Luteolibacter flavescens sp nov., isolated from deep seawater

A novel Gram-stain-negative, rod-shaped, non-motile strain, designated GKXT, was isolated from deep seawater. Strain GKX(T) was able to grow at 20-35 degrees C (optimum, 25 degrees C), pH 5.5-9.5 (optimum, 7.5) and 0-4.0% (w/v) NaCl (optimum, 1.0%). The major fatty acids were C-16:1 omega 9c (15.4%), C-16:0 (18.4%), C-14:0 (12.0%), iso-C-14:0 (30.1%) and anteiso-C-15:0 (5.7%). Strain GKX(T) contained phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid as the main polar lipids. The only isoprenoid quinone was menaquinone-9. The diagnostic amino acids of the cell-wall peptidoglycan contained meso-diaminopimelic acid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GKX(T) belonged to the genus Luteolibacter in the family Verrucomicrobiaceae. The 16S rRNA gene sequence of this strain showed 98.0, 93.5 and 93.3% sequence similarity, respectively, with those of Luteolibacter arcticus MC 3726(T), L.uteolibacter pohnpeiensisA4T-83(T) and L.uteolibacter cuticulihirudinis E100(T). DNA-DNA hybridization value of strain GKX(T) with L. arcticus MC 3726(T) was 33.1?%. The G+C content of the genomic DNA was 59.5 mol%. On the basis of the genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain GKX(T) was proposed to represent a novel species of the genus Luteolibacter, named Luteolibacter flavescens sp. nov. (type strain GKX(T)=MCCC 1K03193(T)=KCTC 52361(T)).