期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 65, 期 35, 页码 7624-7628出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b02255
关键词
cytokinin; abscisic acid (ABA); imaging mass spectrometry (WS); matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI -TOF-MS); trans-zeatin (tZ)
资金
- Japan Society for the Promotion of Science (JSPS) [JP 16K04890]
- Research and Development Foundation of the Hokuriku Industrial Advancement Center
- Grants-in-Aid for Scientific Research [16K04890] Funding Source: KAKEN
Plant hormones act as important signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Because their concentrations of hormones control the physiological responses in the target tissue, it is important to know the distributions and concentrations in the tissues. However, it is difficult to determine the hormone concentration on the plant tissue as a result of the limitations of conventional methods. Here, we report the first multi-imaging of two plant hormones, one of cytokinin [i.e., trans-zeatin (tZ)] and abscisic acid (ABA) using a new technology, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) imaging. Protonated signals of tZ (m/z 220.1) and ABA (m/z 265.3) were chosen on longitudinal sections of rice roots for MS imaging. tZ was broadly distributed about 40 mm behind the root apex but was barely detectable at the apex, whereas ABA was mainly detected at the root apex. Multi imaging using MALDI TOF MS enabled the visualization of the localization and quantification of plant hormones. Thus, this tool is applicable to a wide range of plant species growing under various environmental conditions.
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