Review
Biotechnology & Applied Microbiology
Paloma Fuentes, Maria Jose Torres, Rodrigo Arancibia, Francisco Aulestia, Mauricio Vergara, Flavio Carrion, Nelson Osses, Claudia Altamirano
Summary: Conditioned medium (CM) obtained from the culture of mesenchymal stromal/stem cells (MSCs) has been shown to effectively promote tissue repair and modulate the immune response, offering advantages over direct MSC implantation. Research has focused on characterizing CM components and optimizing preconditioning strategies to enhance therapeutic potential. Dynamic cultivation conditions, like 3D culture, have been shown to impact MSC behavior and increase growth factor production for potential CM applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Health Care Sciences & Services
Alisa Domnina, Larisa Alekseenko, Irina Kozhukharova, Olga Lyublinskaya, Mariia Shorokhova, Valeriy Zenin, Irina Fridlyanskaya, Nikolay Nikolsky
Summary: Formation of multicellular aggregates known as spheroids from eMSCs enhances tissue repair capabilities, showing higher therapeutic potency compared to monolayer eMSC culture.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Anatomy & Morphology
Alessia Paganelli, Luisa Benassi, Elena Rossi, Elisabetta Tarentini, Cristina Magnoni
Summary: Adipose derived mesenchymal stromal cells (ADSCs) play a significant role in wound healing and regenerative medicine, as they stimulate epithelial cells through secretion of soluble factors and promote re-epithelization by proliferating basal stem cells.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Engineering, Biomedical
Jay M. McKinney, Krishna A. Pucha, Thanh N. Doan, Lanfang Wang, Laura D. Weinstock, Benjamin T. Tignor, Kelsey L. Fowle, Rebecca D. Levit, Levi B. Wood, Nick J. Willett
Summary: The study evaluated the effects of sodium alginate microencapsulation on human MSCs in treating OA, and found that encapsulated MSCs can reduce cartilage degeneration and bone remodeling. In response to IL-1 beta stimulation, encapsulated MSCs showed a targeted secretory response, while nonencapsulated MSCs showed an indiscriminate increase in cytokines.
ACTA BIOMATERIALIA
(2022)
Review
Cell Biology
Mohamad Mahjoor, Arshia Fakouri, Simin Farokhi, Hojjatollah Nazari, Hamed Afkhami, Fatemeh Heidari
Summary: The skin is essential for the innate and adaptive immune systems, but skin lesions can have undesirable consequences. Mesenchymal stromal cells (MSCs) are being increasingly used for skin wound treatment due to their advantages in promoting healing. The oxygen concentration has an impact on MSC biology, and expanding MSCs in a hypoxic environment can enhance their effectiveness.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Medicine, General & Internal
Jia-Yi Ding, Min-Jiang Chen, Ling-Feng Wu, Gao-Feng Shu, Shi-Ji Fang, Zhao-Yu Li, Xu-Ran Chu, Xiao-Kun Li, Zhou-Guang Wang, Jian-Song Ji
Summary: Skin wounds result from trauma, tearing, cuts, or contusions, and can impose a significant socioeconomic burden. Recent studies have focused on the use of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) for wound repair. MSC-derived EVs offer a more stable and safer alternative to cell therapy, and can promote the repair of damaged substructures and inhibit scar formation. Furthermore, they can be used as a scaffold and developed into bioengineered EVs for trauma repair. Understanding the detailed mechanism of EVs will allow for their effective use in clinical treatments for wound repair.
MILITARY MEDICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Alessia Gallo, Nicola Cuscino, Flavia Contino, Matteo Bulati, Mariangela Pampalone, Giandomenico Amico, Giovanni Zito, Claudia Carcione, Claudio Centi, Alessandro Bertani, Pier Giulio Conaldi, Vitale Miceli
Summary: Studies have shown that MSC spheroids cultured in three dimensions exhibit enhanced therapeutic properties and can regulate processes such as proliferation/differentiation, immunomodulation, and angiogenesis. This represents a promising approach to cell-based therapy and has significant implications for regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Seungwon An, Khandaker Anwar, Mohammadjavad Ashraf, Hyungjo Lee, Rebecca Jung, Raghuram Koganti, Mahmood Ghassemi, Ali R. Djalilian
Summary: In this study, the researchers investigated the use of human bone-marrow-derived MSC secretome (MSC-S) for treating corneal epithelial wounds. They found that MSC extracellular vesicles (EV)/exosomes played a crucial role in mediating the wound-healing effects of MSC-S. Furthermore, they discovered that the concentration and incubation period of MSC-CM had an impact on wound healing.
Article
Oncology
Zhenbo Tu, Antoine E. Karnoub
Summary: Mesenchymal stem/stromal cells (MSCs) are a heterogeneous population of fibroblastic progenitor cells that reside in multiple tissues around the body and have the ability to differentiate into various connective tissue cells. They play critical regulatory roles in breast tumor development and progression, participating in multiple aspects of the process.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Medicine, General & Internal
Hyun-Jee Kim, Sungho Shin, Seon-Yeong Jeong, Sun-Ung Lim, Dae-Won Lee, Yunhee-Kim Kwon, Jiyeon Kang, Sung-Won Kim, Chan-Kwon Jung, Cheolju Lee, Il-Hoan Oh
Summary: TB-MSCs derived from the turbinate exhibit unique characteristics compared to BM-MSCs, showing higher neural crest-like properties and a distinct secretome profile. While TB-MSCs have potential for neurogenic differentiation, they also display comparable immunosuppressive effects as BM-MSCs, but with a stronger stimulatory effect on breast cancer cell growth and metastasis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Rui Alvites, Mariana Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Ana Colette Mauricio
Summary: Mesenchymal Stem/Stromal Cells (MSCs) have high clinical value and have been extensively studied. Their immunomodulatory influence occurs through both direct and paracrine routes, but the mechanisms are not fully understood. This review summarizes the immunoregulatory capacity of MSCs and their secretion products, with a focus on migration, homing, direct cell action, and paracrine activity, and explores related techniques.
Review
Cell Biology
Raquel Guillamat-Prats
Summary: This review discusses the effects of MSCs and their derived products in each step of the wound repair process, as well as the pre-clinical and clinical use of MSCs in skin wound healing in diabetic associated wounds and in pathophysiological fibrosis.
Article
Biochemistry & Molecular Biology
Elia Bari, Fulvio Tartara, Fabio Cofano, Giuseppe di Perna, Diego Garbossa, Sara Perteghella, Marzio Sorlini, Delia Mandracchia, Lorella Giovannelli, Paolo Gaetani, Maria Luisa Torre, Lorena Segale
Summary: The study found that the mesenchymal stem cell-secretome Lyosecretome has a significant improvement effect on the osteoinductive and osteoconductive properties of titanium cages, leading to increased mineralized matrix when used during culturing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Elia Bari, Ilaria Roato, Giuseppe Perale, Filippo Rossi, Tullio Genova, Federico Mussano, Riccardo Ferracini, Marzio Sorlini, Maria Luisa Torre, Sara Perteghella
Summary: A new strategy was developed to improve the osteoinductivity of SmartBone (SB) by loading the SB scaffolds with freeze-dried formulation of mesenchymal stem cell (MSC)-secretome. The study showed that SBlyo significantly enhanced cell proliferation and tissue formation compared to SB, suggesting a more effective bone formation induction by SBlyo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Jiujiang Zeng, Xi Chen, Jiahui Zhang, Yechi Qin, Kunxi Zhang, Xinping Li, Haiyan Cui
Summary: By developing a PNIPAM-based porous hydrogel, numerous ASC spheroids can be efficiently prepared and applied to skin wound healing, promoting tissue regeneration and cell therapy effect.
MATERIALS TODAY ADVANCES
(2022)
Review
Orthopedics
Pedro Diniz, Jacome Pacheco, Ricardo M. Fernandes, Helder Pereira, Frederico Castelo Ferreira, Gino M. M. J. Kerkhoffs
Summary: This study compared failure load and elongation after cyclic loading of different Achilles tendon repair techniques, and found that certain repair techniques ranked higher in these measures.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Orthopedics
Pedro Diniz, Carlos Quental, Pedro Violindo, Joao Veiga Gomes, Helder Pereira, Gino M. M. J. Kerkhoffs, Frederico Castelo Ferreira, Joao Folgado
Summary: This study developed a three-dimensional finite element model of the Achilles tendon that includes subtendon twisting and sliding. The model was validated using in vivo experimental data and showed sensitivity to subtendon twist and dispersion of collagen fiber orientations.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Orthopedics
Pedro Diniz, Andre Soares Ferreira, Ligia Figueiredo, Jorge Pablo Batista, Nasef Abdelatif, Helder Pereira, Gino M. M. J. Kerkhoffs, Stan N. Finkelstein, Frederico Castelo Ferreira
Summary: This study performed an early cost-effectiveness analysis of endoscopic flexor hallucis longus (FHL) transfer for acute Achilles tendon ruptures and found that the surgical treatment has a moderate likelihood of being cost-effective, with endoscopic FHL transfer showing the highest potential.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Nuno Torres Faria, Miguel Figueiredo Nascimento, Flavio Alves Ferreira, Teresa Esteves, Marisa Viegas Santos, Frederico Castelo Ferreira
Summary: Biosurfactants are environmentally friendly alternatives to fossil-driven surfactants due to their low toxicity and high biodegradability. However, their large-scale production and application are hindered by high costs. This study proposes a novel strategy for cost-effective MEL production by combining different carbon sources and optimizing downstream processing using nanofiltration technology.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ana Fernandes-Platzgummer, Raquel Cunha, Sara Morini, Marta Carvalho, Juan Moreno-Cid, Carmen Garcia, Joaquim M. S. Cabral, Claudia L. da Silva
Summary: This study successfully establishes a serum-/xenogeneic-free microcarrier-based culture system for the cultivation of bone marrow-derived MSC and production of MSC-EVs. Large numbers of cells and EVs were obtained through static culture and continuous perfusion. The size and concentration of EVs were estimated using nanoparticle tracking analysis.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Engineering, Industrial
Boyuan Li, Changjun Han, Yuxi Xie, Paulo Jorge Da Silva Bartolo, Kun Zhou
Summary: This work investigated the effect of thermal history on the microstructure and mechanical properties of a DED-printed nickel-aluminum-bronze part for the first time. Significant microstructural and mechanical heterogeneity was observed at the top, middle, and bottom locations of the printed part. The bottom location exhibited the highest mechanical strengths.
CIRP ANNALS-MANUFACTURING TECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mariana A. Branco, Tiago C. Nunes, Joaquim M. S. Cabral, Maria Margarida Diogo
Summary: It is now acknowledged that drug use during pregnancy is necessary to prevent or treat illnesses, but there is often a lack of information on teratogenic risk in humans. Animal models have been the main source of data, but their limitations in predicting human outcomes have led to misidentification of teratogenicity. The development of humanized in vitro models, particularly those that replicate important early developmental stages, can overcome these limitations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Pedro Marcelino, Joao Carlos Silva, Carla S. Moura, Joao Meneses, Rachel Cordeiro, Nuno Alves, Paula Pascoal-Faria, Frederico Castelo Ferreira
Summary: Osteochondral (OC) defects affect both articular cartilage and the underlying subchondral bone, and current therapies have not yet found a suitable long-term solution. Tissue engineering (TE) strategies aim to fabricate tissue substitutes that recreate natural tissue features. This study presents a novel method for designing scaffolds with a mathematically defined curvature, based on the geometry of a sphere, to obtain TE constructs mimicking native OC tissue shape.
Article
Polymer Science
Fabio F. F. Garrudo, Giulia Filippone, Leonor Resina, Joao C. Silva, Frederico Barbosa, Luis F. V. Ferreira, Teresa Esteves, Ana Clara Marques, Jorge Morgado, Frederico Castelo Ferreira
Summary: This study successfully incorporated poly(ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) into Poly(acrylonitrile) (PAN) fibers for the first time. PAN was chosen as a carrier due to its biocompatibility and tunable chemical stability. The resulting PAN:PEDOT:PSS fibers showed enhanced electroconductivity and biocompatibility, making them suitable for neural tissue engineering and soft electronics.
Review
Polymer Science
Fabio F. F. Garrudo, Robert J. Linhardt, Frederico Castelo Ferreira, Jorge Morgado
Summary: Electrical stimulation is a powerful strategy for enhancing neural stem cell differentiation into neurons for the treatment of neurological diseases. Poly(aniline):camphorsulfonic acid (PANI:CSA) is a commonly studied electroconductive polymer that can direct electrical fields to neural cells in culture. This review examines the current literature on electrical stimulation of neural cells, specifically focusing on the fundamentals of bioelectricity, the use of PANI:CSA-based systems, and the development of scaffolds and setups for electrical stimulation.
Article
Biochemistry & Molecular Biology
Claudia Raquel Marques, Miguel de Almeida Fuzeta, Raquel Medina dos Santos Cunha, Joana Pereira-Sousa, Deolinda Silva, Jonas Campos, Andreia Teixeira-Castro, Rui Amandi Sousa, Ana Fernandes-Platzgummer, Claudia L. da Silva, Antonio Jose Salgado
Summary: Parkinson's disease is a neurodegenerative disorder characterized by the degeneration of dopamine neurons in the substantia nigra pars compacta. The presence of alpha-synuclein aggregates is considered a main contributor to the disease. Mesenchymal stromal cells have the potential to be used as a cell-free therapy for Parkinson's disease. However, there is a need to develop a protocol for large-scale production of the secretome under good manufacturing practices guidelines.
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Biochemistry & Molecular Biology
Frederico Barbosa, Fabio F. F. Garrudo, Ana C. Marques, Joaquim M. S. Cabral, Jorge Morgado, Frederico Castelo Ferreira, Joao C. Silva
Summary: This study aimed to develop electroconductive PAN/PEDOT:PSS nanofibers via electrospinning, which can mimic the fibrous extracellular matrix (ECM) of natural tissue and provide a platform for the delivery of exogenous electrical stimulation (ES). The resulting nanofibers were functionalized with apatite-like structures to imitate the inorganic phase of the bone ECM. The mineralized electroconductive nanofibers demonstrated improved biological performance, including enhanced proliferation of cells and up-regulated expression levels of bone marker genes, making them potential electroactive biomimetic scaffolds for bone defect repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)