期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 23, 页码 4683-4690出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b00629
关键词
ganoderma; ganoderic acid; squalene epoxidase; fermentation; genetic engineering; co-overexpression
资金
- National Natural Science Foundation of China [31360495, 21566016]
The squalene epoxidase (SE) gene from the biosynthetic pathway of ganoderic acid (GA) was cloned and overexpressed in Ganoderma lingzhi. The strain that overexpressed the SE produced approximately 2 times more GA molecules than the wild-type (WT) strain. Moreover, SE overexpression upreplated lanosterol synthase gene expression in the biosynthetic pathway. These results indicated that SE stimulates GA accumulation. Then, the SE and 3-hydroxy-3-methylglutatyl coenzyme A (HMGR) genes were simultaneously overexpressed in G. lingzhi. Compared with the individual overexpression of SE or HMGR, the combined overexpression of the two, genes further enhanced individual GA, production. The overexpressing Strain produced maximum GA-T, GA-S) GA-Mk, and GA-Me contents of 90.4 +/- 7.5, 35.9 +/- 5.4, 61 +/- 0.5) and 61.8 +/- 5.8 mu g/100 mg dry weight, respectively. These valises were 5.9, 4.5, 2.4, and.5.8 times higher than those produced by the WT strain. This is the first example of the successful manipulation of multiple biosynthetic genes to improve GA content in G. lingzhi.
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