Article
Engineering, Biomedical
Chao Sun, Yinhua Dong, Jun Wei, Meng Cai, Dongfang Liang, Yongqing Fu, You Zhou, Yi Sui, Fangda Wu, Roman Mikhaylov, Hanlin Wang, Feifei Fan, Zhihua Xie, Mercedes Stringer, Zhiyong Yang, Zhenlin Wu, Liangfei Tian, Xin Yang
Summary: This study develops a highly effective acoustic stimulator working at -20 MHz to investigate the roles of acousto-mechanical stimuli in the differentiation of hESCs. Our results show that acoustic dose alters the expression of extracellular matrix and physiological activity-related genes, indicating that acoustic stimulation is an important tool in regulating the stem cells' differentiation processes in cell therapy.
ACTA BIOMATERIALIA
(2022)
Article
Cell & Tissue Engineering
Jingran Zhang, Guang Shi, Junjie Pang, Xing Zhu, Qingcai Feng, Jie Na, Wenbin Ma, Dan Liu, Songyang Zhou
Summary: Induced crotonylation in hESCs resulted in differentiation into the endodermal lineage. This induced modification led to transcriptomic changes and decreased glycolysis in hESCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Erika Cosset, Youssef Hibaoui, Sten Ilmjarv, Pierre-Yves Dietrich, Caroline Tapparel, Karl-Heinz Krause
Summary: This study presents a standardized protocol for differentiating human embryonic stem cells into engineered neural tissue enriched with motor neurons, and highlights the role of motor neurons in poliovirus infection within these tissues. The research also reveals the molecular events occurring during PV-3 infection in the motor neurons and emphasizes the modulation of a set of genes involved in the EGR-EP300 complex.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Neurosciences
Carla Belmonte-Mateos, Cristina Pujades
Summary: The central nervous system exhibits a wide diversity of neurons with specific cell types and proportions in the appropriate locations. The coordination of proliferation and differentiation is crucial for generating brains with specific size and cell composition. Understanding how cell diversity arises from pluripotent progenitor cells and how progenitor potential changes over time is an important focus in developmental neurobiology.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Mohaddeseh Sadat Alavi, Sajad Sahab Negah, Ahmad Ghorbani, Azar Hosseini, Hamid R. Sadeghnia
Summary: The study demonstrates that LEV can enhance the proliferation of rat embryonic neural stem cells and promote neuronal differentiation mainly through an NMDA receptor-mediated mechanism.
Article
Biochemistry & Molecular Biology
Shotaro Kawano, Masayuki Baba, Hotaka Fukushima, Daiki Miura, Hitoshi Hashimoto, Takanobu Nakazawa
Summary: This study analyzed the role of ANK2 in the developing mouse cerebral cortex and found that ANK2 regulates neural stem cell differentiation and neuronal migration. ANK2 haploinsufficiency may impair neural development and increase the risk of ASD in patients.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Masahiro Otsu, Zubair Ahmed, Daniel Fulton
Summary: A simple method was developed to generate immature oligodendrocyte lineage cells from mouse ESC-derived neural stem cells, which showed potential for differentiation into mature OPC under specific conditions. Interestingly, these cells retained multipotentiality and could differentiate into neurons or astrocytes. However, their efficiency in myelinating axons was lower compared to primary OPC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Alexandros Sountoulidis, Sergio Marco Salas, Emelie Braun, Christophe Avenel, Joseph Bergenstrahle, Jonas Theelke, Marco Vicari, Paulo Czarnewski, Andreas Liontos, Xesus Abalo, Zaneta Andrusivova, Reza Mirzazadeh, Michaela Asp, Xiaofei Li, Lijuan Hu, Sanem Sariyar, Anna Martinez Casals, Burcu Ayoglu, Alexandra Firsova, Jakob Michaelsson, Emma Lundberg, Carolina Waehlby, Erik Sundstroem, Sten Linnarsson, Joakim Lundeberg, Mats Nilsson, Christos Samakovlis
Summary: Sountoulidis et al. have created a spatial gene expression atlas of the human embryonic lung during the first trimester of gestation, identifying 83 cell states and developmental trajectories. They integrated single-cell RNA sequencing and spatially resolved transcriptomics, providing an open platform for interactive exploration. The atlas can serve as a reference for further research and understanding of pulmonary diseases.
NATURE CELL BIOLOGY
(2023)
Article
Obstetrics & Gynecology
Salomeh Salari, Eli Y. Adashi, Laura Keller, Timothy R. B. Johnson, Gary D. Smith
Summary: Human embryonic stem cells (hESCs) have shown great potential in disease modeling, understanding early human development, pharmaceutical screening, disease treatment, and regenerative medicine. This article discusses the current regulatory environment in the US and presents anonymized embryo donor statements to personalize the decision-making process for hESC derivation. The use of preimplantation genetic-tested and disease-specific hESCs provides valuable insights into early human genetics and developmental biology. Future applications of hESCs and other pluripotent stem cells in reproductive medicine are also discussed.
FERTILITY AND STERILITY
(2023)
Article
Cell Biology
Jingsi Yang, Nan Ding, Dandan Zhao, Yunsheng Yu, Chunlai Shao, Xuan Ni, Zhen-Ao Zhao, Zhen Li, Jianquan Chen, Zheng Ying, Miao Yu, Wei Lei, Shijun Hu
Summary: The study developed a new strategy of using EBSS treatment to mimic in vivo starvation event, promoting the maturation of hESC-CMs. Intermittent starvation through EBSS significantly enhanced structural, metabolic, and electrophysiological maturity of hESC-CMs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Environmental
Timothy Hua, Sonia Kiran, Yan Li, Qing-Xiang Amy Sang
Summary: This study explores the adverse effects of polystyrene microplastics (PS-MPs) on the human brain, showing that their exposure can affect embryonic brain-like tissue development and gene expression.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Victoria C. de Leeuw, Jeroen L. A. Pennings, Ellen V. S. Hessel, Aldert H. Piersma
Summary: The article discusses the animal-free assessment of compound-induced developmental neurotoxicity based on batteries of multiple in vitro tests, emphasizing the importance of defining the biological domain and test combinations. The study highlights the use of ESTn in predicting the neurotoxicity of compounds during development, revealing insights into cell differentiation and gene expression patterns of neural cell types through key biomarker genes.
Article
Cell Biology
Anny W. Robert, Marco A. Stimamiglio
Summary: This study evaluated the effects of conditioned medium (CM) collected at different time points during in vitro cardiomyogenesis and found that CM obtained at the initial time points could promote cell proliferation. However, the secretome derived from cardiac committed cells during cardiomyogenesis was unable to improve angiogenesis or migration in endothelial cells, or to stimulate the differentiation of cardioblasts.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Guan-Yu Zhang, Zhu-Man Lv, Hao-Xin Ma, Yu Chen, Yuan Yuan, Ping-Xin Sun, Yu-Qi Feng, Ya-Wen Li, Wen-Jie Lu, Yu-Dong Yang, Cheng Yang, Xin-Lu Yu, Chao Wang, Shu-Long Liang, Ming-Liang Zhang, Hui-Liang Li, Wen-Lin Li
Summary: This study has developed an efficient method to induce pMN progenitors from human embryonic stem cells. These pMN progenitors can be stably maintained for multiple passages and retain their ability to rapidly produce spinal motor neurons and oligodendrocytes. These self-renewing pMN progenitors may have important clinical implications for the treatment of spinal cord injury and demyelinating disorders.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Ecology
Tae Young Ryu, Kwangho Kim, Tae-Su Han, Mi-Ok Lee, Jinkwon Lee, Jinhyeon Choi, Kwang Bo Jung, Eun-Jeong Jeong, Da Mi An, Cho-Rok Jung, Jung Hwa Lim, Jaeeun Jung, Kunhyang Park, Moo-Seung Lee, Mi-Young Kim, Soo Jin Oh, Keun Hur, Ryuji Hamamoto, Doo-Sang Park, Dae-Soo Kim, Mi-Young Son, Hyun-Soo Cho
Summary: The human microbiome has been found to play a crucial role in regulating colorectal cancer growth through the modulation of EHMT2 protein levels and TNFAIP1 gene expression. This study suggests that propionate and EHMT2 inhibitors may serve as therapeutic targets for colon cancer treatment, and there may be synergistic effects between EHMT2 inhibitors and the microbiome in CRC treatment.
Article
Oncology
Kunhyang Park, Kwangho Kim, Tae Young Ryu, Jinkwon Lee, Moo-Seung Lee, Mi-Young Son, Seon-Jin Lee, Young-Jun Park, Hyun-Soo Cho, Dae-Soo Kim
Summary: The present study used Gene Ontology analysis to investigate the biological responses of lung epithelial cells and fibroblasts following exposure to PM. The results suggested that PM may affect cell condition and may be associated with several human diseases.
MOLECULAR MEDICINE REPORTS
(2022)
Article
Cell Biology
Jung Hwa Lim, Dae Hun Kim, Kyung Hee Noh, Cho-Rok Jung, Hyun Mi Kang
Summary: This study identified epidermal progenitor cells that possess unlimited proliferation and multipotent differentiation capacity. These cells can form organotypic skin models and mimic the morphologic and biochemical processes of human epidermis. The proliferation and differentiation of these cells rely on the expression of Integrin beta 1 (ITGB1). Furthermore, the in vitro and in vivo wound healing capacity of these cells was examined.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Hyun Mi Kang, Dae Hun Kim, Mijin Kim, Yoohong Min, Bohyeon Jeong, Kyung Hee Noh, Da Yong Lee, Hyun-Soo Cho, Nam-Soon Kim, Cho-Rok Jung, Jung Hwa Lim
Summary: This study discovered that the FBXL17-SPAST pathway is involved in the pathogenicity of hereditary spastic paraplegia (HSP) through loss of function and quantitative regulation. Targeting FBXL17 could provide new insights into HSP therapeutics.
CELL AND BIOSCIENCE
(2022)
Article
Engineering, Biomedical
Seul-Gi Lee, Ye-Ji Kim, Mi-Young Son, Min-Seok Oh, Jin Kim, Bokyeong Ryu, Kyu-Ree Kang, Jieun Baek, Gujin Chung, Dong Ho Woo, C-Yoon Kim, Hyung Min Chung
Summary: In response to the growing demand for 3D culture, we studied heart organoids. By creating self-organized HOs using human iPSCs, we demonstrated their ability to mimic cardiac structures and verified their mechanical/electrophysiological features through multiple analyses. The cardiovascular characteristics of HOs were maintained after in vivo transplantation.
Article
Multidisciplinary Sciences
Jeongmin Ha, Bum Suk Kim, Byungkuk Min, Juhyeon Nam, Jae-Geun Lee, Minhyung Lee, Byoung-Ha Yoon, Yoon Ha Choi, Ilkyun Im, Jung Sun Park, Hyosun Choi, Areum Baek, Sang Mi Cho, Mi-Ok Lee, Ki-Hoan Nam, Ji Young Mun, Mirang Kim, Seon-Young Kim, Mi Young Son, Yong-Kook Kang, Jeong-Soo Lee, Jong Kyoung Kim, Janghwan Kim
Summary: The cytoskeletal linker protein desmoplakin (Dsp) plays a crucial role in both in vitro cellular reprogramming and tissue regeneration. Knockdown of Dsp impairs the formation of intermediate cells during cellular reprogramming and tissue regeneration. This study suggests a potential evolutionary link mediated by Dsp between cellular reprogramming in mammals and tissue regeneration in lower vertebrates, and the intermediate cells may provide alternative approaches for mammalian regenerative therapy.
Article
Gastroenterology & Hepatology
Hana Lee, Kwang Bo Jung, Ohman Kwon, Ye Seul Son, Eunho Choi, Won Dong Yu, Naeun Son, Jun Hyoung Jeon, Hana Jo, Haneol Yang, Yeong Rak Son, Chan-Seok Yun, Hyun-Soo Cho, Sang Kyu Kim, Dae-Soo Kim, Doo-Sang Park, Mi-Young Son
Summary: This study investigates the modulatory capacity of a newly isolated probiotic strain (DS0384) on early intestinal development. They found that DS0384 accelerated the maturation of fetal intestines and promoted intestinal stem cell proliferation by secreting a specific metabolite (NCG). The results suggest that DS0384 has potential probiotic properties and therapeutic applications for early gut development disorders.
Article
Biochemistry & Molecular Biology
Hae-Ri Lee, Soo Kim, Sungho Shin, Seon-Yeong Jeong, Dae-Won Lee, Sun-Ung Lim, Ji Yeon Kang, Mi-Young Son, Cheolju Lee, Kyung-Rok Yu, Myungshin Kim, Il-Hoan Oh
Summary: In this study, the transition from primary MSCs to iMSCs was characterized by increased proliferative activity and variations in differentiation potential. iMSCs exhibited unique gene and protein expression patterns, resembling pericyte-like cells, and showed enhanced self-renewal and immune suppression capabilities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Ilkyu Park, Nakyoung Kim, Sugi Lee, Kunhyang Park, Mi-Young Son, Hyun-Soo Cho, Dae-Soo Kim
Summary: The timely diagnosis of different stages in NAFLD is crucial for disease treatment and reversal. In this study, we identified differentially expressed genes in NAFLD patients compared to healthy controls and determined their predictive power. We also found significant differential expression of candidate genes in liver cancer that may influence patient survival.
Article
Engineering, Biomedical
Hyunah Ahn, Younghak Cho, Geun-Tae Yun, Kwang Bo Jung, Wonji Jeong, Yesol Kim, Mi-Young Son, Eunjung Lee, Sung Gap Im, Hee-Tae Jung
Summary: This study presents a hierarchical structure composed of nano-pillars on micro-wrinkles for enhancing cardiomyocyte maturation. The responses of cardiomyocytes on hierarchical topography are systematically investigated. The combination of nano-pillars and micro-wrinkles significantly enhances the maturation of cardiomyocytes.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Kwangho Kim, Tae Young Ryu, Jinkwon Lee, Mi-Young Son, Dae-Soo Kim, Sang Kyum Kim, Hyun-Soo Cho
Summary: This study identified EHMT1 as an overexpressed gene in colorectal cancer (CRC) and demonstrated that knocking down EHMT1 expression suppressed cell growth and induced apoptosis in CRC cell lines. Further analysis revealed that EHMT1 regulates cell apoptosis through the CHOP gene. These findings suggest EHMT1 as a potential therapeutic target for CRC treatment.
MOLECULES AND CELLS
(2022)
Article
Biochemistry & Molecular Biology
Seung-Ho Park, Sung-Jin Yoon, Song Choi, Jaeeun Jung, Jun-Young Park, Young-Ho Park, Jinho Seo, Jungwoon Lee, Moo-Seung Lee, Seon-Jin Lee, Mi-Young Son, Young-Lai Cho, Jang-Seong Kim, Hyo Jin Lee, Jinyoung Jeong, Dae-Soo Kim, Young-Jun Park
Summary: The study reveals that exposure to particulate matter pollutants can induce immune cells to secrete HBEGF, leading to a more invasive state in cancer cells. This finding helps uncover the mechanism by which PM promotes cancer metastasis.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jongjin Park, Dong Gwang Lee, Na Geum Lee, Min-Gi Kwon, Yeon Sung Son, Mi-Young Son, Kwang-Hee Bae, Jangwook Lee, Jong-Gil Park, Nam-Kyung Lee, Jeong-Ki Min
Summary: In this study, researchers developed a monoclonal antibody K312 that can distinguish undifferentiated pluripotent stem cells (PSCs) from differentiated PSC progeny. By targeting with K312, pluripotent cells can be depleted from differentiated PSC progeny, effectively preventing teratoma formation.
Article
Cell & Tissue Engineering
Ohman Kwon, Won Dong Yu, Ye Seul Son, Kwang Bo Jung, Hana Lee, Mi-Young Son
Summary: This study develops a reliable culture method for 3D intestinal spheroids (InSexp) that can expand and maintain human intestinal stem cells (ISCs). By activating the WNT pathway, the InSexp can rapidly expand and be passaged. These findings contribute to the development of scalable culture systems for studying ISC biology.
INTERNATIONAL JOURNAL OF STEM CELLS
(2022)