期刊
CYTOSKELETON
卷 72, 期 7, 页码 362-371出版社
WILEY-BLACKWELL
DOI: 10.1002/cm.21233
关键词
Arp2; 3; actin cytoskeleton; migration; Rac; feedback loop
类别
资金
- Agence Nationale pour la Recherche [ANR-11-BSV8-0010-02]
- Institut National du Cancer [INCA_6521]
- Fondation ARC pour la Recherche sur le Cancer [PDF20111204331, PGA120140200831]
- Agence Nationale de la Recherche (ANR) [ANR-11-BSV8-0010] Funding Source: Agence Nationale de la Recherche (ANR)
Branched actin networks generated by the Arp2/3 complex provide the driving force for leading edge protrusion in migrating cells. We recently identified Arpin, a protein that inhibits the Arp2/3 complex in lamellipodia. Arpin is activated by the small GTPase Rac, which triggers lamellipodium formation, and thus Arpin renders protrusions unstable. A conserved role of Arpin is to induce migrating cells to turn in different migration models. Here we investigated the mechanism by which Arpin controls directional persistence. For this analysis, we segmented migration trajectories into alternating phases of active migration and pauses, based on a speed threshold. Regardless of the threshold value, Arpin induced more frequent pausing, during which the cell was more likely to change the direction of its migration. Arpin simultaneously acts on cell speed and directional persistence, which are strongly coupled parameters. Induction of frequent pausing by Arpin is consistent with Arpin circuitry: by inhibiting the Arp2/3 complex as a response to Rac activation, Arpin antagonizes a positive feedback loop that sustains protrusions at the leading edge and maintains active migration. We propose the duration of active migration' as a useful proxy to measure feedbacks associated with cell migration. (c) 2015 Wiley Periodicals, Inc.
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