期刊
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
卷 20, 期 2, 页码 381-385出版社
KOREAN SOC APPLIED ENTOMOLOGY
DOI: 10.1016/j.aspen.2017.01.012
关键词
Mdlettia pachycarpa; Antifeedant activity; No-choice test method; Spodoptera litura; Biopeslicide
类别
资金
- Indian Council of Agricultural Research, New Delhi
This study was aimed to evaluate the antifeedant activity of leaf extracts on third instar larvae of Tobacco Leaf Eating Caterpillar (Spodoptera litura) and bioassay guided isolation of pure compounds from Millettia pachycarpa. Plant material was extracted with two different solvents; hexane and methanol. Methanol extract was found to be active as compared to hexane extract against S. litura after 48 h and was sequentially further partitioned between hexane, dichloromethane and butanol. DCM fraction was further subjected to column chromatography from which five fractions were obtained. Among different CC fractions, fraction 2 (FR 2) showed highest activity (Al-50 227.12 ppm) and pure compound, lupeol, was isolated. Antifeedant activity results show that activity was comparable to natural plant based insecticide azadirachtin and this plant can be further explored for its use as natural pesticide. (C) 2017 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
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