Article
Nutrition & Dietetics
Makoto Inoue, Yuichi Tanaka, Sakiko Matsushita, Yuri Shimozaki, Hirohito Ayame, Hidenori Akutsu
Summary: This study utilized xenogeneic-free human intestinal organoids (XF-HIOs) to investigate nutrient absorption and the inhibitory effects of natural ingredients. The results showed that epicatechin gallate and nobiletin inhibited glucose absorption, while luteolin and quercetin did not inhibit cholesterol absorption.
Article
Pharmacology & Pharmacy
Austin D. Fergusson, Rui Zhang, Judy S. Riffle, Richey M. Davis
Summary: Flash nanoprecipitation (FNP) is a reliable method for producing polymer nanoparticles loaded with hydrophobic drugs. By incorporating ion pairing agents (IPs) into the FNP formulation, hydrophobic drug salts can be efficiently precipitated. In this study, we investigated the effects of two hydrophobic IPs, palmitic acid (PA) and hexadecylphosphonic acid (HDPA), on the loading and size of LY294002-loaded nanoparticles. HDPA resulted in well-defined colloidally stable particles, while PA resulted in ill-defined aggregates. This approach opens up possibilities for intravenous administration of previously unusable hydrophobic drugs.
Article
Chemistry, Physical
Henrique Trevisan, Kana Nishimori, Stefano Aime, Jean-Michel Guigner, Makoto Ouchi, Francois Tournilhac
Summary: The selection of monomer couples with close to zero reactivity ratios, along with the customization of solvent-monomer interactions, has been shown to induce spontaneous copolymerization into nanoparticles with self-assembly properties. The ouzo effect was utilized to study the formation of stable alternating copolymer nanoparticles in water, without the need for stabilizing agents. Characterization techniques such as light scattering and transmission electron microscopy confirmed the successful self-assembly of copolymer chains into nanoparticles, with the size affected by the lipophilicity of the copolymers and solvent properties.
Article
Biochemistry & Molecular Biology
Nava Reznik, Annastassia D. Gallo, Katherine W. Rush, Gabriel Javitt, Yael Fridmann-Sirkis, Tal Ilani, Noa A. Nairner, Simon Fishilevich, David Gokhman, Kelly N. Chacon, Katherine J. Franz, Deborah Fass
Summary: This study reveals the protective role of mucus in managing toxic transition metal copper by blocking futile redox cycling while allowing copper uptake into cells. The importance of molecular structure in advancing mucosal biology is highlighted, introducing mucins as extracellular copper chaperones produced in massive quantities to guard extensive mucosal surfaces.
Article
Gastroenterology & Hepatology
Junsik Sung, Lixin Wang, Dingpei Long, Chunhua Yang, Didier Merlin
Summary: Genetic knockout of PepT1 protein provides resistance to acute colitis and colitis-associated cancer in mouse models. The altered fecal metabolites, particularly tuberonic acid (TA), in PepT1 KO mice play a crucial role in anti-inflammatory effects and epithelial cell healing processes. This study sheds light on the potential mechanisms associated with the protective effects of PepT1 KO.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Chemistry, Physical
Antoine Combes, Khanh-Nam Tang, Andrey S. Klymchenko, Andreas Reisch
Summary: The study investigates the assembly of mixed-charge polymer nanoparticles through nanoprecipitation of mixtures of oppositely charged polymers, examining the influence of charge fraction, polymer mixing ratio, and precipitation conditions on particle size and surface charge. The results show the potential of this method for designing nanoparticles with precisely tuned surface properties.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Jingyi Li, Yaqi Zhang, Miaorong Yu, Aohua Wang, Yu Qiu, Weiwei Fan, Lars Hovgaard, Mingshi Yang, Yiming Li, Rui Wang, Xiuying Li, Yong Gan
Summary: This study demonstrates that the upregulated intestinal transporter PCFT facilitates the uptake and intracellular trafficking of ligand-modified nanoparticles. In cells with low expression of PCFT, nanoparticles are predominantly degraded in lysosomes.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Polymer Science
Qimeng Zhang, Xiaopeng Pei, Kepeng Hu, Yating Zhou, Ming-Liang Ma, Mingquan Wang, Huiyong An, Ying Tan
Summary: The study presents a facile, rapid, and cost-effective strategy for preparing starch-based microrods with tunable aspect ratios via shear-assisted antisolvent-induced nanoprecipitation and solidification. The resulting microrods have potential applications as drug carriers and biologically active materials.
Review
Materials Science, Multidisciplinary
William N. Sharratt, Victoria E. Lee, Rodney D. Priestley, Joao T. Cabral
Summary: This review compares two versatile approaches for the precise formation of polymer particles: flash nanoprecipitation (FNP) and droplet solvent extraction (DSE). FNP generates nanoparticles with diameters ranging from 10-1000 nm, while DSE produces particles with diameters from 1-1000 µm. Both methods involve a series of processes leading to a variety of internal and external particle morphologies.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Chemistry, Medicinal
Qikun Jiang, Qiuchi Xu, Yingli Wang, Pengyan Li, Yunran Zhang, Yongjun Wang, Jin Sun, Tianhong Zhang, Zhonggui He
Summary: By modulating the chirality of the linear dipeptide HS-tLL, the colon-targeting efficiency can be enhanced, providing a potential treatment for inflammatory bowel disease (IBD).
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Engineering, Chemical
Nicole Aegerter, Alex Luijten, Daniele Massella, Paolo Ermanni
Summary: This research proposes a modified additive manufacturing route to efficiently manufacture polypropylene micromixers and produce stable nanosuspensions through flash nanoprecipitation (FNP) process.
Review
Pharmacology & Pharmacy
Carlynne Choy, Lee Yong Lim, Lai Wah Chan, Zhixiang Cui, Shirui Mao, Tin Wui Wong
Summary: This review examines the potential of targeting intestinal receptors to improve the oral delivery of nanoinsulin. While promising, further research and optimization are needed.
PHARMACOLOGICAL REVIEWS
(2022)
Article
Engineering, Chemical
Wei Li, Qiaoli Chen, Thejus Baby, Song Jin, Yun Liu, Guangze Yang, Chun-Xia Zhao
Summary: This study investigated the fundamental principles of polymer nanoparticle drug encapsulation through microfluidic technology. Using curcumin and PEG-PLGA as a model system, the research revealed the different precipitation and mixing times of the two components in the microfluidic device, leading to low drug loading. This provides a new perspective on understanding and controlling the formation of drug-loaded polymeric nanoparticles and offers guidelines for selecting the appropriate combinations of drugs, polymers, solvents, and devices.
CHEMICAL ENGINEERING SCIENCE
(2021)
Article
Chemistry, Physical
Moritz Beck-Broichsitter
Summary: Polymer nanoparticles can be prepared by nanoprecipitation, with the size depending on the choice of organic solvent, polymer concentration, and exchange ratios. The quality of the organic solvent plays a crucial role in determining the size properties of the obtained polymer nanoparticles, emphasizing the importance of systematic solvent selection in nanomedicine.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Review
Food Science & Technology
Zixin Guo, Yixiang Liu, Yangchao Luo
Summary: This paper explores the role of dietary lipids in the absorption and utilization of carotenoids, discussing the mechanisms of intestinal absorption, regulation of lipid transporters and intracellular metabolizing enzymes, and the efflux process of chyle particles. It emphasizes the importance of selecting appropriate dietary lipids for the absorption and utilization of different carotenoids.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Pharmacology & Pharmacy
Camille Cotteret, Philippe-Henri Secretan, Laurence Gilles-Afchain, Julia Rousseau, Fabrice Vidal, Gabriel Salguero-Hernandez, Jonathan Batista, Vicky Valverde, Jerome Guitton, Salvatore Cisternino, Joel Schlatter
Summary: This study aims to investigate drug contamination of the exterior surfaces of cytotoxic drug vials. The results showed significant trace amounts of cytotoxic drugs on both unprotected and protected plastic shrink wrap vials, indicating that the current secondary packaging does not effectively prevent exposure to handlers. Therefore, it is necessary to establish guidelines and safety recommendations.
EUROPEAN JOURNAL OF HOSPITAL PHARMACY
(2022)
Article
Toxicology
Guillaume Bianconi, Isabelle Malissin, Laurence Labat, Nihel Khoudour, Pascal Houze, Nicolas Pallet, Bruno Megarbane, Xavier Decleves
Summary: Tramadol poisoning in a kidney transplant patient resulted in marked and prolonged serotonin toxicity, attributed to increased production of M2 due to inhibition of tramadol metabolism by paroxetine and propranolol. This patient had normal CYP2D6-metabolizer status based on genotyping, but the metabolic profile revealed the impact of strong mechanism-based inhibitors on tramadol metabolism, leading to prolonged serotonin toxicity caused by elevated M2 levels.
CLINICAL TOXICOLOGY
(2022)
Letter
Infectious Diseases
Romain Gueneau, Brune Joannard, Naeda Haddad, Fanny Alby, Vincent Jullien, Joel Schlatter, Camille Cotteret, Marie Elisabeth Bougnoux, Fanny Lanternier, Liliane Laroche, Sarah Delliere, Salvatore Cisternino, Olivier Lortholary
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Biochemistry & Molecular Biology
Celine Chevaleyre, Dimitri Kereselidze, Fabien Caille, Nicolas Tournier, Nagore G. Olaciregui, Alexandra Winkeler, Xavier Decleves, Benoit Jego, Salvatore Cisternino, Sylvain Auvity, Charles Truillet
Summary: For Diffuse intrinsic pontine gliomas (DIPG), molecular imaging may be an ideal technique for targeting and monitoring tumor evolution. MR remains the imaging technique of reference but often fails to define tumor extent, which is crucial for therapeutic efficacy assessment. PET offers a unique way to target specific biomarkers in vivo and may be a promising biomarker for monitoring DIPG tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Anatomy & Morphology
Leila Slaoui, Alice Gilbert, Armelle Rancillac, Barbara Delaunay-Piednoir, Audrey Chagnot, Quentin Gerard, Gaelle Letort, Philippe Mailly, Noemie Robil, Antoinette Gelot, Mathilde Lefebvre, Maryline Favier, Karine Dias, Laurent Jourdren, Laetitia Federici, Sylvain Auvity, Salvatore Cisternino, Denis Vivien, Martine Cohen-Salmon, Anne-Cecile Boulay
Summary: Through transcriptomic analysis, this study investigates the molecular and functional development of endothelial cells, vascular smooth muscle cells, and fibroblasts during the postnatal phase. The acquisition of contractility in vascular smooth muscle cells is crucial for increased cerebral blood flow.
BRAIN STRUCTURE & FUNCTION
(2023)
Article
Immunology
Salam Abbara, Alexandra F. Freeman, Jeremie F. Cohen, Stephanie Leclerc-Mercier, Lauren Sanchez, Joel Schlatter, Salvatore Cisternino, Ruth Parker, Edward W. Cowen, Claire Rouzaud, Marie Elisabeth Bougnoux, Fanny Lanternier, Michail S. Lionakis, Olivier Lortholary
Summary: In this study, we describe four patients with STAT3-dominant negative mutations who developed cutaneous fusariosis. This rare syndrome is characterized by chronic skin lesions that require multimodal therapies for treatment.
JOURNAL OF CLINICAL IMMUNOLOGY
(2022)
Article
Clinical Neurology
Ai Tien Nguyen, Camille Cotteret, Chloe Durrleman, Christine Barnerias, Marie Hully, Cyril Gitiaux, Bettina Mesples, Jacinta Bustamante, Stephanie Chhun, Claire Fayard, Salvatore Cisternino, Jean-Marc Treluyer, Isabelle Desguerre, Melodie Aubart
Summary: This study aimed to assess the indications and safety of rituximab (RTX) for children and identified risk factors for early B-cell repopulation. The study confirmed the good tolerance of RTX in the treatment of specific neurological disorders in a pediatric population. It also highlighted risk factors for early B-cell repopulation and emphasized the importance of B-cell monitoring.
PEDIATRIC NEUROLOGY
(2022)
Article
Public, Environmental & Occupational Health
Scarlett Wise, Fanny Lanternier, Camille Cotteret, Celine Chasport, Virginie Juin-Leonard, Amelie Cantat, Anne Scemla, Claire Delage, Barbara Mantz, Caroline Telion, Pierre Carli, Pierre Frange, Salvatore Cisternino
Summary: This study describes the organizational process and adaptability of a Covid-19 vaccination center in a French University Hospital. From January to June, the multidisciplinary team dealt with various challenges and unexpected situations by adjusting their processes. Issues encountered included supply shortages, vaccine manipulation, targeted populations, pharmacovigilance, and general communication.
HEALTH SCIENCE REPORTS
(2023)
Article
Chemistry, Analytical
David Balakirouchenane, Axelle Vasseur, Fideline Bonnet-Serrano, Minna Choi, Nihel Khoudour, Alicja Puszkiel, Lionel Groussin, Michel Vidal, Xavier Decleves, Jerome Bertherat, Benoit Blanchet
Summary: A LC-MS/MS method was developed and validated for the quantification of ODT and MTP in human plasma. The method was accurate and precise, and can be used for plasma drug monitoring during the dose-titration period.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Pharmacology & Pharmacy
Lea Darnaud, Clement Delage, Youssef Daali, Anne-Priscille Trouvin, Serge Perrot, Nihel Khoudour, Nadia Merise, Laurence Labat, Bruno Etain, Frank Bellivier, Celia Lloret-Linares, Vanessa Bloch, Emmanuel Curis, Xavier Decleves
Summary: Drug-metabolizing enzymes and drug transporters play crucial roles in drug pharmacokinetics and response. The cocktail-based phenotyping approach involves administering multiple probe drugs to evaluate the activities of CYP and transporter simultaneously. This study determined the range of phenotyping indices in healthy volunteers and classified patients with therapeutic issues based on CYP and P-gp activities.
Article
Pharmacology & Pharmacy
Benjamin Querin, Arnaud Schweitzer-Chaput, Salvatore Cisternino, Sylvain Auvity, Anne-Sophie Fauqueur, Abdel Negbane, Alice Hadchouel, Joel Schlatter, Camille Cotteret
Summary: This study developed an optimized pediatric pharmaceutical formulation of L-Methionine (Met) powder that can be reconstituted with water to obtain a stable oral suspension for children's treatment. The organoleptic characteristics and physicochemical stability of the powder and suspension were evaluated at different storage temperatures, showing no drug loss, pH changes, microbiological growth, or visual changes for an extended period of time. The developed formulation facilitates the preparation, administration, dose adjustment, and palatability of Met treatment in children.
Article
Infectious Diseases
Oussama Mouri, Clea Melenotte, Romain Guery, Camille Cotteret, Arnaud Schweitzer-Chaput, Alice Perignon, Marc Thellier, Emmanuelle Bourrat, Florentia Kaguelidou, Jean Yves Siriez, Denis Malvy, Jean-Pierre Gangneux, Alexandre Duvignaud, Christophe Ravel, Salvatore Cisternino, Janet Ransom, Eric Caumes, Olivier Lortholary, Max Grogl, Pierre Buffet
Summary: A study found that the use of a topical cream containing antibiotics was safe and effective in treating cutaneous leishmaniasis. The self-application of the cream also showed good results.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Pharmacology & Pharmacy
Blaise Pasquiers, Salih Benamara, Mathieu Felices, David Ternant, Xavier Decleves, Alicja Puszkiel
Summary: The objective of this study was to develop a generic physiologically based pharmacokinetic (PBPK) translational approach for monoclonal antibodies (mAb) using open-source software. The translation of bevacizumab was used as a case example to demonstrate the model. The predictions of the model were within an acceptable range for both area under the concentration-time curve (AUC) and maximum concentration (C-max).
Article
Cell Biology
Morgane Boulch, Marine Cazaux, Alexis Cuffel, Mathilde Ruggiu, Vincent Allain, Beatrice Corre, Yann Loe-Mie, Benoit Hosten, Salvatore Cisternino, Sylvain Auvity, Catherine Thieblemont, Sophie Caillat-Zucman, Philippe Bousso
Summary: CD4+ CAR T cells are the main drivers of cytokine release syndrome (CRS). Adjusting the CD4:CD8 CAR T cell ratio may help mitigate CAR T cell-associated toxicities.
CELL REPORTS MEDICINE
(2023)
Article
Oncology
Thibaud Derippe, Sylvain Fouliard, Ibtissam Marchiq, Sandra Dupouy, Maria Almena-Carrasco, Julia Geronimi, Xavier Decleves, Marylore Chenel, Donald E. Mager
Summary: A mechanistic pharmacokinetic/pharmacodynamic model has been developed to quantitatively support the beneficial impact of lymphodepletion before the infusion of allogeneic CAR-T cell products. Mediation through IL-7 increase and host T lymphocytes decrease is highlighted, and the model can be further used to optimize the lymphodepletion regimen in CAR-T cell therapies.
CANCER RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)