induction of bone formation by Wnt ligands. It has been recently discovered that Scl binding to the co-receptor LRP4 enhances this inhibition. Using the yeast display system, this study demonstrates that Scl has a stronger binding affinity for LRP4 than for LRP6, and the presence of LRP4 enhances Scl binding to LRP6. Furthermore, the study shows that the binding of Scl to LRP4 is crucial for its inhibition of the Wnt pathway. The findings suggest that compounds targeting Scl-LRP4 interactions could be potential strategies for promoting anabolic bone functions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Editorial Material
Cell Biology
Yankel Gabet, Drorit Neumann, Noam Levaot, Ari Elson, Natalie A. Sims
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Ghazal Hedjazi, Gali Guterman-Ram, Stephane Blouin, Victoria Schemenz, Wolfgang Wagermaier, Peter Fratzl, Markus A. Hartmann, Jochen Zwerina, Nadja Fratzl-Zelman, Joan C. Marini
Summary: This study characterized bone tissue in a novel mouse model of atypical type VI osteogenesis imperfecta (OI) and found that the model exhibited hypermineralization of the matrix, increased osteocyte porosity, disrupted lacuno-canalicular network, and disordered collagen fibril orientation.
Article
Endocrinology & Metabolism
Ahmed El-Gazzar, Heeseog Kang, Nadja Fratzl-Zelman, Emma Webb, Aileen M. Barnes, Milena Jovanovic, Sarju G. Mehta, Vipan Datta, Vrinda Saraff, Ryan K. Dale, Frank Rauch, Joan C. Marini, Wolfgang Hoegler
Summary: Loss-of-function mutations in SMAD3 cause Loeys-Dietz syndrome type 3 (LDS3), a rare connective tissue disorder characterized by vascular pathology and skeletal abnormalities. This study investigated bone histomorphometry and matrix mineralization in human bone with a SMAD3 mutation, and explored the cellular defect associated with the TGF-beta/SMAD pathway.
Article
Genetics & Heredity
Max Drabkin, Matan M. Jean, Yael Noy, Daniel Halperin, Yuval Yogev, Ohad Wormser, Regina Proskorovski-Ohayon, Vadim Dolgin, Noam Levaot, Vlad Brumfeld, Shira Ovadia, Mor Kishner, Udi Kazenell, Karen B. Avraham, Ilan Shelef, Ohad S. Birk
Summary: This study identifies a variant in the SMARCA4 gene as a possible cause of otosclerosis, leading to hearing impairment and abnormal bone formation in the auditory bullae. Through genetic studies and CRISPR mutant mouse models, researchers confirmed the association between this variant and otosclerosis. This finding is significant for understanding the molecular genetic pathophysiology of otosclerosis and developing treatment strategies.
JOURNAL OF MEDICAL GENETICS
(2023)
Meeting Abstract
Endocrinology & Metabolism
Sveta Katchkovsky, Noam Levaot, Niv Papo
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Yaron Meirow, Milena Jovanovic, Yuval Zur, Juliana Habib, Daniele Filippo Colombo, Nira Twaik, Hadas Ashkenazi-Preiser, Kerem Ben-Meir, Ivan Mikula, Or Reuven, Guy Kariv, Leonor Daniel, Saja Baraghithy, Yehuda Klein, Jeroen Krijgsveld, Noam Levaot, Michal Baniyash
Summary: This study identified two distinct subsets of osteoclast precursors (OCPs) and revealed their different response to inflammatory cues and their role in inflammatory bone loss (IBL). The findings indicate that the Ly6C(hi)CD11b(hi) inflammatory OCPs (iOCPs) play a central role in IBL induction while the Ly6C(hi)CD11b(lo) homeostatic OCPs (hOCPs) remain stable and less affected by inflammation. This study provides insights into the understanding of IBL and suggests iOCPs as potential biomarkers and therapeutic targets for IBL.
Meeting Abstract
Endocrinology & Metabolism
Svetlana Katchkovsky, Niv Papo, Noam Levaot
JOURNAL OF BONE AND MINERAL RESEARCH
(2020)
Meeting Abstract
Endocrinology & Metabolism
Yuval Zur, Lior Rosenfeld, Gali Guterman-Ram, Niv Papo, Noam Levaot
JOURNAL OF BONE AND MINERAL RESEARCH
(2018)