期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 32, 期 -, 页码 123-130出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2015.03.010
关键词
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资金
- ERC [281331]
- Swiss National Science Foundation [CRSII3_136254]
- Baden-Wurttemberg Stiftung
- Swiss National Science Foundation (SNF) [CRSII3_136254] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [281331] Funding Source: European Research Council (ERC)
Most membrane-proteins exist in complexes rather than isolated entities. To fully understand their biological function it is essential to study the intact membrane-protein assemblies. The overexpression and purification of many essential membrane-protein complexes is still a considerable and often unsurmountable challenge. In these cases, extraction from source is the only option for many large multi-subunit cellular machines. Here, we describe recent advances in overexpression of multi-subunit membrane-protein complexes, the strategies to stabilize these complexes and highlight major achievements in membrane-protein structural research that were facilitated by the prospect of achieving subnanometer to near-atomic resolution by electron cryo-microscopy.
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