Proposal of Henriciella barbarensis sp nov and Henriciella algicola sp nov., stalked species of the genus and emendation of the genus Henriciella

Two Gram-negative, heterotrophic, aerobic, prosthecated, marine bacteria, designated strains MCS23(T) and MCS27(T), were isolated from seawater samples. NaCl was required for growth. The major polar lipid detected in strain MCS27(T) was phosphatidylglycerol, whereas those detected in MCS23(T) were phosphatidylglycerol, sulfoquinovosyl diacylglycerol and 1,2diacyl-3-a-D-glucuronopyranosyl-sn-glycerol taurineamide. The most abundant cellular fatty acids were C-18 : 1 omega 7 and C-16: 0, hydroxyl-fatty acids were 3-OH C-12:0 in both strains and 3-OH C-11: 0 in MCS23(T). Strains MCS23(T) and MCS27(T) had DNA G+C contents of 57.0 and 55.0 mol%, respectively. The two strains shared 99.3% 16S rRNA gene sequence similarity; levels of similarity with the type strains of species of the genus Henriciella were 99.4-97.8% but DNA-DNA hybridizations were 53% or lower. Besides their 16S rRNA gene sequences, the novel strains can be differentiated from other species of the genus Henriciella by cell morphology, lipid and fatty acid patterns and enzyme activities. The data obtained led to the identification of two novel species, for which the names Henriciella barbarensis sp. nov. (type strain MCS23(T) = LMG 28705(T) = CCUG 66934(T)) and Henriciella algicola sp. nov. (type strain MCS27(T) = LMG 29152(T) = CCUG 67844(T)) are proposed. As these two novel species are the first prosthecate species in the genus Henriciella, an emended genus description is also provided.