Article
Plant Sciences
B. M. Ravikumara, G. Ramanagouda, M. K. Naik, Rameshwar Telangre, Mamta Sharma
Summary: This study investigated the pathogenic diversity and distribution of F. udum variants in major pigeonpea growing regions of India. Variant 2 and 3 were found to be widespread and predominant, while variant 7 and 8 exhibited high virulence. Three pigeonpea cultivars, ICP 9174, BDN-2, and Bahar (ICP 7197), showed resistance to most F. udum isolates.
Article
Multidisciplinary Sciences
Arnab Purohit, Sanatan Ghosh, Shreeparna Ganguly, Madan Singh Negi, Shashi Bhushan Tripathi, Rituparna Kundu Chaudhuri, Dipankar Chakraborti
Summary: Transcriptomic profiling of pigeonpea plants inoculated with Fusarium udum identified several defense responsive pathways, including jasmonic acid and salicylic acid mediated defense responses, cell wall remodeling, vascular development and pattering, abscisic acid mediated responses, effector triggered immunity, and reactive oxygen species mediated signaling. These pathways play crucial roles in defense responses of Fusarium udum inoculated resistant plants.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Raj K. Mishra, Sonika Pandey, K. K. Hazra, Monika Mishra, S. J. Satheesh Naik, Abhishek Bohra, Ashok Kumar Parihar, U. S. Rathore, Krishna Naimuddin, Krishna Kumar, Bansa Singh, N. P. Singh
Summary: This study identified efficient Trichoderma isolates for controlling Fusarium wilt in pigeonpea by inducing defense-related enzymes. Among the tested isolates, IIPRTh-31 and IIPRTh-33 showed significant antipathogenic activity and disease suppression.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Chemistry, Applied
Amit Kumar Maurya, Vinny Aditya, Vinny John, Hemlata Pant, Sujata P. Sharma, Doaa Z. El-Refaey, Rokayya Sami, Mahmoud Helal, Fadi Baakdah, Nevin Ahmed
Summary: Pigeonpea is a versatile crop with significant economic importance. Fusarium wilt caused by Fusarium udum is a major economic threat to pigeonpea growers, but the study found that the use of neem cake effectively suppressed the disease and improved the growth and root-nodule count of pigeonpea.
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
(2023)
Article
Microbiology
Alok K. Srivastava, Ruchi Srivastava, Jagriti Yadav, Alok K. Singh, Praveen K. Tiwari, Anchal K. Srivastava, Pramod K. Sahu, Shiv M. Singh, Prem Lal Kashyap
Summary: This study focused on the analysis of the whole genome of Fusarium udum, a pathogen causing wilt in pigeon pea. Through de novo assembly, a total of 16,179 protein-coding genes were identified, with 11,892 (73.50%) genes annotated using BlastP and 8,928 (55.18%) genes from KOG annotation. In addition, 5,134 unique InterPro domains were detected in the annotated genes. The analysis also identified 1,060 genes (6.55%) as virulence genes and 1,439 secretory proteins in the secretome profiling. Comparative mining revealed common and specific effectors related to host cell death in F. udum. The study highlights the importance of deciphering the whole genome of F. udum in understanding various aspects of the pathogen.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Health Care Sciences & Services
Yusuke Saigusa, Shinto Eguchi, Osamu Komori
Summary: The generalized linear mixed model (GLMM) is a common method for analyzing longitudinal and clustered data in biological sciences. However, issues of model complexity and misspecification can arise. This paper extends the standard GLMM to a nonlinear mixed-effects model based on quasi-linear modeling, providing an estimation algorithm and a conditional AIC for the proposed model. Performance under model misspecification is evaluated in simulation studies, and the proposed model is shown to capture heterogeneity in respiratory illness data.
STATISTICAL METHODS IN MEDICAL RESEARCH
(2022)
Article
Agronomy
Rachit K. Saxena, Anil Hake, Abhishek Bohra, Aamir W. Khan, Anupama Hingane, Rafat Sultana, Indra Prakash Singh, S. J. Satheesh Naik, Rajeev K. Varshney
Summary: In this study, a novel approach was used to develop a diagnostic kit for identification of suitable FW and SMD resistant lines, with 10 markers each for FW and SMD resistance. Investigation of the diversity of these loci has shown the role of different alleles in different resistant genotypes. Two genes for FW resistance and four genes for SMD resistance have been identified, along with a customized and cost-effective genotyping assay to encourage their downstream applications in pigeonpea breeding programs.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Multidisciplinary Sciences
Geofroy Kinhoegbe, Gustave Djedatin, Rachit Kumar Saxena, Anu Chitikineni, Prasad Bajaj, Johiruddin Molla, Clement Agbangla, Alexandre Dansi, Rajeev Kumar Varshney
Summary: The genetic diversity and population structure of pigeonpea in Benin were revealed using the GBS approach. This study found four clear subpopulations and a high gene exchange between them. The results suggest that the genetic diversity and population structure can be utilized for improving genetic gain in pigeonpea breeding programs in Benin through Genome Wide Association Studies and Marker-Assisted Selection.
Article
Agronomy
Charu Bisht, S. K. Verma, A. K. Gaur, C. Chauhan, Harsh Deep, Anandi Karn, R. K. Sharma
Summary: This study characterized pigeonpea genotypes for Fusarium wilt resistance at both morphological and molecular level. The results revealed specific markers that were highly effective in differentiating resistant and susceptible genotypes, and identified a superior genotype with high yield and resistance against wilt.
Article
Mathematics, Applied
Nesrin Guler, Melek Eris Buyukkaya
Summary: This article compares a predictor with the best linear unbiased predictor (BLUP) for a unified form of all unknown parameters under a stochastically restricted linear mixed model (SRLMM) in terms of the mean squared error matrix (MSEM) criterion. The methodology of block matrix inertias and ranks is employed to compare the MSEMs of these predictors. The comparison results are also demonstrated for a linear mixed model with and without an exact restriction, as well as special cases of the unified form of all unknown parameters in the SRLMM.
Article
Agriculture, Multidisciplinary
Triveni Sodimalla, Nagaraju Yalavarthi
Summary: Emerging plant diseases have become a significant threat to the global economy in recent years. The resistance of pathogens to fungicides and climate change are the main causes of outbreaks. Silver nanoparticles have shown the ability to effectively restrain pathogenic bacteria, fungi, and viruses, while minimizing the risk of resistance and phytotoxicity. However, more research is needed to assess their toxicity and address concerns about environmental safety and production costs before they can be widely used in agriculture.
ANNALS OF APPLIED BIOLOGY
(2022)
Article
Mathematical & Computational Biology
Hongqian Wu, Michael P. Jones
Summary: The study proposes an extension of the generalized linear model for analyzing longitudinal data by incorporating random effects and using maximum likelihood for estimation and inference. Simulation studies are conducted to assess its properties and compare it to the generalized linear mixed model, with an illustration using data from a longitudinal study of Huntington's disease.
STATISTICS IN MEDICINE
(2021)
Article
Plant Sciences
Raina Bajpai, Gagan Kumar, Birinchi Kumar Sarma
Summary: The study identified key CcERF genes important for resistance to F. udum WSP-V2 and showed that under combined stress, the expression of most genes was regulated, suggesting cross-talk of signaling pathways.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Longfei Wu, Rudolph Fredua-Agyeman, Stephen E. Strelkov, Kan-Fa Chang, Sheau-Fang Hwang
Summary: This study aimed to detect quantitative trait loci (QTL) conferring partial resistance to Fusarium root rot and wilting in field pea. The study identified stable QTL and significant digenic epistatic interactions, which are important for breeding disease-resistant pea varieties and developing markers.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Wanderson Bucker Moraes, Laurence V. V. Madden, Byung-Kee Baik, James Gillespie, Pierce A. A. Paul
Summary: Fusarium head blight (FHB) of wheat, caused by Fusarium graminearum, leads to grain contamination with the mycotoxin deoxynivalenol (DON). This study aimed to quantify the effects of temperature, relative humidity (RH), and preharvest rainfall on DON, DON-3-glucoside (D3G), and their relationship. Results showed that higher RH and lower temperature favored D3G formation. Rainfall increased D3G levels and induced preharvest sprouting. These findings provide valuable insights into the complex disease-mycotoxin system.
Review
Agronomy
Paras Pandey, Meenal Rathore, N. P. Singh
Summary: Black gram is an important pulse cultivated throughout India, but its production and productivity are constrained by various stresses. While technology has aided in genetic improvement, the narrow genetic base remains a potential issue. In vitro regeneration and genetic transformation have helped in improving the genetics of black gram, but further research and utilization are needed.
Article
Biochemistry & Molecular Biology
Alok Das, Alok Shukla, Shallu Thakur, Meenal Rathore, Narendra Pratap Singh
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Alok Das, Partha Sarathi Basu, Manoj Kumar, Jamal Ansari, Alok Shukla, Shallu Thakur, Parul Singh, Subhojit Datta, Sushil Kumar Chaturvedi, M. S. Sheshshayee, Kailash Chandra Bansal, Narendra Pratap Singh
Summary: Transgenic chickpea lines carrying stress-inducible AtDREB1a gene exhibited improved drought tolerance with higher relative water content, longer chlorophyll retention capacity, and enhanced osmotic adjustment under severe drought stress. These transgenic lines also showed higher photosynthetic efficiency and water use efficiency, resulting in higher seed yield compared to control under progressively drier conditions. The study concludes that the selected transgenic chickpea event, based on precise phenotyping, is a valuable resource for breeding programs aimed at enhancing drought tolerance in chickpea.
Article
Genetics & Heredity
Abhishek Bohra, Abhishek Rathore, Prasad Gandham, Rachit K. Saxena, S. J. Satheesh Naik, Dibendu Dutta, Indra P. Singh, Farindra Singh, Meenal Rathore, Rajeev K. Varshney, Narendra P. Singh
Summary: Cytoplasmic male sterility (CMS) is a unique system for understanding cytoplasmic nuclear crosstalk and exploiting hybrid vigor in crops. Transcriptome profiling of pigeonpea A4-CMS line revealed significant gene expression differences in later stages of floral bud development. This study provides insights into the functional genomics of CMS trait and candidate genes for pigeonpea pollen development.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2021)
Article
Entomology
Revanasidda Aidbhavi, Aditya Pratap, Prasoon Verma, Amrit Lamichaney, Sanjay M. Bandi, S. D. Nitesh, Mohd Akram, Meenal Rathore, Bansa Singh, Narendra P. Singh
Summary: This study screened 42 endemic wild Vigna accessions from 13 species against three cosmopolitan bruchid species and identified highly resistant and resistant lines that could be further analyzed for development of bruchid resistant superior cultivars.
JOURNAL OF STORED PRODUCTS RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Vinaya Tiwari, Alok Das, Shallu Thakur, Rakesh Kumar Trivedi
Summary: India heavily relies on imported fossil fuels, causing a significant economic burden and environmental impact. Transitioning to carbon-neutral renewable fuels like algal biodiesel is essential for sustainability. Research on different algal strains for biodiesel production and fuel properties comparison is crucial for selecting the most suitable species.
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2021)
Article
Plant Sciences
Mohd Akram, Naimuddin Kamaal, Aditya Pratap, Abdul Muin, Aniruddha Kumar Agnihotri, Utkarsh Singh Rathore, Meenal Rathore, Narendra Pratap Singh
Summary: PCR assays of moth bean samples affected by yellow mosaic disease (YMD) identified the presence of three viruses (MYMIV, MYMV, and HgYMV) in two samples and two of these viruses in the remaining six samples. Sequence analysis confirmed the involvement of multiple DNA-A and DNA-B molecules in the samples, and recombination events were detected. Phylogenetic analysis showed the grouping of the studied viruses with related begomoviruses. This study reports the first association of HgYMV with moth bean yellow mosaic disease and indicates the potential for the development of new virus strains/species through viral mixed infections.
JOURNAL OF PHYTOPATHOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Prateek Singh, Alok Shukla, Neeraj Nath Tiwari, Jamal Ansari, Shallu Thakur, Prakash G. Patil, Meenal Rathore, O. P. Verma, Narendra Pratap Singh, Alok Das
Summary: This study presents a protocol for the direct transformation of chickpea with high efficiency, allowing for rapid, routine, and efficient modification of its traits and capabilities.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Multidisciplinary Sciences
Prateek Singh, G. K. Sujayanand, Shallu Thakur, Meenal Rathore, Om Prakash Verma, Narendra Pratap Singh, Alok Das
Summary: In this study, genetically engineered chickpea lines carrying codon modified Vip3Aa gene were evaluated for their efficacy against gram pod borer, a Lepidopteran insect pest. The results showed that the transgenic lines exhibited significant insecticidal effects, reducing larval survival, feeding rate, and weight gain of the gram pod borer.
Article
Agronomy
Meenal Rathore, Ayushi Tripathi, N. S. Kushwah, N. P. Singh
Summary: A rapid and precise genetic transformation tool, in vitro multiple shoot regeneration system, was successfully demonstrated in greengram using detached embryonic axis (EA) and double cotyledonary node (DCN) as explants. BAP was found to be the most effective hormone for inducing and regenerating multiple shoots. Rhizogenesis was achieved in EA explants on basal media without phytohormones and in DCN explants with 1.0 mg l-1 IAA. In vitro regenerated plantlets were hardened and produced flowers, pods, and viable seeds. This study revealed the potential of EA as an explant for in vitro regeneration in the cultivar SML668.
Article
Multidisciplinary Sciences
Rajesh Kumar Mishra, Vinod Kumar Mishra
Summary: In today's world, some countries are focused on reducing carbon emissions, and considering carbon emissions in inventory models is crucial for sustainable business practices. Quality products and environmental performance are key concerns in modern competitive businesses, with efforts being made to reduce emissions and maintain product quality through sustainable inventory planning.
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
(2022)
Article
Agronomy
Satheesh S. J. Naik, Amrit Lamichaney, Abhishek Bohra, R. K. Mishra, Farindra Singh, Dibendu Datta, I. P. Singh, N. P. Singh
Summary: The study identified pigeonpea genotypes with resistance to bruchid infestation, which can help reduce post-harvest losses.
Article
Mycology
Khela Ram Soren, Sandhya Tripathi, Shalini Pareek, Maloti Hembram, Priyanka Gangwar, Shrutika Abrol, Abhishek Bohra, Kuldeep Kumar, Aravind K. Konda, P. S. Shanmugavadivel, Md. Akram, Raj K. Mishra, Narendra Pratap Singh
FRONTIERS IN FUNGAL BIOLOGY
(2021)