期刊
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 42, 期 34, 页码 21697-21703出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2017.07.126
关键词
Escherichia coli; Hydrogenases; Glycerol, glucose and formate utilization; Bioenergy and biohydrogen; pH
资金
- Armenian National Science and Education Fund (ANSEF, USA) [Biotech 4184]
- State Committee on Science, Ministry of Education and Science of Armenia [15T-1F123]
Biohydrogen production by Escherichia coli during fermentation of the mixture of glycerol, glucose and formate at different pH values was studied. Employing mutants lacking large subunits of different hydrogenases (Hyd), it was reported that, at pH 7.5, H-2 production was produced except in a hyaB hybC hycE triple mutant, thus suggesting compensatory H-2 -producing functions of the Hyd enzymes. Activity of Hyd-4 was revealed in glucose assays at pH 7.5 in the triple mutant whereby 62% of the wild type level of H-2 production was derived from Hyd-4. In formate assays, it was shown, that, first, the hyaB hybC double mutant had a H-2 production similar to 3 fold higher than wild type, indicating that Hyd-1 and Hyd-2 oxidize H-2, and second, that at pH 5.5, Hyd-4 and Hyd-3 were responsible for H-2 production. These findings are significant when applying various carbon sources such as sugars, alcohol and organic acids for biohydrogen production. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据