期刊
JOURNAL OF CELL SCIENCE
卷 130, 期 24, 页码 4108-4119出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.207134
关键词
Cytokinesis; CIF1; CIF2; Zinc finger; EF hand; Trypanosoma brucei
类别
资金
- National Institutes of Health [AI101437]
- Austrian Science Fund (FWF) [P28231-B28]
- Austrian Science Fund (FWF) [P28231] Funding Source: Austrian Science Fund (FWF)
Cytokinesis in trypanosomes occurs uni-directionally along the longitudinal axis from the cell anterior towards the cell posterior and requires a trypanosome-specific CIF1-CIF2 protein complex. However, little is known about the contribution of the structural motifs in CIF1 and CIF2 to complex assembly and cytokinesis. Here, we demonstrate that the two zinc-finger motifs but not the coiled-coil motif in CIF1 are required for interaction with the EF-handmotifs in CIF2. We further show that localization of CIF1 depends on the coiled-coil motif and the first zinc-finger motif and that localization of CIF2 depends on the EF-hand motifs. Deletion of the coiled-coil motif and mutation of either zinc-finger motif in CIF1 disrupts cytokinesis. Furthermore, mutation of either zinc-finger motif in CIF1 mislocalizes CIF2 to the cytosol and destabilizes CIF2, whereas deletion of the coiled-coil motif in CIF1 spreads CIF2 over to the new flagellum attachment zone and stabilizes CIF2. Together, these results uncover the requirement of the coiled-coil and zinc-finger motifs for CIF1 function in cytokinesis and for CIF2 localization and stability, providing structural insights into the functional interplay between the two cytokinesis regulators.
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