Reyranella terrae sp nov., isolated from an agricultural soil, and emended description of the genus Reyranella

A novel strain designated 11G32(T) was isolated from an agricultural soil cultivated with Chinese cabbage in Korea. The cells were Gram-stain-negative, aerobic, non-motile and rod-shaped. The strain grew at 15-28 degrees C (optimum, 20 degrees C), pH 5.0-7.0 (optimum, pH 5.0-6.0) and without NaCl. Phylogenetically, the strain was found to be closely related to members of the genus Reyranella and showed 16S rRNA gene sequence similarities of 97.18, 96.76 and 95.99% with Reyranella graminifolii Wo-34 T, Reyranella massiliensis 521(T) and Reyranella soli KIS14-15(T), respectively. The major fatty acids were C-19 : 0 cyclo omega 8c, C-16 : 0, summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c) and 11-methyl C-18 : 1 omega 7c. The predominant ubiquinone was Q-10. The polar lipids profile revealed the presence of phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, unidentified aminolipids, unidentified phospholipids and unidentified lipids. On the basis of data presented, strain 11G32(T) is considered to represent a novel species of the genus Reyranella, for which the name Reyranella terrae sp. nov. is proposed. The type strain is 11G32(T) (= KACC 18486(T) = NBRC 111476(T)).