Article
Medicine, General & Internal
Patrik Mik, Katsiaryna Barannikava, Polina Surkova
Summary: Liver fibrosis assessment usually involves staining liver tissue with Masson's trichrome or picrosirius red to quantify liver connective tissue (LCT) and determine fibrosis score. However, there have been concerns about the accuracy of these semiquantitative assessments, and significant variations in the amount of LCT in healthy rats have been observed. To address this reproducibility crisis in science, it is crucial to examine the results and methods, and develop an unbiased and reproducible method for LCT assessment.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biology
Esteban Andres Sanchez-Jaramillo, Luz Elena Gasca-Lozano, Jose Maria Vera-Cruz, Luis Daniel Hernandez-Ortega, Adriana Maria Salazar-Montes
Summary: The article introduces a method of using computer-assisted fast automated analysis to evaluate the degree of kidney fibrosis in animal models. This method is faster and easier than traditional image-by-image analysis, helping researchers to conduct studies more efficiently and discover new treatments.
Article
Medicine, General & Internal
Lauren C. Testa, Yvon Jule, Linnea Lundh, Karine Bertotti, Melissa A. Merideth, Kevin J. O'Brien, Steven D. Nathan, Drew C. Venuto, Souheil El-Chemaly, May Christine V. Malicdan, Bernadette R. Gochuico
Summary: Automated computerized imaging analysis has been shown to provide accurate and reader-independent quantification of pulmonary fibrosis in human histopathology samples. Fibrosis, collagen content, and immune-stained cells can be automatically and individually quantified from serial sections, enhancing the tools available for studying fibrotic lung disease. This analysis method allows for continuous measurement of fibrosis in lung tissue, with potential applications for both research and clinical diagnosis.
FRONTIERS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Cindy Serdjebi, Karine Bertotti, Pinzhu Huang, Guangyan Wei, Disha Skelton-Badlani, Isabelle A. Leclercq, Damien Barbes, Bastien Lepoivre, Yury Popov, Yvon Jule
Summary: This study utilized an automated image analysis software to quantitatively assess fibrosis deposition in two preclinical models of liver fibrosis and established correlations with other quantitative fibrosis descriptors. The results demonstrate that automated digital analysis is a reliable tool for evaluating the dynamics of liver fibrosis and efficacy of antifibrotic drug candidates in preclinical models.
SCIENTIFIC REPORTS
(2022)
Article
Crystallography
Magnus. J. J. Anderson, Hector. C. C. Basoalto
Summary: This article presents automated stereological methods for approximating the 3D size distribution of unimodal or bimodal precipitate dispersions using 2D and 1D measurements from polydisperse spherical non-penetrating particle dispersions. A method to quantify the uncertainty of the approximation based on the number of sampled particles is introduced and validated with experimental data. Derivation and verification of the analytical stereological expressions are included. Two procedures are presented for estimating the 3D size distribution of bimodal particle populations, depending on the relative size of the populations. These methods are valuable for determining the initial particle size distribution for modeling and characterizing precipitate dispersions.
Article
Oncology
Nicolas Elie, Florence Giffard, Cecile Blanc-Fournier, Pierre-Marie Morice, Pierre-Emmanuel Brachet, Soizic Dutoit, Benoit Plancoulaine, Laurent Poulain
Summary: This study developed a fully automated and robust method for quantifying protein biomarker expression in high-grade serous ovarian tumors. By considering staining heterogeneity and using mathematical thresholds, the method can be used to guide pathologists in therapeutic decision-making. The method showed good correlation with pathologists' classification and is replicable for analyzing other biological and medical issues.
FRONTIERS IN ONCOLOGY
(2022)
Article
Chemistry, Physical
Ilona Jastrzebska, Adam Piwowarczyk
Summary: Image analysis is a powerful tool with applications in scientific research, industry, and everyday life. In materials science, there is still potential for further use of image analysis. Interdisciplinary cooperation between materials scientists and computer scientists can unlock the potential of digital image analysis. Traditional and cutting-edge tools for image analysis in materials science allow for quantitative assessment of coexisting components, but the automated method using a developed algorithm shows promising results in terms of speed and accuracy for microstructure analysis.
Article
Microbiology
Yajie Chen, Henghui He, Licheng Luo, Kangyi Liu, Min Jiang, Shiqi Li, Xianqi Zhang, Xin Yang, Qian Liu
Summary: A novel method utilizing artificial intelligence techniques for analyzing fibro-pathological images allows for standardized, precise, and staged diagnosis of pulmonary fibrosis. Analysis of COVID-19 lung tissue microscopic images revealed a significant correlation between the number of macrophage aggregates and the degree of fibrosis, particularly the M2-type macrophages. This method advancement can contribute to the understanding and prevention of pulmonary fibrosis, and further exploration of the specific molecular mechanisms of M2-type macrophage polarization.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Sampsa Kohtala, Tonje Marie Vikene Nedal, Carlo Kriesi, Siv Helen Moen, Qianli Ma, Kristin Sirnes Odegaard, Therese Standal, Martin Steinert
Summary: This study presents an efficient and reliable method to quantify human osteoclasts using machine learning, avoiding the reliance on trained personnel in traditional methods.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Matteo Giardino, Valentina Balestra, Davide Janner, Rossana Bellopede
Summary: Microplastics are small solid polymers with dimensions less than 5 mm that contaminate the environment. Visual count is commonly used for their detection and quantification, but it is time-consuming and prone to errors. To address this issue, a semi-automatic image processing method was developed for quantifying and measuring microplastics on filter membrane substrates, providing accurate and fast results.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Cardiac & Cardiovascular Systems
Eliot G. Peyster, Sara Arabyarmohammadi, Andrew Janowczyk, Sepideh Azarianpour-Esfahani, Miroslav Sekulic, Clarissa Cassol, Luke Blower, Anil Parwani, Priti Lal, Michael D. Feldman, Kenneth B. Margulies, Anant Madabhushi
Summary: The study aimed to demonstrate that cellular rejection grades generated via computational histological analysis are comparable to those provided by expert pathologists, showing that the computational method can provide expert-quality rejection grading within the range of inter-grader variability seen among human pathologists.
EUROPEAN HEART JOURNAL
(2021)
Article
Cardiac & Cardiovascular Systems
Eliot G. Peyster, Sara Arabyarmohammadi, Andrew Janowczyk, Sepideh Azarianpour-Esfahani, Miroslav Sekulic, Clarissa Cassol, Luke Blower, Anil Parwani, Priti Lal, Michael D. Feldman, Kenneth B. Margulies, Anant Madabhushi
Summary: The study demonstrates that a computational histological analysis tool, CACHE-Grader, can provide expert-quality rejection grading that is on par with human pathologists, showing nearly identical performance in both internal and external validation sets. The tool has the ability to accurately reproduce the clinical standard for cellular rejection diagnosis and has superior sensitivity for high-grade rejection.
EUROPEAN HEART JOURNAL
(2021)
Article
Rheumatology
Ju Hyun Oh, Grace Hyun J. Kim, Gary Cross, Joseph Barnett, Joseph Jacob, Seokchan Hong, Jin Woo Song
Summary: An automated quantification system (AQS) can be used to predict the prognosis of rheumatoid arthritis-associated interstitial lung disease (RA-ILD), and high QLF scores can differentiate a poor prognostic phenotype similar to idiopathic pulmonary fibrosis (IPF).
Article
Cell Biology
Pawel Rubic, Ewa Dziewiecka, Magdalena Szymanska, Robert Banys, Malgorzata Urbanczyk-Zawadzka, Maciej Krupinski, Malgorzata Mielnik, Sylwia Wisniowska-Smialek, Aleksandra Karabinowska, Piotr Podolec, Mateusz Winiarczyk, Matylda Gliniak, Monika Kaciczak, Jan Robak, Arman Karapetyan, Ewa Wypasek
Summary: The relationship between circulating fibrosis markers and cardiac fibrosis in dilated cardiomyopathy (DCM) remains unclear. While circulating fibrosis markers were not associated with replacement or interstitial fibrosis, cardiac-specific markers such as NT-proBNP and hs-TnT showed associations with fibrosis in DCM patients. Levels of circulating fibrosis markers may not be useful for diagnosing or monitoring cardiac fibrosis in DCM.
Article
Computer Science, Information Systems
Anicetus Odo, Ramon Muns, Laura Boyle, Ilias Kyriazakis
Summary: Ear biting is a welfare challenge in commercial pig farming, and early detection and management are important for animal health and productivity. This study presents an automated detection pipeline using deep learning to identify ear biting outbreaks. The pipeline successfully detects and tracks ear biting instances in videos, achieving high precision values. The method can be used as the basis for an early warning system in commercial farms.
Article
Cardiac & Cardiovascular Systems
Dawud Abawi, Tommaso Rinaldi, Alessandro Faragli, Burkert Pieske, Daniel A. Morris, Sebastian Kelle, Carsten Tschoepe, Concetta Zito, Alessio Alogna
Summary: PV analysis is a comprehensive way to describe cardiac function, but is still highly invasive and time-consuming. Traditional echocardiographic parameters fall short in describing LV function. The introduction of LV pressure-strain analysis as a new technique provides new insights in assessing myocardial work in a non-invasive fashion.
HEART FAILURE REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Ewald Kolesnik, Daniel Scherr, Ursula Rohrer, Martin Benedikt, Martin Manninger, Harald Sourij, Dirk von Lewinski
Summary: SGLT2 inhibitors have shown promising effects in reducing hospitalization rates for heart failure, and there have been reports of their potential antiarrhythmic properties. However, the mechanisms behind their effects on arrhythmias are still unclear. Prospective trials are currently underway to further investigate their impact on arrhythmias.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Analytical
Ursula Rohrer, Martin Manninger, Andreas Zirlik, Daniel Scherr
Summary: A wearable cardioverter-defibrillator (WCD) is a temporary treatment option for high-risk patients and those who are not suitable for an implantable cardioverter defibrillator (ICD). It is also increasingly important for the detection and treatment of heart failure (HF) and its arrhythmias. The WCD has evolved from a shock device to a monitoring device that captures physical activity and biomarkers for HF. In addition to preventing sudden cardiac death, the WCD could become an important tool in managing heart failure through remote monitoring and tracking therapy success.
Article
Cardiac & Cardiovascular Systems
Astrid N. L. Hermans, Monika Gawalko, Henrike A. K. Hillmann, Afzal Sohaib, Rachel M. J. van der Velden, Konstanze Betz, Dominique Verhaert, Daniel Scherr, Julia Meier, Arian Sultan, Daniel Steven, Elena Terentieva, Ron Pisters, Martin Hemels, Leonard Voorhout, Piotr Lodzinski, Bartosz Krzowski, Dhiraj Gupta, Nikola Kozhuharov, Henri Gruwez, Kevin Vernooy, Nikki A. H. A. Pluymaekers, Jeroen M. Hendriks, Martin Manninger, David Duncker, Dominik Linz
Summary: TeleCheck-AF is a mobile health infrastructure that incorporates mobile app-based heart rate and rhythm monitoring for atrial fibrillation patients. The study found that self-reported assessment of risk factors had high accuracy, but limited accuracy for some components of the CHA(2)DS(2)-VASc score. Direct assessment by healthcare professionals is still necessary for high quality clinical decision-making.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Monika Gawalko, Astrid Nl Hermans, Rachel Mj van Der Velden, Konstanze Betz, Dominique Vm Verhaert, Henrike Ak Hillmann, Daniel Scherr, Julia Meier, Arian Sultan, Daniel Steven, Elena Terentieva, Ron Pisters, Martin Hemels, Leonard Voorhout, Piotr Lodzinski, Bartosz Krzowski, Dhiraj Gupta, Nikola Kozhuharov, Laurent Pison, Henri Gruwez, Lien Desteghe, Hein Heidbuchel, Stijn Evens, Emma Svennberg, Tom de Potter, Kevin Vernooy, Nikki Aha Pluymaekers, Martin Manninger, David Duncker, Afzal Sohaib, Dominik Linz, Jeroen M. Hendriks
Summary: In the TeleCheck-AF project, more than one-fourth of patients with atrial fibrillation had optimal motivation and adherence to app-based heart rate/rhythm monitoring. Older age and absence of diabetes were predictors of optimal motivation/adherence.
EUROPEAN JOURNAL OF CARDIOVASCULAR NURSING
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Jonas L. Isaksen, Martin Manninger, Dominik Linz
JOURNAL OF INTERVENTIONAL CARDIAC ELECTROPHYSIOLOGY
(2023)
Article
Physiology
Karli Gillette, Matthias A. F. Gsell, Marina Strocchi, Thomas Grandits, Aurel Neic, Martin Manninger, Daniel Scherr, Caroline H. Roney, Anton J. Prassl, Christoph M. Augustin, Edward J. Vigmond, Gernot Plank
Summary: Virtual heart technologies have the potential to represent the individual electrophysiology of the heart in computer models. However, their widespread clinical use is limited by computational costs and difficulty in validation. In this study, a novel virtual technology was developed to overcome these limitations and accurately represent the electrophysiology of all four-chambers of the heart. The personalized virtual heart technology was able to replicate standard clinical morphological ECG features under different conditions, demonstrating its clinical viability.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Medicine, General & Internal
Birgit Zirngast, Leonhard Berboth, Martin Manninger, Helmut Hinghofer-Szalkay, Daniel Scherr, Lonnie G. Petersen, Nandu Goswami, Alessio Alogna, Heinrich Maechler
Summary: This study aimed to investigate the immediate hemodynamic alterations occurring during lower body negative pressure (LBNP) in healthy Landrace pigs. The findings demonstrated that incremental LBNP consistently induced a preload dependent depression of systemic hemodynamics according to the Frank-Starling mechanism, and the abrupt release of LBNP could revert these changes to baseline values within a short period of time.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pediatrics
Stefan Kurath-Koller, Martin Manninger, Nathalie Oeffl, Martin Koestenberger, Hannes Sallmon, Joachim Will, Daniel Scherr
Summary: The study showed that algorithms predicting AP location in pediatric patients with Wolff-Parkinson-White syndrome have low accuracy for exact localization, but higher accuracy for distinguishing the laterality of the AP.
Article
Cardiac & Cardiovascular Systems
Astrid N. L. Hermans, Jonas L. Isaksen, Monika Gawalko, Nikki A. H. A. Pluymaekers, Rachel M. J. van der Velden, Hilco Snippe, Stijn Evens, Glenn De Witte, Justin G. L. M. Luermans, Martin Manninger, Joost Lumens, Jorgen K. Kanters, Dominik Linz
Summary: This study aimed to evaluate the accuracy of continuous PPG-based 1 min mean heart rate assessment during persistent AF. By comparing PPG-based smartwatch and Holter ECG monitoring in 50 AF patients, it was found that PPG technology showed high accuracy and small estimation error in 1 min mean heart rate compared with Holter ECG. Only chronic heart failure was associated with a lower agreement between ECG-derived and PPG-derived heart rates.
Article
Cardiac & Cardiovascular Systems
Julie Norup Hertel, Kezia Jerltorp, Malthe Emil Hotbjerg Hansen, Jonas L. L. Isaksen, Stefan Michael Sattler, Benedikt Linz, Sevasti-Maria Chaldoupi, Thomas Jespersen, Arnela Saljic, Uffe Gang, Martin Manninger, Dominik Linz
Summary: This study proposes a standardized workflow for 3D-electroanatomical mapping guided pulmonary vein isolation in pigs. The study conducted procedures such as femoral vein puncture, blood pressure measurement, intracardiac ultrasound-guided puncture, and 3D-electroanatomical mapping of the left atrium. The results show that reproducible and safe pulmonary vein isolation can be achieved in pigs using current technologies and a step-by-step approach.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Critical Care Medicine
Simon Orlob, Stephan Grundner, Johannes Wittig, Michael Eichinger, Felix Pucher, Michael Eichlseder, Raphaela Lingitz, Martin Rief, Niklas Palt, Charlotte Hartwig, Gregor Zangl, Markus Haar, Martin Manninger, Ursula Rohrer, Daniel Scherr, Andreas Zirlik, Gerhard Prause, David Zweiker
Summary: This study analysed the knowledge and willingness to perform basic life support (BLS) of laypersons passing an automated external defibrillator (AED) at a public train station. The study found that there was an increase in knowledge of BLS actions in the lay public after a structured regional awareness campaign. However, severe gaps in BLS knowledge still exist.
RESUSCITATION PLUS
(2023)
Article
Medicine, General & Internal
Ursula Rohrer, Martin Manninger, Lukas Fiedler, Clemens Steinwender, Ronald K. Binder, Markus Stuehlinger, Birgit Zirngast, David Zweiker, Andreas Zirlik, Daniel Scherr, Austrian WCD Study Grp
Summary: This study investigated the outcomes of a group of acute myocardial infarction (AMI) patients in Austria who wore a wearable cardioverter defibrillator (WCD). The results showed that despite good compliance with wearing the WCD, the rate of arrhythmic mortality was not significantly different compared to other studies.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Dirk von Lewinski, Lukas Herold, Eva Bachl, Heiko Bugger, Theresa Glantschnig, Ewald Kolesnik, Nicolas Verheyen, Martin Benedikt, Markus Wallner, Friederike von Lewinski, Albrecht Schmidt, Stefan Harb, Klemens Ablasser, Michael Sacherer, Daniel Scherr, Martin Manninger-Wuenscher, Sascha Paetzold, Johannes Gollmer, Andreas Zirlik, Gabor G. Toth
Summary: This study compared the eligibility criteria of the ECLS-SHOCK trial with a real-world cohort of patients with cardiogenic shock. The results showed that only 38% of patients in the real-world cohort met the eligibility criteria of the ECLS-SHOCK trial, and the differences in baseline characteristics may affect the use of VA-ECMO and patient outcome parameters.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Louisa O'Neill, Milad El Haddad, Benjamin Berte, Richard Kobza, Gabriela Hilfiker, Daniel Scherr, Martin Manninger, Adrianus P. Wijnmaalen, Serge A. Trines, Jean-Yves Wielandts, Kris Gillis, Michelle Lycke, Benjamin De Becker, Rene Tavernier, Jean-Benoit Le Polain De Waroux, Sebastien Knecht, Mattias Duytschaever
Summary: High-power, short-duration ablation for pulmonary vein isolation can reduce procedural times, but may affect lesion quality. This study compared the efficiency, efficacy, and safety of using 90-W/4-second ablation to 35/50-W ablation in atrial fibrillation patients undergoing PVI.
JACC-CLINICAL ELECTROPHYSIOLOGY
(2022)