Review
Microbiology
Francis Muchaamba, Karen Barmettler, Andrea Treier, Kurt Houf, Roger Stephan
Summary: Escherichia albertii is an emerging zoonotic foodborne pathogen associated with watery diarrhea, mainly in children and immunocompromised individuals. It can be transmitted through contaminated water and food, especially poultry-based products. Due to the lack of standardized identification protocols, it can be easily misidentified as other Enterobacteriaceae. Therefore, simple and efficient diagnostic tools are needed for accurate identification of this elusive pathogen.
Article
Microbiology
Huiwen Wang, Li Zhang, Liu Cao, Ximin Zeng, Barbara Gillespie, Jun Lin
Summary: This study found that Escherichia albertii is prevalent in chicken farms in the United States, displaying various antibiotic resistance and showing close evolutionary relationships with human strains. The findings highlight the importance of preventing and controlling E. albertii in poultry production.
VETERINARY MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Atsushi Hinenoya, Xing-Ping Li, Ximin Zeng, Orhan Sahin, Rodney A. Moxley, Catherine M. Logue, Barbara Gillespie, Shinji Yamasaki, Jun Lin
Summary: This study investigated the prevalence and characteristics of E. albertii in US domestic and food animals, revealing chickens as an important reservoir for E. albertii. The isolated chicken E. albertii strains displayed multidrug resistance and phylogenetic closeness to human-derived strains. Further large-scale surveys in poultry production are warranted in the future.
ZOONOSES AND PUBLIC HEALTH
(2021)
Article
Genetics & Heredity
Katarzyna Leszczynska, Izabela Swiecicka, Tamara Daniluk, Dariusz Lebensztejn, Sylwia Chmielewska-Deptula, Dorota Leszczynska, Jan Gawor, Malgorzata Kliber
Summary: The study assessed the prevalence and pathogenicity of E. albertii strains in northeastern Poland and found high genetic variability and potential public health threat.
Article
Infectious Diseases
Gillian A. M. Tarr, Joshua Rounds, Madhura S. Vachon, Kirk Smith, Carlota Medus, Craig W. Hedberg
Summary: This study examines the incidence and risk factors of different serogroups of STEC infections in Minnesota. The results indicate variations in transmission routes and risk factors among different serogroups, suggesting the need for serogroup-specific disease control strategies.
JOURNAL OF INFECTION
(2023)
Article
Biotechnology & Applied Microbiology
Yuki Wakabayashi, Kazuko Seto, Masashi Kanki, Tetsuya Harada, Kentaro Kawatsu
Summary: In this study, a selective enrichment broth, novobiocin-cefixime-tellurite supplemented modified tryptic soy broth (NCT-mTSB), was developed to selectively enrich and isolate Escherichia albertii from poultry samples. This new method showed superior results compared to traditional enrichment broths and was helpful for the isolation of this emerging diarrheagenic pathogen.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Michelle Qiu Carter, Beatriz Quinones, Xiaohua He, Antares Pham, Diana Carychao, Michael B. Cooley, Chien-Chi Lo, Patrick S. G. Chain, Rebecca L. Lindsey, James L. Bono
Summary: Through comparative genomics and phenotypic characterization, this study assessed the pathogenic potential of Escherichia albertii strains isolated from wild birds in California. The results showed that these strains have high pathogenicity potential and could cause severe diseases in humans.
Article
Biotechnology & Applied Microbiology
Bingting Xu, Noritoshi Hatanaka, Sharda Prasad Awasthi, Keiji Tekehira, Atsushi Hinenoya, Shinji Yamasaki
Summary: The aim of this study was to develop a selective enrichment broth for efficient isolation of Escherichia albertii. A total of 412 raccoon rectal swabs were tested to quantify the number of E. albertii, and its isolation rate was greatly affected by the number of bacteria in the sample. The selective medium, CTD-TSB, improved the isolation efficiency of E. albertii in PBS suspension containing raccoon feces.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Food Science & Technology
Emmanuel W. Bumunang, Rahat Zaheer, Kim Stanford, Chad Laing, Dongyan Niu, Le Luo Guan, Linda Chui, Gillian A. M. Tarr, Tim A. McAllister
Summary: This study characterized the presence of Shiga toxin-producing Escherichia coli (STEC) O157 in cattle and clinical samples through a comparative genomic analysis. It was found that most isolates carried virulence genes stx1, stx2, eae, and hlyA, with variations in stx-phage insertion sites and antimicrobial resistance genes between cattle and clinical strains.
Article
Microbiology
Rajal K. Mody, Robert M. Hoekstra, Magdalena Kendall Scott, John Dunn, Kirk Smith, Melissa Tobin-D'Angelo, Beletshachew Shiferaw, Katie Wymore, Paula Clogher, Amanda Palmer, Nicole Comstock, Kari Burzlaff, Sarah Lathrop, Sharon Hurd, Patricia M. Griffin
Summary: The study found that there is a risk of developing HUS in patients with O157 diarrhea who are treated with beta-lactam antibiotics, especially in children and adults. Macrolide antibiotics seem to have a protective effect against HUS, although not statistically significant. Overall, there was no significant association between antimicrobial use and the risk of HUS.
Article
Microbiology
Betul Umay, Abdulkadir Gul, Muhammet Saban Tanyildizi
Summary: This study focused on the isolation, identification, and optimization of fibrinolytic protease-producing strains using fermentative protein sources. Fibrinolytic protease-producing strains were selected through analysis on skim milk agar medium, and the strain with the highest activity was identified as Acinetobacter johnsonii TR01 by 16S rRNA analysis. The optimal conditions for maximum fibrinolytic protease production were determined to be at 60 degrees C and pH 7.0, with fructose as the best carbon source and yeast extract and peptone as the best nitrogen sources. Response Surface Methodology was used to determine the medium composition for maximum enzyme activity (8.30 IU/ml), which included 14.22 g/L fructose, 11.190 g/L yeast extract, 14.22 g/L peptone, 0.5 g/L MnCl2, and 0.5 g/L ZnC4H6O4.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Biology
Khurshid Jalal, Kanwal Khan, Ajmal Hayat, Sulaiman Mohammed Alnasser, Alotaibi Meshal, Zarrin Basharat
Summary: In this study, a comprehensive in silico strategy was used to identify antibiotic-resistant genes and drug targets from E. albertii genomes. The essential cell division protein ZipA was selected for further processing, and the compound ZINC85624912 from a Chinese medicinal library showed the highest bioavailability and plasma retention. This study provides valuable information for the development of drug-like compounds from natural resources against antibiotic-resistant E. albertii.
Article
Immunology
David A. Montero, Richard Garcia-Betancourt, Roberto M. Vidal, Juliana Velasco, Pablo A. Palacios, Daniela Schneider, Carolina Vega, Leonardo Gomez, Hernan Montecinos, Rodrigo Soto-Shara, Angel Onate, Leandro J. Carreno
Summary: Researchers have developed a chimeric protein-based vaccine targeting seven virulence factors of Shiga toxin-producing Escherichia coli (STEC), which elicited significant immune responses in mice and provided protection against infection. However, further improvement is needed to enhance the vaccine's efficacy and explore other potential mechanisms of protection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Medicinal
Diksha Haksar, Mostafa Asadpoor, Torben Heise, Jie Shi, Saskia Braber, Gert Folkerts, Lluis Ballell, Janneth Rodrigues, Roland J. Pieters
Summary: In this study, three classes of carbohydrate-based inhibitors were compared to find a simple and effective toxin inhibitor. Multivalent inhibitors showed enhanced potency, with the polymeric inhibitor based on galabiose exhibiting the most potent inhibition. Alginate and chitosan oligosaccharides were also effective in inhibiting Shiga toxin and could be used as prophylactic drugs during shigella outbreaks.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Food Science & Technology
Chi Chen, Yue Yue, Zhujun He, Bowen Li, Jianxin Zhao, Wei Chen, Bo Yang
Summary: This study compared the effects of five strains of Lactobacillus gasseri on ETEC-induced diarrhea and investigated their mechanisms. The results showed that three strains of L. gasseri could alleviate diarrhea by reducing inflammatory factors, normalizing gut microbiota, and producing short-chain fatty acids (SCFAs).