期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 119, 期 2, 页码 2200-2211出版社
WILEY
DOI: 10.1002/jcb.26382
关键词
hydronephrosis; renal interstitial fibrosis; renal tubular damage; silencing; suppressor of cytokine signaling-1; 3
资金
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- 333 Project Award of Jiangsu Province
- Qinglan Project of the Young and Middle-aged Academic Leader of Jiangsu College and University
- National Natural Science Foundation of China [81571055, 81400902, 81271225, 31201039, 81171012]
- Major Fundamental Research Program of the Natural Science Foundation of the Jiangsu Higher Education Institutions of China [13KJA180001]
- Cultivate National Science Fund for Distinguished Young Scholars of Jiangsu Normal University
- Graduate Student Innovation Program of Jiangsu Province [KYZZ16_0467]
Our study was performed to elucidate how SOCS-1/3 silencing suppresses renal interstitial fibrosis (RIF) by alleviating renal tubular damage in rat models affected by hydronephrosis. Male Wistar rats were randomly selected to establish hydronephrosis rat model, after which all rats were classified into normal, model, negative control (NC), siRNA-SOCS-1, siRNA-SOCS-3, and siRNA-SOCS-1+siRNA-SOCS-3 groups. The levels of urine protein, serum creatinine (Scr), and blood urea nitrogen (BUN) were detected. ELISA was performed to determine levels of cystatin (CysC), 2-microglobulin (2-MG), interleukin (IL)-6, and tumor necrosis factor (TNF)-. RT-qPCR and Western blotting were used for mRNA and protein expressions of SOCS-1, SOCS-3, -smooth muscle actin (-SMA), and transforming Growth Factor (TGF)-1. Compared with the normal group, the levels of Scr, BUN, urine protein, NAG, CysC, 2-MG, IL-6, and TNF- were increased in other groups, as well as elevated mRNA and protein expressions of SOCS-1, SOCS-3, -SMA, and TGF-1. The siRNA-SOCS-1, siRNA-SOCS-3, and siRNA-SOCS-1+siRNA-SOCS-3 groups were found with decreased levels of Scr, BUN, urine protein, NAG, CysC, 2-MG, IL-6, and TNF-, as well as mRNA and protein expressions of SOCS-1, SOCS-3, -SMA, and TGF-1, including positive rates of SOCS-1 and SOCS-3 proteins in comparison with the model and NC groups. In comparison with the siRNA-SOCS-1 and siRNA-SOCS-3 groups, the siRNA-SOCS-1+siRNA-SOCS-3 group exhibited decreased levels of Scr, BUN, urine protein, NAG, CysC, 2-MG, IL-6, and TNF-. Our study demonstrated that silencing of SOCS-1/3 may suppress RIF by alleviating the renal tubular damage in rat models affected by hydronephrosis.
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