Article
Plant Sciences
Yu Tian, Kankan Peng, Guicheng Lou, Zhipeng Ren, Xianze Sun, Zhengwei Wang, Jinpu Xing, Chunhua Song, Jing Cang
Summary: This study is the first to explore the transcriptome of Dn1 under cold stress, and comprehensively analyzed the key genes involved in cold signal transduction and carbohydrate metabolism in Dn1 under cold stress.
Article
Forestry
Gaoman Linghu, Zhaoyou Yu, Meng Li, Anqi Wang, Yongxiang Kang
Summary: Davidia involucrata is a rare and endangered plant species with low reproductive efficiency. Somatic embryogenesis, a biotechnological method, offers a solution to improve the reproductive efficiency of D. involucrata. RNA sequencing analysis revealed that certain hormone-related gene families were upregulated in embryogenic callus compared to non-embryogenic callus, indicating their crucial roles in cell proliferation, differentiation, and totipotency in D. involucrata. These findings provide new insights into improving the induction, culture conditions, and genetic transformation efficiency of somatic embryogenesis in D. involucrata.
Article
Biochemistry & Molecular Biology
Hongcheng Wang, Lvlan Ye, Lizhou Zhou, Junxing Yu, Biao Pang, Dan Zuo, Lei Gu, Bin Zhu, Xuye Du, Huinan Wang
Summary: Soil salinization is a serious problem that affects crop yield and quality. This study investigated the gene expression profiles of sorghum under alkaline salt stress using RNA sequencing. The results revealed that genes related to plant hormone signal transduction, MAPK signaling pathway, starch and sucrose metabolism, glutathione metabolism, and phenylpropanoid biosynthesis were significantly differentially expressed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yun Wang, Xun Sun, Zhenwu Zhang, Bisheng Pan, Wenyu Xu, Shaoling Zhang
Summary: Ring rot disease caused by B. dothidea is a serious issue for the pear industry, with unclear understanding of the interactions between pear and pathogens. Through transcriptome analysis, many differentially expressed genes related to plant-pathogen interactions and hormone signal transduction were identified during B. dothidea infection. The discovery of the significant role of PbrPUB29 in pear disease resistance provides new insights for molecular mechanisms underlying pear-pathogen interactions and potential disease resistance strategies.
Article
Biochemistry & Molecular Biology
Huidong Xuan, Yanzhong Huang, Li Zhou, Sushuang Deng, Congcong Wang, Jianyu Xu, Haitang Wang, Jinming Zhao, Na Guo, Han Xing
Summary: In this study, comprehensive comparative transcriptome analyses were conducted to explore the molecular mechanisms of soybean drought tolerance. The results revealed that drought-tolerant genotype Jindou 21 (JD) exhibited better adaptation and drought resistance compared to drought-sensitive genotype Tianlong No.1 (N1) through multiple signal transduction pathways, transcription factors, and stress-related proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Haragopal Dutta, Gyan P. Mishra, Muraleedhar S. Aski, Tejas C. Bosamia, Dwijesh C. Mishra, Jyotika Bhati, Subodh Kumar Sinha, Dunna Vijay, Manjunath C. T. Prasad, Shouvik Das, Prashant Anupama-Mohan Pawar, Atul Kumar, Kuldeep Tripathi, Ranjeet Ranjan Kumar, Devendra Kumar Yadava, Shiv Kumar, Harsh Kumar Dikshit
Summary: This study investigated the transcriptome of large-seeded and small-seeded lentil genotypes to unravel the pathways regulating seed size. Differentially expressed genes associated with hormone signaling, cell division, and transcription factors were identified. Additionally, several microRNAs related to growth and cell division were found to play a role in seed size determination. EST-SSR primers were generated for future marker identification. The study provides insights into the key seed size regulatory pathways in lentils.
FRONTIERS IN GENETICS
(2022)
Article
Environmental Sciences
Shouping Zhao, Qi Zhang, Wendan Xiao, De Chen, Jing Hu, Na Gao, Miaojie Huang, Xuezhu Ye
Summary: Cd is still a major contaminant in rice production. Study on the mechanism of genotypic differences in Cd enrichment in rice is necessary to regulate Cd enrichment. Two rice cultivars (low Cd, ZZ143; and high Cd, YX409) showed different transcriptomic profile patterns under 100 μmol/L Cd stress. The identified DEGs and enriched pathways suggest that ZZ143 has a stronger ability for sulfur assimilation and Cys synthesis, while YX409 has a stronger ability to maintain cell wall stability, which may contribute to their different Cd concentrations.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Multidisciplinary Sciences
Xu Shi, Xiao Wang, Andrew F. Neuwald, Leena Halakivi-Clarke, Robert Clarke, Jianhua Xuan
Summary: BayesDenovo addresses the challenge of de novo transcriptome assembly by accurately reconstructing splicing graphs using a read-guided and Bayesian strategy, achieving higher accuracy and reducing false positives, especially for alternatively spliced genes and highly/lowly expressed transcripts. Additionally, it is more robust on multiple replicates and can identify phenotype-specific transcripts associated with breast cancer recurrence.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Ikuko Yuyama, Naoto Ugawa, Tetsuo Hashimoto
Summary: By comparing transcriptome data from free-living and symbiotic Durusdinium, significant gene expression changes related to heat-shock proteins, photosynthesis, and antibiotic synthesis were identified, providing insights into the genetic regulation during coral-dinoflagellate endosymbiosis establishment.
Article
Multidisciplinary Sciences
August Guang, Mark Howison, Felipe Zapata, Charles Lawrence, Casey W. Dunn
Summary: treeinform is a method that utilizes phylogenetic information across species to improve transcriptome assemblies within species. It helps identify and correct the error of mistaking different transcripts of the same gene as transcripts from multiple closely related genes.
Article
Biochemical Research Methods
Qianqian Zhang, Xing Xu, Li Lin, Jian Yang, Xing Na, Xin Chen, Lingling Wu, Jia Song, Chaoyong Yang
Summary: Cilo-seq is a high-performance platform for single-cell RNA sequencing library construction. It utilizes digital microfluidics in a single device, enabling convenient single-cell isolation, efficient nucleic acid amplification, low-loss nucleic acid purification, and high-quality library preparation.
Article
Plant Sciences
Md. Shofiqul Islam, Nick Krom, Taegun Kwon, Guifen Li, Malay C. Saha
Summary: This study identified differentially expressed genes in tall fescue under different temperatures through RNA-seq analysis. The results showed that there were fewer differentially expressed genes in the morning freezing conditions and more in the evening cold conditions. Additionally, the study found that the cold tolerance of tall fescue might be related to some orthologous genes of rice.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Chaoqiong Ding, Xiang Yan, Mengying Xu, Ran Zhou, Yuancun Zhao, Dan Zhang, Zongyao Huang, Zhenzhong Pan, Peng Xiao, Huifang Li, Lu Chen, Yuan Wang
Summary: This study reports the full-length transcriptome of mouse neural stem cells (NSCs) at different developmental stages, providing an important resource for studying NSC fate determination, neural development, and disease in the neuroscience community.
Article
Biotechnology & Applied Microbiology
Syed Mudasir Ahmad, Sahar Saleem Bhat, Shaista Shafi, Mashooq Ahmad Dar, Afnan Saleem, Zulfqarul Haq, Nida Farooq, Junaid Nazir, Basharat Bhat
Summary: Using three different chicken breeds, the study found that NF-kappa B1, FOXO3, and PaX5 genes were upregulated in Kashmir favorella chickens after Salmonella infection, with FOXO3 potentially serving as a marker for host resistance to Salmonella. This study contributes to the development of new strategies for treating and preventing Salmonella infection, as well as improving innate disease resistance in chickens.
Article
Plant Sciences
Lin Lin, Jie Wang, Qun Wang, Mengcheng Ji, Sidan Hong, Linxue Shang, Guozhe Zhang, Yu Zhao, Qingqing Ma, Cuihua Gu
Summary: In this study, the molecular mechanism of drought resistance in Heimia myrtifolia was investigated through transcriptome analysis. The significantly enriched pathways included photosynthesis, plant hormone signal transduction, and others. Transcription factors and related genes on these pathways played important roles in the drought process.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Physical
Xiaowei Xu, Yong Zhou, Linlin Liu, Peng Guo, Xiaowei Li, Kwang-Ryeol Lee, Ping Cui, Aiying Wang
APPLIED SURFACE SCIENCE
(2020)
Article
Chemistry, Physical
Jing Wei, Peng Guo, Linlin Liu, Hanchao Li, Hao Li, Shuyuan Wang, Peiling Ke, Aiying Wang
APPLIED SURFACE SCIENCE
(2020)
Article
Electrochemistry
Jing Wei, Peng Guo, Linlin Liu, Hanchao Li, Hao Li, Shuyuan Wang, Peiling Ke, Hidetoshi Saito, Aiying Wang
ELECTROCHIMICA ACTA
(2020)
Article
Materials Science, Coatings & Films
Dong Zhang, Xiao Zuo, Zhenyu Wang, Hao Li, Rende Chen, Aiying Wang, Peiling Ke
SURFACE & COATINGS TECHNOLOGY
(2020)
Article
Nanoscience & Nanotechnology
Xiaowei Li, Xiaowei Xu, Jianwei Qi, Dekun Zhang, Aiying Wang, Kwang-Ryeol Lee
Summary: The study revealed that the interaction between hydrogenated amorphous carbon oil/graphene nano-composite interfaces at the atomic scale. Results suggest that optimizing the hydrogen content of mated a-C:H surfaces is crucial for achieving superlow friction or even superlubricity. The fundamental friction mechanism is attributed to hydrogenation-induced passivation of the friction interface and the squeezing effect on the fluid lubricant.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Physical
Hao Li, Yang Xin, Keiji Komatsu, Peng Guo, Guanshui Ma, Peiling Ke, Kwang-Ryeol Lee, Hidetoshi Saito, Aiying Wang
Summary: In this study, the relationship among the plasma state, microstructure, and performance of amorphous carbon-coated 316L stainless steel in proton exchange-membrane fuel cells was studied by adjusting the working pressure. Results showed that high ionization degree of the plasma resulted in high compactness and large sp(2) cluster size, leading to the best performance. On the other hand, low ionization degree caused loose structure and serious interface damage.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Chemistry, Physical
Guanshui Ma, Yapeng Zheng, Jiayue Zhang, Jiangshan Yan, Peng Guo, Wei Yang, Rende Chen, Jianghuai Yuan, Li Cui, Aiying Wang
Summary: Mo-NiMo/NF electrodes were prepared by high power impulse magnetron sputtering and subsequent annealing, and exhibited exceptional electrocatalytic performance and stability in alkaline aqueous electrolyte. The catalytic activity of the electrodes was attributed to the NiMo alloy and the coupling effect between Mo and NiMo. The scalable synthesis method reported in this study provides a valuable strategy for improving the catalytic activity of electrocatalysts.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Review
Materials Science, Multidisciplinary
Yingpeng Zhang, Qun Wang, Chidambaram Seshadri Ramachandran, Peng Guo, Aiying Wang
Summary: The paper summarizes the current development of high-velocity oxygen-fuel coupled physical vapor deposition (HVOF-PVD) duplex coatings as protective candidates. The fabrication methods for duplex protective coatings are presented, and the relation between structural characteristics and performance is described. It is demonstrated that the duplex coatings show more excellent overall performance than coatings deposited by both HVOF and PVD separately. The protective performance and promising potential of HVOF-PVD duplex coating for applications are summarized, along with the research prospects of challenges in future.
Article
Materials Science, Ceramics
Yupeng Zhang, Zhenyu Wang, Peng Guo, Aiying Wang, Peiling Ke
Summary: In this study, a DC magnetron sputtered VAlN hard ceramic coating was modified by post-annealing treatment with graphite powder, resulting in an intermittent amorphous carbon (a-C) top-layer. The modified coating exhibited high hardness (34.4 GPa) and excellent self-adaptive lubrication from room temperature to 600 degrees C. The low friction coefficient (0.11) at 300 degrees C was attributed to the lamellar sliding interface between the a-C top-layer and the a-C transfer film (30 nm-68 nm), while the increased and stabilized friction coefficient (0.36-0.41) at 600 degrees C was attributed to the formation of V2O5 lubricating phase. The tribological behavior of the modified VAlN coating by a-C top-layer was further analyzed in terms of microstructure evolution during friction test.
CERAMICS INTERNATIONAL
(2023)
Article
Materials Science, Multidisciplinary
Jianghuai Yuan, Zhenyu Wang, Guanshui Ma, Xiaojing Bai, Yong Li, Xiaoying Cheng, Peiling Ke, Aiying Wang
Summary: The unique nano-laminated structures of Mn+1AXn (MAX) phase provide ceramics and metals with interesting hybrid properties, making it an attractive potential protective coating in harsh systems. However, the narrow phase-forming region makes it challenging to obtain high-purity MAX phase coatings with high-temperature anti-oxidation capabilities. This study investigates the phase evolution of M-Al-C (M = Ti, V, Cr) coatings as a function of temperature using in-situ X-ray diffraction analysis.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Materials Science, Ceramics
Zheng Wang, Wentao Li, Zhenyu Wang, Ming Li, Aiying Wang, Peiling Ke
Summary: After the Fukushima nuclear accident, the concept of accident-tolerant fuel (ATF) cladding coating was proposed to increase rescue time. Chromium (Cr) coatings are considered promising materials for ATF cladding under loss-of-coolant-accident conditions. The oxidation behavior of Cr coatings is influenced by the microstructure and defects formed during the deposition process. High-impulse-power magnetron sputtering (HiPIMS) was used to deposit Cr coatings on Zirlo substrates, resulting in improved steam oxidation resistance compared to traditional arc ion plating (AIP) technique.
CERAMICS INTERNATIONAL
(2023)
Article
Materials Science, Multidisciplinary
Dingwei Zhou, Zhenyu Wang, Yan Zhang, Jingjun Yan, Guanshui Ma, Xiaojun Hu, Peiling Ke, Aiying Wang
Summary: This study demonstrates that the multi-layer TiAl-N coating provides excellent protection for marine engines, despite some coating spallation and delamination during corrosion.
Article
Physics, Multidisciplinary
Guangxue Zhou, Xiao Zuo, Rende Chen, Zhenyu Wang, Peiling Ke, Aiying Wang
Summary: In this study, ultrathick and compact Cr coatings with (001) preferential orientation were deposited on Zircaloy-4 alloy using the HiPIMS technique. The growth characteristics of the Cr coatings were investigated by changing the discharge modes, resulting in a rapid increase in Cr+ content and enhanced incident energy flux to the coating. A simplified collision model was proposed to understand the effect of HiPIMS Cr plasma on achieving nanocrystalline Cr coatings with ultra-thick and dense structures.
Article
Multidisciplinary Sciences
Sixie Zhang, Yunan Wang, Shuyu Li, Zhongfeng Wang, Haocheng Chen, Li Yi, Xu Chen, Qihao Yang, Wenwen Xu, Aiying Wang, Zhiyi Lu
Summary: It has been found that bromide ions in seawater are more harmful to nickel anodes due to inferior corrosion resistance and faster corrosion kinetics compared to chloride ions. Bromide ions generate extensive shallow-wide pits, while chloride ions form narrow-deep pits. In addition, bromide ions cause extensive spalling of catalyst layers on Ni-based electrodes. Therefore, designing anti-bromide corrosion anodes is crucial for future applications of seawater electrolysis.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Shuyu Li, Hao Li, Guanshui Ma, Jing Wei, Guangxue Zhou, Yan Zhang, Peng Guo, Peiling Ke, Aiying Wang
Summary: Dense Cr/GLC multilayer coatings fabricated by high power impulse magnetron sputtering (HiPIMS) show improved corrosion resistance with lower corrosion current density and excellent long-term corrosion resistance.