Article
Biochemistry & Molecular Biology
Di Sun, Jiaqi An, Zixu Cui, Jiao Li, Ziwei You, Chang Lu, Yang Yang, Pengfei Gao, Xiaohong Guo, Bugao Li, Chunbo Cai, Guoqing Cao
Summary: This study identifies a novel circRNA, circCSDE1, that is highly expressed in skeletal muscle. Functional studies show that circCSDE1 promotes proliferation and inhibits myoblast differentiation, while miR-21-3p has opposite effects. Dual-luciferase reporter assay confirms that circCSDE1 targets miR-21-3p directly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xianxian Zhang, Shihui Huang, Xi Niu, Sheng Li, Jiafu Wang, Xueqin Ran
Summary: In this study, it was discovered that miR-103-3p is highly expressed in skeletal muscle and has inhibitory effects on myotube formation and differentiation of C2C12 cells. Furthermore, miR-103-3p was found to directly target the MAP4 gene and regulate autophagy in myoblasts. These findings contribute to a better understanding of the regulatory role of miRNAs in skeletal muscle myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Meng Li, Na Zhang, Jiao Li, Mengting Ji, Tianzhi Zhao, Jiaqi An, Chunbo Cai, Yang Yang, Pengfei Gao, Guoqing Cao, Xiaohong Guo, Bugao Li
Summary: Muscle development is influenced by circRNAs, which play a key role in regulating myogenesis. The study investigated circRNA profiles in skeletal muscle of Mashen and Large White pigs, and identified 362 differentially expressed circRNAs, including circIGF1R. Functional experiments revealed that circIGF1R promoted myoblast differentiation, but had no effect on cell proliferation. It was found that circIGF1R acted as a sponge for miR-16, and counteracted its inhibitory effect on myoblast differentiation. This study provides insights into the mechanism of circRNAs in regulating porcine myoblast differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
ZhongLin Tang, YiLong Yao, YongSheng Zhang, ZiShuai Wang, Dan Lu, YuanYuan Zhang, Adeyinka Abiola Adetula, SiYuan Liu, Min Zhu, YaLan Yang, XinHao Fan, MuYa Chen, YiJie Tang, Yun Chen, YuWen Liu, GuoQiang Yi
Summary: This study revealed the role of miR-743a-5p in regulating myoblast differentiation through targeting Mob1b and explored the mechanisms of skeletal muscle development and regeneration.
Article
Biochemistry & Molecular Biology
Jen-Hao Yang, Ming-Wen Chang, Dimitrios Tsitsipatis, Xiaoling Yang, Jennifer L. Martindale, Rachel Munk, Aiwu Cheng, Elizabeth Izydore, Poonam R. Pandey, Yulan Piao, Krystyna Mazan-Mamczarz, Supriyo De, Kotb Abdelmohsen, Myriam Gorospe
Summary: In this study, the researchers discovered a noncoding RNA regulatory network that promotes myoblast fusion into multinucleated myotubes by modulating the degradation of miR-7.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Physiology
Chrystalla Mytidou, Andrie Koutsoulidou, Margarita Zachariou, Marianna Prokopi, Konstantinos Kapnisis, George M. Spyrou, Andreas Anayiotos, Leonidas A. Phylactou
Summary: The study revealed that the expression of the four myomiRs in skeletal muscle tissues of mice changed with age, with miR-1, miR-133a, and miR-133b increasing rapidly before adolescence, while miR-206 decreased in all muscles. Furthermore, levels of miR-1, miR-133a, and miR-133b in muscle-derived exosomes peaked before early adolescence.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Mengjin Gao, Xue Li, Zuojun Yang, Shuo Zhao, Xingxing Ling, Jingjing Li, Kai Xing, Xiaolong Qi, Xiangguo Wang, Longfei Xiao, Hemin Ni, Yong Guo, Xihui Sheng
Summary: This study reveals that circHIPK3 regulates skeletal muscle development through the miR-7/TCF12 pathway, providing important insights into the biological mechanisms involved.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Paula Gutierrez-Perez, Emilio M. Santillan, Thomas Lendl, Jingkui Wang, Anna Schrempf, Thomas L. Steinacker, Mila Asparuhova, Marlene Brandstetter, David Haselbach, Luisa Cochella
Summary: The study reveals the vulnerability of muscle cells by miR-1, which represses multiple subunits of the V-ATPase complex, impacting muscle function. This sheds light on a previously unknown role for microRNAs in regulating protein complexes and V-ATPase assembly as a conserved muscle cell vulnerability.
Article
Biochemistry & Molecular Biology
Ailma O. da Paixao, Anaysa Paola Bolin, Joao G. Silvestre, Alice Cristina Rodrigues
Summary: The saturated fatty acid palmitic acid negatively affects muscle tube diameter, fusion, and metabolism, which may be related to miR-206.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Shan Wang, Xiukai Cao, Ling Ge, Yifei Gu, Xiaoyang Lv, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun
Summary: This study conducted functional verification of miRNA in Hu sheep skeletal muscle cells and found that miR-22-3p plays a role in the proliferation and differentiation of Hu sheep skeletal muscle cells by targeting IGFBP3.
Article
Biology
Shurong Liu, Shujuan Xie, Huafeng Chen, Bin Li, Zhirong Chen, Yeya Tan, Jianhua Yang, Lingling Zheng, Zhendong Xiao, Qi Zhang, Lianghu Qu
Summary: The study identified miR-101a/b as a pair of non-muscle-specific miRNAs transiently upregulated during myogenesis, which inhibit myoblast differentiation by suppressing p38/MAPK, Interferon Gamma, and Wnt pathways while enhancing the C/EBP pathway. The interaction between miR-101a/b and the long non-coding RNA Malat1 competes with Mef2a mRNA to relieve inhibition of the p38/MAPK pathway, revealing a new regulatory mechanism in myoblast differentiation.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yongqi Yue, Xinxin Feng, Yige Jia, Shujie Luo, Menglei Jiang, Jiandan Luo, Yonglin Hua, Jiyue Zhang, Yaqiu Lin, Jian Li, Yan Xiong
Summary: MiR-424(322)-5p is highly expressed in mouse skeletal muscle and has been found to inhibit the proliferation and differentiation of myoblasts by targeting Ezh1. This study provides new insights into the role of miRNAs in muscle development and related diseases.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Article
Genetics & Heredity
Natalia Leciejewska, Ewa Pruszynska-Oszmalek, Karolina Mielnik, Maciej Glowacki, Tomasz P. Lehmann, Maciej Sassek, Bartosz Gaweda, Dawid Szczepankiewicz, Krzysztof W. Nowak, Pawel A. Kolodziejski
Summary: SPX and its receptors, GalR2 and GalR3, have significant effects on the metabolism, proliferation, and differentiation of skeletal muscle cells. Through modulation of protein expression and cell signaling pathways, SPX promotes the proliferation and differentiation of C2C12 cells, as well as myoblast fusion into myotubules. Additionally, exercise stimulates the expression of SPX and GalR2, leading to an increase in SPX concentration in the blood serum.
Article
Biochemistry & Molecular Biology
Duk-Hwa Kwon, Joo-Young Kang, Hosouk Joung, Ji-Young Kim, Anna Jeong, Hyun-Ki Min, Sera Shin, Yun-Gyeong Lee, Young-Kook Kim, Sang-Beom Seo, Hyun Kook
Summary: The balance between KDM2B and SET7, two competing enzymes that respectively inhibit and activate the same gene-regulating protein SRF, plays a critical role in skeletal muscle cell differentiation. KDM2B inhibits cell differentiation by demethylating SRF, while SET7 activates differentiation by methylating SRF. This study reveals SRF as a novel target of KDM2B and provides insights into skeletal muscle development in health and disease.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
Chengchuang Song, Xue Fang, Zhaoxin Yang, Qi Wang, Fantong Meng, Yaqi Chen, Junhao Chen, Bei Zhao, Yanhong Wang, Xingtang Fang, Lihong Gu, Chunlei Zhang
Summary: Our study shows that miR-152 inhibits proliferation of bovine myoblasts by targeting KLF6, which has certain effects on the development of bovine skeletal muscles.