Article
Immunology
Yussuf Ali, Ibrahim Inusa, Gaurav Sanghvi, Viralkumar B. Mandaliya, Ashok Kumar Bishoyi
Summary: Phage therapy has the advantages of eliminating multidrug-resistant pathogens without harming beneficial microbiota, making it a novel approach for combating resistant pathogens. Although it lacks complete clinical data, clinical studies have shown its safety and efficacy in some cases, alleviating concerns. The development of phage therapeutics is progressing smoothly, and gene-editing tools like CRISPR-Cas allow the engineering of phages to maximize treatment effectiveness.
MICROBIAL PATHOGENESIS
(2023)
Review
Biotechnology & Applied Microbiology
Mohadeseh Hassannia, Mahin Naderifar, Shakiba Salamy, Majid Reza Akbarizadeh, Samane Mohebi, Majid Taati Moghadam
Summary: In recent decades, the rapid expansion of drug-resistant bacteria has become a serious health concern. This review focuses on the use of genetically engineered phage enzymes (EPE) as a potential solution. Evidence shows that EPE has superior performance in dealing with antibiotic-resistant infections compared to natural endolysins and phages.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Review
Pharmacology & Pharmacy
Rachel Yoon Kyung Chang, Sue C. Nang, Hak-Kim Chan, Jian Li
Summary: The rise of antibiotic resistance has made antibiotic therapy ineffective against bacterial infections. ESKAPE pathogens, which exhibit high levels of resistance, pose a major global health threat. Novel antimicrobial therapeutic strategies, including bacteriophages, enzymes, immunomodulators, and monoclonal antibodies, are urgently needed to address this issue.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Microbiology
Avijit Goswami, Pallavi Raj Sharma, Rachit Agarwal
Summary: Intracellular pathogens pose challenges for antibiotic delivery, leading to exploration of phage therapy as an alternative. While phage therapy has been used for bacterial infections, its efficacy against intracellular bacteria is still being investigated.
CRITICAL REVIEWS IN MICROBIOLOGY
(2021)
Review
Microbiology
Mark B. Carascal, Donna May dela Cruz-Papa, Roland Remenyi, Mely Cherrylynne B. Cruz, Raul V. Destura
Summary: Southeast Asia is a hotspot for antimicrobial resistance, and phage therapy has emerged as a promising alternative for treating multidrug-resistant infections. This narrative review highlights the current understanding of phages and presents a three-phase strategy to encourage a phage revolution in the clinical setting of Southeast Asia. The goal is to open avenues for scientific and policy-based discussions on phage therapy and combat the threat of multidrug-resistant pathogens.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Infectious Diseases
Chengxi Liu, Qixuan Hong, Rachel Yoon Kyung Chang, Philip Chi Lip Kwok, Hak-Kim Chan
Summary: This review paper summarizes the progress of phage-antibiotic therapy, including the mechanisms of Phage-Antibiotic Synergy (PAS) and its therapeutic performance. The choice of phages and antibiotics, administration time and sequence, and concentrations of the two agents have different impacts on bacterial inhibitory effects.
Review
Infectious Diseases
Hyun Young Kim, Rachel Yoon Kyung Chang, Sandra Morales, Hak-Kim Chan
Summary: This paper reviews the formulation of phage-delivering hydrogels for orthopedic implant-associated bone infection, catheter-associated urinary tract infection, and trauma-associated wound infection, focusing on the preparation methods, stability, efficacy, and safety of hydrogels as phage carriers.
Article
Biochemistry & Molecular Biology
Natalia Kazmierczak, Bartlomiej Grygorcewicz, Marta Roszak, Beata Bochentyn, Lidia Piechowicz
Summary: The investigation and development of novel treatment strategies are necessary to address problems related to biofilm-related infections and antibiotic resistance. Bacteriophages, as promising alternatives to conventional antibiotics, have been shown to be effective antibiofilm agents in both in vitro and in vivo larva model studies. The results demonstrate that phages vB_SauM-A, vB_SauM-C, and vB_SauM-D can effectively reduce the colony-forming unit number of multidrug-resistant S. aureus strains in biofilms, independently of the phage titer used. Furthermore, bacteriophages were found to be more efficient than antibiotics in removing biofilm biomass and reducing staphylococci count. Additionally, phages vB_SauM-A, vB_SauM-C, and vB_SauM-D can enhance the survival rate and prolong the survival time of Galleria mellonella larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Haoju Huang, Wei Geng, Xizheng Wu, Yiyun Zhang, Lan Xie, Tian Ma, Chong Cheng
Summary: With the rise of antibiotic-resistant pathogens, new strategies for combating pathogenic bacteria are of great importance. In this study, an iron oxide-based spiky artificial peroxidase (POD) with V-O-Fe pair sites (V-Fe2O3) was created to combat methicillin-resistant Staphylococcus aureus (MRSA). Experimental studies and theoretical calculations demonstrated that the V-Fe2O3 can achieve localized capture and killing through its spiky morphology and production of massive reactive oxygen species (ROS). The V-Fe2O3 efficiently oxidized the lipid membrane, resulting in nearly 100% bacterial inhibition over a long period. Wound disinfection results showed that the V-Fe2O3 not only efficiently eliminated MRSA and their biofilm but also accelerated wound recovery without causing noticeable inflammation and toxicity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Medicine, Research & Experimental
Rahul Bhattacharjee, Aditya Nandi, Adrija Sinha, Hrithik Kumar, Disha Mitra, Abhik Mojumdar, Paritosh Patel, Ealisha Jha, Suman Mishra, Prabhat Kumar Rout, Pritam Kumar Panda, Mrutyunjay Suar, Suresh K. Verma
Summary: This review discusses the structural association, mechanism, and characterization of Phage Tail Like bacteriocins (PTLBs), as well as the symbiotic biological function and application of PTLBs against drug-resistant pathogens. The article also highlights the evolutionary role of PTLBs and the challenges that need to be addressed for clinical implementation.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Microbiology
Jun Luo, Libo Xie, Min Liu, Qianyuan Li, Peng Wang, Chunhua Luoa
Summary: Phage-antibiotic combination (PAC) therapy is a potential new alternative to treat infections caused by antibiotic-resistant bacteria. The combination of phage and antibiotics can produce synergistic effects, reduce antibiotic concentration, inhibit biofilm formation, and remove mature biofilms.
MICROBIOLOGY SPECTRUM
(2022)
Article
Materials Science, Multidisciplinary
Mert Tunca Doganay, Cyril John Chelliah, Abdullah Tozluyurt, Andrea M. Hujer, Stephen K. Obaro, Umut Gurkan, Robin Patel, Robert A. Bonomo, Mohamed Draz
Summary: Three-dimensional (3D) printing technology has significant potential for translational applications in biology and medicine. It can be used as a replacement for conventional solutions and to improve antimicrobial resistance (AMR). The technology allows for precise control of cells and their microenvironment, simulating complex in vivo environments and advancing the modeling and treatment of challenging infections and other health conditions. 3D printing also models the interactions between microbes and host-pathogens, specifically the development of antibiotic resistance. It can be applied to testing and delivering antibiotics.
Review
Infectious Diseases
Antonio Broncano-Lavado, Guillermo Santamaria-Corral, Jaime Esteban, Meritxell Garcia-Quintanilla
Summary: The increase in bacterial multiresistance and the shortage of new antibiotics are major public health concerns. Phage therapy has emerged as a promising alternative treatment, especially when used in combination with antibiotics to deal with infections caused by multidrug-resistant bacteria.
Article
Microbiology
Wattana Pelyuntha, Ruttayaporn Ngasaman, Mingkwan Yingkajorn, Kridda Chukiatsiri, Soottawat Benjakul, Kitiya Vongkamjan
Summary: This study evaluated an alternative strategy using phage-based treatment against Salmonella isolated from broiler production, showing a high prevalence of Salmonella spp. in bedding samples from broiler farms in eastern and southern Thailand. Multiple Salmonella phages were isolated from various water sources and found to effectively lyse several Salmonella serovars, leading to the conclusion that a phage cocktail can be an effective biocontrol method against Salmonella in broiler production and MDR serovars.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Virology
Tahir Farooq, Muhammad Dilshad Hussain, Muhammad Taimoor Shakeel, Muhammad Tariqjaveed, Muhammad Naveed Aslam, Syed Atif Hasan Naqvi, Rizwa Amjad, Yafei Tang, Xiaoman She, Zifu He
Summary: Plants are constantly threatened by severe microbial diseases, and the use of antibiotics and chemical-based bactericides to combat plant bacterial diseases has led to the emergence of resistant bacteria and negative impacts on the environment and human health. Phage cocktail therapy, on the other hand, is a multidimensional approach that effectively controls diverse resistant bacterial infections without harming the ecosystem. This review highlights the development and application of phage cocktails in combating resistant plant-bacterial pathogens and provides insights into recent advances in using phage cocktail therapy for the biocontrol of devastating plant diseases.