Subtle changes at the variable domain interface of the T-cell receptor can strongly increase affinity

An allosteric site in the T-cell receptor Cβ domain plays a critical signalling role

(2017) Kannan Natarajan et al.   Nature Communications

The Role of Protein Loops and Linkers in Conformational Dynamics and Allostery

(2016) Elena Papaleo et al.   CHEMICAL REVIEWS

Deep Mutational Scans as a Guide to Engineering High Affinity T Cell Receptor Interactions with Peptide-bound Major Histocompatibility Complex

(2016) Daniel T. Harris et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Differential scanning fluorimetry based assessments of the thermal and kinetic stability of peptide–MHC complexes

(2016) Lance M. Hellman et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Inhibition of RAS function through targeting an allosteric regulatory site

(2016) Russell Spencer-Smith et al.   Nature Chemical Biology

An Engineered Switch in T Cell Receptor Specificity Leads to an Unusual but Functional Binding Geometry

(2016) Daniel T. Harris et al.   STRUCTURE

Structural Model of the Extracellular Assembly of the TCR-CD3 Complex

(2016) Aswin Natarajan et al.   Cell Reports

New Strategies in Engineering T-cell Receptor Gene-Modified T cells to More Effectively Target Malignancies

(2015) T. M. Schmitt et al.   CLINICAL CANCER RESEARCH

Adaptability of protein structures to enable functional interactions and evolutionary implications

(2015) Turkan Haliloglu et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Identification of the Docking Site for CD3 on the T Cell Receptor β Chain by Solution NMR

(2015) Yanan He et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Pre–T-cell receptor binds MHC: Implications for thymocyte signaling and selection

(2015) Xinbo Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Distinct CDR3 Conformations in TCRs Determine the Level of Cross-Reactivity for Diverse Antigens, but Not the Docking Orientation

(2014) L. L. Jones et al.   JOURNAL OF IMMUNOLOGY

Measuring the activity of protein variants on a large scale using deep mutational scanning

(2014) Douglas M Fowler et al.   Nature Protocols

Changing the peptide specificity of a human T-cell receptor by directed evolution

(2014) Sheena N. Smith et al.   Nature Communications

A Unified View of “How Allostery Works”

(2014) Chung-Jung Tsai et al.   PLoS Computational Biology

Somatic Mutations of the Immunoglobulin Framework Are Generally Required for Broad and Potent HIV-1 Neutralization

(2013) Florian Klein et al.   CELL

Plasticity in the Contribution of T Cell Receptor Variable Region Residues to Binding of Peptide–HLA-A2 Complexes

(2013) Sheena N. Smith et al.   JOURNAL OF MOLECULAR BIOLOGY

Computational Design of a Protein-Based Enzyme Inhibitor

(2013) Erik Procko et al.   JOURNAL OF MOLECULAR BIOLOGY

Characterization of the Staphylococcal enterotoxin A: V  receptor interaction using human receptor fragments engineered for high affinity

(2013) P. Sharma et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Engineering antibodies by yeast display

(2012) Eric T. Boder et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

The spatial architecture of protein function and adaptation

(2012) Richard N. McLaughlin Jr et al.   NATURE

Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing

(2012) Timothy A Whitehead et al.   NATURE BIOTECHNOLOGY

Monoclonal TCR-redirected tumor cell killing

(2012) Nathaniel Liddy et al.   NATURE MEDICINE

Dissecting Protein−Protein Interactions Using Directed Evolution

(2011) Daniel A. Bonsor et al.   BIOCHEMISTRY

Enrich: software for analysis of protein function by enrichment and depletion of variants

(2011) Douglas M. Fowler et al.   BIOINFORMATICS

TCRs Used in Cancer Gene Therapy Cross-React with MART-1/Melan-A Tumor Antigens via Distinct Mechanisms

(2011) O. Y. Borbulevych et al.   JOURNAL OF IMMUNOLOGY

Interaction of Streptavidin-Based Peptide-MHC Oligomers (Tetramers) with Cell-Surface TCRs

(2011) J. D. Stone et al.   JOURNAL OF IMMUNOLOGY

Turning a protein kinase on or off from a single allosteric site via disulfide trapping

(2011) J. D. Sadowsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-resolution mapping of protein sequence-function relationships

(2010) Douglas M Fowler et al.   NATURE METHODS

Identification and engineering of human variable regions that allow expression of stable single-chain T cell receptors

(2010) D. H. Aggen et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The Fitness Landscapes of cis-Acting Binding Sites in Different Promoter and Environmental Contexts

(2010) Ryan K. Shultzaberger et al.   PLoS Genetics

The Importance of Being Tyrosine: Lessons in Molecular Recognition from Minimalist Synthetic Binding Proteins

(2009) Shohei Koide et al.   ACS Chemical Biology

Different Strategies Adopted by Kband Ldto Generate T Cell Specificity Directed against Their Respective Bound Peptides

(2009) Natalie A. Bowerman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Germ Line-governed Recognition of a Cancer Epitope by an Immunodominant Human T-cell Receptor

(2009) David K. Cole et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Different Thermodynamic Binding Mechanisms and Peptide Fine Specificities Associated with a Panel of Structurally Similar High-Affinity T Cell Receptors†‡

(2008) Lindsay L. Jones et al.   BIOCHEMISTRY

Understanding mechanisms governing protein–protein interactions from synthetic binding interfaces

(2008) A KOSSIAKOFF et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

T-cell receptor binding affinities and kinetics: impact on T-cell activity and specificity

(2008) Jennifer D. Stone et al.   IMMUNOLOGY

Backrub-Like Backbone Simulation Recapitulates Natural Protein Conformational Variability and Improves Mutant Side-Chain Prediction

(2008) Colin A. Smith et al.   JOURNAL OF MOLECULAR BIOLOGY

Structural features of T cell receptor variable regions that enhance domain stability and enable expression as single-chain VαVβ fragments

(2008) Sarah A. Richman et al.   MOLECULAR IMMUNOLOGY