期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 12, 期 -, 页码 3171-3179出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S132776
关键词
electrochemical biosensors; Alzheimer's disease; beta-amyloid oligomer; peptide; silver nanoparticles
资金
- National Natural Science Foundation of China [61661014, 61301038]
- Natural Science Foundation of Guangxi Province [2015GXNSFBA139041]
Soluble beta-amyloid (A beta) oligomer is believed to be the most important toxic species in the brain of Alzheimer's disease (AD) patients. Thus, it is critical to develop a simple method for the selective detection of A beta oligomer with low cost and high sensitivity. In this paper, we report an electrochemical method for the detection of A beta oligomer with a peptide as the bioreceptor and silver nanoparticle (AgNP) aggregates as the redox reporters. This strategy is based on the conversion of AgNP-based colorimetric assay into electrochemical analysis. Specifically, the peptide immobilized on the electrode surface and presented in solution triggered together the in situ formation of AgNP aggregates, which produced a well-defined electrochemical signal. However, the specific binding of A beta oligomer to the immobilized peptide prevented the in situ assembly of AgNPs. As a result, a poor electrochemical signal was observed. The detection limit of the method was found to be 6 pM. Furthermore, the amenability of this method for the analysis of A beta oligomer in serum and artificial cerebrospinal fluid (aCSF) samples was demonstrated.
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