Article
Nanoscience & Nanotechnology
Zhuoyue Lei, Hongwei Jiang, Jie Liu, Yuping Liu, Di Wu, Chenwei Sun, Qijun Du, Liangwen Wang, Guohua Wu, Shuqi Wang, Xingdong Zhang
Summary: This study presents a novel method to enhance the biofabrication of cancer-derived extracellular vesicles (EVs) using audible acoustic wave (AAW), which significantly increases EV yield and promotes cancer cell migration and invasion. Additionally, AAW stimulation activates the ESCRT pathway and upregulates membrane fusion-associated proteins, contributing to the enhancement of EV biofabrication.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Caroline Procopio de Oliveira, Barbara Maria Frigieri, Heidge Fukumasu, Luiz Gustavo de Almeida Chuffa, Adriana Alonso Novais, Debora Aparecida Pires de Campos Zuccari
Summary: Melatonin shows a protective effect in the clinical treatment of canine mammary tumors by modulating EV-mediated signaling.
Review
Biotechnology & Applied Microbiology
Jiangbin Chen, Qi Tan, Zimo Yang, Yang Jin
Summary: Extracellular vesicles (EVs) are secretory vesicles with cell-derived membrane and contents, which can be designed as drug delivery platforms for cancer therapy due to their cargo delivery capability. With biocompatibility and immune compatibility, EVs have unique advantages compared with other nanocarriers. Engineered EVs can be modified to achieve desired performance and have potential for various combinations of cancer treatment methods. Application of engineered EVs in cancer therapy is shifting towards combinational therapy to combat heterogeneous cancer cells and complex tumor microenvironment, highlighting the need for more effective treatment plans in the future.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rui Miguel Marques Bernardino, Ricardo Leao, Rui Henrique, Luis Campos Pinheiro, Prashant Kumar, Prashanth Suravajhala, Hans Christian Beck, Ana Sofia Carvalho, Rune Matthiesen
Summary: The study explores possible strategies for urinary biomarker discovery, especially focused on proteome of urine EVs for PCa. Through reanalysis of proteomics data and network analysis, the findings suggest that urinary EVs may provide potential biomarkers with higher protein coverage compared to other sample sources and may fully characterize the heterogeneity of PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Xiangnan Zhao, Yue Liu, Pingping Jia, Hui Cheng, Chen Wang, Shang Chen, Haoyan Huang, Zhibo Han, Zhong-Chao Han, Krzysztof Marycz, Xiaoniao Chen, Zongjin Li
Summary: The study demonstrated that chitosan hydrogel-encapsulated extracellular vesicles (EVs) could effectively rejuvenate aging dermal fibroblasts by promoting cell proliferation, enhancing ECM protein synthesis, and inhibiting MMPs overexpression. Furthermore, subcutaneous injection of CS-EVs in a mouse model significantly delayed skin aging processes, showcasing the potential of this novel therapeutic approach for rejuvenating aging skin.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Jiani Liu, Xinyu Peng, Yang Yang, Yao Zhang, Meng Han, Xiaohui Shi, Jie Zheng, Tong Li, Jinxia Chen, Weihua Lv, Yunjiang Liu, Yixin Qi, Lei Zhang, Qi Liu
Summary: The study found that hsa_circ_0058514 can serve as an early plasma biomarker for breast cancer diagnosis with better diagnostic value than conventional tumor markers. It is also a predictor for the efficacy of neoadjuvant chemotherapy. Additionally, the expression level of hsa_circ_0058514 is associated with clinicopathological characteristics in breast cancer patients.
FRONTIERS IN ONCOLOGY
(2022)
Review
Medicine, General & Internal
Fengyi Yu, Yingjie Duan, Chongmei Liu, Hong Huang, Xiangcheng Xiao, Zhangxiu He
Summary: Atherosclerosis (AS) is characterized by the accumulation of lipids, fibrous elements, and calcification in the innermost layers of arteries. Vascular calcification (VC), the deposition of calcium and phosphate within the arterial wall, is an important characteristic of AS natural history. Endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) play critical roles in AS and VC, and the understanding of their communication is crucial in revealing the underlying mechanisms. Extracellular vesicles (EVs) contain non-coding RNAs (ncRNAs) that are involved in AS and VC, but the functions of the majority of ncRNAs encapsulated in EVs remain unclear. This review summarizes the ncRNAs encapsulated in EC-EVs and VSMC-EVs, as well as the signaling pathways involved in AS and VC.
FRONTIERS IN MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Bianca Cruz Pachane, Ana Carolina Caetano Nunes, Thais Regiani Cataldi, Kelli Cristina Micocci, Bianca Caruso Moreira, Carlos Alberto Labate, Heloisa Sobreiro Selistre-de-Araujo, Wanessa Fernanda Altei
Summary: Hypoxia in triple-negative breast tumors promotes the secretion of extracellular vesicles, which enhance cell invasion. In this study, we investigated the invasive properties triggered by hypoxic small EVs derived from TNBC tumors. The results showed that hypoxic EVs have increased protein abundance and diversity, and are enriched in pro-invasive pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
David Rufino-Ramos, Sevda Lule, Shadi Mahjoum, Stefano Ughetto, D. Cristopher Bragg, Luis Pereira de Almeida, Xandra O. Breakefield, Koen Breyne
Summary: The lack of techniques to trace brain cell behavior limits the ability to monitor their status. Extracellular vesicles (EVs), released by brain cells, might be able to report their status. However, identifying brain-derived EVs in complex biofluids is challenging. This study developed a sensitive platform to isolate pre-labelled brain-derived EVs from blood, allowing for the study of molecular fingerprints of brain cells.
Review
Cell Biology
Jancy Johnson, Mozhgan Shojaee, James Mitchell Crow, Ramin Khanabdali
Summary: MSCs, as a leading cell source for stem cell therapy, have regenerative and immunomodulatory properties, but face challenges in clinical applications. EVs and EEVs, as potential cell-free alternatives, may overcome these challenges in the clinical application of MSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Clinical Neurology
Victor Bodart-Santos, Lisandra S. Pinheiro, Almir J. da Silva-Junior, Rudimar L. Froza, Rosemary Ahrens, Rafaella A. Goncalves, Mayara M. Andrade, Yan Chen, Carolina de Lima Alcantara, Lea T. Grinberg, Renata E. P. Leite, Sergio T. Ferreira, Paul E. Fraser, Fernanda G. De Felice
Summary: The study found that extracellular vesicles (EVs) in Alzheimer's disease (AD) and frontotemporal dementia (FTD) not only contribute to the spread of neuropathology, but also have an impact on behavioral outcomes associated with AD. Memory tests were conducted by injecting EVs isolated from post mortem brain tissue from control, AD, or FTD donors, as well as from APP/PS1 mice, into the hippocampi of wild-type or a humanized Tau mouse model. The results showed that AD-EVs and FTD-EVs carry Tau protein, have altered protein composition related to synapse regulation and transmission, and induce memory impairment.
ALZHEIMERS & DEMENTIA
(2023)
Review
Medicine, General & Internal
Magdalena Kaczmarek, Monika Baj-Krzyworzeka, Lukasz Bogucki, Magdalena Dutsch-Wicherek
Summary: Cervical cancer, primarily caused by human papillomavirus (HPV) infection, is influenced by the persistence of the virus in infected cells. Extracellular vesicles (EVs), which contain viral components, could serve as a mode of cell-to-cell communication, potentially impacting the microenvironment of cervical cancer and its metastatic potential.
Article
Biology
Dovydas Gecys, Arunas Kazlauskas, Emilija Gecyte, Neringa Pauziene, Deimante Kulakauskiene, Indre Lukminaite, Aiste Jekabsone
Summary: This study explores the potential of genetically engineered extracellular vesicles to improve drug delivery in glioblastoma cells. The results show that introducing the RGD ligand significantly increases the internalisation of extracellular vesicles by GBM cells, leading to improved drug delivery efficacy.
Article
Engineering, Biomedical
Marie J. Pauwels, Junhua Xie, Adam Ceroi, Sriram Balusu, Jonas Castelein, Elien Van Wonterghem, Griet Van Imschoot, Andrew Ward, Trevelyan R. Menheniott, Oskar Gustafsson, Francis Combes, Samir El Andaloussi, Niek N. Sanders, Imre Mager, Lien Van Hoecke, Roosmarijn E. Vandenbroucke
Summary: The brain is protected by tightly regulated barriers, which impede drug delivery. Extracellular vesicles derived from choroid plexus epithelial cells have the potential to serve as drug delivery vehicles to bypass these barriers.
Article
Biochemistry & Molecular Biology
Luciana M. M. Dominguez, Barbara Bueloni, Ma. Jose Cantero, Milagros Albornoz, Natalia Pacienza, Celeste Biani, Carlos Luzzani, Santiago Miriuka, Mariana Garcia, Catalina Atorrasagasti, Gustavo Yannarelli, Juan Bayo, Esteban Fiore, Guillermo Mazzolini
Summary: Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have been shown to have similar phenotype and antifibrotic activity when isolated from adipose tissue MSCs, induced-pluripotent-stem-cell-derived MSCs, and umbilical cord perivascular cells (HUCPVC) using ion exchange chromatography. EVs derived from AdhIGF-I-HUCPVC carried IGF-1 and exhibited a higher therapeutic effect in vitro and in vivo. This scalable manufacturing strategy of MSC-derived EVs holds promise as a therapeutic tool for liver fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Gabriella Schiera, Carlo Maria Di Liegro, Italia Di Liegro
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Marco Giammanco, Carlo Maria Di Liegro, Gabriella Schiera, Italia Di Liegro
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Genetics & Heredity
Fabio Caradonna, Gabriella Schiera, Carlo Maria Di Liegro, Vincenzo Vitale, Ilenia Cruciata, Tiziana Ferrara, Pietro D'Oca, Riccardo Mormino, Simona Maria Angela Rizzo, Italia Di Liegro
Review
Oncology
Gabriella Schiera, Carlo Maria Di Liegro, Italia Di Liegro
Summary: The development and function of the mammalian brain depends on precise regulation of gene expression by thyroid hormones, which act at multiple levels and cell types. Thyroid hormones have both genomic and non-genomic effects on brain cells, affecting proliferation, differentiation, and metabolism. Furthermore, thyroid hormone levels may influence cancer proliferation and invasion through complex interactions with their targets.
Article
Cell Biology
Yuta Kanamori, Alessia Finotti, Laura Di Magno, Gianluca Canettieri, Tomoaki Tahara, Fabio Timeus, Antonio Greco, Paola Tirassa, Jessica Gasparello, Pasquale Fino, Carlo Maria Di Liegro, Patrizia Proia, Gabriella Schiera, Italia Di Liegro, Roberto Gambari, Enzo Agostinelli
Summary: Neuroblastoma is a common malignant solid tumor in children, with N-Myc amplification being a poor prognostic marker in NB patients. Treatment with BSAO/SPM leads to increased mRNA levels of proapoptotic genes in NB cell lines, inducing apoptosis activity.
Article
Nutrition & Dietetics
Alessandra Amato, Paolo Ragonese, Sonia Ingoglia, Gabriella Schiera, Giuseppe Schiro, Carlo Maria Di Liegro, Giuseppe Salemi, Italia Di Liegro, Patrizia Proia
Summary: This study investigated the effects of lactacid anaerobic training on multiple sclerosis patients. The results showed that the exercise influenced BDNF and DHEAS levels, reduced baseline lactate levels, and increased self-efficacy and self-esteem in the patients.
Article
Genetics & Heredity
Gabriella Schiera, Patrizia Cancemi, Carlo Maria Di Liegro, Flores Naselli, Sara Volpes, Ilenia Cruciata, Paola Sofia Cardinale, Fabiola Vaglica, Matteo Calligaris, Anna Paola Carreca, Roberto Chiarelli, Simone Dario Scilabra, Olga Leone, Fabio Caradonna, Italia Di Liegro
Summary: Gliomas, especially astrocytomas, are the most frequent brain cancers, derived from glial cells. Astrocytes play essential roles in brain functions, but when they become cancerous, their functions are altered and they invade the brain tissue. Studying the molecular properties of transformed astrocytes is crucial for better understanding of this cancer.
Review
Biochemistry & Molecular Biology
Carlo Maria Di Liegro, Gabriella Schiera, Giuseppe Schiro, Italia Di Liegro
Summary: The post-transcriptional regulation of mRNA fate plays a central role in nervous system development and function. RNA-binding proteins (RBPs) control the maturation, localization, stability, and translation of transcripts by recognizing and binding RNA sequences, base modifications, or secondary/tertiary structures. RBPs, which often contain intrinsically disordered regions (IDRs), are involved in the formation of membrane-less structures and can form aggregates under altered cellular conditions. Additionally, RBPs can enter extracellular vesicles (EVs), allowing them to be transported to cells other than those that produced them.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)