Article
Biophysics
Ricardo P. Martinho, Mukul G. Jain, Lucio Frydman
Summary: This study explores the use of two-dimensional NMR techniques to tackle the challenges of metabolic resonances in the brain. By comparing conventional and double quantum-filtered correlated spectroscopy traces, the study demonstrates the improved assignment of in vivo resonances with the aid of more sensitive ex vivo 2D acquisitions. The enhanced resolution allows for the identification of approximately 29 metabolites and provides insights into metabolite compartmentalization.
NMR IN BIOMEDICINE
(2023)
Article
Polymer Science
Abolfazl Heydari, Eva Dusicka, Matej Micusik, Marian Sedlak, Igor Lacik
Summary: In this study, the characteristics of N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan salt (HTCS) were investigated, revealing a significant exchange of chloride counterions during synthesis, with the true degree of quaternization determined by NMR spectroscopy. This phenomenon of counterion exchange was further validated by XPS and shown for quaternized dextran, highlighting the importance of considering counterion type in applications of polyelectrolytes.
Article
Multidisciplinary Sciences
Joydeep Dutta, Priyanka
Summary: In this study, a novel acid-base titration method was proposed for the determination of the degree of deacetylation (DD) of chitosan, which showed good agreement with reported values. This simple and reliable method has significant implications for research on chitosan.
Review
Biochemistry & Molecular Biology
Elizabeth A. Komives
Summary: Thrombin is a versatile protein enzyme that plays multiple roles in blood coagulation, cell signaling, and anticoagulation. It has an allosteric coupling between its active site and anion binding exosite 1. In this review, various techniques including mass spectrometry and NMR spectroscopy were used to study the structural changes and dynamics of thrombin upon effector binding or mutagenesis, and computational network analysis was employed to analyze the thrombin-thrombomodulin complex.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Yinping Wang, Yannick Coppel, Christine Lepetit, Jean-Daniel Marty, Christophe Mingotaud, Myrtil L. Kahn
Summary: This study elucidates the crucial role of amine surfactants in the controlled anisotropic growth of ZnO nanoparticles and their impact on the nature of molecular species in solution, as well as the dynamic behavior on the surface of the grown ZnO nanocrystals. The structuring influence of alkyl substituents on the nitrogen atom of amines strongly influences the formation of molecular species in the solution and the subsequent growth of ZnO nanocrystals. Surface dynamic behavior, particularly H-bonding, plays a predominant role in driving nanocrystal growth and morphology determination, highlighting the importance of weak surface interactions in nanoscience progress.
NANOSCALE ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Jaka Kragelj, Thibault Orand, Elise Delaforge, Laura Tengo, Martin Blackledge, Andres Palencia, Malene Ringkjobing Jensen
Summary: Intrinsically disordered proteins (IDPs) can form promiscuous interactions with their protein targets, facilitated by folding-upon-binding into different conformations. Specific surface properties of the kinases p38 alpha and JNK1 dictate the bound conformation of the regulatory domain MKK4, with enthalpy-entropy compensation playing a major role in maintaining comparable binding affinities towards the two kinases.
Article
Chemistry, Multidisciplinary
Takenori Dairaku, Kyoko Furuita, Hajime Sato, Jakub Sebera, Daichi Yamanaka, Hiroyuki Otaki, Shoko Kikkawa, Yoshinori Kondo, Ritsuko Katahira, F. Matthias Bickelhaupt, Celia Fonseca Guerra, Akira Ono, Vladimir Sychrovsky, Chojiro Kojima, Yoshiyuki Tanaka
CHEMICAL COMMUNICATIONS
(2015)
Article
Biochemistry & Molecular Biology
Takenori Dairaku, Kyoko Furuita, Hajime Sato, Yoshinori Kondo, Chojiro Kojima, Akira Ono, Yoshiyuki Tanaka
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2015)
Article
Biochemistry & Molecular Biology
Nesreen Alsanousi, Toshihiko Sugiki, Kyoko Furuita, Masatomo So, Young-Ho Lee, Toshimichi Fujiwara, Chojiro Kojima
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Chemistry, Multidisciplinary
Takenori Dairaku, Kyoko Furuita, Hajime Sato, Jakub Sebera, Katsuyuki Nakashima, Jiro Kondo, Daichi Yamanaka, Yoshinori Kondo, Itaru Okamoto, Akira Ono, Vladimir Sychrovsky, Chojiro Kojima, Yoshiyuki Tanaka
CHEMISTRY-A EUROPEAN JOURNAL
(2016)
Article
Biology
Hisashi Tatebe, Shinichi Murayama, Toshiya Yonekura, Tomoyuki Hatano, David Richter, Tomomi Furuya, Saokri Kataoka, Kyoko Furuita, Chojiro Kojima, Kazuhiro Shiozaki
Article
Biochemistry & Molecular Biology
Toshihiko Sugiki, Kyoko Furuita, Toshimichi Fujiwara, Chojiro Kojima
Review
Biochemistry & Molecular Biology
Toshihiko Sugiki, Kyoko Furuita, Toshimichi Fujiwara, Chojiro Kojima
Article
Biophysics
Saori Kataoka, Kyoko Furuita, Yoshikazu Hattori, Naohiro Kobayashi, Takahisa Ikegami, Kazuhiro Shiozaki, Toshimichi Fujiwara, Chojiro Kojima
BIOMOLECULAR NMR ASSIGNMENTS
(2015)
Article
Biochemistry & Molecular Biology
Ken-ichi Kosami, Izuru Ohki, Minoru Nagano, Kyoko Furuita, Toshihiko Sugiki, Yoji Kawano, Tsutomu Kawasaki, Toshimichi Fujiwara, Atsushi Nakagawa, Ko Shimamoto, Chojiro Kojima
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Yoshikazu Hattori, Kyoko Furuita, Izuru Ohki, Takahisa Ikegami, Harumi Fukada, Masahiro Shirakawa, Toshimichi Fujiwara, Chojiro Kojima
JOURNAL OF BIOMOLECULAR NMR
(2013)
Article
Biochemistry & Molecular Biology
Kyoko Furuita, Saori Kataoka, Toshihiko Sugiki, Yoshikazu Hattori, Naohiro Kobayashi, Takahisa Ikegami, Kazuhiro Shiozaki, Toshimichi Fujiwara, Chojiro Kojima
JOURNAL OF BIOMOLECULAR NMR
(2015)
Article
Chemistry, Multidisciplinary
Kyoko Furuita, Shunpei Murata, Jun Goo Jee, Satoshi Ichikawa, Akira Matsuda, Chojiro Kojima
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2011)
Article
Biochemical Research Methods
Kyoko Furuita, Toshihiko Sugiki, Mika Takamuku, Yoshikazu Hattori, Masatomo So, Yasushi Kawata, Takahisa Ikegami, Toshimichi Fujiwara, Chojiro Kojima
Summary: This study aimed to enhance the sensitivity of C-13-direct detection experiments by developing new pulse sequences and virtual decoupling procedures. The results showed that the developed HCACO-type pulse sequences successfully increased the sensitivity of the C-13-direct detection experiment, which has the potential to extend its utility, especially for structural analyses of intrinsically disordered proteins.
JOURNAL OF MAGNETIC RESONANCE
(2021)
Article
Biochemistry & Molecular Biology
Kyoko Furuita, Marina Hiraoka, Kentaro Hanada, Toshimichi Fujiwara, Chojiro Kojima
Summary: The study found that the major sperm protein domain of VAP binds specifically to peptides containing FFAT-like motifs, contributing to the discovery of new VAP interactors. Additionally, the RNA-dependent RNA polymerase of SARS-CoV-2 also contains an FFAT-like motif that binds specifically to VAP-A(MSP) and other FFAT-like motifs.