Article
Materials Science, Multidisciplinary
Georgios Chondrogiannis, Pedro Reu, Mahiar M. Hamedi
Summary: Nucleic acid amplification testing (NAAT) is the gold standard for infectious disease diagnostics, but it is mainly limited to centralized laboratories. Researchers have addressed the bottleneck issue of DNA extraction by immobilizing the enzyme ACP on nitrocellulose, enabling home-based kits and expanding the capabilities of home-testing beyond antigen tests.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Yile Fang, Yue Wang, Liangxi Zhu, Haoran Liu, Xiangyi Su, Yuan Liu, Zhu Chen, Hui Chen, Nongyue He
Summary: In this paper, a self-contained cartridge with a simple structure was developed for point-of-care detection of infectious diseases. The cartridge utilizes magnetic nanoparticles for nucleic acid extraction and automated detection process. The performance tests showed that it met the demands of automated nucleic acid detection, and it successfully detected hepatitis B virus and severe acute respiratory syndrome coronavirus 2.
CHINESE CHEMICAL LETTERS
(2023)
Review
Chemistry, Multidisciplinary
Yuyue Xu, Tao Wang, Zhu Chen, Lian Jin, Zuozhong Wu, Jinqu Yan, Xiaoni Zhao, Lei Cai, Yan Deng, Yuan Guo, Song Li, Nongyue He
Summary: Point-of-care nucleic acid testing (POCNAT) plays a crucial role in infectious disease outbreaks, offering rapid, simple, and automated detection of nucleic acid. Leveraging advancements in technology, novel experimental carriers like chips, cartridges, and papers have been employed for automated nucleic acid detection, marking significant progress in the field.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Rui Wang, Jing Wu, Xiaodong He, Peng Zhou, Zuojun Shen
Summary: This research presents an automated microfluidic system that integrates the functions of a traditional PCR laboratory into a palm-sized microfluidic card, capable of typing 24 HPV genotypes. The system achieves a detection limit of 200 copies/test and a 100% positive detection rate for clinical samples, making it an effective method for HPV detection.
Review
Biotechnology & Applied Microbiology
Jingwen Wang, Han Jiang, Leiming Pan, Xiuying Gu, Chaogeng Xiao, Pengpeng Liu, Yulong Tang, Jiehong Fang, Xiaoqian Li, Chenze Lu
Summary: As nucleic acid testing becomes crucial in various research fields, there is an increasing demand for rapid on-site testing methods. This review discusses recent advances in on-chip methods for sample preparation, amplification, and detection in the nucleic acid testing procedure. It covers integration strategies for sample preparation, various on-chip amplification methods, and evaluation of potential detection methods. The review also highlights the importance of improving the speed and integration of all steps onto a single chip for future developments in nucleic acid testing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Yugan He, Tie Xie, Qihang Tu, Yigang Tong
Summary: Nucleic acid testing is widely used for detecting SARS-CoV-2, but it may yield false-negative results for samples with low viral loads. This study used a Poisson distribution to demonstrate the inconsistent performance of nucleic acid testing in detecting low viral load samples. The researchers found that increasing the sample input volume improved the consistency, accuracy, and robustness of the nucleic acid testing for SARS-CoV-2 samples with low viral loads.
ANALYTICA CHIMICA ACTA
(2022)
Review
Biochemistry & Molecular Biology
Ousama Al Shanaa, Andrey Rumyantsev, Elena Sambuk, Marina Padkina
Summary: This review discusses the early stages, main developments, and latest technologies of RNA aptamer research, emphasizing the advantages of RNA aptamers compared to antibodies and their potential applications in the near future.
Article
Biophysics
Hui Wu, Yanju Chen, Ya Shi, Liu Wang, Mengyao Zhang, Jian Wu, Huan Chen
Summary: The polypropylene (PP) bag-based method for nucleic acid detection offers the potential for at-home testing, with the ability to visually detect Salmonella typhimurium (St) and SARS-CoV-2 within 1 hour; the combination of magnetic particles and isothermal amplification technology allows for convenient processing. Introducing a logic decision method enhances the reliability of at-home nucleic acid testing.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Le Tran Huy Thang, Won Han, Jaeho Shin, Joong Ho Shin
Summary: In this study, a pressure-driven and self-contained cartridge with pre-stored reagents was designed to automate nucleic acid purification. By using pressurized air stored inside the cartridge, reagents were transferred through a microfluidic chip, which reduced operational complexity. The device achieved similar results to the conventional column-based extraction method in terms of concentration, purity, and real-time polymerase chain reaction (qPCR) for Escherichia coli (E. coli) O157:H7 DNA. It also had a detection limit of 103 CFU, similar to the conventional solid-phase extraction method. This novel approach for automating sample preparation can facilitate point-of-care molecular diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Hong Duan, Wuzhen Qi, Siyuan Wang, Lingyan Zheng, Jing Yuan, Jianhan Lin
Summary: In this study, a new colorimetric immunoassay was proposed for the rapid and sensitive screening of pathogens. The assay utilized the release of Ag+ ions from AgNPs and the inhibition of PtNPs. A microfluidic platform, controlled by Raspberry Pi and a smartphone app, was also developed for automated bacterial detection. The immunoassay showed high specificity, an ultralow detection limit, and good recovery for spiked chicken samples, showcasing its potential for ensuring food safety.
ANALYTICA CHIMICA ACTA
(2022)
Article
Energy & Fuels
Bryn Pickering, Ruchi Choudhary
Summary: This paper explores a novel workflow to quantitatively measure increases in system resilience, demonstrating through a case study in Bangalore that scenario optimization can significantly improve system resilience. It highlights the inability of systems designed for demand uncertainty to handle extreme shocks from interruptions, as well as the importance of reducing grid dependence to address shock-induced instability. Additionally, a new approach balancing cost, CO2 emissions, and system resilience is identified through out-of-sample testing, emphasizing the necessity for such testing in energy system modeling.
Review
Chemistry, Analytical
Soo Min Lee, Hari Kalathil Balakrishnan, Egan H. Doeven, Dan Yuan, Rosanne M. Guijt
Summary: Nucleic acid amplification testing is an effective method for disease detection and is suitable for point-of-need testing. Sample preparation techniques are crucial for maximizing the sensitivity and reliability of testing. This review focuses on the reagents used in sample preparation for nucleic acid testing and their suitability for use in a portable format.
Review
Food Science & Technology
Tai-Yong Kim, Xiaoning Zhu, Se-Min Kim, Jeong-A Lim, Min-Cheol Lim, Ke Luo
Summary: Viruses are major pathogens in food poisoning, and the development of foodborne virus detection technologies for the entire food distribution chain is crucial for food safety. Polymerase chain reaction (PCR) is the gold standard method for monitoring food contamination by viruses, but it is time-consuming and vulnerable to inhibitors in food samples. Optical and electrical readout biosensors based on nucleic acid isothermal amplification technology and nanomaterials have emerged as alternatives, offering portability and on-site operation. However, the complexity of food components and lack of stability studies on residual food components limit the practical application of biosensors as a universal detection method.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Nicole L. Welch, Meilin Zhu, Catherine Hua, Juliane Weller, Marzieh Ezzaty Mirhashemi, Tien G. Nguyen, Sreekar Mantena, Matthew R. Bauer, Bennett M. Shaw, Cheri M. Ackerman, Sri Gowtham Thakku, Megan W. Tse, Jared Kehe, Marie-Martine Uwera, Jacqueline S. Eversley, Derek A. Bielwaski, Graham McGrath, Joseph Braidt, Jeremy Johnson, Felecia Cerrato, Gage K. Moreno, Lydia A. Krasilnikova, Brittany A. Petros, Gabrielle L. Gionet, Ewa King, Richard C. Huard, Samantha K. Jalbert, Michael L. Cleary, Nicholas A. Fitzgerald, Stacey B. Gabriel, Glen R. Gallagher, Sandra C. Smole, Lawrence C. Madoff, Catherine M. Brown, Matthew W. Keller, Malania M. Wilson, Marie K. Kirby, John R. Barnes, Daniel J. Park, Katherine J. Siddle, Christian T. Happi, Deborah T. Hung, Michael Springer, Bronwyn L. MacInnis, Jacob E. Lemieux, Eric Rosenberg, John A. Branda, Paul C. Blainey, Pardis C. Sabeti, Cameron Myhrvold
Summary: A microfluidic CRISPR-based platform called mCARMEN enables multiplexed and high-throughput identification of a wide range of respiratory viruses, including specific SARS-CoV-2 variants. The platform combines cost-effectiveness, diagnostic-grade performance, and a streamlined workflow for clinical use.
Article
Mathematics
De Zhou, Man Zhou
Summary: This study introduces the experience of pooled nucleic acid testing in China, presents two main pooled testing methods used in Wuhan and Qingdao, and proposes an improved pentagram mini-pooled testing method to speed up testing process and reduce costs. The study mathematically examines the principles and applicability conditions of pooled COVID-19 testing, providing a reference for other countries with different infection rates.
