Cohnella algarum sp nov., isolated from a freshwater green alga Paulinella chromatophora

A Gram-stain-positive, facultatively aerobic and endospore-forming bacterium, designated strain Pch-40(T), was isolated from a freshwater green alga, Paulinella chromatophora. Cells were motile rods with a monotrichous polar flagellum showing catalase-and oxidase-positive reactions. Strain Pch-40(T) grew at 20-50 degrees C (optimum, 37-40 degrees C), at pH 5.0-11.0 (optimum, pH 7.0) and in the presence of 0-4.0% (w/v) NaCl (optimum, 0 %). Menaquinone-7 was detected as the sole isoprenoid quinone. The genomic DNA G+C content of strain Pch-40(T) was 55.6 mol%. The major cellular fatty acids of strain Pch-40(T) were C-16 : 0, iso-C-16 : 0, anteiso-C-15 : 0 and anteiso-C-17 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Pch-40(T) clearly belonged to the genus Cohnella of the family Paenibacillaceae. Strain Pch-40(T) was most closely related to Cohnella rhizosphaerae CSE-5610(T) with a 96.1% 16S rRNA gene sequence similarity. The phenotypic and chemotaxonomic features and the phylogenetic inference clearly suggested that strain Pch-40(T) represents a novel species of the genus Cohnella, for which the name Cohnella algarum sp. nov. is proposed. The type strain is strain Pch-40(T) (= KACC 19279(T) = JCM 32033(T)).