Article
Biochemistry & Molecular Biology
Yuan Gao, Xiaochen Gong, Shuang Yu, Zheng Jin, Qicheng Ruan, Chunjing Zhang, Kai Zhao
Summary: The study found that N-2-Hydroxypropyl trimethyl ammonium chloride chitosan/N, O-carboxymethyl chitosan nanoparticles (N-2-HACC/CMCS NPs) showed good immunogenicity and no toxicity as a universal vaccine adjuvant/delivery system. The prepared N-2-HACC/OVA/CMCS NPs induced higher levels of antibodies and immune responses in mice, suggesting their potential in enhancing humoral, cellular, and mucosal immune responses.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Guanyu Chen, Darren Svirskis, Weiyue Lu, Man Ying, Hongyu Li, Min Liu, Jingyuan Wen
Summary: In this study, N-trimethyl chitosan (TMC) was used as a mucoadhesive adjuvant to enhance the oral bioavailability and antitumour effects of gemcitabine formulated into nanocomplexes. The results showed that the TMC-modified nanocomplexes significantly improved the oral drug bioavailability and exhibited potent anticancer efficacy.
CARBOHYDRATE POLYMERS
(2021)
Article
Immunology
Yang Xu, Ziyou Zhou, Brad Brooks, Tammy Ferguson, Judy Obliosca, Jing Huang, Izumi Kaneko, Shiroh Iwanaga, Masao Yuda, Yukiko Tsuji, Huitang Zhang, Christina C. Luo, Xunqing Jiang, Xiang-Peng Kong, Moriya Tsuji, Christopher K. Tison
Summary: Developing a safe and effective malaria vaccine is critical in reducing the spread and resurgence of the disease, especially in children. Vaccine technology has seen advancements in the development of subunit protein, peptide, and nucleic acid vaccines, which offer advantages such as safety, immune response adaptability, simple storage requirements, easier production, and lower cost. However, these new vaccine technologies often have low efficacy. A challenge in malaria vaccine development is the complex life cycle and multiple stages of the Plasmodium organism. Targeting multistage antigens simultaneously is crucial for an effective vaccine. Scientists have designed a layer-by-layer antigen delivery platform specifically engineered for malaria vaccines, which has shown promising results in inducing immune responses and providing protection in animal studies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mathurin Seesen, Tuksin Jearanaiwitayakul, Jitra Limthongkul, Panya Sunintaboon, Sukathida Ubol
Summary: Dengue is a significant global public health concern, and the efficacy of the dengue vaccine is still debated. This study successfully stimulated a serotype-specific humoral and cellular immune response against DENV-2 by encapsidating EDIII in TMC NPs nanoparticles.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Medicine, Research & Experimental
Shima Bastaki, Surendar Aravindhan, Nasrin Ahmadpour Saheb, Mahsa Afsari Kashani, Aleksei Evgenievich Dorofeev, Fariba Karoon Kiani, Hediyeh Jahandideh, Farzaneh Beigi Dargani, Mohsen Aksoun, Afshin Nikkhoo, Ali Masjedi, Ata Mahmoodpoor, Majid Ahmadi, Sanam Dolati, Simin Namvar Aghdash, Farhad Jadidi-Niaragh
Summary: The immunotherapy method using potential tumor microenvironment modulators showed promising therapeutic responses in cancer treatment. The delivery system designed to deliver small interfering RNAs to silence the targeted genes resulted in significant downregulation of PD-L1 and STAT3 genes, as well as notable effects on tumor cell proliferation, migration, and angiogenesis, and tumor growth in vivo.
Article
Instruments & Instrumentation
Qingye Meng, Ying Sun, Hailin Cong, Hao Hu, Fu-Jian Xu
Summary: Infectious diseases like COVID-19, SARS, Ebola, and AIDS pose a global health threat, leading researchers to develop new antiviral and antibacterial drugs. Natural material chitosan, when used in collaboration with traditional medicine, has shown to enhance anti-infection capabilities.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Article
Immunology
Kai Zhao, Yuan Gao, Gaowei Hu, Lei Wang, Shangjin Cui, Zheng Jin
Summary: The study developed a VP2 protein subunit vaccine against Porcine parvovirus (PPV) using N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) as the adjuvant, showing that the vaccine induced higher antibody levels and long-term immune protection in immunized sows. However, the vaccine mainly stimulated humoral immunity rather than cellular immunity, and provided 100% protective efficacy against PPV infection in sows. This suggests that N-2-HACC could be a potential adjuvant for controlling PPV.
Article
Biochemistry & Molecular Biology
Xiaoyi Gao, Nan Liu, Zengming Wang, Jing Gao, Hui Zhang, Meng Li, Yimeng Du, Xiang Gao, Aiping Zheng
Summary: This study modified chitosan nanoparticles with mannose and applied them to intranasal vaccine delivery. The results showed that mannose-modified chitosan nanoparticles enhanced antigen absorption and improved the immune response in mice. This research provides a promising method for further study of chitosan nanoparticle modification.
Article
Medicine, Research & Experimental
Mehrnoush Safarzadeh, Samira Mohammadi-Yeganeh, Fatemeh Ghorbani-Bidkorbeh, Mostafa Haji Molla Hoseini
Summary: This study designed chitosan nanoparticles containing miR-155-expressing plasmid and investigated their potential as an adjuvant to enhance immune responses. The prepared nanoparticles showed appropriate size and potential, and effectively protected the plasmid from degradation. The nanoparticles were able to downregulate the expression of miR-155 target genes and increase nitric oxide production, resulting in a shift of T cell responses towards Th1. These findings suggest that chitosan nanoparticles containing miR-155-expressing plasmid have the potential to significantly enhance immune responses.
Article
Immunology
Hu Li, Xiao Liang, Wenze Sun, Baocan Zhuang, Yuanyuan Cao, Junling Zhang, Jilong Shen, Yongzhong Wang, Li Yu
Summary: This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18) using a novel vector called AHACNP-HG. The vaccine showed enhanced immune response, including a mixed Th1/Th2 cellular immune response and a stronger humoral immune response, in mice. It also prolonged the survival time of the mice after T. gondii infection.
MICROBIAL PATHOGENESIS
(2023)
Article
Medicine, General & Internal
Zhiguo Liu, Miao Wang, Yaxin Tian, Zhongqiu Li, Liping Gao, Zhenjun Li
Summary: Brucellosis is a severe public health problem in China. This study conducted a systematic analysis of related infection events from 2006 to 2019 and suggested strengthening preventive awareness, biosafety management, and vaccination skills.
ANNALS OF MEDICINE
(2022)
Review
Veterinary Sciences
Atieh Darbandi, Shabnam Zeighamy Alamdary, Maryam Koupaei, Roya Ghanavati, Mohsen Heidary, Malihe Talebi
Summary: This study aimed to evaluate the effects of Brucella vaccines on mouse models and discuss the potential mechanisms for the design of human vaccines. The results showed that Brucella vaccines can reduce the bacterial load and colonization rate in the spleen, increase inflammatory markers, especially IFN-gamma and IL-4, and induce the highest levels of antibodies compared to animals challenged with different virulent strains of Brucella. Additionally, mice showed the highest level of Brucella vaccine protection and clearance of Brucella strains when the immunization was delivered via the IP or IP-IN routes.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Chuan-Yu He, Yu-Zhuo Zhang, Meng-Zhi Liu, Hai-Long Zhao, Li-Song Ren, Bao-Shan Liu, Sun He, Ze-Liang Chen
Summary: In this study, it was demonstrated that combined immunization with low dose live Brucella vaccine and inactivated vaccine can reduce the dosage of live vaccine while providing a similar protective effect as high dose live vaccine.
BMC VETERINARY RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Melike Sessevmez, Genada Sinani, Alper Okyar, H. Oya Alpar, Erdal Cevher
Summary: Polymer nanoparticles prepared by polyelectrolyte complexation were reported for the first time as nanovaccines for nasal immunisation of protein antigens. These nanoparticles showed good physical stability, low toxicity, and long-term humoral and cellular immune responses in mice. Nasal administration stimulated mucosal immunity, indicating promising applications.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aldo Y. Tenorio-Barajas, Maria de la L. Olvera, Gabriel Romero-Paredes, Victor Altuzar, Efrain Garrido-Guerrero, Claudia Mendoza-Barrera
Summary: This work proposes a modified method using microfluidics combined with the microemulsion technique to manufacture chitosan nanoparticles. The synthesized nanoparticles showed improved size control and distribution compared to batch synthesis. The total encapsulation of IgG protein was confirmed during the fabrication of chitosan/IgG-loaded nanoparticles. The proposed materials have the potential to be used as carrier-delivery systems.
Article
Biochemistry & Molecular Biology
Hossein Aghamollaei, Mostafa Ghanei, Mohammad Javad Rasaee, Ali Mohammad Latifi, Hamid Bakherad, Mahdi Fasihi-Ramandi, Ramezan Ali Taheri, Seyed Latif Mousavi Gargari
Summary: A novel recombinant nanobody named V80 was isolated and produced using phage display technique, showing high specificity and affinity to cancer cells, making it a potential new tool for cancer detection and therapy.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sajjad Eshtiaghi, Razieh Nazari, Mahdi Fasihi-Ramandi
Summary: In recent years, drug-resistant infectious diseases have increased, leading to research on antimicrobial peptides as potential antibacterial agents. The modified mCM11 peptide showed potent antibacterial activity against various bacterial strains and no significant cytotoxic or apoptotic effects on eukaryotic cells.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2021)
Article
Chemistry, Physical
Media Hafezi, Mojtaba Rostami, Asieh Hosseini, Mehdi Rahimi-Nasrabadi, Mahdi Fasihi-Ramandi, Alireza Badiei, Farhad Ahmadi
Summary: In this study, a pH/ultrasound-responsive nano-carrier was prepared based on a magnetic core-shell structure and graphene quantum dots doped with nitrogen interlayer shell for controlled release of curcumin. The properties of the nano-carrier were extensively investigated and cytotoxicity assays were conducted on cells, showing no cytotoxic effects at high concentrations. The loaded curcumin exhibited high loading efficiency and sustained release, controllable by ultrasound irradiation and pH adjustment, making it suitable for targeted drug delivery.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Hamidreza Majidiani, Mahdi Fasihi-Ramandi
Summary: The study investigated the antibacterial and cytotoxic properties of d-leucine modified CM11 peptide, which showed good bactericidal effects on multidrug-resistant clinical isolates and standard strains, with a potential non-toxicity against Vero cells.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2021)
Article
Biology
Mahsa Shahbandeh, Anoosh Eghdami, Mehrdad Moosazadeh Moghaddam, Mohammadreza Jalali Nadoushan, Ali Salimi, Mahdi Fasihi-Ramandi, Saber Mohammadi, Mehrnaz Mirzaei, Reza Mirnejad
Summary: The study demonstrated that IMP-AgNPs exhibited strong antibacterial activity against multidrug-resistant Pseudomonas aeruginosa, with significant effects on resistant strains. IMP-AgNPs displayed low cytotoxicity, induced apoptosis, and positively impacted wound healing in burn injuries.
JOURNAL OF BIOSCIENCES
(2021)
Article
Engineering, Electrical & Electronic
Mojtaba Rostami, Parastoo Sharafi, Shahla Mozaffari, Kourosh Adib, Ali Sobhani-Nasab, Mehdi Rahimi-Nasrabadi, Mahdi Fasihi-Ramandi, Mohammad Reza Ganjali, Alireza Badiei
Summary: By combining different nanosheets, ZnFe2O4-CuO-C3N4 and ZnFe2O4-CuO-N/B/RGO ternary nanoheterojunctions were fabricated to reduce recombination of electron/hole pairs and enhance photocatalytic efficiency for MO degradation.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2021)
Article
Pharmacology & Pharmacy
Abdolkarim Hosseini, Hadi Esmaeili Gouvarchin Ghaleh, Hossein Aghamollaei, Mahdi Fasihi Ramandi, Gholamhossein Alishiri, Alireza Shahriary, Kazem Hassanpour, Mahdi Tat, Gholamreza Farnoosh
Summary: Studies have shown that melatonin can be used as a medicinal adjuvant to treat COVID-19 by reducing and controlling inflammatory cytokines through regulating the expression of Th1 and Th2 regulatory genes.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Immunology
Samira Dodangeh, Mahdi Fasihi-Ramandi, Ahmad Daryani, Reza Valadan, Hossein Asgarian-Omran, Zahra Hosseininejad, Tooran Nayeri Chegeni, Abdol Sattar Pagheh, Javad Javidnia, Shahabeddin Sarvi
Summary: The protein vaccine candidate MRS, based on the prediction of specific epitopes from three Toxoplasma gondii antigens, showed potential for effective protection against toxoplasmosis in mice. Co-delivery of the vaccine with CaPNs may play a key role in controlling T. gondii infection.
MICROBIAL PATHOGENESIS
(2021)
Article
Medicine, Research & Experimental
Gholamreza Farnoosh, Mostafa Akbariqomi, Taleb Badri, Mahdi Bagheri, Morteza Izadi, Ali Saeedi-Boroujeni, Ehsan Rezaie, Hadi Esmaeili Gouvarchin Ghaleh, Hossein Aghamollaei, Mahdi Fasihi-Ramandi, Kazem Hassanpour, GholamHossein Alishiri
Summary: This study evaluated the clinical efficacy of melatonin as an adjuvant treatment for COVID-19 patients. The results showed that the use of melatonin significantly improved clinical symptoms, shortened hospital stay, and had no deaths or adverse events.
ARCHIVES OF MEDICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Hadi Esmaeili Gouvarchin Ghaleh, Abdolkarim Hosseini, Hossein Aghamollaei, Mahdi Fasihi-Ramandi, Gholamhossein Alishiri, Ali Saeedi-Boroujeni, Kazem Hassanpour, Mohammad-Reza Mahmoudian-Sani, Gholamreza Farnoosh
Summary: Melatonin, as a complementary treatment for COVID-19 patients, can reduce inflammation by targeting the inflammasome multiprotein complex and oxidative stress factors, while also boosting antioxidant levels and showing beneficial effects on the immune system.
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ramezan Ali Taheri, Ali Bahramifar, Mahmoud Reza Jaafari, Mahdi Fasihi-Ramandi, Reza Zolfaghari Emameh, Saeed Mohammadian Haftcheshmeh, Maryam Ebrahimi Nik
Summary: The study aimed to evaluate the effects of four different liposomal formulations on MDSCs and Tregs in C26 tumor-bearing mice. Results showed that liposomes containing 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) significantly reduced MDSC population and activity in the spleen. This suggests that DOTAP-containing liposomes may contribute to stimulating immune responses and inhibiting immunosuppression in the tumor microenvironment.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Mohammad Moradi, Reza Golmohammadi, Ali Najafi, Mehrdad Moosazadeh Moghaddam, Mahdi Fasihi-Ramandi, Reza Mirnejad
Summary: The study aimed to suppress the activity of a critical enzyme in SARS-CoV-2 using plant-derived protease inhibitor peptides, with VcTI identified as a potentially effective inhibitory ligand. Molecular dynamic assay confirmed the stability of the selected receptor and ligand complex, further in vitro and in vivo investigations are needed to verify the therapeutic efficiency of this compound against SARS-CoV-2 infection.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2022)
Article
Biochemical Research Methods
Zohre Sadeghi, Mahdi Fasihi-Ramandi, Saeid Bouzari
Summary: The study found that BhuA, 7 alpha-HSDH or FliC antigens are effective in inducing immune responses against brucellosis, especially when used in adjuvant. Immunization with proteins in adjuvant showed higher levels of protection compared to proteins only, and induced a Th1-oriented immune response.
JOURNAL OF IMMUNOLOGICAL METHODS
(2022)
Article
Polymer Science
Maryam Saleh Mohammadnia, Salar Hemmati, Mahdi Fasihi-Ramandi, Mahmoud Bahari
Summary: A novel hybrid synthetic material combining chitosan-functionalized graphene oxide with nanohydroxyapatite particles was synthesized for dental and biomedical purposes. The resulting nanocomposite demonstrated enhanced thermal stability and mechanical properties, showing promising potential for biomedical applications.
Review
Biochemistry & Molecular Biology
Amir Tajbakhsh, Seyed Mohammad Gheibihayat, Ramezan Ali Taheri, Mahdi Fasihi-Ramandi, Abolfazl Nesaei Bajestani, Abolfazl Taheri
Summary: Efferocytosis is a non-inflammatory process that removes apoptotic cells to maintain tissue homeostasis, prevent unwanted inflammatory responses, and inhibit autoimmune responses. Soluble factors related to efferocytosis have gained attention as non-invasive biomarkers for prognosis and targeted therapy.
MOLECULAR BIOLOGY REPORTS
(2022)