期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 14, 页码 3013-3018出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b00304
关键词
tea; gamma-aminobutyric acid; GABA shunt; polyamine degradation; anoxic treatment
资金
- Natural Science Fund of Jiangsu Province [BK20140714]
- National Natural Science Foundation of China [31400584]
- China Postdoctoral Science Foundation [2015M581810]
- Research Fund for the Post Doctoral Project of Jiangsu Province [1501134C]
gamma-Aminobutyric acid (GABA) is an important bioactive component of tea (Camellia sinensis) providing various health benefits. We studied GABA accumulation-via the GABA shunt and polyamine degradation pathways under anoxia in tea leaves. Anoxia caused a similar to 20-fold increment in GABA concentration, relative to fresh tea leaves. This increment was due to the increase of glutamate decarboxylase and diamine oxidase activities. Genes involved in GABA formation, such as CsGAD1 and CsGAD2, were significantly up-regulated by anoxia. The concentrations of putrescine and spermine, two substrates for GABA production, were also increased by anoxia. Treating tea leaves with aminoguanidine completely inhibited diamine oxidase activity during anoxia, but the concentration of GABA decreased by only similar to 25%. We infer that about one-fourth of GABA formed in tea leaves under anoxia comes from the polyamine degradation pathway, opening the possibility of producing GABA tea based through the regulation of metabolism.
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