Article
Oncology
Mathilde Regimbeau, Jimena Abrey, Valentin Vautrot, Sebastien Causse, Jessica Gobbo, Carmen Garrido
Summary: Heat shock proteins (HSPs) are a superfamily of molecular chaperones that play important roles in protein homeostasis, cell proliferation, differentiation, and inhibiting cell death. In cancer cells, HSPs are highly abundant and have been found to be involved in processes such as apoptosis regulation, immune responses, angiogenesis, metastasis, and treatment resistance. Additionally, cancer cells can secrete HSPs through exosomes, which can serve as biomarkers for cancer dissemination, therapy response, and patient outcome. These extracellular HSPs also have emerging functions in modulating anticancer immune responses. This review focuses on describing the recently reported functions of HSP-exosomes as therapeutic targets and disease biomarkers in cancer, as well as their potential in cancer vaccines.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biochemical Research Methods
Alvaro Ras-Carmona, Marta Gomez-Perosanz, Pedro A. Reche
Summary: The study assembled a dataset of proteins secreted by exosomes and trained random forests models to predict protein secretion by exosomes. The best model based on dipeptide composition performed well in tenfold cross-validation, and a web-based tool called ExoPred was developed for this purpose.
BMC BIOINFORMATICS
(2021)
Article
Nanoscience & Nanotechnology
Sijun Pan, Yan Zhang, Auginia Natalia, Carine Z. J. Lim, Nicholas R. Y. Ho, Balram Chowbay, Tze Ping Loh, John K. C. Tam, Huilin Shao
Summary: The platform developed in this study leverages circulating extracellular vesicles for activity-based assessment of tumour-specific drug-target interactions and can be used for clinical cancer treatment monitoring.
NATURE NANOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
R. Bonavita, G. Scerra, R. Di Martino, S. Nuzzo, E. Polishchuk, M. Di Gennaro, S. Williams, M. G. Caporaso, C. Caiazza, R. Polishchuk, M. D'Agostino, A. Fleming, M. Renna
Summary: Conformational diseases, such as Alzheimer's, Parkinson's, and Huntington's diseases, involve the aggregation and accumulation of proteins with abnormal conformations. Recent evidence suggests that the pathogenesis of these diseases may involve the transcellular transfer of mutated proteins. A novel role for the HSPB1-p62/SQSTM1 complex has been discovered, showing that it allows the unconventional secretion of mutant HTT through extracellular vesicles. This finding provides a new understanding of the mechanisms underlying Huntington's disease and may have implications for the treatment of other aggregation-prone proteins.
HUMAN MOLECULAR GENETICS
(2023)
Article
Plant Sciences
Tung-Yi Lin, Wei-Jyun Hua, Hsin Yeh, Ai-Jung Tseng
Summary: The study demonstrates that FIPs LZ-8 and GMI induce changes in the proteomic profile of tumor lesions in LLC1 cell-bearing mouse, potentially regulating heat shock proteins (HSPs)-related cell viability and inhibiting cell migration and inducing apoptosis. Inhibition of HSPs may contribute to the anti-lung cancer activity of FIPs.
Article
Biochemistry & Molecular Biology
Kohji Yamada, Kiyotsugu Yoshida
Summary: Unconventional protein secretion (UPS) is a crucial mechanism controlling the localization of cytosolic proteins lacking signal peptides and is implicated in inflammation, neurodegenerative diseases, and cancer. This article provides an overview of UPS mechanisms and categorizes cancer-specific UPS as CUPS due to their distinct features in cancer cells.
DNA AND CELL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Dong-Yi Li, Shan Liang, Jun-Hao Wen, Ji-Xin Tang, Shou-Long Deng, Yi-Xun Liu
Summary: Heat shock proteins (HSPs) are stress proteins that act as molecular chaperones and can be released into the extracellular matrix. They play important roles in inflammation and immune responses, and are involved in various human diseases, including cancers, neurodegenerative diseases, and kidney diseases. This review discusses the types of extracellular HSPs, their secretion mechanisms, and their functions in modulating inflammation and immune responses.
Review
Cell Biology
Joshua T. Farley, Mahmoud K. Eldahshoury, Carine de Marcos Lousa
Summary: Plant extracellular vesicles (EVs) play important roles in plant physiology and immunity, and plant extracts containing EVs have shown benefits for human health. Therefore, exploring the applications of plant EVs in biomedicine and their potential as drug delivery tools is of great significance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Elena Obrador, Rosario Salvador-Palmer, Ali Jihad-Jebbar, Rafael Lopez-Blanch, Thanh H. Dellinger, Ryan W. Dellinger, Jose M. Estrela
Summary: Pterostilbene has shown effectiveness against melanoma by inhibiting adrenocorticotropic hormone production in the brain and promoting cancer cell death via lysosomal membrane permeabilization. It has been found to be safe when administered intravenously in mice, suggesting its potential as an adjuvant therapy in cancer treatment. Further research is needed to optimize its bioavailability and efficacy.
Review
Immunology
Zarema Albakova, Yana Mangasarova
Summary: Heat shock proteins act as molecular chaperones, supporting tumor development by regulating various cellular processes in cancer. Investigating their intracellular and extracellular immunologic functions provides crucial insights for the development of effective immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Tzu-Yi Chen, Edgar Gonzalez-Kozlova, Taliah Soleymani, Sabrina La Salvia, Natasha Kyprianou, Susmita Sahoo, Ashutosh K. Tewari, Carlos Cordon-Cardo, Gustavo Stolovitzky, Navneet Dogra
Summary: Circulating extracellular vesicles (EVs) contain distinct proteo-transcriptomic landscape that is different from their cell of origin. These EVs enrich specific proteins and RNA associated with various cellular functions and signaling pathways. The findings suggest that EV-RNA-protein complexes may form a functional interaction network.
Article
Cell & Tissue Engineering
Ju-Fang Liu, Po-Chun Chen, Thai-Yen Ling, Chun-Han Hou
Summary: This study demonstrates that heat shock induces the expression of HSPs in hPDMCs through the activation of ROS, p38 MAPK, Akt signaling, and HSF1, which plays a protective role.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Sanjay Kumar, Brennetta J. Crenshaw, Sparkle D. Williams, Courtnee R. Bell, Qiana L. Matthews, Brian Sims
Summary: This study investigated the effects of cocaine on exosome biogenesis and composition in BV2 microglial cells. The results showed a significant decrease in exosome particles/mL after cocaine treatment, suggesting alterations in exosome formation and composition. Protein analysis also revealed changes in specific exosomal proteins, such as Hsps and Rab GTPases, indicating that cocaine modulates the expression of these proteins in microglial-derived exosomes.
NEUROCHEMICAL RESEARCH
(2021)
Review
Cell Biology
Ariadni Androvitsanea, Kostas Stylianou, Eleni Drosataki, Ioannis Petrakis
Summary: Research has shown that heat-shock proteins can have varying effects on the immune system, either triggering immune responses or alleviating them, which is important for studying and treating autoimmune diseases.
Article
Multidisciplinary Sciences
Qiang Li, Zhaowei Zhang, Fengchao Wang, Xiang Wang, Saisong Zhan, Xiaoqing Yang, Chen Xu, Dingbin Liu
Summary: We report a rapid (<30 min) and efficient method for isolating extracellular vesicles (EVs) from diverse biofluids with high yield and purity. The method is based on the reversible zwitterionic coordination between phosphatidylcholine (PC) on EV membranes and PC-inverse choline phosphate (CP) decorated on magnetic beads. By combining this isolation method with proteomics, differentially expressed proteins on the EVs were identified as potential colon cancer biomarkers. Furthermore, the method outperformed conventional approaches in terms of simplicity, speed, yield, and purity for isolating EVs from clinically relevant biofluids such as blood serum, urine, and saliva.
Article
Biochemistry & Molecular Biology
Rachel E. Lackie, Abdul R. Razzaq, Sali M. K. Farhan, Lily R. Qiu, Gilli Moshitzky, Flavio H. Beraldo, Marilene H. Lopes, Andrzej Maciejewski, Robert Gros, Jue Fan, Wing-Yiu Choy, David S. Greenberg, Vilma R. Martins, Martin L. Duennwald, Jason P. Lerch, Hermona Soreq, Vania F. Prado, Marco A. M. Prado
JOURNAL OF NEUROCHEMISTRY
(2020)
Article
Genetics & Heredity
G. S. Kinker, L. H. Ostrowski, P. A. C. Ribeiro, R. Chanoch, S. M. Muxel, I. Tirosh, G. Spadoni, S. Rivara, V. R. Martins, T. G. Santos, R. P. Markus, P. A. C. M. Fernandes
Summary: Melatonin has been found to have an anticancer effect on brain tumors with a negative correlation between its synthesis in the microenvironment and tumor malignancy. MT1 activation inhibits the proliferation of glioma and medulloblastoma cell lines, while MT2 activation promotes it. Tumors with a higher MT1/MT2 expression ratio show better survival rates.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Review
Cell Biology
Glaucia Noeli Maroso Hajj, Paula Borzino Cordeiro Nunes, Martin Roffe
Summary: Gliomas, particularly glioblastoma, exhibit deregulation of translational control due to overactivation of signaling pathways such as PI3K/AKT/mTORC1 and Ras/ERK1/2. Transcriptome analysis can provide important insights into gene expression patterns in gliomas.
CELLULAR SIGNALLING
(2021)
Review
Oncology
Celso Abdon Mello, Fernando Augusto Batista Campos, Tiago Goss Santos, Maria Leticia Gobo Silva, Giovana Tardin Torrezan, Felipe D'Almeida Costa, Maria Nirvana Formiga, Ulisses Nicolau, Antonio Geraldo Nascimento, Cassia Silva, Maria Paula Curado, Suely Akiko Nakagawa, Ademar Lopes, Samuel Aguiar Jr
Summary: Desmoplastic small round cell tumor (DSRCT) is a rare and aggressive sarcoma primarily affecting adolescents and young adults, with a male predominance. Current treatment strategies include chemotherapy, radiation, aggressive surgery, and intra-peritoneal hyperthermic chemotherapy, but high recurrence rates and poor survival outcomes necessitate the urgent development of new therapeutic options. Ongoing research is focused on identifying potential therapeutic targets through comprehensive molecular profiling to improve understanding of DSRCT biology and explore novel treatment strategies in preclinical and early phase clinical trials.
Article
Dentistry, Oral Surgery & Medicine
Eloa Mangabeira Santos, Carlos Alberto de Carvalho Fraga, Alessandra Rejane Ericsson de Olive Xavier, Mauro Aparecido de Souza Xavier, Marcela Goncalves Souza, Sabrina Ferreira de Jesus, Alfredo Mauricio Batista de Paula, Lucyana Conceicao Farias, Sergio Henrique Sousa Santos, Tiago Goss Santos, Flavio H. Beraldo, Andre Luiz Sena Guimaraes
Summary: This study revealed the association between hypoxia-inducible factor and heat shock proteins with PRNP expression, leading to the promotion of lymph node metastasis in head and neck squamous cell carcinoma. These findings provide a molecular basis for the role of PRNP in HNSC progression.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2021)
Review
Oncology
J. Y. Blay, N. Hindi, J. Bollard, S. Aguiar Jr, M. Angel, B. Araya, R. Badilla, D. Bernabeu, F. Campos, C. H. S. Caro-Sanchez, B. Carvajal, A. Carvajal Montoya, S. Casavilca-Zambrano, V Castro-Oliden, M. Chacon, M. Clara, P. Collini, R. Correa Genoroso, F. D. Costa, M. Cuellar, A. P. dei Tos, H. R. Dominguez Malagon, D. Donati, A. Dufresne, M. Eriksson, M. Farias-Loza, P. Fernandez, A. M. Frezza, T. Frisoni, D. Y. Garcia-Ortega, H. Gelderblom, F. Gouin, M. C. Gomez-Mateo, A. Gronchi, J. Haro, L. Huanca, N. Jimenez, M. Karanian, B. Kasper, B. B. Lopes David, A. Lopez-Pousa, G. Lutter, H. Martinez-Said, J. Martinez-Tlahuel, C. A. Mello, J. M. Morales Perez, S. Moura David, A. G. Nascimento, E. J. Ortiz-Cruz, E. Palmerini, S. Patel, Y. Pfluger, S. Provenzano, A. Righi, A. Rodriguez, R. Salas, T. T. G. Santos, K. Scotlandi, T. Soule, S. Stacchiotti, C. Valverde, F. Waisberg, E. Zamora Estrada, J. Martin-Broto
CANCER TREATMENT REVIEWS
(2022)
Article
Oncology
Daniel Antunes Moreno, Luciane Sussuchi da Silva, Maicon Fernando Zanon, Murilo Bonatelli, Flavia Escremim de Paula, Marcus de Medeiros Matsushita, Gustavo Ramos Teixeira, Iara Viana Vidigal Santana, Fabiano Saggioro, Luciano Neder, Joao N. Stavale, Suzana Maria Fleury Malheiros, Matheus Lima, Glaucia Noeli Maroso Hajj, Hernan Garcia-Rivello, Silvia Christiansen, Susana Nunes, Maria Joao Gil da Costa, Maria Jose Soares, Jorge Pinheiro, Carlos Almeida Junior, Bruna Minniti Mancano, Rui Manuel Reis
Summary: The study successfully stratified medulloblastoma molecular subgroups and predicted MYCN amplification using a single nCounter assay without the need for additional biological tissue, costs, or bench time.
JOURNAL OF NEURO-ONCOLOGY
(2022)
Review
Oncology
J. Y. Blay, E. Palmerini, J. Bollard, S. Aguiar Jr, M. Angel, B. Araya, R. Badilla, D. Bernabeu, F. Campos, C. H. S. Caro-Sanchez, A. Carvajal Montoya, S. Casavilca-Zambrano, V Castro-Oliden, M. Chacon, M. A. Clara-Altamirano, P. Collini, R. Correa Genoroso, F. D. Costa, M. Cuellar, A. P. dei Tos, H. R. Dominguez Malagon, D. M. Donati, A. Dufresne, M. Eriksson, M. Farias-Loza, A. M. Frezza, T. Frisoni, D. Y. Garcia-Ortega, H. Gerderblom, F. Gouin, M. C. Gomez-Mateo, A. Gronchi, J. Haro, N. Hindi, L. Huanca, N. Jimenez, M. Karanian, B. Kasper, A. Lopes, B. B. Lopes David, A. Lopez-Pousa, G. Lutter, R. G. Maki, H. Martinez-Said, J. L. Martinez-Tlahuel, C. A. Mello, J. M. Morales Perez, D. S. Moura, S. A. Nakagawa, A. G. Nascimento, E. J. Ortiz-Cruz, S. Patel, Y. Pfluger, S. Provenzano, A. Righi, A. Rodriguez, T. G. Santos, K. Scotlandi, M. L. G. Silva, T. Soule, S. Stacchiotti, C. M. Valverde, F. Waisberg, E. Zamora Estrada, J. Martin-Broto
Summary: Bone sarcoma is a rare disease with a low incidence in adult population. The multidisciplinary management and expert discussion of diagnostic and therapeutic strategies are essential for these patients. The Sarcoma European Latin-American Network (SELNET) aims to improve clinical outcomes in sarcoma care, with a specific focus on Latin-American countries. The Clinical Practice Guidelines (CPG) developed by SELNET provide a standardized approach to diagnose, treat, and follow-up bone sarcoma patients in the Latin-American context.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Najla Santos Pacheco de Campos, Adriano de Oliveira Beserra, Pedro Henrique Barbosa Pereira, Alexandre Silva Chaves, Fernando Luiz Affonso Fonseca, Tiago da Silva Medina, Tiago Goss dos Santos, Yufei Wang, Wayne Anthony Marasco, Eloah Rabello Suarez
Summary: This study found that CD28-based anti-CAIX CAR PBMCs cells, releasing anti-PD-L1 IgG, showed superior antitumor effects in vivo, especially under the influence of PD-L1 via ICB. The exhaustion status of CD4 T cells, in addition to CD8 T cells, was critical for the efficacy of CAR T cells. These findings offer exciting new prospects for the treatment of refractory ccRCC and hypoxic tumors using anti-CAIX CAR T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Lara Vecchi, Sara Teixeira Soares Mota, Mariana Alves Pereira Zoia, Isabella Castro Martins, Jessica Brito de Souza, Tiago Goss Santos, Adriano de Oliveira Beserra, Victor Piana de Andrade, Luiz Ricardo Goulart, Thaise Goncalves Araujo
Summary: Annexin A1 and interleukin-6 are both overexpressed in breast cancer tissue, indicating a correlation between the two. The activation of the AnxA1/FPR1 autocrine axis plays a crucial role in the IL-6 signaling pathway and has an impact on the tumor microenvironment.
Article
Cell Biology
Ana Claudia Munhoz, Julian D. C. Serna, Eloisa Aparecida Vilas-Boas, Camille C. Caldeira C. da Silva, Tiago G. Santos, Francielle C. Mosele, Sergio L. Felisbino, Vilma Regina Martins, Alicia J. Kowaltowski
Summary: Obesity decreases life expectancy and increases age-related dysfunctions, including beta-cell dysregulation. Diluted plasma from obese human donors and sera from obese rats impair beta-cell integrity and insulin secretion, while the presence of adiponectin, a protective characteristic found in lean plasma, restores beta-cell function. Low circulating adiponectin is a key damaging element for beta-cells and modulation of the adiponectin signaling pathway could be a potential therapy for preventing age-related beta-cell dysfunction.
Article
Biochemistry & Molecular Biology
Fernanda F. da Silva, Fernanda C. S. Lupinacci, Bruno D. S. Elias, Adriano O. Beserra, Paulo Sanematsu, Martin Roffe, Leslie D. Kulikowski, Felipe D'almeida Costa, Tiago G. Santos, Glaucia N. M. Hajj
Summary: This study successfully established and characterized the GBM33 cell line, which belongs to the GBM class and exhibits similar characteristics and functions as GBM cells. Compared to commercial cell lines, GBM33 cells show increased growth rate and similar sensitivity to temozolamide, and they can produce primary cilia upon serum starvation. The establishment of this cell line provides valuable tools for studying GBM biology, identifying therapeutic targets, and evaluating treatment efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Adriano O. Beserra, Ethiene C. Estevan, Stephania M. Bezerra, Giovana T. Torrezan, Amanda Ikegami, Humberto Delle, Isabela W. Cunha, Isabella T. Meira, Dirce M. Carraro, Primo N. Lara, Stenio C. Zequi, Vilma R. Martins, Tiago G. Santos
Summary: This study aimed to establish a renal cell carcinoma (RCC) patient-derived xenograft (PDX) platform to identify novel clinical and molecular biomarkers of recurrence risk. Orthotopic implantation was found to be more suitable for establishing the PDX model, preserving RCC identity and major molecular alterations. The capacity of tumor engraftment was also found to be a strong prognostic indicator for patients with locally advanced disease.
Review
Respiratory System
Glaucia N. M. Hajj, Carolina H. Cavarson, Clovis Antonio Lopes Pinto, Gabriela Venturi, Joao R. Navarro, Vladmir C. Cordeiro de Lima
Summary: Malignant mesotheliomas are rare cancers affecting mesothelial surfaces, often associated with asbestos exposure. Due to a long latency period and difficult diagnosis, most cases are detected in advanced stages with limited treatment options and a short survival period. This review discusses the molecular physiopathology, current diagnosis, and latest therapeutic options for this disease.
JORNAL BRASILEIRO DE PNEUMOLOGIA
(2021)