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Biochemistry & Molecular Biology
Pragyesh Dixit, Swathi Shivaram Suratkal, Shrikant Babanrao Kokate, Debashish Chakraborty, Indrajit Poirah, Supriya Samal, Niranjan Rout, Shivaram P. Singh, Arup Sarkar, Asima Bhattacharyya
Summary: This study elucidates a novel mechanism of oxidative stress regulation by phosphorylated Siah2 in H. pylori-infected gastric epithelial cancer cells. Phosphorylation of Siah2 enhances the generation of reactive oxygen species (ROS) induced by H. pylori and leads to the degradation of the antioxidant protein GRP78. Surprisingly, cells expressing Siah2 with a phospho-null mutation have lower levels of cellular GRP78 but release more GRP78 and accumulate higher levels of ROS.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yulemi Gonzalez Quesada, Luc DesGroseillers
Summary: This study investigates the degradation mechanism of RNA-binding protein STAU1 in cancer cells. The research reveals that STAU1 degradation is related to the FPL-motif protein family and inflammation-related protein MAP4K1. Through experiments, it is confirmed that TRIM25 is the E3 ubiquitin ligase responsible for the degradation of STAU1 and MAP4K1. These findings are significant for further exploration of the inflammation mechanisms associated with cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hang Chen, Rui Yang, Lei Xing, Bin Wang, Dawei Liu, Xiaoqiang Ou, Yumei Deng, Rong Jiang, Junxia Chen
Summary: This study identified a novel hypoxia-induced circRNA, circPFKFB4, and demonstrated its role in promoting breast cancer progression. The study revealed an interaction between circPFKFB4 and the CRL4DDB2 E3 ubiquitin ligase and suggested circPFKFB4 as a potential biomarker and therapeutic target for breast cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Oncology
Tingmang Yan, Dapeng Zhou, Youwei Shi, Di Cui, Juntao Jiang, Bangmin Han, Shujie Xia, Zhou Wang, Haitao Liu, Wenhuan Guo, Yifeng Jing
Summary: The study revealed that AR stabilizes Siah2 protein by blocking its E3 ubiquitin ligase activity, while ADT reduces Siah2 protein expression but enhances its E3 ligase activity in PCa cells. The combination of ADT with Vit K3 shows promising results in delaying the occurrence of CRPC and inhibiting tumor growth.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Zeyun Hao, Jinfu Tian, Hong Fang, Liang Fang, Xiao Xu, Feng He, Shaoya Li, Wenya Xie, Qiang Du, Xiaoman You, Debao Wang, Qiuhong Chen, Ruyi Wang, Shimin Zuo, Meng Yuan, Guo-Liang Wang, Lanqin Xia, Yuese Ning
Summary: A ubiquitin E3 ligase-VQ protein-transcription factor module has been discovered to fine-tune plant immunity and growth by regulating gene expression at the transcriptional and posttranslational levels.
Article
Agronomy
Yongqing Yang, Qiao Su, Yurong Li, Zengshu Cheng, Yahui Song, Xinxin Jin, Jin Wang
Summary: This study identified stable major-effect QTL associated with peanut yield using transcriptome and genome sequencing, providing valuable information for cloning alleles with favorable effects on peanut yield.
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Chemistry, Medicinal
Adam G. Bond, Conner Craigon, Kwok-Ho Chan, Andrea Testa, Athanasios Karapetsas, Rotimi Fasimoye, Thomas Macartney, J. Julian Blow, Dario R. Alessi, Alessio Ciulli
Summary: This study describes the design and development of a new protein degradation system utilizing a variant of the Brd4 bromodomain as a degradation tag. The system effectively degrades BromoTagged proteins in a fast, selective manner, showing favorable pharmacokinetic profile in mice. This system expands the arsenal of chemical genetic degradation tools for manipulating protein levels and exploring therapeutic potential in cells and in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Martin P. Schwalm, Lena M. Berger, Maximilian N. Meuter, James D. Vasta, Cesear R. Corona, Sandra Roehm, Benedict-Tilman Berger, Frederic Farges, Sebastian M. Beinert, Franziska Preuss, Viktoria Morasch, Vladimir V. Rogov, Sebastian Mathea, Krishna Saxena, Matthew B. Robers, Susanne Mueller, Stefan Knapp
Summary: E3 ligases play a crucial role in regulating protein homeostasis by recruiting substrate proteins to the proteasomal degradation machinery. Recent research has focused on the Baculovirus IAP Repeat (BIR) family of E3 ligases, which contain a structurally conserved but diverse protein interaction domain. The Inhibitors of Apoptosis (IAP) family, which typically have three BIR domains, are promising drug targets. However, there is currently a lack of assay tools to evaluate the selectivity of inhibitors in this target area.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rui Zhang, Shaoqing Shi
Summary: HECT-type E3 ubiquitin ligases play a vital role in controlling protein function and stability, and members of the NEDD4 family have critical roles in dysregulation of autophagy in cancer cells. This review focuses on the role of NEDD4 E3 ligases in defective autophagy in cancer cells, discussing their function, substrates, and signaling pathways, providing a basis for cancer treatment through modulation of these ligases.
MOLECULAR MEDICINE
(2023)
Review
Immunology
Haoran Cui, Yaxian Zhang, Leiliang Zhang
Summary: Poxviruses have evolved various mechanisms to evade innate immunity, some of which involve poxvirus-encoded E3 ubiquitin ligases and adaptor proteins. These proteins can be categorized into five groups based on their functional domains and ubiquitin transfer mechanisms. Most known substrates of poxvirus E3 ubiquitin ligases are components of the innate immune system. Current research progress provides mechanistic insights into the interaction between these viruses and their hosts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Jialing Zhang, Chaonan Li, Long Li, Yajun Xi, Jingyi Wang, Xinguo Mao, Ruilian Jing
Summary: TaAIRP2-1B regulates spike length by facilitating TaHIPP3 degradation, and the haplotype Hap-1B-1 of TaAIRP2-1B is a favorable natural variation for increasing spike length in wheat. This study provides insights into the role of E3 ubiquitin ligase genes in wheat development and identifies TaAIRP2-1B as a key regulator of spike length. The findings also offer genetic resources and markers for wheat molecular breeding.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Ishita Tripathi-Giesgen, Christian Behrends, Arno F. Alpi
Summary: The ubiquitin system plays a crucial role in the host cellular defense program against bacterial infection, especially when certain bacteria are exposed to the host cytosol during invasion. Host cell E3 ubiquitin ligases contribute to the formation of a protective ubiquitin coat on invading pathogens, with their divergent ubiquitin conjugation mechanisms influencing the complexity of the anti-bacterial coating. Bacteria have evolved strategies to evade the activities of the host ubiquitin system.
Review
Biochemistry & Molecular Biology
Dong Wang, Yuanming Zou, Xinyue Huang, Zeyu Yin, Mohan Li, Jiaqi Xu, Boquan Wu, Da Li, Ying Zhang, Yingxian Sun, Xingang Zhang, Naijin Zhang
Summary: The ubiquitin-proteasome system is crucial for regulating protein levels in cells, and SMURF1 and SMURF2 are important components that maintain physiological processes by regulating the stability of multiple proteins. The regulatory functions of SMURFs in disease progression are complex, either facilitative or inhibitory, and understanding their mechanisms offers potential therapeutic targets and new avenues for research.
Review
Biochemistry & Molecular Biology
Dong Wang, Yuanming Zou, Xinyue Huang, Zeyu Yin, Mohan Li, Jiaqi Xu, Boquan Wu, Da Li, Ying Zhang, Yingxian Sun, Xingang Zhang, Naijin Zhang
Summary: The ubiquitin-proteasome system plays a crucial role in regulating protein levels in cells. SMURF1 and SMURF2 are key components in this system, responsible for regulating protein stability and maintaining physiological processes such as cell migration, proliferation, and apoptosis. They also play significant roles in disease progression, with complex regulatory functions. This review focuses on the mechanisms by which SMURF1 and SMURF2 regulate disease progression in non-cancerous diseases, providing potential therapeutic targets for various diseases and new research avenues for SMURF proteins.
Article
Multidisciplinary Sciences
Zhenwang Jie, Yu Zhang, Chen Wang, Bin Shen, Xin Guan, Zhihua Ren, Xinxin Ding, Wei Dai, Yongping Jiang
Article
Oncology
Byeong Hyeok Choi, Changyan Chen, Mark Philips, Wei Dai
Article
Cell & Tissue Engineering
Meng Qin, Xin Guan, Huihui Wang, Yu Zhang, Bin Shen, Qingyu Zhang, Wei Dai, Yupo Ma, Yongping Jiang
STEM CELL RESEARCH & THERAPY
(2017)
Article
Cell & Tissue Engineering
Yu Zhang, Chen Wang, Lan Wang, Bin Shen, Xin Guan, Jing Tian, Zhihua Ren, Xinxin Ding, Yupo Ma, Wei Dai, Yongping Jiang
STEM CELLS TRANSLATIONAL MEDICINE
(2017)
Article
Biochemistry & Molecular Biology
Byeong Hyeok Choi, Mark R. Philips, Yuan Chen, Lou Lu, Wei Dai
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Review
Pharmacology & Pharmacy
Xun Che, Wei Dai
CURRENT DRUG TARGETS
(2019)
Article
Biochemistry & Molecular Biology
Junlan Zhu, Grace Huang, Xiaohui Hua, Yang Li, Huiying Yan, Xun Che, Zhongxian Tian, Huating Liufu, Chao Huang, Jingxia Li, Jiheng Xu, Wei Dai, Haishan Huang, Chuanshu Huang
Article
Cell & Tissue Engineering
Yu Zhang, Bin Shen, Xin Guan, Meng Qin, Zhihua Ren, Yupo Ma, Wei Dai, Xinxin Ding, Yongping Jiang
STEM CELL RESEARCH & THERAPY
(2019)
Article
Medicine, Research & Experimental
Xin Guan, Lan Wang, Hanlu Wang, Huihui Wang, Wei Dai, Yongping Jiang
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Byeong Hyeok Choi, Ziyue Kou, Tania Marlyn Colon, Chih-Hong Chen, Yuan Chen, Wei Dai
Summary: Radil, a downstream effector of Ras, interacts strongly with KRas and GTP-bound Ras, suggesting a role in Ras activation. Overexpression of Radil leads to transient activation of MAPK signaling, while Radil knockdown weakens downstream Ras signaling, affecting cell proliferation, invasion, and migration. Radil plays a vital role in regulating Ras signaling, cell adhesion, and the epithelial-mesenchymal transition, potentially offering new opportunities for Ras-related anticancer drug development.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Oncology
Wei Dai, Suqing Xie, Changyan Chen, Byeong Hyeok Choi
Summary: Ras proteins play important roles in cell processes through posttranslational modifications, such as sumoylation, which can affect cell signaling, migration, and tumorigenesis. Inhibiting the sumoylation process can potentially suppress tumor development by blocking key cellular pathways.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Cell Biology
Honglei Jin, Lei Xue, Lan Mo, Dongyun Zhang, Xirui Guo, Jiheng Xu, Jingxia Li, Minggang Peng, Xuewei Zhao, Minghao Zhong, Dazhong Xu, Xue-Ru Wu, Haishan Huang, Chuanshu Huang
CELL ADHESION & MIGRATION
(2019)
Article
Cell Biology
Dazhong Xu, Wei Dai, Cen Li