Article
Chemistry, Medicinal
Maxim S. Kokoulin, Lyudmila A. Romanenko, Aleksandra S. Kuzmich, Oleg Chernikov
Summary: Two cell-wall-associated polysaccharides were isolated and purified from a deep-sea marine bacterium, with the first one containing d-arabinose and the second one consisting of d-galactose and a rare component of bacterial glycans, d-xylulose.
Article
Multidisciplinary Sciences
Go Furusawa, Nor Azura Azami, Aik-Hong Teh
Summary: This study investigated the degradation of uronic acid-containing polysaccharides by the bacterium Catenovulum sp. CCB-QB4. The QB4 cells were able to utilize polysaccharides such as alginate as a carbon source, and the genome analysis revealed the presence of multiple polysaccharide lyases and glycoside hydrolases involved in the degradation process, highlighting the potential of QB4 as a source of novel genes for polysaccharide degradation.
Article
Ecology
Fang Qin, Sen Du, Zefeng Zhang, Hanqi Ying, Ying Wu, Guiyuan Zhao, Mingyu Yang, Yanlin Zhao
Summary: Viruses in the ocean have significant impacts on biogeochemical cycles, host mortality, population structure, physiology, and evolution. The HMO-2011-type viral group is dominant and widespread, but its genetic diversity, potential hosts, and ecology are poorly understood. This study identified and characterized multiple HMO-2011-type phages and highlighted their importance as major ecological agents targeting specific bacterial groups.
Article
Microbiology
Zhong-Zhi Sun, Bo-Wen Ji, Ning Zheng, Meng Wang, Ye Cao, Lu Wan, Yi-Song Li, Jin-Cheng Rong, Hai-Lun He, Xiu-Lan Chen, Yu-Zhong Zhang, Bin-Bin Xie
Summary: Deconstruction of polysaccharides is important in their conversion, with polysaccharide-degrading enzymes playing a key role. This study investigated the phylogenetic distribution and correlations of nine types of these enzymes in marine bacterial genomes, revealing coding potentials and conservation at the genus level. The utilization of polysaccharides may contribute to the ecological differentiation of marine bacteria, with significant correlations observed between different enzymes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Tra T. H. Nguyen, Tien Q. Vuong, Ho Le Han, Zhun Li, Yong-Jae Lee, Jaeho Ko, Olga I. Nedashkovskaya, Song-Gun Kim
Summary: In this study, three novel gliding strains, designated as SS9-22(T), W9P-11(T), and SW1-E11(T), were isolated from algae and decaying wood and proposed as three novel species of the genus Fulvivirga. Genomic analysis identified a large number of genes encoding carbohydrate-active enzymes that potentially participate in polysaccharide degradation. In vitro experiments confirmed the ability of these strains to degrade various polysaccharides, highlighting their potential applications in biotechnology.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Sylvia Colliec-Jouault, Fabiana Esposito, Helene Ledru, Corinne Sinquin, Laetitia Marchand, Arnaud Fillaudeau, Sylvain Routier, Frederic Buron, Chrystel Lopin-Bon, Stephane Cuenot, Emiliano Bedini, Agata Zykwinska
Summary: Sulfated glycosaminoglycans (GAGs) play important roles in cell-cell and cell-matrix interactions. This study explores the use of microwave-assisted sulfation to obtain highly sulfated GAG mimetics. NMR structural analysis shows that microwave-assisted sulfation is as efficient as the conventional method, with a 12-fold shorter reaction time.
Article
Environmental Sciences
Kuo-Jian Ma, Yong-Lian Ye, Yun-Han Fu, Ge-Yi Fu, Cong Sun, Xue-Wei Xu
Summary: This study investigated the microbial diversity in tidal flats and focused on the role of Bacteroidota strains in polysaccharide degradation. A total of 352 pure cultured bacteria were isolated and mainly belonged to Bacteroidota, Pseudomonadota, Bacillota, and Actinomycetota. Three Bacteroidota strains were selected for taxonomic analysis and shown to represent novel species or genera. Genomic analysis revealed their potential in polysaccharide metabolism.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hairong Huang, Jiajun Chen, Xiaobo Hu, Yi Chen, Jianhua Xie, Tianxiang Ao, Hui Wang, Jiayan Xie, Qiang Yu
Summary: This study aims to investigate the interaction effect between dietary fiber components and bound polyphenol components in tea residues (TRDF) on the anti-hyperglycemic activity. The results showed that bound polyphenol components are essential for the anti-hyperglycemic activity of TRDF, and they also modulate gut microbiota and increase the content of short chain fatty acids.
Article
Microbiology
Angela K. Boysen, Bryndan P. Durham, William Kumler, Rebecca S. Key, Katherine R. Heal, Laura T. Carlson, Ryan D. Groussman, E. Virginia Armbrust, Anitra E. Ingalls
Summary: This study investigated the uptake kinetics and metabolic fate of GBT in two microbial communities in the North Pacific. The results showed that the uptake rate and metabolism of GBT varied with nitrate concentrations. These findings reveal the complex biochemical potential of GBT and the significance of microbial metabolite exchange.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Yuanyuan Dai, Nadezda Ignatyeva, Hang Xu, Ruheen Wali, Karl Toischer, Soeren Brandenburg, Christof Lenz, Julius Pronto, Funsho E. Fakuade, Samuel Sossalla, Elisabeth M. Zeisberg, Andreas Janshoff, Ingo Kutschka, Niels Voigt, Henning Urlaub, Torsten Bloch Rasmussen, Jens Mogensen, Stephan E. Lehnart, Gerd Hasenfuss, Antje Ebert
Summary: This study identified impaired subcellular iron uptake mechanisms in cardiomyocytes of heart failure patients, which are independent of systemic iron metabolism. The defects in clathrin-mediated endocytosis and cargo transfer were found to be responsible for subcellular iron deficiency in dilated cardiomyopathy. Restoring the molecular pathway through genetic correction, peptide treatment, or iron supplementation rescued the dysfunction and improved contractility, indicating a potential treatment strategy for heart failure.
CIRCULATION RESEARCH
(2023)
Article
Environmental Sciences
Jordana Georgin, Matias S. Netto, Dison S. P. Franco, Daniel G. A. Piccilli, Katia da Boit Martinello, Luis F. O. Silva, Edson L. Foletto, Guilherme L. Dotto
Summary: The study demonstrates that activated carbon derived from grape branches is effective in removing naproxen and treating synthetic mixtures containing drugs and salts, with a removal efficiency of 95.63%. Furthermore, the adsorbent can be regenerated up to 7 times using an HCl solution, showcasing its sustainable reuse potential.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Medicinal
Han Yan, Jie Wang, Jiayi Yang, Zhongqiu Xu, Chunxia Li, Cui Hao, Shixin Wang, Wei Wang
Summary: This study explored the anti-HSV-2 activity of a marine sulfated polysaccharide known as PSSD. It was found that PSSD demonstrated significant anti-HSV-2 activity with low cytotoxicity in vitro. PSSD was observed to directly interact with virus particles to prevent virus adsorption and inhibit virus-induced membrane fusion. In vivo experiments showed that PSSD effectively attenuated genital herpes symptoms, reduced virus shedding, and outperformed acyclovir. These findings highlight the potential of PSSD as a novel anti-genital herpes agent.
Article
Thermodynamics
Eduardo Mueller-Casseres, Francielle Carvalho, Tainan Nogueira, Clarissa Fonte, Mariana Imperio, Matheus Poggio, Huang Ken Wei, Joana Portugal-Pereira, Pedro R. R. Rochedo, Alexandre Szklo, Roberto Schaeffer
Summary: The study provides an IAM perspective on the production and distribution of alternative marine fuels in Brazilian ports, in line with the IMO2050 emission reduction target. It highlights the potential drawbacks of achieving the IMO2050 goal without considering a national decarbonization strategy, and emphasizes the need for an integrated mitigation strategy to effectively decarbonize the entire marine supply chain.
Review
Multidisciplinary Sciences
Pipuni Tennakoon, Pathum Chandika, Myunggi Yi, Won-Kyo Jung
Summary: The manufacturing and consumption of plastic products have caused depletion of petroleum resources and increased environmental pollution. Biobased plastics, while introduced as an alternative, have negative impacts on the environment and livelihoods. Marine-derived bioplastics offer a promising and cost-effective solution, but further research is needed. This review summarizes the raw materials, extraction processes, and properties of marine-derived bioplastics to accelerate their production.
Article
Biochemical Research Methods
Noele Norris, Naomi M. Levine, Vicente I. Fernandez, Roman Stocker
Summary: Marine bacterial diversity is influenced by metabolic strategies, with copiotrophs and oligotrophs exhibiting trade-offs in nutrient transport systems. Oligotrophs rely on binding proteins for nutrient uptake, achieving high affinities but limiting growth rates due to large periplasms and slow diffusion of binding proteins. This trade-off has shaped the evolution of oligotrophs and copiotrophs, providing insights into their physiological differences in nutrient-rich and nutrient-poor environments.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Ecology
Nina Bartlau, Antje Wichels, Georg Krohne, Evelien M. Adriaenssens, Anneke Heins, Bernhard M. Fuchs, Rudolf Amann, Cristina Moraru
Summary: This study suggests that phages may modulate the succession of heterotrophic bacteria following phytoplankton blooms in spring. The diverse flavophages identified have the potential to affect their respective host populations in the North Sea microbial community, as stable components.
Article
Ecology
Luis H. Orellana, T. Ben Francis, Marcela Ferraro, Jan-Hendrik Hehemann, Bernhard M. Fuchs, Rudolf Amann
Summary: The study found that small, coccoid, free-living Verrucomicrobiota specialize in consuming fucose and rhamnose during spring algal blooms in the North Sea. These Verrucomicrobiota populations accounted for up to 8% of the bacterioplankton and actively expressed proteins critical for the consumption of fucosylated and sulfated polysaccharides during consecutive spring bloom events. Specialized metabolic pathways of Verrucomicrobiota could determine the fate of complex polysaccharides consumed during algae blooms, particularly those containing methyl pentose sugars.
Article
Ecology
Cecilia Alonso, Emiliano Pereira, Florencia Bertoglio, Miguel De Caceres, Rudolf Amann
Summary: Increasing awareness of environmental impacts caused by anthropogenic activities emphasizes the importance of determining environmental status indicators that can be assessed on a large scale. This study analyzed bacterioplankton communities in four estuaries worldwide and identified indicators based on physicochemical variables. These bacterial indicators showed high prediction capability and outperformed other biological assemblages commonly used for environmental monitoring. The study suggests that this approach could complement existing strategies for monitoring and conserving aquatic systems globally.
AQUATIC MICROBIAL ECOLOGY
(2022)
Article
Ecology
Bledina Dede, Christian T. Hansen, Rene Neuholz, Bernhard Schnetger, Charlotte Kleint, Sharon Walker, Wolfgang Bach, Rudolf Amann, Anke Meyerdierks
Summary: The study analyzed the microbial ecology of three different hydrothermal plumes in the Kermadec intra-oceanic arc in the South Pacific Ocean, revealing niche differentiation of the SUP05 clade based on sulfide and iron concentration as well as water depth.
Article
Ecology
Taylor Priest, Anneke Heins, Jens Harder, Rudolf Amann, Bernhard M. Fuchs
Summary: In this study, we investigate the species-level niche partitioning within the NS5 Marine Group by combining spatiotemporal dynamics and predicted substrate utilization. We identify depth as a key influencing factor in the distribution dynamics of NS5 species, and observe successional dynamics in relation to specialized substrate adaptations. Additionally, we provide a complete genome sequence and morphological insights for Arcticimaribacter forsetii AHE01FL, a candidate species within the NS5 group.
Article
Microbiology
Greta Giljan, Carol Arnosti, Inga Kirstein, Rudolf Amann, Bernhard M. Fuchs
Summary: Marine heterotrophic bacteria play a significant role in global carbon cycling by utilizing phytoplankton-derived polysaccharides. The utilization patterns and rates of polysaccharides vary seasonally, along with changes in bacterial communities. This study found that the mode and speed of polysaccharide utilization differed between seasons, as did the bacterial clades involved.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Ecology
Vipul Solanki, Karen Krueger, Conor J. Crawford, Alonso Pardo-Vargas, Jose Danglad-Flores, Kim Le Mai Hoang, Leeann Klassen, D. Wade Abbott, Peter H. Seeberger, Rudolf I. Amann, Hanno Teeling, Jan-Hendrik Hehemann
Summary: This study confirms the presence of a GH76 endo-alpha-1,6-mannanase in the marine bacterium Salegentibacter sp. Hel_I_6, which is similar to enzymes found in gut bacteria. The enzyme degrades fungal mannan oligosaccharides during microalgae blooms, and structural analysis shows adaptation to this type of substrate at the molecular level.
Editorial Material
Biotechnology & Applied Microbiology
Ramon Rossello-Mora, Konstantinos T. Konstantinidis, Rudolf Amann
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Ecology
Bledina Dede, Taylor Priest, Wolfgang Bach, Maren Walter, Rudolf Amann, Anke Meyerdierks
Summary: In this study, the microbial community composition of hydrothermal plumes in the Northern Tonga arc and Northeastern Lau Basin was analyzed using multiple culture-independent techniques, with a focus on the autecology of Alcanivorax. Alcanivorax was found to dominate the community at two sites, despite no indication for hydrocarbon presence in the plumes. The high abundance and gene expression of Alcanivorax in these areas may be related to undiscovered hydrocarbon seepage from the seafloor, potentially resulting from recent volcanic activity.
Article
Environmental Sciences
Greta Reintjes, Anneke Heins, Cheng Wang, Rudolf Amann
Summary: Particulate organic matter is crucial in the marine carbon cycle for transporting organic carbon from surface to deep oceans. By analyzing particles and their microbiomes, we found significant differences between particle-attached and free-living bacterial communities in terms of composition, dynamics, and catabolic potential. We also discovered the dominance of specific bacterial taxa and their roles in glycan degradation, indicating niche diversification in particle-attached bacteria.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Ecology
Greta Giljan, Sarah Brown, C. Chad Lloyd, Sherif Ghobrial, Rudolf Amann, Carol Arnosti
Summary: Heterotrophic bacteria in the ocean invest in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake. Selfish bacteria change the odds in their favor by transporting polysaccharides without loss of hydrolysis products. Surprisingly, selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. The activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
ISME COMMUNICATIONS
(2023)
Correction
Microbiology
Tomeu Viver, Luis H. Orellana, Janet K. Hatt, Mercedes Urdiain, Sara Diaz, Michael Richter, Josefa Anton, Massimo Avian, Rudolf Amann, Konstantinos T. Konstantinidis, Ramon Rossello-Mora
ENVIRONMENTAL MICROBIOLOGY
(2022)