Article
Cell Biology
Chethan K. Krishna, Nadine Schmidt, Bettina G. Tippler, Wolfgang Schliebs, Martin Jung, Konstanze F. Winklhofer, Ralf Erdmann, Vishal C. Kalel
Summary: This study characterized the PEX19 binding sites of PEX11 in Trypanosoma brucei and Saccharomyces cerevisiae. PEX11 contains two binding sites, one at the N-terminus and a second near the first transmembrane domain. These binding sites are essential for maintaining steady-state concentration and efficient targeting to peroxisomes and glycosomes, and mutations result in protein mislocalization to mitochondria.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Katarzyna M. Zientara-Rytter, Shanmuga S. Mahalingam, Jean-Claude Farre, Krypton Carolino, Suresh Subramani
Summary: Pex11, an abundant peroxisomal membrane protein, is imported to peroxisomal membranes via its interaction with Pex19. It contains a Pex19-binding site (Pex19-BS) that is essential for its membrane insertion. Additionally, Pex11 has a Pex19-independent peroxisome-targeting signal (mPTS) that anchors it to the outer leaflet of the peroxisomal membrane. Pex19 acts as a chaperone that protects Pex11 from spontaneous oligomerization and subsequent degradation.
Review
Physiology
Ruth E. Carmichael, Michael Schrader
Summary: Organelle membranes are highly dynamic and exhibit plasticity, impacting the biogenesis and function of organelles. Understanding the dynamics of peroxisomal membranes is crucial for healthy cell function. Multidisciplinary research is needed to unravel the roles and regulation of peroxisome membrane dynamics.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Microbiology
Celine Yen Ling Choo, Pei-Ching Wu, Jonar I. Yago, Kuang-Ren Chung
Summary: In this study, the researchers discovered a microbody called peroxisomes that are involved in fatty acid and hydrogen peroxide metabolism in eukaryotes. They found that the AaPex3 gene is responsible for peroxisome biogenesis and resistance to peroxides and superoxide-generating compounds. AaPex3 also affects gene expression related to ROS resistance and is involved in maintaining cell wall integrity and various important functions in the phytopathogenic fungus.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Medical Laboratory Technology
Irene De Biase, Tatiana Yuzyuk, Wei Cui, Lauren M. Zuromski, Ann B. Moser, Nancy E. Braverman
Summary: Plasmalogens are glycerophospholipids with important roles in cellular processes, and their deficiency is associated with neurodegenerative diseases and peroxisome biogenesis disorders. A novel LC-MS/MS method was developed to quantify phosphoethanolamine plasmalogens in red blood cells, providing a specific and precise diagnostic tool. This method has the potential to enhance our understanding of disease pathogenesis and monitor therapy.
CLINICA CHIMICA ACTA
(2023)
Article
Microbiology
Qianqian Liu, Dongni Li, Na Bai, Yingmei Zhu, Jinkui Yang
Summary: This study characterized the function of the Pex14/17 gene in Arthrobotrys oligospora. The results showed that AoPEX14/17 regulates mycelial growth, conidiation, trap formation, autophagy, endocytosis, catalase activity, stress response to oxidants, lipid metabolism, and reactive oxygen species production. Transcriptome analysis and metabolic profile suggested that AoPex14/17 is involved in multiple cellular processes and the regulation of secondary metabolism.
Article
Cell Biology
Melissa S. Traver, Sarah E. Bradford, Jose Luis Olmos, Zachary J. Wright, Mitchell D. Miller, Weijun Xu, George N. Phillips, Bonnie Bartel
Summary: This study reports the crystal structure of the PEX4-PEX22 peroxin complex from Arabidopsis thaliana. PEX4 is a ubiquitin-conjugating enzyme that ubiquitinates proteins associated with the peroxisomal membrane, while PEX22 anchors PEX4 and facilitates its activity. The structure of the Arabidopsis PEX4-PEX22 complex is similar to its yeast orthologs and reveals the potential role of a mutant residue in peroxisomal dysfunction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Hiren Banerjee, Paul LaPointe, Gary Eitzen, Richard A. Rachubinski
Summary: The study identified Pex3 as the essential regulator of glycosome biogenesis, suggesting that small molecule inhibitors that disrupt Pex3-Pex19 interaction could help accelerate drug development for treating Trypanosoma infections.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Stefan Gaussmann, Mohanraj Gopalswamy, Maike Eberhardt, Maren Reuter, Peijian Zou, Wolfgang Schliebs, Ralf Erdmann, Michael Sattler
Summary: In this study, the membrane interactions of PEX5 and PEX14 NTDs were characterized in vitro using membrane mimicking bicelles and nanodiscs, revealing weak interaction of PEX14 NTD with bicelles and multiple interaction sites of PEX5 NTD involving amphipathic alpha-helical regions. Interestingly, the interaction between PEX5 and PEX14 NTDs was found to be largely unaffected by the presence of the membrane, suggesting that docking of PEX5 to PEX14 at the membrane does not reduce the overall binding affinity between the two proteins in the assembly of the peroxisome translocon.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yuichi Yagita, Yuichi Abe, Yukio Fujiki
Summary: Previously, mammalian PEX16 was believed to be essential for peroxisomal membrane biogenesis and maintenance. However, recent studies found that pex16 mutants in other model organisms still have partially functional peroxisomes. It was also discovered that PEX16 accelerates the process of forming peroxisomal membranes in peroxisome-less cells, independently of its role in mediating peroxisomal targeting of PEX3.
JOURNAL OF CELL SCIENCE
(2022)
Article
Immunology
Omkar Indari, Annu Rani, Budhadev Baral, Suleyman Ergun, Kiran Bala, Srikanth Karnati, Hem Chandra Jha
Summary: Viruses exploit peroxisomal compartment for successful infection. This study investigates the modulation of peroxisomal proteins in EBV transformed cell lines and during acute EBV infection. The results reveal distinct regulation of peroxisomal proteins in EBV infected cells and highlight the importance of investigating the manipulation of the peroxisomal compartment in EBV pathologies.
MICROBIAL PATHOGENESIS
(2023)
Article
Multidisciplinary Sciences
Hong-Bo Guo, Zhi-Fei Zhang, Jia-Qing Wang, Si-Yu Wang, Ji-Kang Yang, Xi-Yao Xing, Xiao-Jian Qi, Xiao-Dan Yu
Summary: This study provides insight into the molecular mechanism of autolysis in the fruiting bodies of Coprinus comatus by analyzing the transcriptome. Changes in metabolic pathways during the transition from mature stage to discolored stage were identified, providing a foundation for future studies on the autolysis of C. comatus fruiting bodies.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Mirco Schilff, Yelena Sargsyan, Julia Hofhuis, Sven Thoms
Summary: The study explores the impact of stop codon context on the treatment strategy for genetic premature termination codon diseases and finds that SCC plays a significant role in readthrough stimulation and drug concentration selection.
Article
Multidisciplinary Sciences
Toshinori Oozeki, Tadashi Nakai, Kazuki Kozakai, Kazuki Okamoto, Shun'ichi Kuroda, Kazuo Kobayashi, Katsuyuki Tanizawa, Toshihide Okajima
Summary: In this study, a flavoprotein monooxygenase essential for the biosynthesis of cysteine tryptophylquinone (CTQ) cofactor is characterized both functionally and structurally. The diversity in tryptophylquinone-generating systems suggests convergent evolution of tryptophan-derived cofactors in various proteins.
NATURE COMMUNICATIONS
(2021)
Review
Physiology
Ronald J. A. Wanders, Myriam Baes, Daniela Ribeiro, Sacha Ferdinandusse, Hans R. Waterham
Summary: Peroxisomes are subcellular organelles that play a crucial role in human physiology by catalyzing unique metabolic functions. They require the interaction with other organelles through tethering proteins to achieve efficient metabolite transfer. This review focuses on the metabolic role of peroxisomes in humans, discussing their partnership with other organelles, consequences of genetic defects, and their underrated role in viral infections.
PHYSIOLOGICAL REVIEWS
(2023)
Article
Microbiology
Zhi-Jie Xia, Hong-Zhen Wu, Chun-Xiao Cui, Qi Chen, Guo-Yan Zhao, Hai-Xia Wang, Mei-Xue Dai
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2016)
Article
Cell Biology
Gaurav Agrawal, Scott N. Fassas, Zhi-Jie Xia, Suresh Subramani
JOURNAL OF CELL BIOLOGY
(2016)
Article
Microbiology
Guo-Yan Zhao, Li-Ya Zhao, Zhi-Jie Xia, Jin-Lei Zhu, Di Liu, Chun-Yue Liu, Xiu-Lan Chen, Yu-Zhong Zhang, Xi-Ying Zhang, Mei-Xue Dai
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2017)
Article
Cell Biology
Wei Wang, Zhi-Jie Xia, Jean-Claude Farre, Suresh Subramani
JOURNAL OF CELL BIOLOGY
(2017)
Article
Cell Biology
Wei Wang, Zhijie Xia, Jean-Claude Farre, Suresh Subramani
Article
Microbiology
Peng-Yi Zhang, Pei-Pei Xu, Zhi-Jie Xia, Jing Wang, Juan Xiong, Yue-Zhong Li
FEMS MICROBIOLOGY LETTERS
(2013)
Article
Multidisciplinary Sciences
Ying Chen, Shiheng Liu, Cuilan Liu, Yan Huang, Kaikai Chi, Tiantian Su, Deyu Zhu, Jin Peng, Zhijie Xia, Jing He, Sujuan Xu, Wei Hu, Lichuan Gu
SCIENTIFIC REPORTS
(2016)
Article
Genetics & Heredity
Carlos R. Ferreira, Zhi-Jie Xia, Aurelie Clement, David A. Parry, Mariska Davids, Fulya Taylan, Prashant Sharma, Coleman T. Turgeon, Bernardo Blanco-Sanchez, Bobby G. Ng, Clare V. Logan, Lynne A. Wolfe, Benjamin D. Solomon, Megan T. Cho, Ganka Douglas, Daniel R. Carvalho, Heiko Bratke, Marte Gjol Haug, Jennifer B. Phillips, Jeremy Wegner, Michael Tiemeyer, Kazuhiro Aoki, Ann Nordgren, Anna Hammarsjo, Angela L. Duker, Luis Rohena, Hanne Buciek Hove, Jakob Ek, David Adams, Cynthia J. Tifft, Tito Onyekweli, Tara Weixel, Ellen Macnamara, Kelly Radtke, Zoe Powis, Dawn Earl, Melissa Gabriel, Alvaro H. Serrano Russi, Lauren Brick, Mariya Kozenko, Emma Tham, Kimiyo M. Raymond, John A. Phillips, George E. Tiller, William G. Wilson, Rizwan Hamid, May C. V. Malicdan, Gen Nishimura, Giedre Grigelioniene, Andrew Jackson, Monte Westerfield, Michael B. Bober, William A. Gahl, Hudson H. Freeze
AMERICAN JOURNAL OF HUMAN GENETICS
(2018)
Article
Cell Biology
Xiaofeng Wang, Pingping Wang, Zhuangzhuang Zhang, Jean-Claude Farre, Xuezhi Li, Ruonan Wang, Zhijie Xia, Suresh Subramani, Changle Ma
Article
Genetics & Heredity
Carlos R. Ferreira, Wadih M. Zein, Laryssa A. Huryn, Andrea Merker, Seth I. Berger, William G. Wilson, George E. Tiller, Lynne A. Wolfe, Melissa Merideth, Daniel R. Carvalho, Angela L. Duker, Heiko Bratke, Marte Gjol Haug, Luis Rohena, Hanne B. Hove, Zhi-Jie Xia, Bobby G. Ng, Hudson H. Freeze, Melissa Gabriel, Alvaro H. Serrano Russi, Lauren Brick, Mariya Kozenko, Dawn L. Earl, Emma Tham, Gen Nishimura, John A. Phillips, William A. Gahl, Rizwan Hamid, Andrew P. Jackson, Giedre Grigelioniene, Michael B. Bober
GENETICS IN MEDICINE
(2020)
Article
Genetics & Heredity
Bobby G. Ng, Paulina Sosicka, Francois Fenaille, Annie Harroche, Sandrine Vuillaumier-Barrot, Mindy Porterfield, Zhi-Jie Xia, Shannon Wagner, Michael J. Bamshad, Marie-Christine Vergnes-Boiteux, Sophie Cholet, Stephen Dalton, Anne Dell, Thierry Dupre, Mathieu Fiore, Stuart M. Haslam, Yohann Huguenin, Tadahiro Kumagai, Michael Kulik, Katherine McGoogan, Caroline Michot, Deborah A. Nickerson, Tiffany Pascreau, Delphine Borgel, Kimiyo Raymond, Deepti Warad, Heather Flanagan-Steet, Richard Steet, Michael Tiemeyer, Nathalie Seta, Arnaud Bruneel, Hudson H. Freeze
Summary: The study identified a recurrent mutation in SLC37A4 causing a dominantly inherited congenital disorder of glycosylation characterized by coagulopathy and liver dysfunction with abnormal serum N-glycans. Liver-specific abnormalities in glycosylation were replicated in a CRISPR base-edited hepatoma cell line carrying the mutation. The mutant protein showed relocation to a non-Golgi compartment and altered Golgi morphology and reduced intraluminal pH, potentially explaining the glycosylation alterations.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Oncology
Kevin X. Liu, Helen H. Shang, Chantel Cacciotti, Emily Everdell, Ayal A. Aizer, Rifaquat Rahman, Seth Malinowski, David M. Meredith, Junne Kamihara, Patrick Y. Wen, Keith L. Ligon, Susan N. Chi, Karen J. Marcus, Kee Kiat Yeo, Sanda Alexandrescu, Daphne A. Haas-Kogan
Summary: The study aims to characterize clinical outcomes for adult and pediatric patients with primary CNS tumors harboring DICER1 mutations or loss of DICER1. The results showed that DICER1 mutations or loss are commonly found in diverse primary CNS tumors, with grade 3/4 glioma as the most common histology. Missense mutation in the DexD/H-box helicase domain was associated with prolonged survival in patients with grade 3/4 glioma.
JOURNAL OF NEURO-ONCOLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Bobby G. Ng, Zhi-Jie Xia, David Scott, Hudson H. Freeze