Review
Biochemistry & Molecular Biology
Moaz Ahmad, Sasirekha Narayanasamy, Hwei Ling Ong, Indu Ambudkar
Summary: ER-PM junctions, formed by the endoplasmic reticulum and plasma membrane, play a crucial role in assembling calcium signaling complexes. The formation and stability of these junctions rely on the enrichment of specific proteins, which contribute to junctional stability and regulate the lipid levels of ER and plasma membranes.
Article
Biochemistry & Molecular Biology
Woori Ko, Byung-Chang Suh
Summary: The membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) selectively modulates the gating of the TMEM16A(ac) channel in an agonistic manner, leading to the upregulation of TMEM16A(ac) functions in physiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Maximilian M. Gass, Sarah Borkowsky, Marie-Luise Lotz, Rebecca Siwek, Rita Schroeter, Pavel Nedvetsky, Stefan Luschnig, Astrid Rohlmann, Markus Missler, Michael P. Krahn
Summary: Drosophila nephrocytes, similar to mammalian podocytes, exhibit characteristics of epithelial cells. Research has shown that phospholipid PI(4,5)P2 plays a crucial role in slit diaphragm formation, while PI(3,4,5)P3 has minimal impact on nephrocyte function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Alex G. Batrouni, Nirmalya Bag, Henry T. Phan, Barbara A. Baird, Jeremy M. Baskin
Summary: Phosphatidylinositol 4-kinase III alpha (PI4KIII alpha) is responsible for generating phosphatidylinositol (4)-phosphate at the plasma membrane, and the differential palmitoylation patterns of its anchor protein EFR3B control its distribution and phosphoinositide homeostasis.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biology
Stefano Perni, Kurt Beam
Summary: Neuronal junctophilins JPH3 and JPH4 play important roles in the junctions between the endoplasmic reticulum and plasma membrane, interacting differently with various calcium channels and modifying the inactivation rate of Ca(V)2.1 and Ca(V)2.2.
Article
Biochemistry & Molecular Biology
Luis Borges-Araujo, Marina E. Monteiro, Dalila Mil-Homens, Nuno Bernardes, Maria J. Sarmento, Ana Coutinho, Manuel Prieto, Fabio Fernandes
Summary: Despite its low abundance, PI(4,5)P-2 plays a key role in membrane-associated signaling events in eukaryotic cells. The presence of physiological divalent cations can affect the clustering of PI(4,5)P-2 and its interactions with proteins, which can have a significant impact on the organization and downstream interactions of PI(4,5)P-2-binding proteins in the plasma membrane. This study characterizes the effects of Ca2+ on the organization and protein-protein interactions of PI(4,5)P-2-binding proteins, providing insights into the complex regulation of PI(4,5)P-2 in cell signaling.
Article
Multidisciplinary Sciences
Woo Young Chung, Malini Ahuja, Beth A. McNally, Spencer R. Leibow, Henry K. E. Ohman, Ava Movahed Abtahi, Shmuel Muallem
Summary: Membrane contact site ER/PM junctions play a crucial role in cellular signaling, including Ca2+ signaling. Phosphatidylserine (PtdSer) regulates the composition of these junctions and is involved in Ca2+ signaling, Ca2+-dependent gene regulation, and cellular functions. ORP5 and ORP8 mediate the exchange of PtdSer and PI(4)P at the ER/PM junctions, and their differential lipid exchange modes determine the PtdSer/PI(4)P ratio, which controls the interaction between STIM1-STIM1 and STIM1-Orai1, the activity of the SERCA pump, Ca2+ oscillations, and the translocation of NFAT. The localization of ORP5 and ORP8 at specific subdomains further regulates their function, and the hydrolysis of PtdSer alters the PI(4)P/PtdSer ratio and reproduces the function of ORPs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Gunes Parlakgul, Ana Paula Arruda, Song Pang, Erika Cagampan, Nina Min, Ekin Guney, Grace Yankun Lee, Karen Inouye, Harald F. Hess, C. Shan Xu, Gokhan S. Hotamisligil
Summary: This study reveals the complex structural organization of organelles in liver tissue and highlights the significant alterations in obese mice. The functional importance of these structural changes on cellular and systemic metabolism is also demonstrated.
Article
Multidisciplinary Sciences
Nathan R. Zaccai, Zuzana Kadlecova, Veronica Kane Dickson, Kseniya Korobchevskaya, Jan Kamenicky, Oleksiy Kovtun, Perunthottathu K. Umasankar, Antoni G. Wrobel, Jonathan G. G. Kaufman, Sally R. Gray, Kun Qu, Philip R. Evans, Marco Fritzsche, Filip Sroubek, Stefan Hoening, John A. G. Briggs, Bernard T. Kelly, David J. Owen, Linton M. Traub
Summary: Clathrin-mediated endocytosis (CME) is a key mechanism for controlling the cell surface proteome in mammalian cells. This study shows that FCHO plays a crucial role in this process by marking the initiation sites of clathrin-coated pits (CCPs) and interacting with other proteins to drive the growth and maturation of CCPs.
Article
Multidisciplinary Sciences
Elizabeth M. Davies, Rajendra Gurung, Kai Qin Le, Katherine T. T. Roan, Richard P. Harvey, Geraldine M. Mitchell, Quenten Schwarz, Christina A. Mitchell
Summary: Dynamic positioning of endothelial tip and stalk cells is essential for angiogenesis, which is regulated by the interplay between VEGFR2 and NOTCH signaling. PI(4,5)P2 hydrolysis by INPP5K contributes to angiogenesis by regulating tip cell specification and DLL4/NOTCH signaling. The inhibition of INPP5K increases PI(4,5)P2 levels, leading to the release of II-catenin from the plasma membrane and activation of AKT signaling, which enhances DLL4/NOTCH transcription.
Review
Biochemistry & Molecular Biology
Yi Wen, Volker M. Vogt, Gerald W. Feigenson
Summary: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) is a crucial component located at the inner leaflet of the cell membrane, constituting only 1-2% of total membrane lipids. Its synthesis and turnover are spatially and temporally regulated. Various factors, such as multivalent cations and cellular proteins interacting with PI(4,5)P-2, contribute to its versatile and dynamic distribution within membranes.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 90, 2021
(2021)
Review
Biochemistry & Molecular Biology
Stefano Perni
Summary: Contraction of striated muscle is triggered by the release of calcium from the sarcoplasmic reticulum into the cytoplasm. This release is initiated by membrane depolarization, which is sensed by voltage-gated calcium channels. Junctophilin1 and Junctophilin2 play a crucial role in forming and stabilizing the junctions between the sarcoplasmic reticulum and the plasma membrane.
Review
Endocrinology & Metabolism
Stanko S. S. Stojilkovic, Tamas Balla
Summary: Plasma membrane and organelle membranes contain phosphoinositides that play important roles in cellular signaling and exocytosis. Three phosphoinositides, PI4P, PI(4,5)P2, and PI(3,4,5)P3, are particularly involved in hormone-producing pituitary cells. PI(4,5)P2 controls various pituitary cell functions, while also serving as a substrate for PI3-kinases to generate intracellular messengers PI(3,4,5)P3 and PI(3,4)P2. PI(4,5)P2 affects pituitary hormone release through ion channel gating, calcium signaling, and the exocytic pathway. PI4P is not only a precursor of PI(4,5)P2 but also a signaling molecule that regulates hormone secretion independently of PI(4,5)P2 in various cell types, including pituitary cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Xinan Meng, Chandra Sugiarto Wijaya, Qingfang Shao, Suhong Xu
Summary: The Golgi apparatus accumulates at the wound site and facilitates membrane repair by converting PtdIns4P to PtdIns(4,5)P2 in response to plasma membrane damage in C. elegans. This finding advances our understanding of cellular survival mechanisms under mechanical stress.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maria J. Sarmento, Luis Borges-Araujo, Sandra N. Pinto, Nuno Bernardes, Joana C. Ricardo, Ana Coutinho, Manuel Prieto, Fabio Fernandes
Summary: PI(4,5)P-2 plays essential roles in cellular functions by dynamically organizing within the plasma membrane; in HeLa cells, it is compartmentalized without cholesterol dependence, and actin polymerization inhibition eliminates the compartmentalization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Douglas M. Anderson, Kelly M. Anderson, Chi-Lun Chang, Catherine A. Makarewich, Benjamin R. Nelson, John R. McAnally, Prasad Kasaragod, John M. Shelton, Jen Liou, Rhonda Bassel-Duby, Eric N. Olson
Editorial Material
Cell Biology
Jen Liou, Chi-Lun Chang
Article
Biochemistry & Molecular Biology
Chi-Lun Chang, Jen Liou
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Review
Biochemistry & Molecular Biology
Chi-Lun Chang, Jen Liou
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2016)
Article
Cell Biology
Ting-Sung Hsieh, Yu-Ju Chen, Chi-Lun Chang, Wan-Ru Lee, Jen Liou
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Cell Biology
Chi-Lun Chang, Yu-Ju Chen, Carlo Giovanni Quintanilla, Ting-Sung Hsieh, Jen Liou
JOURNAL OF CELL BIOLOGY
(2018)
Article
Cell Biology
Yuan-Li Huang, Chi-Lun Chang, Chih-Hsin Tang, Yueh-Chien Lin, Tsai-Kai Ju, Wei-Pang Huang, Hsinyu Lee
CELLULAR SIGNALLING
(2014)
Article
Cell Biology
Chi-Lun Chang, Ting-Sung Hsieh, T. Tony Yang, Karen G. Rothberg, D. Berfin Azizoglu, Elzibeth Volk, Jung-Chi Liao, Jen Liou
Article
Biochemistry & Molecular Biology
Maria S. Ioannou, Jesse Jackson, Shu-Hsien Sheu, Chi-Lun Chang, Aubrey V. Weigel, Hui Liu, H. Amalia Pasolli, C. Shan Xu, Song Pang, Doreen Matthies, Harald F. Hess, Jennifer Lippincott-Schwartz, Zhe Liu
Article
Cell Biology
Chi-Lun Chang, Aubrey Weigel, Maria S. Ioannou, H. Amalia Pasolli, C. Shan Xu, David R. Peale, Gleb Shtengel, Melanie Freeman, Harald F. Hess, Craig Blackstone, Jennifer Lippincott-Schwartz
JOURNAL OF CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
David P. Hoffman, Gleb Shtengel, C. Shan Xu, Kirby R. Campbell, Melanie Freeman, Lei Wang, Daniel E. Milkie, H. Amalia Pasolli, Nirmala Iyer, John A. Bogovic, Daniel R. Stabley, Abbas Shirinifard, Song Pang, David Peale, Kathy Schaefer, Wim Pomp, Chi-Lun Chang, Jennifer Lippincott-Schwartz, Tom Kirchhausen, David J. Solecki, Eric Betzig, Harald F. Hess
Article
Biochemistry & Molecular Biology
Aubrey Weigel, Chi-Lun Chang, Gleb Shtengel, C. Shan Xu, David P. Hoffman, Melanie Freeman, Nirmala Iyer, Jesse Aaron, Satya Khuon, John Bogovic, Wei Qiu, Harald F. Hess, Jennifer Lippincott-Schwartz
Summary: Cellular versatility relies on accurate trafficking of proteins to their organelles, particularly through the secretory pathway which involves an elaborate network of interconnected tubules originating from the endoplasmic reticulum. COPII protein regulates cargo entry at the ER neck region, while COPI protein escorts tubular entities towards the Golgi apparatus via microtubule-directed movement.
Review
Cell Biology
Isha Ralhan, Chi-Lun Chang, Jennifer Lippincott-Schwartz, Maria S. Ioannou
Summary: Lipid droplets are dynamic organelles in cells that store lipids and respond to the cellular physiological state, providing protection against stressors like oxidative stress. Recent research has started to uncover the role of lipid droplets in brain cells, particularly in the nervous system, with descriptions now available regarding their functions in development, aging, and various neuropathologies.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Chi-Lun Chang, Yu-Ju Chen, Jen Lou
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2017)