Article
Biochemistry & Molecular Biology
Tanyaporn Keratibumrungpong, Warunee Srisuthtayanont, Orawan Wanachewin, Jeerawan Klangjorhor, Thanyaluck Phitak, Peraphan Pothacharoen, Thuzar Hla Shwe, Prachya Kongtawelert
Summary: This study investigates the effect of sesame seed phytochemical sesamin on physiological and pathological angiogenesis. Sesamin significantly inhibits VEGFA-induced pathological angiogenesis and reduces vascular branching, endothelial cell proliferation, and migration. It also inhibits the activation of Src and FAK signaling proteins and reduces the expression of angiogenesis-related genes, suggesting its potential use in the prevention or treatment of diseases with excessive angiogenesis.
Article
Biochemistry & Molecular Biology
Olga M. Palomino, Veronica Giordani, Julie Chowen, Soledad Fernandez Alfonso, Luis Goya
Summary: Oxidative stress can lead to endothelial dysfunction and cardiovascular disease. Free fatty acids, particularly olive oil fatty acids, have beneficial effects on cardiovascular risk factors. Physiological concentrations of palmitic and oleic acids may protect vascular endothelium against oxidative stress.
Article
Chemistry, Medicinal
Hyo-Geun Lee, Hyun-Soo Kim, Hyesuck An, Kyunghwa Baek, Jeong Min Lee, Mi-Jin Yim, Seok-Chun Ko, Ji-Yul Kim, Gun-Woo Oh, Jun-Geon Je, Dae-Sung Lee, You-Jin Jeon
Summary: The aim of this study was to assess the potential hypertensive effects of the IGTGIPGIW peptide purified from Hippocampus abdominalis alcalase hydrolysate (HA) for application in the functional food industry. The antihypertensive effects of IGTGIPGIW were studied in vitro using endothelial cells, and its vasorelaxation effect was evaluated using a fluorescent stain. Furthermore, the study also demonstrated the antihypertensive activity of IGTGIPGIW in a rat model in vivo. These findings suggest the suitability of IGTGIPGIW for application in the functional food industry.
Article
Biochemistry & Molecular Biology
Paola Faraoni, Lorenzo Cecchi, Maria Bellumori, Alessio Gnerucci, Francesco Ranaldi, Nadia Mulinacci
Summary: Pate, a by-product of olive oil production, is rich in phenolic compounds and can be used as a food ingredient to reduce environmental impact and promote circular economy. The effects of a hydroalcoholic extract of pate were evaluated in AGS human cell line, showing no significant impact on cell viability, cycle, proliferation, and migration. The extract also counteracted the enzymatic activity alterations caused by oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ying Zhang, Pham Ngoc Khoi, Bangrong Cai, Dhiraj Kumar Sah, Young-Do Jung
Summary: This study reveals a novel effect of sulforaphane (SFN) in stimulating eNOS activity in cells by regulating NO bioavailability, suggesting its potential as a promising therapeutic candidate for preventing endothelial dysfunction, atherosclerosis, and other cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Martina Bartolomei, Carlotta Bollati, Maria Bellumori, Lorenzo Cecchi, Ivan Cruz-Chamorro, Guillermo Santos-Sanchez, Giulia Ranaldi, Simonetta Ferruzza, Yula Sambuy, Anna Arnoldi, Nadia Mulinacci, Carmen Lammi
Summary: This study provides a comprehensive characterization of the antioxidant properties of phenolic extract (BUO) obtained from extra virgin olive oil, cultivated in Tuscany, Italy. The findings show that BUO extract can reduce harmful substances produced in cells after oxidative stress and demonstrate selective trans-epithelial transport of some phenolic components in differentiated Caco-2 cells, a mature enterocyte model.
Article
Chemistry, Multidisciplinary
Arrigo F. G. Cicero, Federica Fogacci, Antonio Di Micoli, Maddalena Veronesi, Elisa Grandi, Claudio Borghi
Summary: This study evaluated the effect of dietary supplementation with highly standardized polyphenols derived from Coratina Olive on the plasma cholesterol levels of hypercholesterolemic individuals. Results showed significant improvement in several blood markers with this supplementation, indicating its potential in the prevention and treatment of metabolic syndrome.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Cinzia Benincasa, Chiara La Torre, Alessia Fazio, Enzo Perri, Maria Cristina Caroleo, Pierluigi Plastina, Erika Cione
Summary: Recent study identified the presence of tyrosyl oleate, a subclass of lipophenols, in olive oil with anti-inflammatory, antioxidant, and tissue regenerating properties.
Tyrosyl oleate was quantitatively determined and its effects on skin cells were investigated, showing improved cell viability and antioxidant activity.
Interestingly, tyrosyl oleate concentration was found to be higher in lower quality oils, likely due to processing and rancidity, suggesting a correlation between concentration and oil quality.
Article
Biochemistry & Molecular Biology
Costas S. Papageorgiou, Paraskevi Lyri, Ioanna Xintaropoulou, Ioannis Diamantopoulos, Dimitris P. Zagklis, Christakis A. Paraskeva
Summary: The aim of this study was to explore high-yield production of hydroxytyrosol, a phenolic compound with high antioxidant capacity. Olive leaves were chosen as the feedstock due to their significant oleuropein content. Various techniques were used, including drying, mechanical pretreatment, solid-liquid extraction, hydrolysis, and extraction with ethyl acetate. The hydroxytyrosol content was significantly increased through these steps.
Article
Pharmacology & Pharmacy
Maria Cristina Caroleo, Pierluigi Plastina, Alessia Fazio, Chiara La Torre, Fabrizio Manetti, Erika Cione
Summary: The study focused on the potential effects of natural lipophenols on insulin secretion in type 2 diabetes mellitus, and their role in modulating the FFAR1 receptor. The findings suggest that these compounds may play a role in the anti-diabetic properties associated with olive oil consumption.
Article
Chemistry, Medicinal
Nikolaos Iakovis, Ignatios Ikonomidis, Ioanna Andreadou, Andrew Xanthopoulos, Aikaterini Chamaidi, Nikolaos Chrysakis, Grigorios Giamouzis, John Skoularigis, Ioulia Tseti, Filippos Triposkiadis
Summary: Short-term use of OE enriched with HT showed a trend towards improvement of LV diastolic function and arterial elastic properties in chronic CAD patients, suggesting potential benefits for cardiovascular function.
JOURNAL OF MEDICINAL FOOD
(2023)
Article
Chemistry, Analytical
Konstantina Papastavropoulou, Ioannis N. Pasias, Elissavet Dotsika, Emel Oz, Fatih Oz, Charalampos Proestos
Summary: The study evaluated the key quality characteristics of olive oil, focusing on biophenolic content and its impact on health and the contribution of various parameters to health claims. The findings showed that the biophenol contents in olive oil and its health claims were influenced by various parameters.
Article
Nutrition & Dietetics
Fernando Rivero-Pino, Elena Grao-Cruces, Soledad Lopez-Enriquez, Gonzalo Alba, Elvira Marquez-Paradas, Carmen M. M. Claro-Cala, Consuelo Santa-Maria, Sergio Montserrat-de la Paz
Summary: Inflammation is the defense mechanism against harmful stimuli and persistent neuroinflammation can lead to neurodegenerative disorders.In this study, the phenolic fraction from virgin olive oil was found to modulate the activation of primary human neutrophils stimulated by beta-amyloid, preventing neutrophil activation and down-regulating pro-inflammatory cytokine gene expression.
Article
Biochemistry & Molecular Biology
Nereida L. Corrales, Fernando Sevillano, Rosa Escudero, Gonzalo G. Mateos, David Menoyo
Summary: The experiment aimed to investigate the substitution effect of a liquid rich in hydroxytyrosol (HT) obtained from an olive oil by-product for vitamin E (VE) in poultry diets. The results showed that the olive oil by-product, which is rich in HT, can be used as a strategy to spare VE in broiler diets once the nutritional requirements of the birds in VE are satisfied.
Article
Food Science & Technology
Yan Yi Qi, Tian Gong, Peng Tao Zhao, Yong Jie Niu, Yong Yuan Hu, Ching Yuan Hu, Shuai Zhang, Yong Hong Meng
Summary: The use of antioxidants in easily oxidizable food oils has garnered attention from the food industry. Hydroxytyrosol, a potent antioxidant, was modified into hydroxytyrosyl oleate to enhance its solubility in oil, and its effectiveness as an antioxidant in bulk oil was thoroughly evaluated. Hydroxytyrosyl oleate exhibited moderate scavenging of free radicals, reduction of Fe3+, and adsorption of oxygen. Additionally, it demonstrated better performance in delaying peroxide value generation, acid value increase, and hydroperoxide production during accelerated oxidation compared to hydroxytyrosol (HT), tertiary butylhydroquinone (TBHQ), butylated hydroxytoluene (BHT), and Vitamin E (VE). Furthermore, hydroxytyrosyl oleate effectively prevented fatty acid oxidation and the development of undesirable odors in olive oil. These findings suggest that hydroxytyrosyl oleate is a safe antioxidant that can be widely applied in foods with high content of polyunsaturated fatty acids to reduce lipid oxidation and food spoilage.
Article
Environmental Sciences
Magdalena Mititelu, Denisa Ioana Udeanu, Anca Oana Docea, Aristidis Tsatsakis, Daniela Calina, Andreea Letitia Arsene, Mirela Nedelescu, Sorinel Marius Neacsu, Bruno Stefan Velescu, Manuela Ghica
Summary: The release of heavy metals into the natural environment poses problems for ecosystems and human health. Honey can contain heavy metals from agricultural or industrial sources, and consuming contaminated honey can lead to serious health problems. This study aimed to determine the concentrations of heavy metals in honey and soil and assess the associated risks.
ENVIRONMENTAL RESEARCH
(2023)
Review
Food Science & Technology
Ronald Neil Kostoff, Michael Brandon Briggs, Darja Kanduc, Saikat Dewanjee, Ramesh Kandimalla, Yehuda Shoenfeld, Alan L. Porter, Aristidis Tsatsakis
Summary: The severe complications of COVID-19 are a result of the individual's dysfunctional immune response to the initial SARS-CoV-2 infection. Multiple toxic stressors and behaviors contribute to immune system dysfunction which the virus exploits to cause COVID-19. This study identifies eighty modifiable contributing factors that link directly to COVID-19, and shows how each factor contributes to immune system dysfunction, inflammation, coagulation, neural damage, and neurodegeneration. It emphasizes the importance of addressing and eliminating these factors in combatting COVID-19 and similar diseases caused by viral variants.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Review
Medicine, Research & Experimental
Seyed Sajjad Babaeimarzangou, Himasadat Zaker, Ehsan Soleimannezhadbari, Naeimeh Shamsi Gamchi, Masoud Kazeminia, Shima Tarighi, Homayon Seyedian, Aristidis Tsatsakis, Demetrios A. Spandidos, Denisa Margina
Summary: Zoonotic viral diseases have become a major threat to global public health and economies in recent times. The lack of vaccines and effective antiviral drugs against most of these viruses increases the risk of new outbreaks. This review provides an update on the progress of vaccine development in clinical trials for various zoonotic viruses.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Toxicology
Michael Aschner, Anatoly V. Skalny, Rongzhu Lu, Abel Santamaria, Ji-Chang Zhou, Tao Ke, Mikhail Yu. Karganov, Aristides Tsatsakis, Kirill S. Golokhvast, Aaron B. Bowman, Alexey A. Tinkov
Summary: This review summarizes the existing data on the effects of toxic metals on HIF-1 signaling and its underlying mechanisms, with a special focus on the prooxidant effect of metals. The effect of metals on HIF-1 pathway varies depending on the cell type, ranging from down-regulation to up-regulation. Inhibition of HIF-1 signaling may contribute to hypoxic damage in cells, while metal-induced activation may promote tumor growth and contribute to the carcinogenic effects of heavy metals.
ARCHIVES OF TOXICOLOGY
(2023)
Letter
Toxicology
Gio B. Gori, Michael Aschner, Christopher J. Borgert, Samuel M. Cohen, Daniel R. Dietrich, Corrado L. Galli, Helmut Greim, John S. Heslop-Harrison, Sam Kacew, Norbert E. Kaminski, James E. Klaunig, Hans W. J. Marquardt, Olavi Pelkonen, Ruth Roberts, Kai M. Savolainen, Aristidis Tsatsakis, Hiroshi Yamazaki
Summary: The 1958 Delaney amendment banned food additives causing cancer in animals through appropriate tests. However, chronic animal cancer tests have proven to be inconsistent and unreliable in predicting cancer hazards in humans. This poses an existential crisis for regulatory agencies that have relied on such tests to formulate regulations for alleged cancer causing agents.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Toxicology
Viorica Dinca, Anca Oana Docea, Andrei Ioan Drocas, Taxiarchis Konstantinos Nikolouzakis, Polychronis D. Stivaktakis, Dragana Nikitovic, Kirill S. Golokhvast, Antonio F. Hernandez, Daniela Calina, Aristidis Tsatsakis
Summary: The current risk assessment approach for chemicals fails to consider real-life exposure scenarios. The exposure to chemical mixtures in everyday life has become a concern in recent years. This study investigated the long-term effects of exposure to a mixture of 13 chemicals in adult rats and found dose-dependent changes in all examined organs. The main organs involved in biotransformation and clearance consistently presented histopathological changes. Exposure to very low doses of the tested mixture for 18 months induced histopathological lesions and cytotoxic effects in a dose and tissue-dependent manner.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Environmental Sciences
K. Yu Maksimovich, R. Yu Dudko, E. I. Shatalova, A. K. Tsakalof, A. M. Tsatsakis, K. S. Golokhvast, E. A. Novikov
Summary: The detrimental environmental consequences caused by intensification of crop cultivation can be prevented through monitoring of specific biological indicators. This study focused on the impact of crop type and cultivation intensity on the community of ground beetles in the forest-steppe of Western Siberia. The ground beetles' community showed a high evenness of species distribution across the agroecosystems. Differences in ground beetle distribution were observed between wheat and corn crops. The use of bioindicators allowed for assessing the environmental sustainability of the agricultural environment and provided insights for ecologically directed correction of agrotechnological operations in agroecosystem management.
ENVIRONMENTAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Daniela-Madalina Ciuca Anghel, Ana-Maria Vlasceanu, Gheorghe Tudor, Anne-Marie Ciobanu, Miriana Stan, Gabriela Viorela Nitescu, Dimitra Florou, Aristidis M. Tsatsakis, Daniela Luiza Baconi
Summary: The aim of our study was to evaluate the differences among different groups of drug users, focusing on hematological, biochemical, urinary, and cardiological aspects. A retrospective study was conducted on 2572 hospitalized patients in Bucharest, Romania from 2015 to 2021. The study group was characterized based on demographic data, personal history/co-morbidities, consumption history, and substitution treatment. The analysis of medical records revealed correlations between GGT levels and alcohol intake as well as contagious diseases, a decrease in MCH and MCHC levels across the entire study group, and correlations between specific cardiological events and certain substance groups. The study provides important information on different types of drug users and supports future research on the chemical structures of consumed compounds.
Review
Biochemistry & Molecular Biology
Evangelia Sarandi, Sabine Krueger-Krasagakis, Dimitris Tsoukalas, Polytimi Sidiropoulou, George Evangelou, Maria Sifaki, Gottfried Rudofsky, Nikolaos Drakoulis, Aristidis Tsatsakis
Summary: Psoriasis is a common inflammatory disease that mainly affects the skin and can be associated with comorbidities such as psoriatic arthritis, Crohn's disease, metabolic syndrome, and cardiovascular disease. The development of psoriasis involves complex interactions between keratinocytes, T helper cells, and immune cells in the skin. Understanding the metabolic reprogramming of these cells in psoriatic skin can provide insights into novel diagnostic and therapeutic targets. The dysregulation of metabolic pathways, such as glycolysis, TCA cycle, amino acid metabolism, and fatty acid metabolism, plays a significant role in psoriasis. Targeting these pathways and restoring metabolic imbalances may present promising therapeutic opportunities for long-term management of psoriasis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Food Science & Technology
Eleftheria Hatzidaki, Marina Pagkalou, Ioanna Katsikantami, Elena Vakonaki, Matthaios Kavvalakis, Aristidis M. Tsatsakis, Manolis N. Tzatzarakis
Summary: Human milk is the healthiest option for newborns, but infant formula is a valuable alternative in certain circumstances. Ensuring pollutant-free composition, infant formulas are regulated and monitored for safe exposure. While legislation varies across countries, standard policies for protecting vulnerable infants are in place. This article presents current regulations and directives for limiting endocrine-disrupting chemicals and persistent organic pollutants in infant formulas, highlighting the need for limited risk assessment studies to assess the health risks for infants from dietary exposure to pollutants.
Article
Biochemistry & Molecular Biology
Betul Danisman, Betul Cicek, Serkan Yildirim, Ismail Bolat, Deniz Kantar, Kirill S. Golokhvast, Dragana Nikitovic, Aristidis Tsatsakis, Ali Taghizadehghalehjoughi
Summary: This study aimed to investigate the impact of carnosic acid (CA) on indomethacin (IND)-induced gastric ulceration. The results showed that CA pretreatment attenuated both gross morphological lesions and histopathological alterations induced by IND, and it achieved gastroprotective effects by reducing oxidative stress and suppressing inflammation.
Review
Environmental Sciences
Efthimios Dardiotis, Panagiotis Skouras, Orfeas-Petros Varvarelis, Athina-Maria Aloizou, Antonio F. Hernandez, Ioannis Liampas, Dimitrios Rikos, Metaxia Dastamani, Kirill S. Golokhvast, Dimitrios P. Bogdanos, Aristidis Tsatsakis, Vasileios Siokas, Panayiotis D. Mitsias, Georgios M. Hadjigeorgiou
Summary: Pesticides can cause tremors and other diseases related to the nervous system. More research and preventive measures are needed to protect exposed populations from potential disabilities and social drawbacks.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Paraskevi-Maria Nechalioti, Thomas Karampatzakis, Robin Mesnage, Michael N. Antoniou, Mariam Ibragim, Aristidis Tsatsakis, Anca Oana Docea, Charitini Nepka, Demetrios Kouretas
Summary: The wide-scale emergence of glyphosate-resistant weeds has caused an increase in the application of herbicide mixtures containing glyphosate plus dicamba or glyphosate plus 2,4-D. This raises concerns about the environmental and health risks from exposure to a mixture of herbicide active ingredients. The study evaluated the hepatotoxic effects of perinatal exposure to glyphosate alone or in combination with 2,4-D and dicamba on Wistar rats. The findings highlight the importance of assessing the combined effects of chemical pollutants at safe individual doses.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Betul Cicek, Betul Danisman, Serkan Yildirim, Neslihan Yuce, Dragana Nikitovic, Ismail Bolat, Mehmet Kuzucu, Ertugrul Ceyran, Ebru Bardas, Kirill S. Golokhvast, Aristidis Tsatsakis, Ali Taghizadehghalehjoughi
Summary: SN berry extract has protective effects against IND-induced gastric ulcers in rats by reducing oxidative damage and inflammation in the gastric mucosa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Correction
Medicine, Research & Experimental
Felician Stancioiu, Georgios Z. Papadakis, George Lazopoulos, Demetrios A. Spandidos, Aristidis Tsatsakis, Marius Floroiu, Corin Badiu
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)