Article
Chemistry, Analytical
Uraiwan Kositanont, Chatchawan Srisawat, Sirinapa Sripinitchai, Charin Thawornkuno, Thanyarat Chaibun, Sinthu Karunaithas, Chamras Promptmas, Benchaporn Lertanantawong
Summary: Researchers developed an electrochemical aptasensor to detect the electron transfer flavoprotein subunit beta (ETFB) for diagnosing leptospiral infection.
Review
Chemistry, Analytical
Somayeh Vandghanooni, Zohreh Sanaat, Raheleh Farahzadi, Morteza Eskandani, Hossein Omidian, Yadollah Omidi
Summary: Early diagnosis of cancer is crucial, and aptamer-based biosensors have been developed as reliable tools for detecting various cancer markers, increasing the chances of successful treatment.
MICROCHEMICAL JOURNAL
(2021)
Article
Materials Science, Multidisciplinary
Avinash Sharma, Kanika Dulta, Rupak Nagraik, Kamal Dua, Sachin K. Singh, Dinesh K. Chellappan, Deepak Kumar, Dong-Soo Shin
Summary: Cancer is a deadly disease that affects people worldwide, with late-stage diagnosis being a major challenge. Developing early and sensitive cancer diagnostics is crucial, and aptasensors have shown promise as a potentially important tool.
Review
Medical Laboratory Technology
Esther Campos-Fernandez, Nathalia Oliveira Alqualo, Lorrayne Cristina Moura Garcia, Caroline Coutinho Horacio Alves, Tabatha Danielle Ferreira Arantes Vieira, Danilo Caixeta Moreira, Vivian Alonso-Goulart
Summary: Aptamers have the potential to be more effective diagnostic and therapeutic tools in the field of prostate cancer, particularly in blood-based liquid biopsies and electro-analytical methods. However, while they show clinical promise, the development of these strategies is still in its early stages.
CLINICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Po-Jung Jimmy Huang, Juewen Liu
Summary: In this study, DNA aptamers for L-lactate were obtained through library-immobilization selection method with high affinity and selectivity. A fluorescent biosensor based on the strand displacement method was constructed, allowing simultaneous detection of lactate and glucose in the same solution. This research expands the application of aptamers to simple metabolites and provides a useful tool for continuous and multiplexed monitoring.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Analytical
Somayeh Vandghanooni, Zohreh Sanaat, Jaleh Barar, Khosro Adibkia, Morteza Eskandani, Yadollah Omidi
Summary: The cost-efficient sensitive detection of solid tumor markers in patients' serums remains a significant challenge, but advancements in aptamer-based sensors and microfabricated microfluidic systems offer promising solutions. These technologies have allowed for the development of sophisticated sensors capable of detecting various molecular biomarkers of detrimental diseases, including ovarian cancer, with high accuracy and specificity.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Seungheon Lee, Shivudu Godhulayyagari, Shadler T. Nguyen, Jasmine K. Lu, Sasha B. Ebrahimi, Devleena Samanta
Summary: The use of DNA-based nanostructures as probes has led to significant advances in chemical and biological sensing, allowing the detection of analytes in complex media, the understanding of fundamental biological processes, and the ability to diagnose diseases based on molecular signatures.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Fangteng Song, Chao Wang, Chunhua Wang, Jianwei Gao, Hong Liu, Yu Zhang, Lin Han
Summary: This Feature discusses the working fundamentals, advanced strategies, and future outlook and challenges of enrichment-detection integrated biosensors for exosome profiling.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Morteza Banakar, Masoud Hamidi, Zohaib Khurshid, Muhammad Sohail Zafar, Janak Sapkota, Reza Azizian, Dinesh Rokaya
Summary: This review discusses the application of electrochemical biosensors in pathogen detection. The study comprehensively discusses transduction elements, biorecognition components, and electrode changes in electrochemical biosensors. It categorizes pathogen detection methods based on sample preparation and secondary binding processes, and highlights the potential benefits of electrochemical biosensors in medicine, environmental monitoring, and biothreat detection.
Article
Chemistry, Analytical
Ishak Afsin Kariper, Zafer Ustundag, Mustafa Oguzhan Caglayan
Summary: A sensitive and selective sensor based on aptamer and spectroscopic ellipsometry was developed for early diagnosis of breast cancer. The sensor achieved a detection range of VEGF165 from 1 pM to 1000 pM in buffer, with detection limits of 5.81 pM and 4.29 pM, respectively.
Review
Chemistry, Analytical
Vicente Antonio Miron-Merida, Yun Yun Gong, Francisco M. Goycoolea
Summary: This article discusses the issue of mycotoxin contamination, particularly fumonisin B1 (FB1), and the traditional and novel analytical methods used to quantify FB1. It introduces aptamers as a promising alternative method, explores specific aptamers for FB1, and emphasizes the potential of aptasensors for future development of highly sensitive FB1 testing methods.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Irene Palacio, Miguel Moreno, Almudena Nanez, Agnes Purwidyantri, Telma Domingues, Patricia D. Cabral, Jerome Borme, Marzia Marciello, Jesus Ignacio Mendieta-Moreno, Beatriz Torres-Vazquez, Jose Ignacio Martinez, Maria Francisca Lopez, Mar Garcia-Hernandez, Luis Vazquez, Pavel Jelinek, Pedro Alpuim, Carlos Briones, Jose Angel Martin-Gago
Summary: Biosensors based on covalently functionalized graphene have been developed for ultra-sensitive aptamer-based detection of hepatitis C virus core protein. The induced polarization at the graphene interface causes a net shift of the Dirac cone, enhancing the sensitivity for attomolar detection. This unexpected effect opens up possibilities for ultrasensitive and real-time diagnostics of various diseases using graphene-based functionalized platforms.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Haixiang Yu, Obtin Alkhamis, Juan Canoura, Yingzhu Liu, Yi Xiao
Summary: This review discusses the challenges and advances in isolating and characterizing small-molecule-binding DNA aptamers, including in vitro methodologies for isolation, strategies for optimal binding properties, techniques for characterizing aptamer-target binding and structure, and various small-molecule sensing platforms and detection applications based on aptamers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Claire Husser, Stephane Vuilleumier, Michael Ryckelynck
Summary: This paper introduces a RNA-based biosensor, FluorMango, which can specifically detect fluoride. The biosensor is composed of two RNA modules, one of which can change its structure to sense fluoride, while the other module emits fluorescence when bound to a fluorogen. The study illustrates the value and efficiency of this biosensor in directly monitoring defluorinase activity in bacterial cells.
Review
Chemistry, Analytical
Chenghua Zong, Xiaoting Jin, Juewen Liu
Summary: This article critically reviews the recent advances in arsenic detection using biomolecules and nanomaterials, discussing interactions between arsenic and gold, thiol, and DNA aptamers. It suggests that bio/nano systems show promise for arsenic detection and future research directions may include fundamental studies, searching for more specific arsenic binding materials and aptamers, incorporation of sensors into portable devices, and more systematic testing of sensors in real samples.
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Zeynep Yegin, Mediha Nur Zafer Yurt, Behiye Busra Tasbasi, Elif Esma Acar, Osman Altunbas, Samet Ucak, Veli Cengiz Ozalp, Mert Sudagidan
Summary: The bacterial microbiota of industrially produced kefir beverages consumed in Turkey was analyzed using a culture-independent and metagenomic approach. The dominant phyla were Firmicutes, followed by Actinobacteria and Proteobacteria. Lactococcus was the most abundant genus in non pre-enriched kefirs, while Streptococcus was dominant in pre-enriched kefirs. Several genera were only found in pre-enriched kefirs. The study suggests potential applications of kefir beverages in industrial and medical fields.
INTERNATIONAL DAIRY JOURNAL
(2022)
Article
Food Science & Technology
Samet Ucak, Mediha Nur Zafer Yurt, Behiye Busra Tasbasi, Elif Esma Acar, Osman Altunbas, Ali Soyucok, Ali Aydin, Veli Cengiz Ozalp, Mert Sudagidan
Summary: Metagenomic analysis revealed the bacterial microbiota of Boza samples, with a dominance of Lactococcus and Enterococcus after pre-enrichment. Clonal relationships, virulence properties, and antibiotic resistance of Enterococcus faecium strains in Boza were determined, highlighting the importance of microbial dynamics in fermentation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Elif Esra Altuner, Veli Cengiz Ozalp, M. Deniz Yilmaz, Muhammed Bekmezci, Fatih Sen
Summary: This study evaluated the analytical studies of Chitosan-Cobalt(II) nanoparticles and developed a selective TMB biosensor using catalytic reaction. The synthesized nanoparticles showed similar behavior to the peroxidase enzyme.
Article
Immunology
Mert Sudagidan, Veli Cengiz Ozalp, Ozge Can, Hakan Eligul, Mediha Nur Zafer Yurt, Behiye Busra Tasbasi, Elif Esma Acar, Murat Kavruk, Oner Kocak
Summary: This study investigated the surface microbiota of houseflies and identified the presence of Staphylococcus, Bacillus, and Enterococcus. Staphylococcus strains were selectively isolated and found to carry virulence genes and exhibit antibiotic resistance. Biofilm formation was observed in some strains, which played an important role in bacterial transmission on the surface of houseflies. The study sheds light on the composition and carriage of pathogenic staphylococci on housefly surfaces.
MICROBIAL PATHOGENESIS
(2022)
Article
Chemistry, Analytical
Ali D. Dursun, Baris A. Borsa, Gulay Bayramoglu, M. Yakup Arica, Veli C. Ozalp
Summary: A Surface Plasmon Resonance (SPR) aptasensor was developed for the sensitive detection of Brucella melitensis in milk samples. By using aptamers with high affinity and specificity, the aptasensor enables quick and efficient purification and detection of Brucella melitensis contamination in milk samples. The integration of a magnetic isolation step and SPR technology allows for instant and reliable detection of Brucella melitensis.
Article
Chemistry, Analytical
Bilge Guvenc Tuna, Dilara Buse Durdabak, Meltem Kazak Ercan, Soner Dogan, Murat Kavruk, Ali Dogan Dursun, Serap Demir Tekol, Caner Celik, Veli Cengiz Ozalp
Summary: This study presents a direct and fast detection method for the CoV-2 genome in nose-throat swab samples, without the need for further processing. The method utilizes fluorescein-loaded mesoporous silica nanoparticles with specific gene sequence probes on their surface, which are released upon hybridization with the target viral genome.
Article
Biochemistry & Molecular Biology
Esra Ersoy Omeroglu, Dilhun Keriman Arserim-Ucar, Zeynep Yegin, Nevzat Caglayan, Mediha Nur Zafer Yurt, Behiye Busra Tasbasi, Elif Esma Acar, Samet Ucak, Veli Cengiz Ozalp, Mert Sudagidan
Summary: PCR amplification and next-generation sequencing were utilized to identify the bacterial communities in propolis samples collected from Apis mellifera production hives in Eastern Turkiye. Firmicutes were found to be the dominant phylum, followed by Proteobacteria, Actinobacteria, Tenericutes, and Spirochaetes. Bacillaceae, Enterobacteriaceae, and Enterococcaceae were the top three bacterial families, with Bacillus being the dominant genus. Further investigation is needed to understand the microbiota of propolis and its potential applications from ecological, nutritional, and medicinal perspectives.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Food Science & Technology
Esra Ersoy Omeroglu, Oezge Can, Sevval Nur Temiz, Rabia Al, Osman Altunbas, Ali Soyucok, Mediha Nur Zafer Yurt, Veli Cengiz Ozalp, Mert Sudagidan
Summary: This study examined the bacterial microbiota and chemical properties of tarhana samples from Turkey. The dominant bacterial families were Bacillaceae, Enterococcaceae, Paenibacillaceae, Enterobacteriaceae, and Clostridiaceae, and the dominant genera were Bacillus, Enterococcus, and Paenibacillus. The chemical analysis showed variations in acidity, moisture content, and salt values among the tarhana samples.
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES
(2022)
Review
Chemistry, Analytical
Farzaneh Bonyadi, Murat Kavruk, Samet Ucak, Barbaros Cetin, Gulay Bayramoglu, Ali D. D. Dursun, Yakup Arica, Veli C. C. Ozalp
Summary: Continuous monitoring of pathogens has wide applications and Quartz crystal microbalance (QCM) is a promising real-time detection method. QCM biosensors, with high sensitivity and rapid detection, offer hope for early infection detection and disease tracking. This review provides an overview of QCM biosensing, summarizes notable examples of QCM biosensors for pathogens, and explores the use of QCM sensors in various samples and the integration of magnetic nanoparticles in automated detection.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Meeting Abstract
Physiology
Ali Dursun, Bilge Tuna, Nazim Keles, Soner Dogan, Mert Sudagidan, Ozlem Cetiner, Tugce Balci, Murat Kavruk, Veli Ozalp
Article
Food Science & Technology
Ali Aydin, Mert Sudagidan, Zhanylbubu Mamatova, Mediha Nur Zafer Yurt, Veli Cengiz Ozalp, Jacob Zornu, Saraya Tavornpanich, Edgar Brun
Summary: In this study, the presence of Pseudomonas in the skin swabs of seabass farmed in the Aegean Sea was analyzed and identified. A total of 46 viable Pseudomonas strains were isolated using conventional methods. Antibiotic susceptibility testing revealed that three strains were resistant to doripenem and two strains were resistant to imipenem. This data provides insight into the bacterial diversity and antibiotic resistance of psychrotrophic Pseudomonas in seabass from the Aegean Sea in Turkey.
Article
Pharmacology & Pharmacy
Veli Cengiz Ozalp, Samet Ucak, Ali D. Dursun, Mert Sudagidan, Oyku Icin, Cekdar Vakifahmetoglu, Laura M. Henning, Ulla Simon, Aleksander Gurlo
Summary: Different types of ordered mesoporous silica (OMS) nanoparticles, including COK-12 and modified COK-12, have been used as drug carriers for controlled drug adsorption and release. Cytotoxicity analysis showed that regular COK-12 particles had lower harm to mammalian cultured cells compared to other OMS nanoparticles. Regular COK-12 was further used as a carrier for a dual antibiotic-loaded drug delivery material, which showed enhanced antibiotic treatments against methicillin-resistant Staphylococcus strains through aptamer targeting.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Murat Kavruk, Samet Ucak, Burcu Sapmaz, Canan Cicek Demir, Ali Dogan Dursun
Summary: This study analyzed the total antibiotic consumption trends in the ATC group 101 in Turkey and European countries, and found that while Turkey showed a significant decrease in antibiotic consumption between 2010 and 2015, there was a deviation in the 2021 consumption data. In contrast, European countries experienced an overall decrease in antibiotic consumption during the COVID-19 pandemic.
FLORA INFEKSIYON HASTALIKLARI VE KLINIK MIKROBIYOLOJI DERGISI
(2023)
Article
Chemistry, Analytical
Ali Dogan Dursun, Soner Dogan, Murat Kavruk, B. Busra Tasbasi, Mert Sudagidan, M. Deniz Yilmaz, Bayram Yilmaz, Veli C. Ozalp, Bilge G. Tuna
Summary: A labelless biosensor based on surface plasmon resonance was developed to measure the concentration of Intercellular Adhesion Molecule-1 (ICAM-1) in blood samples. The sensor showed high specificity and low detection limit, providing real-time information on ICAM-1 protein concentration.