期刊
JOURNAL OF BACTERIOLOGY
卷 199, 期 13, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00125-17
关键词
genome; identification; antibiotic resistance; metal resistance; antibacterial; biosynthesis; bulgecin
类别
资金
- Biotechnology and Biological Sciences Research Council [BB/L01758X/1, BB/L021692/1]
- Medical Research Council [MR/L015080/1]
- Life Science Research Network Wales [NRNSep14036]
- Biochemical Society
- University of Bristol
- Cardiff University
- Swansea University
- BBSRC [BB/L01758X/1, BB/L021692/1] Funding Source: UKRI
- EPSRC [EP/K03927X/1] Funding Source: UKRI
- MRC [MR/L015080/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L021692/1, BB/L01758X/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/K03927X/1] Funding Source: researchfish
- Medical Research Council [MR/L015080/1] Funding Source: researchfish
Pseudomonas mesoacidophila ATCC 31433 is a Gram-negative bacterium, first isolated from Japanese soil samples, that produces the monobactam isosulfazecin and the beta-lactam-potentiating bulgecins. To characterize the biosynthetic potential of P. mesoacidophila ATCC 31433, its complete genome was determined using single-molecule real-time DNA sequence analysis. The 7.8-Mb genome comprised four replicons, three chromosomal (each encoding rRNA) and one plasmid. Phylogenetic analysis demonstrated that P. mesoacidophila ATCC 31433 was misclassified at the time of its deposition and is a member of the Burkholderia cepacia complex, most closely related to Burkholderia ubonensis. The sequenced genome shows considerable additional biosynthetic potential; known gene clusters for malleilactone, ornibactin, isosulfazecin, alkylhydroxyquinoline, and pyrrolnitrin biosynthesis and several uncharacterized biosynthetic gene clusters for polyketides, nonribosomal peptides, and other metabolites were identified. Furthermore, P. mesoacidophila ATCC 31433 harbors many genes associated with environmental resilience and antibiotic resistance and was resistant to a range of antibiotics and metal ions. In summary, this bioactive strain should be designated B. cepacia complex strain ATCC 31433, pending further detailed taxonomic characterization. IMPORTANCE This work reports the complete genome sequence of Pseudomonas mesoacidophila ATCC 31433, a known producer of bioactive compounds. Large numbers of both known and novel biosynthetic gene clusters were identified, indicating that P. mesoacidophila ATCC 31433 is an untapped resource for discovery of novel bioactive compounds. Phylogenetic analysis demonstrated that P. mesoacidophila ATCC 31433 is in fact a member of the Burkholderia cepacia complex, most closely related to the species Burkholderia ubonensis. Further investigation of the classification and biosynthetic potential of P. mesoacidophila ATCC 31433 is warranted.
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