Review
Biotechnology & Applied Microbiology
Liang Zhang, Xinlin Liang, Tomas Takac, George Komis, Xiaojuan Li, Yuan Zhang, Miroslav Ovecka, Yanmei Chen, Jozef Samaj
Summary: In plants, membrane compartmentalization relies on vesicle trafficking for organelle communication. Membrane proteins involved in vesicle trafficking are dynamic and responsive to environmental changes and cellular signals. Therefore, understanding the localization and dynamics of these proteins is crucial for dissecting vesicular trafficking pathways.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Optics
Chen Fan, Hong Zhao, Zixin Zhao, Junxiang LI, Yijun DU, Xingyu Yang, Lu Zhang
Summary: We propose a novel single-shot quantitative phase imaging technique using a liquid crystal spatial light modulator (LC-SLM) under white light illumination. By studying the phase modulation characteristics of an LC-SLM under white light illumination, images captured at different wavelengths are equivalent to those captured at different defocus distances. The phase is retrieved from a single-shot color image using the transport of intensity equation. The proposed method shows flexibility and accuracy in quantitative phase imaging of various samples.
Article
Multidisciplinary Sciences
Marco Leonetti, Lorenzo Pattelli, Simone De Panfilis, Diederik S. Wiersma, Giancarlo Ruocco
Summary: Through directly measuring spatially resolved intensity correlations of light inside a disordered medium using DNA strings decorated with emitters, researchers uncovered deviations in the size and polarization degrees of freedom of bulk speckles from theoretical predictions. These deviations are explained by correlations among polarization components and non-universal near-field contributions at the nanoscale.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Corentin Mollier, Joanna Skrzyde, Dorota Borowska-Wykre, Mateusz Majda, Vincent Bayle, Virginie Battu, Jean-Chrisologue Totozafy, Mateusz Dulski, Antoine Fruleux, Roman Wrzalik, Gregory Mouille, Richard S. Smith, Francoise Moneger, Dorota Kwiatkowska, Arezki Boudaoud
Summary: Extracellular matrices contain parallel fibril-like polymers, but it is still unclear how the subcellular control of fibril synthesis affects organ shape. In this study, we used Arabidopsis sepals as a model organ to investigate the role of CELLULOSE SYNTHASE INTERACTIVE1 (CSI1) in sepal morphogenesis. We found that csi1 mutant sepals are shorter with more aligned cellulose microfibrils compared to wild type, but their cell growth anisotropy remains similar. Our results suggest that CSI1 is required for spatial consistency of growth direction in sepals.
Article
Biochemical Research Methods
Kevin M. Dean, Tonmoy Chakraborty, Stephan Daetwyler, Jinlong Lin, Gerard Garrelts, Ons M'Saad, Hannahmariam T. Mekbib, Fabian F. Voigt, Martina Schaettin, Esther T. Stoeckli, Fritjof Helmchen, Joerg Bewersdorf, Reto Fiolka
Summary: The protocol provides detailed instructions for assembling and operating a versatile variant of light-sheet fluorescence microscopy called axially swept light-sheet microscopy (ASLM), which offers an unparalleled combination of field of view, optical resolution, and optical sectioning. It includes information on the working principle, applications, practical tips, part lists, schematics, and software for instrument control of ASLM.
Article
Multidisciplinary Sciences
Azeem Ahmad, Vishesh Dubey, Nikhil Jayakumar, Anowarul Habib, Ankit Butola, Mona Nystad, Ganesh Acharya, Purusotam Basnet, Dalip Singh Mehta, Balpreet Singh Ahluwalia
Summary: High space-bandwidth product and spatial phase sensitivity are crucial for single-shot quantitative phase microscopy (QPM) systems. The use of pseudo-thermal light source (PTLS) overcomes the limitations of traditional light sources in QPM, enabling better performance. PTLS achieves spatial phase sensitivity equivalent to white light sources and supports a field of view equivalent to laser sources, making it suitable for wider applications in life sciences and clinical settings.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Mythreye Venkatesan, Nicholas Zhang, Benoit Marteau, Yukina Yajima, Nerea Ortiz De Zarate Garcia, Zhou Fang, Thomas Hu, Shuangyi Cai, Adam Ford, Harrison Olszewski, Andrew Borst, Ahmet F. Coskun
Summary: Organelles play important roles in human health and disease by maintaining homeostasis, regulating growth and aging, and generating energy. Studying the distribution of organelles at the single-cell level is crucial for understanding cellular function. In this study, rapid multiplexed immunofluorescence (RapMIF) was used to investigate the spatial organization of organelle proteins in mesenchymal stem cells (MSCs) from bone marrow (BM) and umbilical cord (UC). The results showed that UC MSCs exhibited higher organelle expression and spatially spread distribution of mitochondria compared to BM MSCs, providing insights into the interrelations between organelles and the characteristics of MSC subtypes.
SCIENTIFIC REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Daniel Boening, Franz-Ferdinand Wieser, Vahid Sandoghdar
Summary: Super-resolution localization microscopy is achieved by determining the positions of individual fluorescent markers in a sample, with the challenge of limited photon collection from single emitters. Overcoming this challenge involves exploiting increased photostability at low temperatures and activating individual emitters, as well as utilizing emission polarization to access molecular orientation and aid in assigning photons to blinking molecules. Additionally, periodic modulation of excitation polarization serves as an effective method for switching fluorophores, as demonstrated in resolving two emitters on different DNA origami structures.
Review
Optics
Xi Chen, Mikhail E. Kandel, Gabriel Popescu
Summary: This paper introduces the principles, imaging mechanisms, operational requirements, and applications of Spatial Light Interference Microscopy (SLIM) in basic science and clinical research. SLIM is a highly sensitive quantitative phase imaging method that can assist in studying cell dynamics, cancer research, and more.
ADVANCES IN OPTICS AND PHOTONICS
(2021)
News Item
Optics
Stefan Scheel, Alexander Szameit
Summary: Non-Abelian braiding, crucial for achieving topological quantum computation, is realized using an array of photonic integrated waveguides.
Article
Biochemical Research Methods
Chad M. Hobson, Min Guo, Harshad D. Vishwasrao, Yicong Wu, Hari Shroff, Teng-Leong Chew
Summary: This Perspective discusses common pitfalls and provides guidance for improving quantitative imaging with light sheet microscopy. Fluorescence microscopy has transitioned into a platform for quantitative, hypothesis-driven research, leading to the rapid growth of light sheet fluorescence microscopy. The unique characteristics of light sheet microscopes require special considerations for quantitative experiments.
Article
Neurosciences
Mohammad A. A. Fakhree, Irene B. M. Konings, Jeroen Kole, Alessandra Cambi, Christian Blum, Mireille M. A. E. Claessens
Summary: This study reveals the relationship between alpha-synuclein and the endocytic pathway, showing a structural association of alpha S with caveolin-dependent membrane vesiculation further along the endocytic pathway, possibly linking alpha S function to vesicle trafficking dysfunction in Parkinson's disease.
Article
Optics
Mingyuan Hong, Ashe Miller, Roberto de J. Leon-Montiel, Chenglong You, Omar S. Magana-Loaiza
Summary: The study demonstrates the production of light beams with various photon statistics through the spatial modulation of coherent light, allowing for the shaping of spatial light modes with engineered photon statistics at different spatial positions.
LASER & PHOTONICS REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Christopher Halkias, Antony Orth, Bryce N. Feltis, Theodore A. Macrides, Brant C. Gibson, Paul F. A. Wright
Summary: The in vitro human THP‐1 monocyte model of cholesterol crystallization provides an effective and efficient means of quantitating cholesterol crystallization in the pre-clinical stage of research, and allows for the screening of potentially therapeutic compounds that may be used in attenuating or preventing cholesterol crystallization.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Multidisciplinary Sciences
Hadrien M. L. Robert, Kristyna Holanova, Lukasz Bujak, Milan Vala, Verena Henrichs, Zdenek Lansky, Marek Piliarik
Summary: The study introduces a high-speed photothermal spatial light modulator capable of generating a step-like wavefront change without diffraction artifacts, used for quantitative phase imaging to capture nanometer-scale 3D displacements.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Mayeul Collot, Emmanuel Boutant, Kyong Tkhe Fam, Lydia Danglot, Andrey S. Klymchenko
BIOCONJUGATE CHEMISTRY
(2020)
Article
Cell Biology
Alessandra Gallo, Lydia Danglot, Francesca Giordano, Bailey Hewlett, Thomas Binz, Christian Vannier, Thierry Galli
JOURNAL OF CELL SCIENCE
(2020)
Review
Cell Biology
Thomas Feaugas, Nathalie Sauvonnet
Summary: Infectious diseases are still a hot topic in research, with recent advancements in organ-on-chip technology showing great promise in recreating tissue physiology and studying host-pathogen interactions. This review highlights how this technology replicates tissue architecture and environment, playing a critical role in understanding the mechanisms of host-pathogen interactions.
CELLULAR MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Laura Salavessa, Thibault Lagache, Valerie Malarde, Alexandre Grassart, Jean-Christophe Olivo-Marin, Alexis Canette, Michael Trichet, Philippe J. Sansonetti, Nathalie Sauvonnet
Summary: This study investigates the clustering of IL-2 receptor gamma in endocytosis and its importance for signal transduction. Three distinct populations of IL-2R gamma clusters were identified, with different sizes playing different roles in IL-2R internalization and signaling. Membrane cholesterol and the branched actin cytoskeleton were found to be key regulators of IL-2R gamma clustering and IL-2-induced signaling, affecting CIE initiation and signal transduction.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
R. Sikora, P. Bun, L. Danglot, M. Alqabandi, P. Bassereau, F. Niedergang, T. Galli, A. Zahraoui
Summary: Secreted proteins are transported along intracellular route from the endoplasmic reticulum through the Golgi before reaching the plasma membrane. Small GTPase Rab and their effectors play a key role in membrane trafficking. MICAL-L1 is found to be associated with tubulo-vesicular structures and to have a significant colocalization with markers of the Golgi apparatus and recycling endosomes, revealing a new role in cargo delivery to the plasma membrane.
Article
Biochemistry & Molecular Biology
Meetali Singh, Maxime Chazal, Piergiuseppe Quarato, Loan Bourdon, Christophe Malabat, Thomas Vallet, Marco Vignuzzi, Sylvie van der Werf, Sylvie Behillil, Flora Donati, Nathalie Sauvonnet, Giulia Nigro, Maryline Bourgine, Nolwenn Jouvenet, Germano Cecere
Summary: A virus-derived miRNA produced during SARS-CoV-2 infection can target interferon-stimulated genes and suppress their expression, potentially aiding the virus in evading the host immune response. Detection of these viral miRNA isoforms in COVID-19 patients' samples suggests a mechanism by which the virus hijacks the host miRNA machinery.
Article
Multidisciplinary Sciences
Hela Benaissa, Karim Ounoughi, Isabelle Aujard, Evelyne Fischer, Rosette Goiame, Julie Nguyen, Alison G. Tebo, Chenge Li, Thomas Le Saux, Giulia Bertolin, Marc Tramier, Lydia Danglot, Nicolas Pietrancosta, Xavier Morin, Ludovic Jullien, Arnaud Gautier
Summary: Fluorescent reporters covering the visible spectrum are essential for imaging living cells and organisms. The authors have developed a collection of fluorogenic chromophores spanning from blue to red using a single engineered protein tag. This tool allows for tuning fluorescence color and properties, providing versatility for imaging proteins in live cells and optimizing FRET biosensors.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Sophie Michelis, Lydia Danglot, Romain Vauchelles, Andrey S. Klymchenko, Mayeul Collot
Summary: Tracking the pH variation of intracellular vesicles is crucial for assessing cell trafficking and metabolism. we designed a fluorogenic ratiometric pH probe, Mem-pH, which specifically targets the plasma membrane of cells and accumulates in intracellular vesicles through endocytosis. It allows for dynamic observation of vesicular acidification throughout the endocytic pathway and pH measurement through ratiometric imaging.
ANALYTICAL CHEMISTRY
(2022)
Article
Biology
Fanny Momboisse, Giacomo Nardi, Philippe Colin, Melanie Hery, Nelia Cordeiro, Simon Blachier, Olivier Schwartz, Fernando Arenzana-Seisdedos, Nathalie Sauvonnet, Jean-Christophe Olivio-Marin, Bernard Lagane, Thibault Lagache, Anne Brelot
Summary: This study investigated the impact of different states on the biological function and therapeutic targeting of CC chemokine receptor 5 (CCR5). The researchers used microscopy and statistical methods to track and classify the motion of different receptor subpopulations. The results showed that CCR5 exists in various ligand-free forms, stabilized by different ligands. Agonist stimulation restricted the mobility of CCR5 and led to its clustering, while inverse agonist stimulation had the opposite effect. The study also demonstrated the importance of CCR5 dimerization in its coupling to G proteins, immobilization and clustering upon activation, and receptor endocytosis.
Article
Multidisciplinary Sciences
Aleix Boquet-Pujadas, Thomas Feaugas, Alba Petracchini, Alexandre Grassart, Heloise Mary, Maria Manich, Samy Gobaa, Jean-Christophe Olivo-Marin, Nathalie Sauvonnet, Elisabeth Labruyere
Summary: This study reveals the significant role of mechanical cues in the invasion of enteropathogens and highlights the acceleration of tissue destruction and invasion by peristalsis. The activation of virulence genes in bacteria and facilitation of parasite penetration were also observed under local tension. This work introduces a framework for studying mechanobiology on deformable tissues.
Article
Chemistry, Multidisciplinary
Lazare Saladin, Victor Breton, Ophelie Dal Pra, Andrey S. S. Klymchenko, Lydia Danglot, Pascal Didier, Mayeul Collot
Summary: We introduce a new concept called Directed Photooxidation Induced Conversion (DPIC) to produce dual-color photoconvertible probes. Styryl-coumarins (SCs) with Aromatic Singlet Oxygen Reactive Moieties (ASORMs) have been synthesized to support this mechanism. SC-P, a yellow emitting probe with a pyrrole moiety, can convert to a stable blue emitting coumarin with a 68 nm shift, enabling photoconversion and tracking of lipid droplets in live cells. This novel approach may lead to a new generation of photoconvertible dyes for advanced bioimaging applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Clement Bonamy, Sabrina Pesnel, Maroua Ben Haddada, Olivier Gorgette, Christine Schmitt, Anne-Laure Morel, Nathalie Sauvonnet
Summary: Skin cancer, including melanoma and nonmelanoma cancers, is a global health issue. This study focuses on basal cell carcinoma as a good prognosis skin cancer for the first clinical proof of concept. Photothermal therapy using gold nanoparticles synthesized by green chemistry (gGNPs) is investigated for its potential to treat skin cancers. The study demonstrates that gGNPs are less cytotoxic than gold nanoparticles synthesized by Turkevich's method and optimized gGNP coating and stabilization are described. Glucose-stabilized gGNPs (gGNP@G) show specific internalization in cancerous cells with no cytotoxicity, providing a promising approach for targeted cancer treatment.
Article
Cell Biology
Natacha Zanin, Christine Viaris de Lesegno, Joanna Podkalicka, Thomas Meyer, Pamela Gonzalez Troncoso, Philippe Bun, Lydia Danglot, Daniela Chmiest, Sylvie Urbe, Jacob Piehler, Cedric M. Blouin, Christophe Lamaze
Summary: The involvement of IFN-α and β receptors in endosomal trafficking is crucial for the activation of JAK-STAT signaling pathway. STAM and Hrs play distinct roles in this process, leading to the differential regulation of JAK-STAT signaling by IFN-α and IFN-β.
NATURE CELL BIOLOGY
(2023)
Proceedings Paper
Engineering, Biomedical
Sammit Jain, Suvadip Mukherjee, Lydia Danglot, Jean-Christophe Olivo-Marin
Summary: This study presents an unsupervised path prediction technique based on a stochastic framework for reconstructing dendritic spines from fluorescent microscopy images. Experimental analyses show that the method significantly improves the reconstruction error.
2021 IEEE 18TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI)
(2021)
Article
Engineering, Electrical & Electronic
Suvadip Mukherjee, Catalina Gonzalez-Gomez, Lydia Danglot, Thibault Lagache, Jean-Christophe Olivo-Marin
IEEE SIGNAL PROCESSING LETTERS
(2020)
